Investigating the Antitumor Activities of Curcumae Rhizoma by Building HepG2 Tumor-bearing Nude Mice Models and Exploring its Anticancer Mechanism by Observing Glycoprotein Expression of Tumor Tissues Using Lectin Microarray Technology

2020 ◽  
Vol 01 ◽  
Author(s):  
Wei Gu ◽  
Fanwang Meng ◽  
Huangjin Tong ◽  
De Ji ◽  
Lin Li ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Nude Mice ◽  
Blood Stasis ◽  
Biological Significance ◽  
T Antigen ◽  
Protein Glycosylation ◽  
Anticancer Activities ◽  
Tumor Bearing ◽  
Tumor Tissues ◽  
Anticancer Mechanism

Background: Curcumae Rhizoma (CR) comes from Curcuma genus, functional breaking blood stasis, detumescence and acesodyne for treatment of Zhengjia accumulation, amenorrhea, traumatic injury and bruising pain. Modern pharmacological studies have shown that the main monomer compositions of Curcumae Rhizoma, such as curcumol, β-elemene, curcumin, have good anti-tumor effects. However, the mechanisms are not clear yet. Previous studies have revealed that the associated glycoprotein showed significant differences with normal people after the development of hepatocellular carcinoma (HCC). Methods: In this study, the anticancer activities of CR extract were investigated by constructing HepG2 tumor bearing nude mice models. Furthermore, glycan profiles of tumor tissues were thoroughly characterized by lectin microarrays, a high-throughput technique, in an effort to explore the anticancer mechanism of Curcumae Rhizoma. Results: It is indicated that CR extract might inhibit the tumor proliferation in tumor-bearing model and the potential mechanisms might be CR treatment altered protein glycosylation of tumor cell, which plays an important role in the pathogenesis and progression of HCC. In detail, fucosylation (identified by PSA and UEA-I), bisecting GlcNAc or multianternnary (identified by PHA-E+L) and terminal GalNAc (identified by BPL) decreased, while sialylation (identified by WGA and SNA), high-mannose (identified by ConA) and T-antigen/TN-antigen/sialyl-T antigen (identified by ACA) increased in CR treatment group compared to model group. Similar phenomenon also occurred in two positive groups, western medicine cyclophosphamide (CTX) and Chinese medicine monomer β-elemene administration groups, especially in β-elemene administration one. The liver contains various receptors on sinusoidal and hepatocyte surfaces, and many proteins that bind to these receptors reply on carbohydrate moieties during the development of HCC. Conclusion: In this point of view, a search for the biological significance of glycosylation expression and its function after Chinese Medicine administration in HCC may open a new direction in glycobiology.

scholarly journals miR-142-3p Suppresses Cell Growth by Targeting CDK4 in Colorectal Cancer

2018 ◽  
Vol 51 (4) ◽  
pp. 1969-1981 ◽  
Author(s):  
Xiangyu Zhu ◽  
Si-ping Ma ◽  
Dongxiang Yang ◽  
Yanlong Liu ◽  
Yong-peng Wang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Growth ◽  
Tumor Cell ◽  
Nude Mice ◽  
Colony Formation ◽  
Tumor Cell Growth ◽  
Rt Pcr ◽  
Tumor Tissues

Background/Aims: Deregulation of microRNAs (miRNAs) has been associated with a variety of cancers, including colorectal cancer (CRC). Here, we investigated anomalous miR-142-3p expression and its possible functional consequences in primary CRC samples. Methods: The expression of miR-142-3p was measured by quantitative RT-PCR in 116 primary CRC tissues and adjacent non-tumor tissues. The effect of miR-142-3p up- or down-regulation in CRC-derived cells was evaluated in vitro by cell viability and colony formation assays and in vivo by growth assays in xenografted nude mice. Results: Using quantitative RT-PCR, we found that miR-142-3p was down-regulated in 78.4 % (91/116) of the primary CRC tissues tested when compared to the adjacent non-tumor tissues. We also found that the miR-142-3p mimic reduced in vitro cell viability and colony formation by inducing cell cycle arrest in CRC-derived cells, and inhibited in vivo tumor cell growth in xenografted nude mice. Inversely, we found that the miR-142-3p inhibitor increased the viability and colony forming capacity of CRC-derived cells and tumor cell growth in xenografted nude mice. In addition, we identified CDK4 as a potential target of miR-142-3p by predictions and dual-luciferase reporter assays. Concordantly, we found that miR-142-3p mimics and inhibitors could decrease and increase CDK4 protein levels in CRC-derived cells, respectively. Conclusion: From our results we conclude that miR-142-3p may act as a tumor suppressor in CRC and may serve as a tool for miRNA-based CRC therapy.

scholarly journals An Empirical Case of Chinese Medicine in the Treatment of Idiopathic Edema

2021 ◽  
Vol 5 (6) ◽  
pp. 44-46
Author(s):  
Xiaojuan Wang ◽  
Xiaohui Li
Keyword(s):  
Chinese Medicine ◽  
Clinical Experience ◽  
Clinical Condition ◽  
Blood Stasis ◽  
Idiopathic Edema

Idiopathic edema is a common, challenging, and complicated clinical condition with no recognized cause that is easy to prolong and repeat. Professor Li has a lot of expertise treating this condition. This condition is associated with a yang shortage in the spleen and kidneys, as well as a mutual accumulation of water and blood stasis. Zhenwu Decoction and Guizhi Fuling tablet are added to the prescription. Ms. Li’s clinical experience in the treatment of idiopathic edema is being shared with the fellow.

scholarly journals TRPM2 promotes pancreatic cancer by PKC/MAPK pathway

2020 ◽  
Author(s):  
Rui Lin ◽  
Xunxia Bao ◽  
Hui Wang ◽  
Sibo Zhu ◽  
Zhongyan Liu ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Nude Mice ◽  
Transcriptome Analysis ◽  
Cell Model ◽  
Mapk Pathway ◽  
Tissue Sample ◽  
Pancreatic Cancer Cell Line ◽  
Cancer Cell Line ◽  
Migration And Invasion ◽  
Tumor Bearing

AbstractBackgroundThe mechanism of pancreatic cancer(PA) is not fully understanded. In our last report, TRPM2 plays a promising role in pancreatic cancer. However, the mechanism of TRPM2 is still unknown in this dismal disease. This study was designed to investigate the role and mechanism of TRPM2 in pancreatic cancer.MethodsTRPM2 overexpressed and siRNA plasmid were created and transfected with pancreatic cancer cell line(BxPC-3) to construct the cell model. We employed CCK-8, Transwell, scratch wound, and nude mice tumor bearing model to investigate the role of TRPM2 in pancreatic cancer. Besides, we collected the clinical data, tumor tissue sample(TT) and para-tumor sample(TP) from the pancreatic cancer patients treated in our hospital. We analyzed the mechanism of TRPM2 in pancreatic cancer by transcriptome analysis, Westernblot, and PCR.ResultsOverexpressed TRPM2 could promote pancreatic cancer in proliferation, migration, and invasion ability in no matter the cell model or nude mice tumor bearing model. TRPM2 level is highly negative correlated to the overall survival and progression-free survival time in PA patients, however, it is significantly increased in PA tissue as the tumor stage increases. The transcriptome analysis, GSEA analysis, Westernblot, and PCR results indicates TRPM2 is highly correlated with PKC/MAPK pathways.ConclusionTRPM2 could directly activate PKCα by calcium or indirectly activate PKCε and PKCδ by increased DAG in PC, which promote PC by downstream MAPK/MEK pathway activation.

scholarly journals On the Treatment of Kidney Fibrosis from the Theory of Internal Deficiency of Qi and the Coexistence of Phlegm and Blood Stasis

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Liu Min ◽  
Leng Wei
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Theoretical Basis ◽  
Renal Fibrosis ◽  
Blood Stasis ◽  
Point Of View ◽  
Kidney Fibrosis ◽  
Cause And Effect ◽  
Pathological Characteristics ◽  
Chinese Medicine Treatment

Traditional Chinese medicine believes that the etiology and pathogenesis of renal fibrosis are characterized by deficiency of the lung, spleen and kidney, and phlegm, blood stasis, dampness and poison. The positive and the evil can influence each other and cause and effect each other, forming the pathological characteristics of the deficiency, the deficiency, the deficiency and the reality. Chinese medicine treatment of the disease has its unique advantages, external and internal injury equal emphasis, correction and dispelling evil and regulation. From the point of view of "deficiency of qi and coexistence of phlegm and blood stasis", the treatment of renal fibrosis can provide theoretical basis for the treatment of the disease.

LINC01133 hampers the development of gastric cancer through increasing SST via binding to microRNA-576-5p

Epigenomics ◽  
2021 ◽  
Author(s):  
Leiyi Zhang ◽  
Ke Pan ◽  
Zhongkun Zuo ◽  
Fei Ye ◽  
Ding Cao ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Tumor Growth ◽  
Nude Mice ◽  
Therapeutic Targets ◽  
Loss Of Function ◽  
Gain Of Function ◽  
Tumor Bearing ◽  
New Therapeutic Targets ◽  
Low Expression ◽  
Development Of Gastric Cancer

Aim: Our study aimed at investigating how LINC01133 functions in gastric cancer (GC) progression. Materials & methods: Gain-of-function and loss-of-function approaches were applied to analyze the effects of LINC01133, microRNA-576-5p (miR-576-5p) and somatostatin (SST) on the biological behaviors of GC cells and in tumor-bearing nude mice. Results: GC tissues and cells showed low expression of LINC01133, and LINC01133 overexpression decreased malignant phenotypes of GC cells. Moreover, LINC01133 upregulated SST through binding to miR-576-5p. Overexpressing miR-576-5p or suppressing SST reversed the functions of LINC01133 in biological potentials of GC cells and tumor growth. Conclusion: LINC01133 overexpression may inhibit GC development by downregulation of miR-576-5p and upregulation of SST, which suggests new therapeutic targets for GC.

Large-scale production of polyoma middle T antigen by using genetically engineered tumors

1985 ◽  
Vol 5 (7) ◽  
pp. 1795-1799
Author(s):  
D R Kaplan ◽  
B Bockus ◽  
T M Roberts ◽  
J Bolen ◽  
M Israel ◽  
...  
Keyword(s):  
Nude Mice ◽  
Large Scale ◽  
T Antigen ◽  
Genetically Engineered ◽  
Scale Production ◽  
Large Scale Production ◽  
Metallothionein Promoter ◽  
Polyoma Middle T Antigen ◽  
Nih 3T3

A recombinant plasmid containing a metallothionein promoter-polyoma middle T cDNA fusion was constructed and used to transfect NIH 3T3 cells. Transformed cells expressing middle T were injected into nude mice. Within 3 weeks, each mouse produced tumors containing middle T equivalent to that in 250 to 1,000 100-mm dishes of polyomavirus-infected cells. This middle T, partially purified by immunoaffinity chromatography, retained activity as measured by its ability to be phosphorylated in vitro. The combined approach of fusing strong promoters to genes of interest and utilizing nude mice to grow large quantities of cells expressing the gene provides a quick, inexpensive alternative to other expression systems.

Sign in / Sign up

Export Citation Format

Share Document