Optimization and Kinetic Modeling of Rosmarinic Acid Extraction from Orthosiphon stamineus

2015 ◽  
Vol 10 (4) ◽  
pp. 271-285 ◽  
Author(s):  
Cher H. Lau ◽  
Lee S. Chua ◽  
Chew T. Lee ◽  
Ramlan Aziz
Keyword(s):  
Kinetic Modeling ◽  
Rosmarinic Acid ◽  
Acid Extraction ◽  
Orthosiphon Stamineus

scholarly journals Solvation Free Energy Simulation for Rosmarinic Acid Extraction from Orthosiphon stamineus

2019 ◽  
Vol 2 (3) ◽  
pp. 64 ◽  
Author(s):  
Cher Haan Lau ◽  
Lee Suan Chua
Keyword(s):  
Free Energy ◽  
Crude Extract ◽  
Rosmarinic Acid ◽  
Radical Scavenging ◽  
Value Added ◽  
Solvent System ◽  
Solvation Free Energy ◽  
Energy Simulation ◽  
Acid Extraction ◽  
Orthosiphon Stamineus

This study was aimed to extract rosmarinic acid from Orthosiphon stamineus Benth. (Lamiaceae) in high yield. The mixture of chloroform–ethyl acetate (70:30) was chosen as the solvent system because rosmarinic acid gave the lowest solvation free energy in that solvent system based on the computational solubility prediction. The crude extract of the plant was fractionated by C18 reversed phase absorbent to recover rosmarinic acid. The content of rosmarinic acid was increased from 4.0% w/w to 6.7% w/w after fractionation. The radical scavenging activity of rosmarinic acid rich fraction (IC50 = 38.3 μg/mL) was higher than the crude extract (IC50 = 58.85 μg/mL) based on the DPPH assay. Several phytochemicals were also identified based on the detection of fragment ions of target compounds. Fractions 1 to 3 could be combined to be a rosmarinic acid rich fraction. Simultaneously, the combination of fractions 4 to 6 could obtain a plant fraction rich in rosmarinic acid, sinensetin and eupatorin, whereas fractions 7 to 9 could be combined as a sinensetin rich fraction. The preparation of known phytochemical profile of O. stamineus fraction is highly required for value added product formulation and pharmacological studies, particularly for anti-diabetes and kidney related diseases which had previously been reported attributed to this herbal plant. This is the first study using solvation free energy to predict the suitable solvent system for rosmarinic acid extraction from highly complex herbal sample using the technology of solid phase extraction. The use of solvation free energy simulation is convenient and reliable before wet experiments for time and cost saving.

scholarly journals Effect of Drying Temperature on Rosmarinic Acid and Sinensetin Concentration in Orthosiphon stamineus Herbal Leaves

2018 ◽  
Vol 318 ◽  
pp. 012074 ◽  
Author(s):  
Sriyana Abdullah ◽  
Abdul Razak Shaari ◽  
Ibni Hajar Rukunudin ◽  
Muhammad Syarhabil Ahmad
Keyword(s):  
Rosmarinic Acid ◽  
Drying Temperature ◽  
Orthosiphon Stamineus

Orthosiphon stamineus and rosmarinic acid reduce heat stress in laying hens

2020 ◽  
Vol 240 ◽  
pp. 104124
Author(s):  
Xuan Cai ◽  
Lu Zhang ◽  
Xujie Chen ◽  
Hongzhi Zhang ◽  
Huiqin Xue ◽  
...  
Keyword(s):  
Heat Stress ◽  
Rosmarinic Acid ◽  
Laying Hens ◽  
Orthosiphon Stamineus

scholarly journals HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHIC DETERMINATION OF CAFFEIC ACID AND ROSMARINIC ACID FROM THE LEAVES OF Orthosiphon stamineus

2010 ◽  
Vol 9 (1) ◽  
pp. 137-141
Author(s):  
M. Amzad Hossain ◽  
Zhari Ismail
Keyword(s):  
Thin Layer ◽  
Caffeic Acid ◽  
Rosmarinic Acid ◽  
High Performance ◽  
Chromatographic Determination ◽  
Hplc Method ◽  
Caffeic Acid Derivatives ◽  
Orthosiphon Stamineus ◽  
Pharmaceutical Properties

This paper presents the studies performed on extraction of Orthosiphon stamineus, Benth by using different solvent for the identification and quantification of the caffeic acid derivatives such as caffeic acid  and rosmarinic acid which confers to the leaves of this plant with remarkable pharmaceutical properties. High performance thin-layer chromatographic (HPTLC) allows the identification and the quantification of more than 20 samples in the same chromatographic run. The analysis of the samples requires 15-30 min compared with more than 2 h using a typical HPLC method. Using the techniques of the HPTLC and the UV-VIS spectra we have found that the extraction of this herb plant contain, the caffeic acid and rosmarinic acid ranging between 0.029% up to 0.506% and up to 0.24% to 2.24% respectively.     Keywords: Caffice acid derivatives, quantification, Malaysian Orthosiphon stamineus, HPTLC

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