Enzyme modulation studies of Fumaronitrile on fresh water fish Oreochromis mossambicus

2016 ◽  
Vol 5 (11) ◽  
pp. 5050
Author(s):  
Chinnadurai Kuppusamy ◽  
Arul Ganesh ◽  
Karthikeyeni Sambanthan ◽  
Eyini Muthukumarasamy ◽  
Balaji Paulraj*

The present study was directed to analyse the potential enzymatic variation efficiency of fumaronitrile on fresh water fish tilapia (Oreochromis mossambicus). To study the acute toxicity of xenobiotic five different concentrations (20, 40, 60, 80 and 100ppb) of fumaronitrile along with control group was maintained for over 96 hrs. At every 1 hr. fishes was monitor both control and treatment groups. The LC50 value was found to be as 60ppb. To study the chronic toxicity of xenobiotic three different concentrations (2ppb, 4ppb and 6ppb) of fumaronitrile along with control group was maintained for over 100 days. At 60, 80,100 days fishes was sacrificed both control and treatment groups and the organs such as liver were collected to analyse the changes in testicular enzyme activities and an increasing pattern for both antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD) was observed. Enzyme assay is one of the important methods to evaluate the toxicity of xenobiotic. At present, no report is available on the effect of fumaronitrile on antioxidant enzymes and testicular enzyme activities. This study focus light on the effects of fumaronitrile in antioxidant enzymes CAT, SOD and testicular enzymes ALP, ACP, LDH and SDH in Oreochromis mossambicus.

GIS Business ◽  
2019 ◽  
Vol 14 (6) ◽  
pp. 224-229
Author(s):  
A. Solaiappan ◽  
J. Prakash Sahaya Leon

The toxic effect of Monocrotophos on some biochemical parameters such as Protein and Amino acid in freshwater fish Oreochromis mossambicus were studied. The fishes exposed to 1/10th, 1/20th, and 1/30th sublethal concentration of Monocrotophos for 30 days. The tissue samples of gill, liver were analysed at the end of the 30th day exposure period. A decreased value of Protein and Amino acid were observed in 1/10th, 1/20th, and 1/30th sub lethal concentration of Monocrotophos at the exposure period of 30 days while comparing with the control group. The high variation observed in the 1/10th sublethal level of 30thday exposure. While compare with control group the effects of Monocrotophos on fish found during this experiment showed that the level of protein and amino acid level in various tissues (gill and liver) of Oreochromis mossambicus decreased.


2005 ◽  
Vol 19 (2) ◽  
pp. 351-355 ◽  
Author(s):  
V. Palanivelu ◽  
K. Vijayavel ◽  
S. Ezhilarasibalasubramanian ◽  
M.P. Balasubramanian

Author(s):  
Obaiah Jamakala

Objective: Cadmium (Cd) is one of the most hazardous heavy metals in aquatic environments and could threaten aquatic organisms including fish. The present study was carried out to know the protective effects of calcium (Ca) and selenium (Se) in reducing the Cd bioaccumulation in selected tissues of fresh water fish, Oreochromis mossambicus. Methods: The fresh water fish, Oreochromis mossambicus (Tilapia) was brought from the local ponds and were allowed for acclimatization to the laboratory conditions. After acclimatization, fish were exposed to sublethal concentration of Cd (1/10th of LC50/48h, i.e., 5 ppm) for 7, 15 and 30 d (d) period. 15d Cd-exposed fish were divided into three groups. The first group of fish were subjected to Ca (1 ppm) supplementation, second group received only Se (1 ppm) supplement and third group of fish were supplemented with the combination of both Ca and Se at the above said doses and observed for 7, 15 and 30d time periods. After specific time intervals, liver, kidney, gill and intestine tissues were isolated and used for Cd bioaccumulation studies. Results: Cd concentration levels significantly (P<0.05) increased in the test tissues with increased period of exposure. Maximum Cd accumulation was found in 30d Cd-exposed fish kidney tissue (22.611±0.676 μg/gm wet wt. of the tissue). However, after supplementing with Ca and/or Se, there was a significant reversal in the levels of Cd concentration in all the test tissues. Maximum reduction was observed under Ca alone supplementation. Conclusion: The present study clearly reveals that individual supplementation of Ca tends to detoxify the Cd body burden in the test tissues than the other modes of supplementation.


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