Determination of iron content in selected indigenous green leafy vegetables in Baraton, Kenya.

2016 ◽  
Vol 5 (04) ◽  
pp. 4500 ◽  
Author(s):  
Anthoney Swamy T* ◽  
Nyabwari Loice Kerubo ◽  
Jackie K. Obey ◽  
Magut Hillary
Keyword(s):  
Spectrophotometric Method ◽  
Iron Concentration ◽  
Leafy Vegetables ◽  
Test Analysis ◽  
Absorbance Measurement ◽  
Green Leafy Vegetables ◽  
Significant Difference ◽  
Indigenous Vegetables ◽  
Spider Plant

A study was done to determine the level of iron in selected indigenous vegetables using UV-vis spectrophotometric method. The four indigenous vegetables that was frequently consumed by Baraton families were; amaranths, pumpkin leaves, spider plant, and bacella alba, were selected for the study. Absorbance measurement was done on the ash samples of cooked and uncooked vegetables using UV-Vis spectrophotometer at 458 nm.  The results showed among the uncooked vegetables, the iron concentration ranged from 0.081 to 0.23 ppm with pumpkin leaves and baccela alba recording the lowest and highest level respectively. The cooked vegetables had Fe levels ranging for 0.041 ppm to 0.43 ppm with pumpkin leaves and amaranths having lowest and highest Fe levels respectively.  One sample t-test analysis showed that there was no significant difference between the Fe levels in the cooked and uncooked vegetables P>0.05. The findings showed that the sampled vegetables are a source of iron in diet.

scholarly journals New Kinetic Spectrophotometric Method for Determination of Folic Acid in Pharmaceutical Formulations

2015 ◽  
Vol 50 ◽  
pp. 169-178
Author(s):  
Mouhammed Khateeb ◽  
Basheer Elias ◽  
Fatema Al Rahal
Keyword(s):  
Folic Acid ◽  
Spectrophotometric Method ◽  
Pharmaceutical Formulations ◽  
Fixed Time ◽  
Rate Data ◽  
Sulfuric Acid Medium ◽  
Significant Difference ◽  
Kinetic Spectrophotometric ◽  
Comparison Of The Results

A simple and sensitive kinetic spectrophotometric method has been developed for the determination of folic acid (FA) in bulk and pharmaceutical Formulations. The method is based on the oxidation of FA by Fe (III) in sulfuric acid medium. Fe (III) subsequently reduces to Fe (II) which is coupled with potassium ferricyanide to form Prussian blue. The reaction is followed spectrophotometrically by measuring the increase in absorbance at λmax 725 nm. The rate data and fixed time methods were adopted for constructing the calibration curves. The linearity range was found to be 1–20 μg mL-1 for each method. The correlation coefficient was 0.9978 and 0.9993, and LOD was found to be 0.91 and 0.09 μg mL-1 for rate data and fixed time methods, respectively. The proposed method has been successfully applied to the determination of FA in formulations with no interference from the excipients. Statical comparison of the results shows that there is no significant difference between the proposed and pharmacopoeial methods

Development and Validation of an HPLC Method for the Determination of Thiamine and Riboflavin in Green Leafy Vegetables Using Clara-Diastase

2011 ◽  
Vol 76 (4) ◽  
pp. C639-C642 ◽  
Author(s):  
Alicia del Carmen Mondragón-Portocarrero ◽  
Lourdes Vázquez-Odériz ◽  
MaÁngeles Romero-Rodríguez
Keyword(s):  
Hplc Method ◽  
Leafy Vegetables ◽  
Green Leafy Vegetables ◽  
Development And Validation

scholarly journals Determination of vitamin A content from selected Nigerian fruits using spectrophotometric method

2017 ◽  
Vol 52 (2) ◽  
pp. 153-158 ◽  
Author(s):  
SO Aremu ◽  
CC Nweze
Keyword(s):  
Vitamin A ◽  
Quantitative Determination ◽  
Spectrophotometric Method ◽  
Fruits And Vegetables ◽  
Significant Difference ◽  
Conversion Formula ◽  
Major Nutrients

The vitamin A content of the fresh fruits was determined by the extraction and quantitative determination of the pro-vitamin A carotenoid-?-carotene. The Retinol Equivalent (RE) was then obtained using standard conversion formula. The result indicated that the fruit with the minimum vitamin A content and percentage among the six samples was found in mango 301.61 ± 1.03 (7%), which was followed closely by watermelon 350.12 ± 19.01 (8%), guava 504.1 ± 0.75 (11%), tomato 542.86 ± 20.20 (12%), pawpaw 683.93 ± 5.15 (16%) and the highest content was found in carrot 2054.10 ± 1.28 (46%) in (RE/100 g). Statistical calculations and analysis showed that there was significant difference in vitamin A content among the fruits (p<0.05). Thus, besides major nutrients fruits and vegetables contribute very good amount of micro nutrient-vitamin A from ?-carotene.Bangladesh J. Sci. Ind. Res. 52(2), 153-158, 2017

scholarly journals Development of an Ultraviolet Spectrophotometric Method for the Determination of Ceftiofur Sodium Powder

2009 ◽  
Vol 92 (6) ◽  
pp. 1673-1680 ◽  
Author(s):  
marinês J E Souza ◽  
Natália Canedo ◽  
Paulo S Souza Filho ◽  
Ana M Bergold
Keyword(s):  
Spectrophotometric Method ◽  
Spectrophotometric Analysis ◽  
Uv Spectrophotometry ◽  
Accuracy And Precision ◽  
Significant Difference ◽  
Student’S T ◽  
Ceftiofur Sodium ◽  
Interday Precision ◽  
Student’S T Test

Abstract A UV spectrophotometric method was developed for determination of ceftiofur sodium in the drug substance and sterile powder for injection. The method validation, which yielded good results, included evaluation of the range, linearity, intraand interday precision, accuracy, recovery, specificity, robustness, LOQ, and LOD. The UV spectrophotometric determinations were performed at 292 nm. Good linearity was obtained between 2.5 and 20.0 g/mL. A prospective validation showed that the method is linear (r = 0.9999) and precise, with RSD values of 0.3 for product A and 0.4 for product B. The intraand interday precision values were &lt;2 for all samples analyzed. Comparison of UV spectrophotometry and LC by analysis of variance and Student's t-test showed no significant difference between methodologies. Moreover, the accuracy and precision obtained with the UV method correlated well with the values obtained with the LC method, and this correlation suggests that UV spectrophotometric analysis can be an inexpensive, reliable, and less time-consuming alternative to chromatographic analysis. The results demonstrated the validity of the proposed method as a simple and useful alternative for the determination of ceftiofur in routine QC analyses.

The recalcitrance of oxalate, nitrate and nitrites during the controlled lactic fermentation of commonly consumed green leafy vegetables

2015 ◽  
Vol 45 (2) ◽  
pp. 336-346 ◽  
Author(s):  
A. Jagannath ◽  
Manoranjan Kumar ◽  
P S Raju
Keyword(s):  
High Performance ◽  
Leafy Vegetables ◽  
Spontaneous Fermentation ◽  
Content Type ◽  
Lactic Fermentation ◽  
Green Leafy Vegetables ◽  
Beneficial Effects ◽  
Balanced Diet ◽  
Significant Difference ◽  
Nitrate And Nitrite

Purpose – Green leafy vegetables (GLVs) are important components of a balanced diet especially in developing countries where the major requirements of micronutrients are met. However, GLVs also contain significant amounts of oxalate, nitrate and nitrites, whose role in the human diet is constantly changing. The current study explored the behavior of nitrate, nitrites and oxalate in lactic-fermented GLVs with an intention to develop functional foods based on them. Design/methodology/approach – Selected strains of beneficial lactic acid bacteria were used for the controlled fermentation of GLV, while an identical portion was subjected to spontaneous fermentation. The nitrate and nitrites were monitored spectrophotometrically, while oxalate contents were quantified by both titrimetric and by high-performance liquid chromatography throughout the duration of fermentation. Findings – More than 90 per cent of individual constituents studied remained intact in the GLVs paste after the six-day controlled fermentation period. However, there was significant difference between the controlled and spontaneously fermented samples in terms of oxalate, nitrate and nitrite contents. Originality/value – Controlled lactic fermentation although superior in all other aspects may not be able to lower the anti-nutrients present. The advantages of spontaneous fermentation vis-à-vis controlled fermentation are discussed. The work will bring out the importance of the beneficial effects of GLVs and the effect of lactic fermentation.

scholarly journals Sequential determination of fat- and water-soluble vitamins in green leafy vegetables during storage

2012 ◽  
Vol 1261 ◽  
pp. 179-188 ◽  
Author(s):  
J. Santos ◽  
J.A. Mendiola ◽  
M.B.P.P. Oliveira ◽  
E. Ibáñez ◽  
M. Herrero
Keyword(s):  
Water Soluble ◽  
Leafy Vegetables ◽  
Sequential Determination ◽  
Green Leafy Vegetables

scholarly journals A Sensitive Spectrophotometric Method for the Determination of Desloratadine in Tablets

2007 ◽  
Vol 90 (2) ◽  
pp. 372-375 ◽  
Author(s):  
Sena Çğalar ◽  
Aysel Öztun
Keyword(s):  
Spectrophotometric Method ◽  
Control Analysis ◽  
Optimum Conditions ◽  
Chromatography Method ◽  
Deep Blue ◽  
Quality Control Analysis ◽  
Significant Difference ◽  
Liquid Chromatography Method ◽  
First Time

Abstract A simple, rapid, and sensitive visible spectrophotometric method was developed, for the first time, for analysis of desloratadine (DE) in tablets. The method is based on the deep-blue colored TCNQ- radical anion formed by interaction of the drug (n-donor) with 7,7,8,8-tetracyanoquinodimethane (TCNQ, π-acceptor) in acetonitrile at ambient temperature. Optimum conditions for the reaction were investigated, absorbances were read at 843 nm, and the linearity range for concentrations of DE was found to be 1.5-13 μg/mL. The reaction product remains stable up to 8 h when kept at room temperature in the dark. The developed method was validated and successfully applied to the determination of DE in tablets. The tablets were also analyzed with a column liquid chromatography method reported in literature. The results from both methods were statistically compared by t- and F-tests. No significant difference was found for the means and standard deviations at 95% confidence level. Accuracy was examined through recovery studies. Being very simple and reliable, the method can be recommended for routine quality control analysis of DE in tablets.

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