Understanding Self-Association and Tubule Formation in Phytosterol-Based Oleogels

2020 ◽  
Author(s):  
Stephen Euston ◽  
Paul Clegg ◽  
George Dalkas ◽  
Andrew Matheson
Keyword(s):  
Tubule Formation ◽  
Self Association

Aggregated, Conformationally Changed Fibrinogen Exposes the Stimulating Sites for t-PA-Catalysed Plasminogen Activation

1996 ◽  
Vol 75 (02) ◽  
pp. 326-331 ◽  
Author(s):  
Unni Haddeland ◽  
Knut Sletten ◽  
Anne Bennick ◽  
Willem Nieuwenhuizen ◽  
Frank Brosstad
Keyword(s):  
Conformational Changes ◽  
Gel Permeation Chromatography ◽  
Tissue Type ◽  
Plasminogen Activation ◽  
Chromogenic Substrate ◽  
Type Plasminogen Activator ◽  
Gel Permeation ◽  
Self Association ◽  
Fibrin Monomers ◽  
Stimulation Of

SummaryThe present paper shows that conformationally changed fibrinogen can expose the sites Aα-(148-160) and γ-(312-324) involved in stimulation of the tissue-type plasminogen activator (t-PA)-catalysed plasminogen activation. The exposure of the stimulating sites was determined by ELISA using mABs directed to these sites, and was shown to coincide with stimulation of t-PA-catalysed plasminogen activation as assessed in an assay using a chromogenic substrate for plasmin. Gel permeation chromatography of fibrinogen conformationally changed by heat (46.5° C for 25 min) demonstrated the presence of both aggregated and monomeric fibrinogen. The aggregated fibrinogen, but not the monomeric fibrinogen, had exposed the epitopes Aα-(148-160) and γ-(312-324) involved in t-PA-stimulation. Fibrinogen subjected to heat in the presence of 3 mM of the tetrapeptide GPRP neither aggregates nor exposes the rate-enhancing sites. Thus, aggregation and exposure of t-PA-stimulating sites in fibrinogen seem to be related phenomena, and it is tempting to believe that the exposure of stimulating sites is a consequence of the conformational changes that occur during aggregation, or self-association. Fibrin monomers kept in a monomeric state by a final GPRP concentration of 3 mM do not expose the epitopes Aα-(148-160) and γ-(312-324) involved in t-PA-stimulation, whereas dilution of GPRP to a concentration that is no longer anti-polymerizing, results in exposure of these sites. Consequently, the exposure of t-PA-stimulating sites in fibrin as well is due to the conformational changes that occur during selfassociation.

Self-association of insulin. Its pH dependence and effect of plasma

Diabetes ◽  
1987 ◽  
Vol 36 (3) ◽  
pp. 261-264 ◽  
Author(s):  
E. Helmerhorst ◽  
G. B. Stokes
Keyword(s):  
Ph Dependence ◽  
Self Association

RPS24c Isoform Facilitates Tumor Angiogenesis Via Promoting the Stability of MVIH in Colorectal Cancer

2020 ◽  
Vol 20 (5) ◽  
pp. 388-395 ◽  
Author(s):  
Yue Wang ◽  
Youjun Wu ◽  
Kun Xiao ◽  
Yingjie Zhao ◽  
Gang Lv ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Ribosomal Protein ◽  
Real Time ◽  
Tumor Angiogenesis ◽  
Real Time Pcr ◽  
Molecular Mechanisms ◽  
Migration And Invasion ◽  
Binding Interaction ◽  
Tubule Formation ◽  
The Stability

Background: Colorectal cancer (CRC) is the second leading cause of death worldwide, and distant metastasis is responsible for the poor prognosis in patients with advanced-stage CRC. RPS24 (ribosomal protein S24) as a ribosomal protein, multiple transcript variant encoding different isoforms have been found for this gene. Our previous studies have demonstrated that RPS24 is overexpressed in CRC. However, the mechanisms underlying the role of RPS24 in tumor development have not been fully defined. Methods: Expression of RPS24 isoforms and lncRNA MVIH in CRC tissues and cell lines were quantified by real-time PCR or western blotting assay. Endothelial tube formation assay was performed to determine the effect of RPS24 on tumor angiogenesis. The cell viability of HUVEC was determined by MTT assay, and the migration and invasion ability of HUVEC were detected by transwell assay. PGK1 secretion was tested with a specific ELISA kit. Results: Here, we found that RPS24c isoform was a major contributor to tumor angiogenesis, a vital process in tumor growth and metastasis. Real-time PCR revealed that RPS24c isoform was highly expressed in CRC tissues, while other isoforms are present in both normal and CRC tissues with no statistical difference. Moreover the change of RPS24 protein level is mainly due to the fluctuation of RPS24c. Furthermore, we observed that silencing RPS24c could decrease angiogenesis by inhibiting tubule formation, HUVEC cell proliferation and migration. Additionally, we investigated the molecular mechanisms and demonstrated that RPS24c mRNA interacted with lncRNA MVIH, the binding-interaction enhanced the stability of each other, thereby activated angiogenesis by inhibiting the secretion of PGK1. Conclusion: RPS24c facilitates tumor angiogenesis via the RPS24c/MVIH/PGK1 pathway in CRC. RPS24c inhibition may be a novel option for anti-vascular treatment in CRC.

Self-association of antipyrine and its derivatives in aqueous solutions at 40 °C via osmometry

1976 ◽  
Vol 41 (8) ◽  
pp. 2353-2364 ◽  
Author(s):  
F. Kopecký ◽  
M. Vojteková ◽  
A. Dymeš
Keyword(s):  
Aqueous Solutions ◽  
Self Association

Coadsorption of bulky ions by surfactants. Monomolecular layers of tris(2,2'-bipyridyl)ruthenium(II) complex with sodium lauryl sulphate on glassy carbon, platinium, n-SnO2, and n-Si electrodes

1984 ◽  
Vol 49 (10) ◽  
pp. 2187-2196 ◽  
Author(s):  
Jan Lasovský ◽  
František Grambal ◽  
Miroslav Rypka
Keyword(s):  
Glassy Carbon ◽  
Complex Cation ◽  
Radiant Energy ◽  
Electric Energy ◽  
Sodium Lauryl Sulphate ◽  
Surface Films ◽  
Order Of Magnitude ◽  
Monomolecular Layers ◽  
Self Association ◽  
Semiconductor Electrodes

The electrochemical and photochemical behaviour of tris(2,2'-bipyridyl)ruthenium(II) complex (I) on glassy carbon, platinium, n-SnO2, and n-Si electrodes in the presence of sodium lauryl sulphate (II) was investigated. The surfactant in low concentrations induces self-association of the complex cation and its accumulation in the electrode-solution interface. At the optimum concentrations of sodium lauryl sulphate (cII ~0.6 mmol l-1) and of the complex (cI < 0.1 mmol l-1), monomolecular layers composed of I, II counterions are formed on the electrodes. The formation of the surface films does not depend on the kind of the electrode and improves the sensitivity of the voltammetric determination of I by as much as an order of magnitude. For the semiconductor electrodes, the surface films enhance the efficiency of conversion of radiant energy into electric energy. The effect under study may participate in the photosynthesis of green plants.

Met -/- kidneys express epithelial cells that chemotax and form tubules in response to EGF receptor ligands

1997 ◽  
Vol 272 (2) ◽  
pp. F222-F228
Author(s):  
C. Kjelsberg ◽  
H. Sakurai ◽  
K. Spokes ◽  
C. Birchmeier ◽  
I. Drummond ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epithelial Cell ◽  
Egf Receptor ◽  
Transforming Growth Factor ◽  
Growth Factor Receptor ◽  
Receptor Ligands ◽  
Tubule Formation ◽  
Human Papillomavirus 16 ◽  
Immortalized Cells

The growth factor/receptor combination of hepatocyte growth factor (HGF)/c-met has been postulated to be critical for mesenchymal-to-epithelial conversion and tubule formation in the developing kidney. We therefore isolated and immortalized cells from embryonic kidneys of met -/- transgenic mice to determine whether these cells were epithelial and able to chemotax and form tubules in vitro. The cells were immortalized with retrovirus expressing human papillomavirus 16 (HPV 16) E6/E7 genes. Two rapidly dividing clones were isolated and found to express the epithelial cell markers cytokeratin, zonula occludens-1, and E-cadherin but not to express the fibroblast marker vimentin. The met -/- cells were able to chemotax in response to epidermal growth factor and transforming growth factor-alpha (TGF-alpha) and form tubules in vitro in response to TGF-alpha but not HGF. These experiments suggest that the HGF/c-met axis is not essential for epithelial cell development in the embryonic kidney and demonstrate that other growth factors are capable of supporting early tubulogenesis.

scholarly journals Characterization of Native Reversible Self-Association of a Monoclonal Antibody Mediated by Fab-Fab Interaction

2020 ◽  
Vol 109 (1) ◽  
pp. 443-451 ◽  
Author(s):  
Lorenzo Gentiluomo ◽  
Dierk Roessner ◽  
Werner Streicher ◽  
Sujata Mahapatra ◽  
Pernille Harris ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Self Association

scholarly journals Weak self-association of cytochrome c peroxidase molecules observed by paramagnetic NMR

2016 ◽  
Vol 65 (1) ◽  
pp. 29-40 ◽  
Author(s):  
Jesika Schilder ◽  
Marcellus Ubbink
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Peroxidase ◽  
Paramagnetic Nmr ◽  
Self Association

scholarly journals The influence of bile salt structure on self-association in aqueous solutions.

1983 ◽  
Vol 258 (10) ◽  
pp. 6362-6370 ◽  
Author(s):  
A Roda ◽  
A F Hofmann ◽  
K J Mysels
Keyword(s):  
Aqueous Solutions ◽  
Bile Salt ◽  
Salt Structure ◽  
Self Association
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