FoodSimplex as a Mean to Improve Portuguese Restaurants’ Goods Manufacturing Practices - Audit and Microbial Assessment

2020 ◽  
Vol 16 (9) ◽  
pp. 1449-1458
Author(s):  
Ana L. Baltazar ◽  
Ana Ferreira ◽  
Lara Manyes ◽  
Jordi Mañes
Keyword(s):  
Listeria Monocytogenes ◽  
Food Safety ◽  
Control Point ◽  
Food Samples ◽  
P Value ◽  
Salmonella Spp ◽  
E Coli ◽  
Critical Control Point ◽  
Audit Data ◽  
Coagulase Positive Staphylococci

Background: The consumption of meals at resturants has increased mass catering establishments. Such food services may present several risk factors for foodborne diseases, which have a substantial negative impact on public health. The smallest business with no technical or financial support struggles to comply with food safety European Regulation. The aim of this four years study was to assess Good Manufacture Practices (GMP) in Portuguese restaurants applying a designed food safety methodology, FoodSimplex, through audit data to determine good manufacture practices, and microbiological sampling of the meals before and after its implementation, by food groups according to their risk. Methods: FoodSimplex is a combination of four stages methodology, based on Hazards Analysis and Critical Control Point (HACCP), combined with technical-functional and food safety audits, with pretested checklist, training and microbial analysis (Mesophylic bacteria, Listeria monocytogenes, E. coli, Coagulase-positive staphylococci and Salmonella spp) of the meals served. Results: Results of the food safety audit for GMP parameters showed a general improvement with 80% compliance with the checklist subitems. The ready-to-eat food samples presented, regarding the total mesophilic aerobes count, a statistical significant change to acceptable and satisfactory condition (p-value= 0.029). 100% compliance, with acceptable and satisfactory results was observed for Listeria monocytogenes (p-value= 0.180) and for E. coli, coagulase-positive Staphylococci and Salmonella spp., all the food samples presented satisfactory results in the study (100%). Conclusion: At the end of the investigation period, a decrease in bacterial populations in food samples was observed with an improvement of the GMP audit data which indicated that the FoodSimplex methodology improved the food safety status of these establishments.

scholarly journals Evaluation of a Steam Pasteurization Process in a Commercial Beef Processing Facility

1997 ◽  
Vol 60 (5) ◽  
pp. 485-492 ◽  
Author(s):  
ABBEY L. NUTSCH ◽  
RANDALL K. PHEBUS ◽  
M. JAMES RIEMANN ◽  
DAVID E. SCHAFER ◽  
J. E. BOYER ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Control Point ◽  
Meat Products ◽  
Steam Treatment ◽  
Salmonella Spp ◽  
Bacterial Populations ◽  
E Coli ◽  
Critical Control Point ◽  
Beef Processing ◽  
Steam Pasteurization

The effectiveness of a steam pasteurization process for reducing naturally occurring bacterial populations on freshly slaughtered beef sides was evaluated in a large commercial facility. Over a period of 10 days, 140 randomly chosen beef sides were microbiologically analyzed. Each side was sampled immediately before, immediately after, and 24 h after steam pasteurization treatment. Total aerobic bacteria (APC), Escherichia coli (generic), coliform, and Enterobacteriaceae populations were enumerated. The process significantly (P ≤ 0.01) reduced mean APCs from 2.19 log CFU/cm2 before treatment to 0.84 log CFU/cm2 immediately after and 0.94 log CFU/cm2 24 h after treatment. Before pasteurization (8 s steam exposure), 16.4% of carcasses were positive for generic E. coli (level of 0.60 to 1.53 log CFU/cm2), 37.9% were positive for coliforms (level of 0.60 to 2.26 log CFU/cm2), and 46.4% were positive for Enterobacteriaceae (level of 0.60 to 2.25 log CFU/cm2). After pasteurization, 0% of carcasses were positive for E. coli, 1.4% were positive for coliforms (level of 0.60 to 1.53 log CFU/cm2), and 2.9% were positive for Enterobacteriaceae (level of 0.60 to 1.99 log CFU/cm2). Of the 140 carcasses evaluated, one carcass was positive for Salmonella spp. before treatment (0.7% incidence rate); all carcasses were negative after steam treatment. This study indicates that steam pasteurization is very effective in a commercial setting for reducing overall bacterial populations on freshly slaughtered beef carcasses. The system may effectively serve as an important critical control point for HACCP systems at the slaughter phase of beef processing. In conjunction with other antimicrobial interventions (mandated by USDA to achieve zero tolerance standards for visible contamination) and good manufacturing practices, this process can play an important role in reducing the risk of pathogenic bacteria in raw meat and meat products.

scholarly journals Hygienic-health quality and microbiological hazard of clandestine Minas Frescal cheese commercialized in north Tocantins, Brazil

2021 ◽  
Vol 42 (2) ◽  
pp. 679-694
Author(s):  
Monike da Silva Oliveira ◽  
◽  
Isac Gabriel Cunha dos Santos ◽  
Bianca Pereira Dias ◽  
Cristiane Alves Nascimento ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Food Product ◽  
Economic Loss ◽  
Daily Basis ◽  
Salmonella Spp ◽  
High Count ◽  
E Coli ◽  
High Concentrations ◽  
Sanitary Conditions ◽  
Coagulase Positive Staphylococci

Cheese is a popular product that integrates the diet of the majority of the population, almost on a daily basis. It is rich in nutrients and, therefore, also an excellent substrate for the multiplication of microorganisms, including pathogens. The microbiological contamination of these products is highly relevant to the industry, resulting in an economic loss, and to public health, due to the risk of causing foodborne diseases. The aim of this study was to evaluate the hygienic-sanitary quality and the presence of bacterial pathogens in the clandestine Minas Frescal cheeses sold in the street open markets of Araguaína, TO, Brazil. Twenty-one samples were collected to evaluate the presence of total (TC) and thermotolerant (TTC) coliforms, Escherichia coli, and the pathotypes enteropathogenic E. coli (EPEC), and shiga toxin-producing E. coli (STEC) and enterohemorrhagic E. coli (EHEC), coagulase-positive staphylococci (CPS), Salmonella spp., and Listeria monocytogenes. The results showed that 100% of the cheese samples were in disagreement with the maximum standards of TC and TTC defined in the Brazilian legislation that regulates food quality control. In addition, 85.71% of the samples had values above the standard Brazilian maximum limit. In the study of diarrheagenic E. coli, 52.38%, 66.6%, and 4.76% of the samples were positive for EPEC, STEC, and EHEC, respectively, indicating fecal origin contamination of the samples and a potential consumer risk. No Salmonella spp. or Listeria monocytogenes were detected in these cheese samples. The high count of total and thermotolerant coliforms found in the samples demonstrates unsatisfactory sanitary conditions in the production, storage, and/or commercialization of this food product. The presence of EPEC, STEC, EHEC, and coagulase-positive staphylococci at high concentrations shows the health risk of the imminent consumption of Minas Frescal cheese.

A Review of the Incidence and Transmission of Listeria monocytogenes in Ready-to-Eat Products in Retail and Food Service Environments

2007 ◽  
Vol 70 (9) ◽  
pp. 2172-2198 ◽  
Author(s):  
ALEXANDRA LIANOU ◽  
JOHN N. SOFOS
Keyword(s):  
Listeria Monocytogenes ◽  
Food Safety ◽  
Control Point ◽  
Food Service ◽  
Service Operations ◽  
Control Measures ◽  
Effective Control ◽  
Successful Implementation ◽  
Food Handlers ◽  
Critical Control Point

Contamination of ready-to-eat products with Listeria monocytogenes may occur at several stages before consumption. Accessibility to the public and relatively limited control interventions at retail and food service establishments (compared with the processing sector of the food industry) and the lack of a specific regulatory framework increase the likelihood of introduction of this pathogen into some foods in these establishments. This review is a compilation of available information on the incidence and transmission of L. monocytogenes through ready-to-eat products at the retail and food service level. The potential transmission of L. monocytogenes within retail and food service operations has been indicated in epidemiological investigations and by survey data. Potential sources of the organism in these operations include the environment, food handlers, and incoming raw ingredients or processed products that have become contaminated after the lethality treatment at the manufacturing facility. L. monocytogenes may be present at retail and food service establishments in various ready-to-eat products, both prepackaged and those packaged in the store, and occasionally at high concentrations. This issue dictates the need for development and application of effective control measures, and potential control approaches are discussed here. Good manufacturing practices, appropriate cleaning, sanitation and hygiene programs, and temperature control required for prevention or inhibition of growth of the pathogen to high levels are critical for control of L. monocytogenes in the retail and food service sector. A comprehensive food safety system designed to be functional in retail and food service operations and based on the philosophy of hazard analysis and critical control point systems and a series of sound prerequisite programs can provide effective control of L. monocytogenes in these environments. However, competent delivery of food safety education and training to retail and food service managers and food handlers must be in place for successful implementation of such a system.

Food Safety Objectives for Listeria monocytogenes in Spanish Food Sampled in Cafeterias and Restaurants

2011 ◽  
Vol 74 (9) ◽  
pp. 1569-1573 ◽  
Author(s):  
E. DOMÉNECH ◽  
J. A. AMORÓS ◽  
I. ESCRICHE
Keyword(s):  
Listeria Monocytogenes ◽  
Food Safety ◽  
Safety Management ◽  
Hazard Analysis ◽  
Control Point ◽  
Consumer Health ◽  
Critical Control Point ◽  
Good Manufacturing Practices ◽  
Microbiological Criteria ◽  
Insight Into

To gain more insight into the context of food safety management by public administrations, food safety objectives must be studied. The Valencian administration quantified the prevalence of Listeria monocytogenes in cafeterias and restaurants in this region of Spain between 2002 and 2010. The results obtained from this survey are presented here for 2,262 samples of fish, salad, egg, cold meat, and mayonnaise dishes. Microbiological criteria defined for L. monocytogenes were used to differentiate acceptable and unacceptable samples; more than 99.9% of the samples were acceptable. These findings indicate that established food safety objectives are achievable, consumer health at the time of consumption can be safeguarded, and food safety management systems such as hazard analysis critical control point plans or good manufacturing practices implemented in food establishments are effective. Monitoring of foods and food safety is an important task that must continue to reduce the current L. monocytogenes prevalence of 0.1% in restaurant or cafeteria dishes, which could adversely affect consumer health.

scholarly journals Contribution of natural milk culture to microbiota, safety and hygiene of raw milk cheese produced in alpine malga

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Rosaria Lucchini ◽  
Barbara Cardazzo ◽  
Lisa Carraro ◽  
Michele Negrinotti ◽  
Stefania Balzan ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Safety Evaluation ◽  
Raw Milk ◽  
Starter Cultures ◽  
Salmonella Spp ◽  
E Coli ◽  
First Case ◽  
Bacterial Biodiversity ◽  
Natural Milk ◽  
Coagulase Positive Staphylococci

Processing of alpine milk in malga farms is carried out under conditions that can favor contamination by coliforms, coagulase-positive staphylococci, or pathogens such as Listeria monocytogenes. With the aim to improve the hygienic characteristics and safety of cheese produced in four malga farms the use of lyophilized Natural Milk Culture prepared with selected strains was tested. Two cheesemaking tests were carried out in the same day always starting from the same milk: in the first case following the malga recipe that uses either Natural Whey Culture or without the addition of a starter, in the second one using a Natural Milk Culture. Cheesemaking were carried out in four malga farms located in the west area of Trentino region within the same week. For hygienic and safety evaluation, aerobic colony count, coagulase-positive staphylococci, Escherichia coli, staphylococcal toxins, Listeria monocytogenes , and Salmonella spp, pH and aw were determined in raw milk from evening and morning milking, curd in vat, curd after extraction and two months-ripened cheese. Pathogens or toxins, high values of coagulase- positive staphylococci and E. coli were not found in cheese samples. However, in the curd coagulase-positive staphylococci reached values almost of 5 Log CFU/g in the two malga without starter cultures. The use of Natural Milk Culture reduced E. coli counts. In addition, DNA was extracted from cheese samples and from Natural Milk Culture and the composition of the microbial community determined by Next Generation Sequencing method. The determination of cheese microbial communities demonstrated that the use of Natural Milk Culture exerted different effects in the different malga, in any case preserving bacterial biodiversity.

scholarly journals Effect of Structural Condition of Milk Processing Facilities and Food Safety Systems on Escherichia coli and Coliforms Presence in Cultured Buttermilk

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Annah S. Paraffin ◽  
Titus J. Zindove ◽  
Michael Chimonyo
Keyword(s):  
Food Safety ◽  
Large Scale ◽  
Hazard Analysis ◽  
Control Point ◽  
Structural Condition ◽  
Small Scale ◽  
E Coli ◽  
Milk Processing ◽  
Critical Control Point ◽  
Safety Systems

The study investigated the effect of structural conditions of milk processing facilities and food safety systems on E. coli and coliform presence in buttermilk. Milk records collected by Dairy Services Zimbabwe (DSZ) from large-scale dairy milk processors (n=12) and small-scale farms (n=15) were analysed. Binomial logistic regression was used to estimate the likelihood of E. coli or coliforms being present in cultured buttermilk as a function of the hygiene level and structural adequacy of the processors. The likelihood of having E. coli and coliforms in cultured milk from processors with poor sanitary premises was two times higher than that from processors with good sanitary premises (P<0.05). Milk processors that used unfiltered water were 1.77 times more likely to produce cultured buttermilk contaminated with E. coli (P<0.05). Processors without food safety systems like hazard analysis critical control point (HACCP) systems were more than twice likely to produce cultured buttermilk contaminated by E. coli and coliforms (P<0.05). Poor structural condition of roofs, windows, insect-proof screens, and drainage in small- and large-scale processing facilities results in production of cultured buttermilk that is contaminated by E. coli and coliforms.

An Overview of Microbial Food Safety Programs in Beef, Pork, and Poultry from Farm to Processing in Canada

2007 ◽  
Vol 70 (5) ◽  
pp. 1286-1294 ◽  
Author(s):  
ANDRIJANA RAJIĆ ◽  
LISA A. WADDELL ◽  
JAN M. SARGEANT ◽  
SUSAN READ ◽  
JEFF FARBER ◽  
...  
Keyword(s):  
Food Safety ◽  
Hazard Analysis ◽  
Control Point ◽  
Shared Responsibility ◽  
Ground Meat ◽  
E Coli ◽  
Critical Control Point ◽  
Verification Tools ◽  
Canadian Food Inspection Agency ◽  
Food Commodities

Canada's vision for the agri-food industry in the 21st century is the establishment of a national food safety system employing hazard analysis and critical control point (HACCP) principles and microbiological verification tools, with traceability throughout the gate-to-plate continuum. Voluntary on-farm food safety (OFFS) programs, based in part on HACCP principles, provide producers with guidelines for good production practices focused on general hygiene and biosecurity. OFFS programs in beef cattle, swine, and poultry are currently being evaluated through a national recognition program of the Canadian Food Inspection Agency. Mandatory HACCP programs in federal meat facilities include microbial testing for generic Escherichia coli to verify effectiveness of the processor's dressing procedure, specific testing of ground meat for E. coli O157:H7, with zero tolerance for this organism in the tested lot, and Salmonella testing of raw products. Health Canada's policy on Listeria monocytogenes divides ready-to-eat products into three risk categories, with products previously implicated as the source of an outbreak receiving the highest priority for inspection and compliance. A national mandatory identification program to track livestock from the herd of origin to carcass inspection has been established. Can-Trace, a data standard for all food commodities, has been designed to facilitate tracking foods from the point of origin to the consumer. Although much work has already been done, a coherent national food safety strategy and concerted efforts by all stakeholders are needed to realize this vision. Cooperation of many government agencies with shared responsibility for food safety and public health will be essential.

Salmonella spp. and Escherichia coli Biotype I on Swine Carcasses Processed under the Hazard Analysis and Critical Control Point–Based Inspection Models Project

2001 ◽  
Vol 64 (9) ◽  
pp. 1305-1308 ◽  
Author(s):  
MARK L. TAMPLIN ◽  
INGRID FEDER ◽  
SAMUEL A. PALUMBO ◽  
ALAN OSER ◽  
LISA YODER ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hazard Analysis ◽  
Control Point ◽  
Inspection System ◽  
Processing Plant ◽  
Salmonella Spp ◽  
E Coli ◽  
Critical Control Point ◽  
The Mean ◽  
Processing Steps

The present study examined the prevalence of Salmonella spp. and the prevalence and quantity of generic (biotype I) Escherichia coli on carcasses or in pig feces at a pork processing plant operating under the hazard analysis and critical control point–based inspection models project (HIMP) program. The surfaces of carcasses were sponged on 10 separate days over a 30-day period at two processing steps: (i) immediately following exsanguination (100 carcasses), and (ii) after the carcasses were washed, eviscerated, and chilled overnight (122 carcasses). Feces were also collected from 60 of the 100 sponged, postexsanguinated pigs. Salmonella spp. were detected on 73.0% of the 100 postexsanguinated pigs, in 33.3% of the 60 fecal samples, and on 0.7% of the 122 chilled carcasses. E. coli was found on 100.0% of the postexsanguinated pigs and on 30.1% of chilled carcasses tested. The mean concentration of E. coli on carcasses was 1,700 CFU/cm2 immediately after the exsanguination step and 1.1 CFU/cm2 at the chilled carcass stage. Previous studies at this processing plant showed that the pre-HIMP baseline level of Salmonella spp. on the chilled carcasses was 0.8%, indicating that the present HIMP inspection system produced an equivalent level of bacteriological performance.

scholarly journals Assessment of Hazard Analysis Critical Control Point (HACCP) of Fast Food (Momo) from Restaurants of Kathmandu City

1970 ◽  
Vol 9 ◽  
pp. 49-56
Author(s):  
Poonam Thapa ◽  
Anjana Singh ◽  
Tika Bahadur Karki
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Fast Food ◽  
Proper Time ◽  
Hazard Analysis ◽  
Control Point ◽  
Salmonella Spp ◽  
Coliform Count ◽  
Critical Control Point ◽  
Mold Count

Hazard analysis critical control point (HACCP) module was prepared for one of the most popular fast food momo (chicken momo and buff momo). For this, hazard analysis was conducted in eight different restaurants of Katmandu city by observing all the steps of preparation, monitoring time-temperature throughout the preparation process and collecting samples of different stages of these food. The samples were assessed for total aerobic mesophilic count (TAMC), total coliform count, total Staphylococcus aureus count, total yeast and mold count, detection of Salmonella spp. and Escherichia coli. During preparation of chicken momo, the highest TAMC, yeast and mold count, coliform and S. aureus count were found to be 2.8 × 106cfu/g, 2.1 × 103cfu/g, 1.92 × 105cfu/g and 3.4 × 103cfu/g respectively. While preparation of buff momo, the highest TAMC, yeast and mold count, coliform count and S. aureus count were found to be 2.82 × 106cfu/g, 1.9 × 103cfu/g, 2.1 × 105cfu/g and 2.8 × 103cfu/g respectively. These values and near to these values too were obtained from the samples of pickles, spices, raw momo, mixture of minced meat with spices and raw meat. The organisms originally present in the raw materials were subsequently transmitted to all the preparatory stages but was not observed after steaming and hence the final steamed product of both kinds of momo were free from microorganisms. Thus from the above findings, it was concluded that steaming was the main critical control point (CCP), which if done for proper time and temperature, can eliminate all the contaminating organisms. Key words: coliform count, critical control point, hazard analysis, Staphylococcus aureus, Escherichia coli, Salmonella spp DOI: 10.3126/njst.v9i0.3164 Nepal Journal of Science and Technology 9 (2008) 49-56

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