Iron-Sulfur-Nitrosyl Complex Increases the Resistance of Pea Seedling to Water Deficiency

Background: The binding of free iron ions in the composition of nitrosyl complexes is probably one of the mechanisms of the antioxidant action of nitric oxide. The study of the protective properties of nitric oxide is often carried out using exogenous NO donors. In our work, we used nitric oxide donor - sodium μ2-dithiosulfate-tetranitosyldiferrate tetrahydrate (TNIC-thio). Objective: The aim of our work was to investigate the possibility of using TNIC-thio to increase the resistance of plants to stress factors. Since the implementation of anti-stress programs requires a lot of energy expenditure, we studied the functional state of the mitochondria of pea seedlings under conditions of water deficiency and treatment with TNIC-thio. Materials and Methods: The functional state of the mitochondria was studied as per the level of lipid peroxidation by the spectrofluorimetry, by a fatty acid composition of mitochondrial membranes with the chromatography technique and by the morphology of mitochondria with the atomic force microscopy. Results: Water deficiency has caused changes in the Fatty Acid (FA) composition, which manifested themselves in increasing the content of saturated FAs and decreasing the content of unsaturated FAs containing 18 and 20 carbon atoms. Treatment of pea seeds with 10–8 M TNIC-thio under these conditions led to the prevention of LPO, prevention of changes in the FA composition of mitochondrial membranes and reduction in the number of swollen organelles. Conclusion: It can be assumed that the protective effect of TNIC-thio is due to the preservation of the functional state of the mitochondria.

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The Influence of Ultra-Low Concentrations of Potassium Anphen on the Bioenergetic Characteristics of Mitochondria

Current Bioactive Compounds ◽

10.2174/1573407214666181116093909 ◽

2020 ◽

Vol 16 (4) ◽

pp. 537-542

Author(s):

Zhigacheva Irina ◽

Volodkin Aleksandr ◽

Rasulov Maksud

Keyword(s):

Functional State ◽

Membrane Lipids ◽

Biological Effects ◽

Dose Dependence ◽

Stress Factors ◽

Mitochondrial Membranes ◽

Oxidation Rates ◽

Pea Seedlings ◽

Low Concentrations ◽

Wide Range

Background: One of the main sources of ROS in stress conditions is the mitochondria. Excessive generation of ROS leads to oxidation of thiol groups of proteins, peroxidation of membrane lipids and swelling of the mitochondria. In this regard, there is a need to search for preparationsadaptogens that increase the body's resistance to stress factors. Perhaps, antioxidants can serve as such adaptogens. This work aims at studying the effect of antioxidant; the potassium anphen in a wide range of concentrations on the functional state of 6 day etiolated pea seedlings mitochondria (Pisum sativum L). Methods: The functional state of mitochondria was studied per rates of mitochondria respiration, by the level of lipid peroxidation and study of fatty acid composition of mitochondrial membranes by chromatography technique. Results: Potassium anphen in concentrations of 10-5 - 10-8 M and 10-13-10-16 prevented the activation of LPO in the mitochondrial membranes of pea seedlings, increased the oxidation rates of NAD-dependent substrates and succinate in the respiratory chain of mitochondria that probably pointed to the anti-stress properties of the drug. Indeed, the treatment of pea seeds with the preparation in concentrations of 10-13 M prevented the inhibition of growth of seedlings in conditions of water deficiency. Conclusion: It is assumed that the dose dependence of the biological effects of potassium anphen and the manifestation of these effects in ultra-low concentrations are due to its ability in water solutions to form a hydrate containing molecular ensembles (structures).

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