Characterization of Gemcitabine Loaded Polyhydroxybutyrate Coated Magnetic Nanoparticles for Targeted Drug Delivery

2020 ◽  
Vol 20 (10) ◽  
pp. 1233-1240
Author(s):  
Maryam Parsian ◽  
Pelin Mutlu ◽  
Serap Yalcin ◽  
Ufuk Gunduz
Keyword(s):  
Breast Cancer ◽  
Magnetic Field ◽  
Drug Delivery ◽  
Magnetic Nanoparticles ◽  
Targeted Drug Delivery ◽  
Targeted Drug ◽  
Drug Free ◽  
The Magnetic Field ◽  
Mcf 7

Background: Targeted drug delivery is one of the recent hot topics in cancer therapy. Because of having a targeting potential under the magnetic field and a suitable surface for the attachment of different therapeutic moieties, magnetic nanoparticles are widely studied for their applications in medicine. Objective: Gemcitabine loaded polyhydroxybutyrate coated magnetic nanoparticles (Gem-PHB-MNPs) were synthesized and characterized for the treatment of breast cancer by the targeted drug delivery method. Methods: The characterization of nanoparticles was confirmed by FTIR, XPS, TEM, and spectrophotometric analyses. The cytotoxicities of drug-free nanoparticles and Gemcitabine loaded nanoparticles were determined with cell proliferation assay using SKBR-3 and MCF-7 breast cancer cell lines. Result: The release of Gemcitabine from PHB-MNPs indicated a pH-dependent pattern, which is a desirable release characteristic, since the pH of the tumor microenvironment and endosomal structures are acidic, while bloodstream and healthy-tissues are neutral. Drug-free PHB-MNPs were not cytotoxic to the SKBR-3 and MCF- 7 cells, whereas the Gemcitabine loaded PHB-MNPs was about two-fold as cytotoxic with respect to free Gemcitabine. In vitro targeting ability of PHB-MNPs was shown under the magnetic field. Conclusion: Considering these facts, we may suggest that these nanoparticles can be a promising candidate for the development of a novel targeted drug delivery system for breast cancer.

scholarly journals Nanoformulation Design Including MamC-Mediated Biomimetic Nanoparticles Allows the Simultaneous Application of Targeted Drug Delivery and Magnetic Hyperthermia

Polymers ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 1832 ◽  
Author(s):  
Ylenia Jabalera ◽  
Francesca Oltolina ◽  
Ana Peigneux ◽  
Alberto Sola-Leyva ◽  
Maria P. Carrasco-Jiménez ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Drug Delivery ◽  
Magnetic Nanoparticles ◽  
Targeted Drug Delivery ◽  
Magnetic Hyperthermia ◽  
Simultaneous Application ◽  
Biomimetic Nanoparticles ◽  
Targeted Drug ◽  
Model Drug ◽  
Drug Nanocarriers

The design of novel nanomaterials that can be used as multifunctional platforms allowing the combination of therapies is gaining increased interest. Moreover, if this nanomaterial is intended for a targeted drug delivery, the use of several guidance methods to increase guidance efficiency is also crucial. Magnetic nanoparticles (MNPs) allow this combination of therapies and guidance strategies. In fact, MNPs can be used simultaneously as drug nanocarriers and magnetic hyperthermia agents and, moreover, they can be guided toward the target by an external magnetic field and by their functionalization with a specific probe. However, it is difficult to find a system based on MNPs that exhibits optimal conditions as a drug nanocarrier and as a magnetic hyperthermia agent. In this work, a novel nanoformulation is proposed to be used as a multifunctional platform that also allows dual complementary guidance. This nanoformulation is based on mixtures of inorganic magnetic nanoparticles (M) that have been shown to be optimal hyperthermia agents, and biomimetic magnetic nanoparticles (BM), that have been shown to be highly efficient drug nanocarriers. The presence of the magnetosome protein MamC at the surface of BM confers novel surface properties that allow for the efficient and stable functionalization of these nanoparticles without the need of further coating, with the release of the relevant molecule being pH-dependent, improved by magnetic hyperthermia. The BM are functionalized with Doxorubicin (DOXO) as a model drug and with an antibody that allows for dual guidance based on a magnetic field and on an antibody. The present study represents a proof of concept to optimize the nanoformulation composition in order to provide the best performance in terms of the magnetic hyperthermia agent and drug nanocarrier.

Preparation and Characterization of Fe3O4 Magnetic Nanoparticles Labeled with Technetium-99m Pertectnetate

2013 ◽  
Vol 459 ◽  
pp. 51-59 ◽  
Author(s):  
Chang Shu Tsai ◽  
Wei Chung Liu ◽  
Hong Yi Chen ◽  
Wei Chun Hsu
Keyword(s):  
Drug Delivery ◽  
Magnetic Nanoparticles ◽  
Targeted Drug Delivery ◽  
Magnetic Nanoparticle ◽  
Drug Carrier ◽  
Precipitation Method ◽  
Technetium 99M ◽  
Reducing Ability ◽  
Targeted Drug

In the aspect of biomedical diagnosis, magnetic nanoparticle can be used as drug carrier and MRI/ SPECT/ PET contrast agents. Magnetic fluid hyperthermia is one of the most important cancer therapies. Magnetic nanoparticles display their unique features as heating mediators for hyperthermia. In this study, Fe3O4magnetic nanoparticle was prepared by using chemical co-precipitation method. Tc-99m pertechnetate with Fe3O4magnetic nanoparticles is prepared by using magnet adsorption method. An attempt was also made to evaluate the application in the field of magnetic targeted drug delivery and radioactive targeted cancer treatment in the future. In this work, preparation and characterization of non-polymer and polymer (dextran) coated Fe3O4magnetic nanoparticles labeled with technetium-99m pertectnetate were evaluated and served as precursors study. The Tc-99m labeling efficiency of in-house Fe3O4magnetic nanoparticles (MNP) and commercial kit were ca.98.4 % and 85% (n=5), under the same conc. of 6mM, 0.1 ml of SnCl2·2H2O, respectively. The Tc-99m labeling efficiency of magnetic nanoparticles with its dextran-coated was ca. 58.2% (n=5) at the same conc. and volume of SnCl2·2H2O. The in-vitro stabilities of the 3 kinds of magnetite magnetic fluids were higher than 96.0% (n=5) during 2 hours. The reducing agent of SnCl2·2H2O plays a key role due to its reducing ability for Tc-99m pertechnetate. The optimal reaction time of SnCl2·2H2O with Tc-99m is better under 1 hour. In conclusion, the Fe3O4magnetic nanoparticle labeled with Tc-99m pertechnetate has shown good qualities for its labeling efficiency and stability. It may be feasible preliminary to utilize in the application of magnetic targeted drug delivery of bio-medicine.

Ultrafast synthesis of stabilized gold nanoparticles using aqueous fruit extract of Limonia acidissima L. and conjugated epirubicin: targeted drug delivery for treatment of breast cancer

RSC Advances ◽  
2016 ◽  
Vol 6 (32) ◽  
pp. 26874-26882 ◽  
Author(s):  
C. Senthil Kumar ◽  
Ayyavu Mahesh ◽  
M. Gover Antoniraj ◽  
S. Vaidevi ◽  
K. Ruckmani
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Folic Acid ◽  
Targeted Drug Delivery ◽  
Fruit Extract ◽  
Site Specific ◽  
Targeted Drug ◽  
Limonia Acidissima ◽  
Mcf 7

Green synthesis of AuNPs using extract ofLimonia acidissimaL. as a reducing agent. Epirubicin was successfully loaded on folic acid–AuNPs. This formulation revealed better site specific delivery of epirubicin to MCF-7 cells.

Trajectory Simulation of Magnetic Nanoparticles in the Blood Vessel for the Magnetic Targeted-Drug Delivery

2013 ◽  
Vol 753-755 ◽  
pp. 988-994 ◽  
Author(s):  
Jie Feng ◽  
Rui Lin Zhang ◽  
Ya Nan Qu ◽  
Ping Geng ◽  
Shou Liang Qi
Keyword(s):  
Magnetic Field ◽  
Drug Delivery ◽  
Magnetic Nanoparticles ◽  
Theoretical Model ◽  
Blood Vessel ◽  
Targeted Drug Delivery ◽  
Magnetic Circuit ◽  
Critical Parameters ◽  
Retention Ratio ◽  
Targeted Drug

Magnetic nanoparticles (MNPs) have been considered as potential therapeutic agent carrier for the magnetic targeted-drug delivery in the fight against cancer. Trajectories of MNPs in the blood vessel determine the capture and retention ratio, and the final effectiveness of the treatment. In the present study, a theoretical model of MNPs trajectory is deduced at first. Then two kinds of magnets are proposed, and their magnetic field distributions are calculated through the finite element method software of ANSYS. Using the model and magnetic field inputs, the MNPs trajectories are determined, and the influences of the MNP diameter (Rp), the blood flow velocity (vf) and magnetic field intensity (H) on the trajectories are clarified finally. It is found that the proposed method combining the theoretical model and numerical simulation is feasible. The closed magnetic circuit with concave-convex poles has better MNPs retention ratio than that of the open magnetic circuit because it has higher H and Grad (H). LargeRp, lowvf, and high H are good to capture the MNPs. Especiallyvfand H are critical parameters for the retention ratio of MNPs, and highvfand low H may let MNPs escape the magnetic field region.

scholarly journals New advances on Au–magnetic organic hybrid core–shells in MRI, CT imaging, and drug delivery

RSC Advances ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 6517-6525
Author(s):  
Fatemeh Mohajer ◽  
Ghodsi Mohammadi Ziarani ◽  
Alireza Badiei
Keyword(s):  
Magnetic Resonance Imaging ◽  
Drug Delivery ◽  
Magnetic Nanoparticles ◽  
Targeted Drug Delivery ◽  
Magnetic Storage ◽  
Resonance Imaging ◽  
Organic Hybrid ◽  
Storage Media ◽  
Targeted Drug ◽  
Magnetic Storage Media

Magnetic nanoparticles have been studied for scientific and technological applications such as magnetic storage media, contrast agents for magnetic resonance imaging, biolabelling, separation of biomolecules, and magnetic-targeted drug delivery.

scholarly journals Folic Acid-Terminated Poly(2-Diethyl Amino Ethyl Methacrylate) Brush-Gated Magnetic Mesoporous Nanoparticles as a Smart Drug Delivery System

Polymers ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 59
Author(s):  
Abeer M. Beagan ◽  
Ahlam A. Alghamdi ◽  
Shatha S. Lahmadi ◽  
Majed A. Halwani ◽  
Mohammed S. Almeataq ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Drug Resistance ◽  
Folic Acid ◽  
Drug Delivery System ◽  
Targeted Drug Delivery ◽  
Ph Responsive ◽  
Ethyl Methacrylate ◽  
Mesoporous Nanoparticles ◽  
Targeted Drug ◽  
Mcf 7

Currently, chemotherapy is an important method for the treatment of various cancers. Nevertheless, it has many limitations, such as poor tumour selectivity and multi-drug resistance. It is necessary to improve this treatment method by incorporating a targeted drug delivery system aimed to reduce side effects and drug resistance. The present work aims to develop pH-sensitive nanocarriers containing magnetic mesoporous silica nanoparticles (MMSNs) coated with pH-responsive polymers for tumour-targeted drug delivery via the folate receptor. 2-Diethyl amino ethyl methacrylate (DEAEMA) was successfully grafted on MMSNs via surface initiated ARGET atom transfer radical polymerization (ATRP), with an average particle size of 180 nm. The end groups of poly (2-(diethylamino)ethyl methacrylate) (PDEAEMA) brushes were converted to amines, followed by a covalent bond with folic acid (FA) as a targeting agent. FA conjugated to the nanoparticle surface was confirmed by X-ray photoelectron spectroscopy (XPS). pH-Responsive behavior of PDEAEMA brushes was investigated by Dynamic Light Scattering (DLS). The nanoparticles average diameters ranged from ca. 350 nm in basic media to ca. 650 in acidic solution. Multifunctional pH-sensitive magnetic mesoporous nanoparticles were loaded with an anti-cancer drug (Doxorubicin) to investigate their capacity and long-circulation time. In a cumulative release pattern, doxorubicin (DOX) release from nano-systems was ca. 20% when the particle exposed to acidic media, compared to ca. 5% in basic media. The nano-systems have excellent biocompatibility and are minimally toxic when exposed to MCF-7, and -MCF-7 ADR cells.

Identification of protein targets and the mechanism of the cytotoxic action of Ipomoea turpethum extract loaded nanoparticles against breast cancer cells

2019 ◽  
Vol 7 (39) ◽  
pp. 6048-6063 ◽  
Author(s):  
Mohd Mughees ◽  
Mohd Samim ◽  
Yadhu Sharma ◽  
Saima Wajid
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Cancer Cells ◽  
Targeted Drug Delivery ◽  
Breast Cancer Cells ◽  
Cytotoxic Action ◽  
Protein Targets ◽  
Targeted Drug

The shortcomings of the currently available anti-breast cancer agents compel the development of the safer targeted drug delivery for the treatment of breast cancer.

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