Effects of Annurca Apple (Malus pumila cv Annurca) Polyphenols on Breast Cancer Cells

2019 ◽  
Vol 15 (7) ◽  
pp. 745-751 ◽  
Author(s):  
Stefania D'Angelo ◽  
Elisa Martino ◽  
Giovanna Cacciapuoti
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Morphological Changes ◽  
Molecular Data ◽  
Nuclear Condensation ◽  
A Cell ◽  
Morphological And Molecular Data ◽  
Inhibitory Agents ◽  
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Background: Dietary micronutrients have been proposed as effective inhibitory agents for cancer initiation, progression, and incidence. Among them, polyphenols, present in different foods and beverages, have retained attention in recent years. Apples are among the most consumed fruits worldwide, and several studies suggest that apple polyphenols could play a role in the prevention of degenerative diseases. Aims and Objectives: The present study aimed at evaluating the effects of Annurca flesh polyphenols extract (AFPE) effects of proliferation on MCF-7 cells. Methods: The data indicated that apple polyphenolic compounds had a significant antiproliferative action on MCF-7 cells and 500μM EqC AFPE induced a cell cycle arrest at G2/M. AFPE was also capable of inducing morphological changes as evidenced by nuclear condensation. Results: The cellular, morphological, and molecular data unequivocally suggested that induction of cellular apoptosis was mainly responsible for the previously observed antiproliferation-induced AFPE on MCF-7 cells. Conclusion: Taken together, AFPE that acts at a low micromolar range against breast cancer cells may be considered as a promising candidate for anticancer therapy.

Effects of the Gold Nanoparticles (AuNPs) on the Proliferation and Morphological Characteristics of Human Breast Cancer Cells (MCF-7) in Culture

2017 ◽  
Vol 268 ◽  
pp. 254-258 ◽  
Author(s):  
Nur Shafawati Rosli ◽  
Azhar Abdul Rahman ◽  
Azlan Abdul Aziz ◽  
Shaharum Shamsuddin ◽  
Nurul Sabihah Zakaria
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Culture Media ◽  
Morphological Changes ◽  
Morphological Characteristics ◽  
Morphological Observation ◽  
Duration Of Treatment ◽  
Significant Difference ◽  
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Ultrastructural characteristic and morphological changes of untreated and treated breast cancer MCF-7 cells were observed by energy-filtered transmission electron microscope (EFTEM). Morphological observation of MCF-7 after being treated with 13 nm, 50 nm, and 70 nm AuNPs, were looking unhealthy and dying out of the populace, the observed cells were more reduced and dying as treatment with 50 nm and 70 nm AuNPs. Cells detachment, clumping, shrunken, and dispersed cells in the culture medium and floating cells were also observed. The observed morphological changes increase in 50 nm and 70 nm AuNPs than in 13 nm AuNPs, which is less toxic to MCF-7 cells. The presented morphological analysis has established that 13 nm AuNPs showed less toxic to MCF-7 breast cancer cells. Whereas, control cells of MCF-7 were treated with only complete culture media, despite the duration of treatment, whereby the cells maintained most of their morphological features and observed to have a typical morphology of healthy cells that are well attached to the surface. These results indicate that AuNPs were clustered in the cells and there was no significant difference between images of different sizes of AuNPs observed in the cells, because the AuNPs always clustered together inside the cells.

scholarly journals Development of Functionalized silver Nanoparticles from Allamanda neriifolia Hook and their In Vitro Cytotoxic Effect on MCF-7 Cells

YMER Digital ◽  
2021 ◽  
Vol 20 (12) ◽  
pp. 385-396
Author(s):  
Sumathi R ◽  
◽  
Sivagamasundari K ◽  
Keyword(s):  
Breast Cancer ◽  
Silver Nanoparticles ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Morphological Changes ◽  
Total Phenolic ◽  
Biosynthesized Agnps ◽  
Average Size ◽  
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The present work demonstrates the influence of plant extract composition (antioxidant and total phenolic content) on the size and morphology of the produced AgNPs. In this study, silver nanoparticles (AgNPs) were synthesized using aqueous flower extract of Allamanda neriifolia plant. The biosynthetic procedure was rapid and simple and was easily monitored via colour changes and examined AgNPs (AN-AgNPs) by ultraviolet-visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM). The results obtained from various characterizations revealed that average size of synthesized AgNPs was 50 nm and in spherical structure. The anticancer potential of AN-AgNPs was investigated against human breast cancer cells (MCF-7). The cytotoxic response was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), and morphological changes by apoptosis. The biosynthesized AgNPs-induced cell death in MCF- 7 cells suggested the anticancer potential of AN-AgNPs. Therefore, they may be used to treat the breast cancer cells.

scholarly journals AWP1 Restrains the Aggressive Behavior of Breast Cancer Cells Induced by TNF-α

2021 ◽  
Vol 11 ◽  
Author(s):  
Eun-Young Kim ◽  
Ji-Eun Kim ◽  
Bongkun Choi ◽  
Jiyeon Kweon ◽  
Si-On Park ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Epithelial Mesenchymal Transition ◽  
Morphological Changes ◽  
Mesenchymal Cell ◽  
Loss Of Function ◽  
Mesenchymal Transition ◽  
Tnf Α ◽  
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TNF-α plays a crucial role in cancer initiation and progression by enhancing cancer cell proliferation, survival, and migration. Even though the known functional role of AWP1 (zinc finger AN1 type-6, ZFAND6) is as a key mediator of TNF-α signaling, its potential role in the TNF-α-dependent responses of cancer cells remains unclear. In our current study, we found that an AWP1 knockdown using short hairpin RNAs increases the migratory potential of non-aggressive MCF-7 breast cancer cells with no significant alteration of their proliferation in response to TNF-α. A CRISPR/Cas9-mediated AWP1 knockout in MCF-7 cells led to mesenchymal cell type morphological changes and an accelerated motility. TNF-α administration further increased this migratory capacity of these AWP1-depleted cells through the activation of NF-κB accompanied by increased epithelial-mesenchymal transition-related gene expression. In particular, an AWP1 depletion augmented the expression of Nox1, reactive oxygen species (ROS) generating enzymes, and ROS levels and subsequently promoted the migratory potential of MCF-7 cells mediated by TNF-α. These TNF-α-mediated increases in the chemotactic migration of AWP1 knockout cells were completely abrogated by an NF-κB inhibitor and a ROS scavenger. Our results suggest that a loss-of-function of AWP1 alters the TNF-α response of non-aggressive breast cancer cells by potentiating ROS-dependent NF-κB activation.

Mechanism research on the lupeol treatment on MCF-7 breast cancer cells based on cell metabonomics

2014 ◽  
Vol 32 (3) ◽  
pp. 278
Author(s):  
Dongdong SHI ◽  
Yuanyuan KUANG ◽  
Guiming WANG ◽  
Zhangxiao PENG ◽  
Yan WANG ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Mechanism Research ◽  
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