A Combined Method of Protein Extraction from Unorthodox Plant Samples for Proteomics

2020 ◽  
Vol 18 ◽  
Author(s):  
Can Yilmaz ◽  
Mesude Iscan
Keyword(s):  
Protein Extraction ◽  
New Method ◽  
High Tech ◽  
Capillary Chromatography ◽  
Combined Method ◽  
Molecular Systems ◽  
Yield And Quality ◽  
Extraction And Purification ◽  
Sds Page

Aim:: This study aimed to generate an improved method of protein extraction and purification from plant tissues containing very high amounts of phenolic compounds and other interfering biomolecules. Background:: Protein extraction at proteomic studies on some plant species including conifers is challenging and the yield and quality is unpredictable. Objective:: Two popular protocols were combined with each other to construct a novel one with enhanced abilities to produce higher purity of samples compatible for high precision molecular systems and analysis. Method:: The new method was compared with the other two for their efficiencies in classical SDS-PAGE, 2-DE and capillary chromatography applications. Results:: All three methods were comparable in SDS-PAGE procedure; however, only the new method created acceptable gel images in 2-DE. Bioanalyzer results, also, demonstrated that the new method provided protein samples pure enough to be used in capillary chromatography with 2 times more peaks in electropherograms with lower noise and higher total relative protein concentrations closest to the applied amount. Conclusion:: The new combined method is a successful alternative for plant proteomicists with higher yield and quality of proteins from recalcitrant tissues. Other:: The new method could be preferred, especially, for high-tech, sensitive proteomic analysis.

Processability predictions for mechanically recycled blends of linear polymers

2020 ◽  
Vol 40 (9) ◽  
pp. 771-781
Author(s):  
Janne van Gisbergen ◽  
Jaap den Doelder
Keyword(s):  
Molecular Weight ◽  
Circular Economy ◽  
Melt Flow Index ◽  
Building Blocks ◽  
Experimental Designs ◽  
New Method ◽  
Flow Index ◽  
Linear Polymers ◽  
Combined Method

AbstractRecycling of thermoplastic polymers is an important element of sustainable circular economy practices. The quality of mechanically recycled polymers is a concern. A method is presented to predict the structure and processability of recycled blends of polymers based on processability knowledge of their virgin precursor components. Blending rules at molecular weight distribution level are well established and form the foundation of the new method. Two essential fundamental building blocks are combined with this foundation. First, component and blend structure are related to viscosity via tube theories. Second, viscosity is related to melt flow index via a continuum mechanics approach. Emulator equations are built based on virtual experimental designs for fast forward and reverse calculations directly relating structure to viscosity and processability. The new combined method is compared with empirical blend rules, and shows important similarities and also clear quantitative differences. Finally, the new method is applied to practical recycling quality challenges.

scholarly journals Between retrogression and progression – a proposal for a new method to recreate the historical building layout of settlement units

2019 ◽  
Vol 51 (4) ◽  
pp. 215-229
Author(s):  
Jakub Kuna
Keyword(s):  
Cultural Landscapes ◽  
Residential Buildings ◽  
New Method ◽  
Combined Method ◽  
Mathematical Methods ◽  
Historical Building ◽  
Component Reliability ◽  
Main Research ◽  
Set Operations

Abstract The use of cartographic sources and methods are the basic tools of historical geography. One of the main research trends in this field is the analysis of the spatial layout and number of old settlement units. The confrontation of maps with historical data allows the drawing of a town’s area at a certain time to be studied. The retrogression (R) and progression (P) methods that are currently used are imperfect and the model created (map) is usually incomplete and its reliability is limited. In the author’s opinion, the joining of retrogression and progression (a new method; combined – K)1 increases the quality of cartographic reconstruction of natural and cultural landscapes. The use of basic mathematical methods from the scope of set operations means the component reliability of the researched cartographic model can be varied because the common part of the retrogression and progression cartographic model represents mutual verification of source data. Quantitative effectiveness assessments of retrogression (R), progression (P) and the combined method (K) can be made for countable elements (e.g. buildings). As part of the conducted study, the effectiveness of separate methods was calculated: R = 76% for retrogression, P = 59% for progression and K = R ∪ P = 85% for the combined method. The mutual verification of the methods (R ∩ P) included 45% of residential buildings. The author describes the proposition of a new method and the course of verification research.

scholarly journals Fine-tuned method to extract high purified proteins from the seagrass Halophila stipulacea to be used for proteome analyses

2021 ◽  
Author(s):  
Silvia Mazzuca ◽  
Amalia Piro ◽  
Vasileia Anagnostopoulou ◽  
Eugenia Apostolaki
Keyword(s):  
Trichloroacetic Acid ◽  
Protein Extraction ◽  
Protein Yield ◽  
Model Species ◽  
Halophila Stipulacea ◽  
Promising Tool ◽  
Sds Page ◽  
The Mediterranean ◽  
The Mediterranean Basin

The non-indigenous to the Mediterranean tropical seagrass Halophila stipulacea has the possibility to become more prevalent in the Mediterranean basin, exacerbated by the rapid increase of water temperature. Molecular profiling appears a promising tool to study the traits that render H. stipulacea tolerant and resilient and facilitate its rapid and vast geographical spread. Taking advantage from recent seagrass genomes sequencing, proteomics specialty has been applied to several seagrasses giving new insight on the biology and physiology of this group of angiosperms. Thus, it could be of interest to apply proteomics to H. stipulacea that it could be considered as a possible plant model species to study marine biological invasion. The first step to achieve this goal is to obtain high quality proteins from plant tissue. Tissue fixation and protein extraction protocol are the most challenging steps in proteomics . Here we report a fine-tuned procedure obtained by comparing protein yield from H. stipulacea plants frozen in liquid nitrogen or preserved in RNAlater and processed following two different extraction protocols. Higher protein yield have been extracted from the procedure that use the RNA later preserved plants, extracted with trichloroacetic acid in water followed by trichloroacetic acid in acetone, compared to those obtained from all other procedures. Protein purity of these samples have been tested by the separation in SDS-PAGE comfirming a better resolved profile of peptide bands suitable for a gel-based proteomics. Then, to assess the quality of proteins the m HPLC-ESI-MS/MS mass spectrometry analyses and bioinformatics have been performed.  Hundreds proteins have been identified against several seagrass genomic resources available at UniProt, NCBI, SeagrassDB and transcriptomic datasets, which were merged to form the first customized dataset useful for H. stipulacea proteomic investigations.

Evolution of yield and quality of sainfoin (Onobrychis viciifolia Scop.) in the spring growth cycle

Agronomie ◽  
2003 ◽  
Vol 23 (3) ◽  
pp. 193-201 ◽  
Author(s):  
Giorgio Borreani ◽  
Pier Giorgio Peiretti ◽  
Ernesto Tabacco
Keyword(s):  
Growth Cycle ◽  
Onobrychis Viciifolia ◽  
Yield And Quality

Орошение томата F1 Донской в открытом грунте Московской области

2019 ◽  
Author(s):  
D.I. Engalychev ◽  
N.A. Engalycheva ◽  
A.M. Menshikh
Keyword(s):  
Experimental Data ◽  
Agricultural Technology ◽  
Tomato Fruits ◽  
River Floodplain ◽  
Yield And Quality ◽  
Open Ground ◽  
Alluvial Meadow ◽  
Standard Production ◽  
Moscow River

Представлены экспериментальные данные о влиянии капельного орошения на урожайность и качество плодов томата при выращивании культуры в открытом грунте Московской области. На плодородных аллювиальных луговых почвах Москворецкой поймы при соблюдении агротехники без орошения в среднем за три года исследований в полевых условиях получена урожайность томата F1 Донской 31,9 т/га, с орошением 48,5 т/га, в т.ч. стандартной продукции 42,6 т/га.The article presents experimental data on the effect of drip irrigation on the yield and quality of tomato fruits when growing crops in open ground of the Moscow Region. On fertile alluvial meadow soils of the Moscow river floodplain, with the observance of agricultural technology without irrigation, the field yield of tomato hybrid F1 Donskoi on average for three years of research was 31.9 t/ha, with irrigation 48.5 t/ha, incl. standard production 42.6 t/ha.

Агрономическая эффективность промышленной (голландской) технологии возделывания картофеля

2018 ◽  
Author(s):  
C. Coy ◽  
A.V. Shuravilin ◽  
O.A. Zakharova
Keyword(s):  
Heavy Metals ◽  
Regression Analysis ◽  
Positive Response ◽  
Growth Yield ◽  
Correlation And Regression Analysis ◽  
Yield And Quality ◽  
Quality Of Products ◽  
High Degree ◽  
The Impact

Приведены результаты исследований по изучению влияния промышленной технологии возделывания картофеля на развитие, урожайность и качество продукции. Выявлена положительная реакция растений на подкормку K2SO4 в период посадки. Корреляционно-регрессионный анализ урожайности и качества клубней выявил высокую степень достоверности результатов опыта. Содержание нитратов и тяжелых металлов в клубнях было ниже допустимых величин.The results of studies on the impact of industrial technology of potato cultivation on growth, yield and quality of products. There was a positive response of plants to fertilizer K2SO4 in the period of planting. Correlation and regression analysis of yield and quality of tubers revealed a high degree of reliability of the results of experience. The contents of nitrates and heavy metals in tubers was below the permissible values.

Pengaruh Jenis Co-Solvent terhadap Rendemen dan Mutu Biodiesel secara Transesterifikasi In Situ Menggunakan Radiasi Gelombang Mikro

2016 ◽  
Vol 10 (2) ◽  
pp. 119-126
Author(s):  
Mahlinda Mahlinda ◽  
Fitriana Djafar
Keyword(s):  
Catalyst Concentration ◽  
Maximum Yield ◽  
Acid Value ◽  
Experimental Result ◽  
In Situ Transesterification ◽  
Yield And Quality ◽  
Solvent Type ◽  
Biodiesel Quality

The main purpose of this research was to observer effect co-solvent type (n-Hexane, chloroform and without co-solvent)  toward yield and quality of biodiesel via in situ transesterification process using microwave irradiation. The process was studied at microwave power 450 watt, reaction time 4 minutes, methanol to seed ratio 25:1 and catalyst concentration 5%. The physicochemical parameters of the biodiesel produced such as viscosity, density and acid value were analysed and compared with the SNI 7182-2012 standard. The experimental result showed the maximum yield biodiesel 78,32% obtained by using co-solvent chloroform.Test result of physicochemical properties (viscosity, density and acid value) of biodiesel products using co solvent n-Hexane, chloroform and without co solvent showed that these products conform to the SNI 7182-2012 standars. The type of co-solvent only affectedon biodiesel yield dan not affected on biodiesel quality (viscosity, density and acid value).  ABSTRAKTujuan penelitian ini adalah untuk mempelajari pengaruh jenis co-solvent (n-Hexane, chloroform dan tanpa co-solvent) terhadap rendemen dan mutu biodiesel secara trasesterifikasi in situ menggunakan radiasi gelombang mikro. Proses dilakukan pada daya gelombang mikro 450 watt, waktu reaksi 4 menit, perbandingan berat metanol terhadap bahan baku 25:1 dan jumlah katalis 5%. Parameter fisiko kimia dari produk biodiesel seperti viskositas, densitas dan angka asam di analisa dan dibandingkan dengan standar SNI 7182-2012 tentang biodiesel. Hasil penelitian menunjukkan rendemen maksimum biodiesel sebesar 78,32% diperoleh dengan menggunakan co-solvent chloroform. Hasil pengujian  karakteristik fisiko kimia (viskositas, densitas dan angka asam) dari produk biodiesel menggunakan co-solvent n-Hexane, chloroform dan tanpa co-solvent menunjukkan bahwa semua parameter ini masih memenuhi standar SNI 1782-2012 tentang biodiesel. Jenis co-solvent hanya berpengaruh pada rendemen biodiesel dan tidak berpengaruh terhadap mutu biodiesel (viskositas, densitas dan bilangan asam).Kata kunci: co-solvent, in situ transesterifikasi, microwave, rendemen, mutu   

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