Characterization of antimicrobial compound produced from Hericium erinaceus combating Campylobacter jejuni NCTC11168

2020 ◽  
Vol 18 ◽  
Author(s):  
Basma Hamdy ◽  
Mohamed Yosri ◽  
Nermin Abed ◽  
Sayed Abel El- Kareem ◽  
Amal Ellithy ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Bioactive Compound ◽  
Antimicrobial Activities ◽  
Hericium Erinaceus ◽  
Severe Diarrhea ◽  
Antibacterial Screening ◽  
Enteric Infections ◽  
Fractionation Pattern ◽  
Layer Chromatography

Background: Campylobacter jejuni is the most common cause of enteric infections, particularly among children, resulting in severe diarrhea. Increasing drug resistance of this bacterium against standard antibiotics, favors investigations into additional anti-Campylobacter medications that are already used to overcome effects on enteric infections. Methods: Anti-bacterial activity using well diffusion assay of seventeen fungal extracts were tested against C. jejuni NCTC11168. The obtained results of antibacterial screening showed that different tested fungal isolates have different antimicrobial activities, where Hericium erinaceus extract was the highest activity against tested bacterium. Results: Fractionation pattern has been done by column chromatography. Furthermore, purity was estimated by thin layer chromatography (TLC). Minimal inhibitory concertation (MIC) for the purified compound was 7.81μg/ml. Cytotoxicity for the purified compound was evaluated to be 170μg/ml. 1HNMR, IR and GC-Mass were performed for illustration of suggested structure of bioactive compound purified from H. erinaceus. Conclusion: The data presented here suggested that H. erinaceus could potentially be used in modern applications aimed at the treatment or prevention of Campylobacter jejuni infection.

scholarly journals Antimicrobial Activity of Artemisinin and Precursor Derived fromIn VitroPlantlets ofArtemisia annuaL.

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Suganthi Appalasamy ◽  
Kiah Yann Lo ◽  
Song Jin Ch'ng ◽  
Ku Nornadia ◽  
Ahmad Sofiman Othman ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Artemisia Annua ◽  
Antimicrobial Property ◽  
Bioactive Compound ◽  
Antimicrobial Activities ◽  
Hot Climate ◽  
Inhibition Concentration ◽  
Brine Shrimps ◽  
Layer Chromatography

Artemisia annuaL., a medicinal herb, produces secondary metabolites with antimicrobial property. In Malaysia due to the tropical hot climate,A. annuacould not be planted for production of artemisinin, the main bioactive compound. In this study, the leaves of threein vitro A. annuaL. clones were, extracted and two bioactive compounds, artemisinin and a precursor, were isolated by thin layer chromatography. These compounds were found to be effective in inhibiting the growth of Gram-positive and Gram-negative bacteria but notCandida albicans. Their antimicrobial activity was similar to that of antibactericidal antibiotic streptomycin. They were found to inhibit the growth of the tested microbes at the minimum inhibition concentration of 0.09 mg/mL, and toxicity test using brine shrimp showed that even the low concentration of 0.09 mg/mL was very lethal towards the brine shrimps with 100% mortality rate. This study hence indicated thatin vitrocultured plantlets ofA. annuacan be used as the alternative method for production of artemisinin and its precursor with antimicrobial activities.

Isolation, Screening and Antimicrobial Activity of Aeromonas hydrophila from Spoiled Vegetables and Biochemical Analysis of Estuarine Microbial Sediments

2020 ◽  
Vol 9 (3) ◽  
pp. 192-197
Author(s):  
Kumar Swathi ◽  
Duraisamy Ramachandhiran ◽  
Raju Murali ◽  
Veerasamy Vinothkumar
Keyword(s):  
Antimicrobial Activity ◽  
Aeromonas Hydrophila ◽  
Biochemical Analysis ◽  
Bioactive Compound ◽  
Antimicrobial Activities ◽  
Gram Negative ◽  
Enzyme Screening ◽  
Pseudomonas Species ◽  
Layer Chromatography ◽  
Spore Forming Bacteria

Background: Aeromonas hydrophila is a heterotrophic, Gram-negative, rod-shaped, facultative anaerobic, non-spore forming bacteria that are autochthonous and widely dispersed in marine environments. The study aims at investigating the screening of Aeromonas hydrophila from spoiled vegetables and the sediment sample collected from three different estuaries located in the Bay of Bengal (Vedharanyam, Parangipettai and Pichavaram, Tamilnadu, India) for the presence of enzymes and antimicrobial activities. Objective: Isolation, enzyme screening, antimicrobial activity of Aeromonas hydrophila from spoiled vegetables and three different estuarine microbial sediment samples for the purpose of biochemical and enzymatic analysis. Methods: The bioactive compound produced by this strain was purified by using thin-layer chromatography. Results: The purified isolate of Aeromonas hydrophila strain produces good antimicrobial activity against Aspergillus niger, Candida albicans, Staphylococcus, Klebsiella and pseudomonas species. Conclusion: These isolates producing amylase, protease, lipase, and gelatinase enzymes, which are commercially very important and used in many industries and other biochemical sectors.

scholarly journals In vitro antimicrobial activities of several extracts endophytic Pseudomonas azotoformans UICC B-91

2021 ◽  
Vol 948 (1) ◽  
pp. 012068
Author(s):  
E Oktarina ◽  
R H Pratiwi ◽  
W Mangunwardoyo ◽  
I Hidayat ◽  
E Saepudin
Keyword(s):  
Staphylococcus Aureus ◽  
Crude Extract ◽  
Pathogenic Bacteria ◽  
Bioactive Compound ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Minimum Concentration ◽  
Layer Chromatography

Abstract Pseudomonas azotoformans known for the benefit on agriculture area as soil fertility enhancer. Pseudomonas azotoformans UICC B-91 was successfully isolated from tissues of N. altissima which traditionally known for treating infectious diseases. Therefore, endophytic P. azotoformans might contain antimicrobial compound. This research aims on exploring the antimicrobial activities of several extracts P. azotoformans UICC B-91 against pathogenic microbes. Isolates were extracted with dichloromethane, chloroform, and ethyl acetate. Each crude extract was observed for its potential antimicrobial activity with disc diffusion method against several pathogen microbes. Bioactive compound was assayed by thin layer chromatography (TLC) and screened by bioautography. Results indicated that all crude extract have inhibitory effect on Escherichia coli ATCC 8739, Bacillus cereus ATCC 10876, Staphylococcus aureus ATCC 6583, Staphylococcus aureus ATCC 25923, Salmonella typhimurium ATCC 25241, Pseudomonas aeruginosa ATCC 15442, Bacillus subtilis ATCC 19659 and Candida albicans ATCC 10231. Highest clear zone was on dichloromethane extract. TLC fraction of dichloromethane extract was able to inhibit growth on pathogenic bacteria (except for B. subtilis ATCC 19659 and S. aureus ATCC 6583) and fungi at minimum concentration in the range 125-1,000 μg/mL and 125 μg/mL, respectively. Bioactive compound from endophytic P. azotoformans extracted with dichloromethane have antibacterial and anticandidal effect.

Characterization of Antibacterial-Producing Endophytic Fungi of Syzygiumpolyanthum Leaves

2020 ◽  
Vol 20 (4) ◽  
pp. 448-454
Author(s):  
Rahmita Burhamzah ◽  
Gemini Alam ◽  
Herlina Rante
Keyword(s):  
Molecular Characterization ◽  
Endophytic Fungi ◽  
Morphological Characterization ◽  
Test Method ◽  
Analysis Method ◽  
Nitrogen Base ◽  
Rdna Genes ◽  
Antibacterial Screening ◽  
Planting Method

Background: Endophytic fungi live in plants’ tissue and can produce the same bioactive compounds as its host plant produces. Syzygiumpolyanthum leaves have known to be one of the antibacterial compound producers. Aim and Objective: This study aimed to characterize morphologically, microscopically, and molecularly the antibacterial-producing endophytic fungi of Syzygiumpolyanthum leaves. Methods: The isolation of endophytic fungi was done by fragment planting method on PDA medium. The antibacterial screening was performed using the antagonistic test as the first screening followed by the disc diffusion test method. The morphological characterization was based on isolate’s mycelia color, growth pattern, margin, and surface texture of the colony, while the microscopic characterization was based on its hyphae characteristics. The molecular characterization of the isolate was done by nitrogen base sequence analysis method on nucleotide constituent of ITS rDNA genes of the isolate. Results: The results found that isolate DF1 has antibacterial activity against E.coli, S.aureus, P.acne, and P.aeruginosa, with the greatest inhibition at 10% concentration of broth fermentation extract on S.aureus with a diameter of inhibition of 13.77 mm. Conclusion: Based on macroscopic, microscopic, and molecular characterization, DF1 isolate is similar to Ceriporialacerate.

Preparation and Characterization of Polyaniline and Ag/ Polyaniline Composite Nanoporous Particles and Their Antimicrobial Activities

2017 ◽  
Vol 26 (2) ◽  
pp. 434-442 ◽  
Author(s):  
Mohamed Shaban ◽  
Mohamed Rabia ◽  
Walid Fathallah ◽  
Neama Abd El-Mawgoud ◽  
Asmaa Mahmoud ◽  
...  
Keyword(s):  
Antimicrobial Activities ◽  
Nanoporous Particles

scholarly journals Gene Cloning and Molecular Characterization of a Two-Enzyme System Catalyzing the Oxidative Detoxification of β-Endosulfan

2002 ◽  
Vol 68 (12) ◽  
pp. 6237-6245 ◽  
Author(s):  
Tara D. Sutherland ◽  
Irene Horne ◽  
Robyn J. Russell ◽  
John G. Oakeshott
Keyword(s):  
Shuttle Vector ◽  
Open Reading Frame ◽  
Cosmid Library ◽  
Flavin Mononucleotide ◽  
Flavin Reductase ◽  
Reading Frame ◽  
Reductase Gene ◽  
Degradation Activity ◽  
Layer Chromatography

ABSTRACT The gram-positive bacterium Mycobacterium sp. strain ESD is able to use the cyclodiene insecticide endosulfan as a source of sulfur for growth. This activity is dependent on the absence of sulfite or sulfate in the growth medium. A cosmid library of strain ESD DNA was constructed in a Mycobacterium-Escherichia coli shuttle vector and screened for endosulfan-degrading activity in Mycobacterium smegmatis, a species that does not degrade endosulfan. Using this method, we identified a single cosmid that conferred sulfur-dependent endosulfan-degrading activity on the host strain. An open reading frame (esd) was identified within this cosmid that, when expressed behind a constitutive promoter in a mycobacterial expression vector, conferred sulfite- and sulfate-independent β-endosulfan degradation activity on the recombinant strain. The translation product of this gene (Esd) had up to 50% sequence identity with an unusual family of monooxygenase enzymes that use reduced flavins, provided by a separate flavin reductase enzyme, as cosubstrates. An additional partial open reading frame was located upstream of the Esd gene that had sequence homology to the same monooxygenase family. A flavin reductase gene, identified in the M. smegmatis genome, was cloned, expressed, and used to provide reduced flavin mononucleotide for Esd in enzyme assays. Thin-layer chromatography and gas chromatography analyses of the enzyme assay mixtures revealed the disappearance of β-endosulfan and the appearance of the endosulfan metabolites, endosulfan monoaldehyde and endosulfan hydroxyether. This suggests that Esd catalyzes the oxygenation of β-endosulfan to endosulfan monoaldehyde and endosulfan hydroxyether. Esd did not degrade either α-endosulfan or the metabolite of endosulfan, endosulfan sulfate.

Synthesis and chromatographic characterization of [Tc-99m]technetium-humic acid species

2000 ◽  
Vol 88 (2) ◽  
Author(s):  
D. Rößler ◽  
K. Franke ◽  
R. Süß ◽  
E. Becker ◽  
H. Kupsch
Keyword(s):  
Humic Acid ◽  
Stannous Chloride ◽  
Gel Permeation Chromatography ◽  
Paper Electrophoresis ◽  
Gel Permeation ◽  
Soil Humic Acid ◽  
Chromatographic Characterization ◽  
Ph Dependent ◽  
Layer Chromatography

A natural moor soil humic acid (HA) was labeled with Tc-99m via reduction of pertechnetate with stannous chloride. The humic acid species obtained were characterized by thin layer chromatography (TLC), gel permeation chromatography (GPC), sequential chromatographic analysis (SCA), paper electrophoresis and micropore filtration. Labeling was found to take place in all ranges of molecular weight. Due to the complex humic acid composition and the formation of hydroxo species the labeling yields strongly depend on the separation conditions, ranging from 42% to 80%. The pH-dependent distribution of mobile and immobile species was determined by SCA for HTcO

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