Identification of Dominant Bacteria Isolated From Periodontal Abscesses

Aim: Various methods investigating the bacterial content causing periodontal abscesses have been applied in studies conducted until today. However, these studies have focused on periodontopathogens. Our study was carried out to research whether different pathogens other than the known periodontopathogens are present in periodontal abscess formation. Therefore, dominant bacterial samples obtained from the periodontal abscess content using the culture-dependent method were identified by 16S rDNA sequencing. Materials and Methods: Samples were obtained using a syringe or a periopaper from periodontal abscesses of 20 volunteers who met the research criteria. The three different bacterial colonies that were observed most intensely in each sample were selected and purified, and the isolates obtained were kept until the next characterization. Genomic DNA was isolated from each isolate; 16S rRNA genes were amplified by polymerase chain reaction and identified using DNA sequencing analyses. Results: As a result of culture-dependent methods, bacterial species belonging to Streptococcus, Staphylococcus, Neisseria, Actinomyces, Morococcus, Moraxella, and Enterococcus genera were isolated from a total of 60 bacterial isolates, three of which were the most densely growing colonies from each periodontal abscess sample. Conclusion: In our study, most of the bacterial species detected were identified for the first time in the bacterial content of periodontal abscesses. In some previously done studies, most of these bacteria species were shown to cause abscesses in different parts of the body. It was concluded that further studies are needed to determine the number and proportion of these bacteria species in total bacterial content to evaluate whether they cause periodontal abscesses.

The Relationship between Natural Radioactivity of Al-Rohban Soil in Al-Najaf Governorate and Its Microbial Content

The present study was designed to explore the relationship between radioactivity at Al-Rohban soil in Al-Najaf Governorate, located 30 km away from Najaf city center, and its microbial content. The radiological survey was conducted by γ–ray spectrometry, using purity Germanium (HPGe) detector. A selected surface soil layer (10 cm depth, 50 and 100 m expansion) was tested. The physical analyses were conducted in the Ministry of Environment, Center for Prevention of Radiation. The results showed that the estimated concentrations of Bi-214, Ra-226, Ac-228, Th-232, K-40 and Cs-137 were 47.93, 81.87, 5.03, 1.63, 126.3 and 3.5 Bq/Kg, respectively. Isotopes average concentrations were equivalent to the lowest specified International Atomic Energy standards. As related to the analysis of bacterial content in the soil sample, the total cell count (in cells per gram of soil) in the different areas studied (R1, R2, R3 and R4) had values of 70000, 200, 60000 and 300 cell/gm., respectively. The statistical analysis of these results revealed no relationship between radioactivity and microorganisms existence.

Shifts in the Oral Microbiota During a Four-Week Commercial Saturation Dive to 200 Meters

During commercial saturation diving, divers live and work under hyperbaric and hyperoxic conditions. The myriads of bacteria that live in and on the human body must adjust to the resultant hyperbaric stress. In this study, we examined the shifts in bacterial content in the oral cavity of saturation divers, using a metagenomic approach to determine the diversity in the composition of bacterial phyla and genera in saliva from 23 male divers before, during, and immediately after 4 weeks of commercial heliox saturation diving to a working depth of circa 200 m. We found that the bacterial diversity fell during saturation, and there was a change in bacterial composition; with a decrease at the phylum level of obligate anaerobe Fusobacteria, and an increase of the relative abundance of Actinobacteria and Proteobacteria. At the genus level, Fusobacterium, Leptotrichia, Oribacterium, and Veillonella decreased, whereas Neisseria and Rothia increased. However, at the end of the decompression, both the diversity and composition of the microbiota returned to pre-dive values. The results indicate that the hyperoxic conditions during saturation may suppress the activity of anaerobes, leaving a niche for other bacteria to fill. The transient nature of the change could imply that hyperbaric heliox saturation has no lasting effect on the oral microbiota, but it is unknown whether or how a shift in oral bacterial diversity and abundance during saturation might impact the divers’ health or well-being.

Circulating microbial content in myeloid malignancy patients carries diagnostic and prognostic potential

Although recent work has characterized the microbiome in solid tumors, microbial content in hematological malignancies is not well-characterized. Here we analyzed existing deep DNA sequence data from the blood and bone marrow of 1,870 patients with myeloid malignancies, along with healthy controls, for bacterial, fungal, and viral content. After strict quality filtering, we find evidence for dysbiosis in disease cases, and distinct microbial signatures among diagnoses. In patients with low-risk myelodysplastic syndrome, we provide evidence that Epstein-Barr infection status refines risk stratification into more precise categories than the current standard. Motivated by these observations, we construct machine-learning classifiers that can discriminate among disease subtypes based solely on bacterial content. Our study highlights the potential of the circulating microbiome as a diagnostic and prognostic tool.

Bioremediation of Clay With High Oil Content and Biological Response After Restoration

The clay with high oil content form soil lumps, which is hard for microbes to repair. In this paper, the bioremediation + biostimulation was applied to improve the bioremediation effect of the soil with high oil content, that modified by local cow dung and sandy soil, the ecological toxicity of the soil after restoration was further analyzed. After 53 days of bioremediation, the degradation efficiency with respect to the total petroleum hydrocarbon (TPH) content reached 76.9% ± 2.2%; the soil bacterial content reached 4.9 × 107 CFU/g soil and the results were better than those in the natural attenuation M1group of experimental soils. The relative abundances of petroleum-degrading bacteria added to M5 remained high (Achromobacter 9.44%, Pseudomonas 31.06%, and Acinetobacter 14.11%), and the proportions of some other indigenous bacteria (Alcanivorax and Paenibacillus) also increased. The toxicity of the bioremediated soil was reduced by seed germination and earthworm survival experiments.

Study on the Dose-effect Relationship of Moxa Burning Fumigation in Air Disinfection in Traditional Chinese Medicine Clinics

Objective — It’s to study the dose-effect relationship of moxa produced by Gansu Baicao Group in the air disinfection in traditional Chinese medicine clinics. Method — Use moxa sticks burning fumigation to disinfect indoor air in the clinics of traditional Chinese medicine experts; use the natural sedimentation method to detect the bacterial content in the air; compare the sterilization effects of 0.5, 1 and 1.5 moxa sticks 0 h, 1 h and 2 h after air disinfection. Results — In the 54m3 TCM clinic, there was no significant difference in the sterilization rate of air bacteria 0h, 1h, and 2h after using 1 moxa stick and 1.5 moxa sticks respectively for fumigation and disinfection (P>0.05). There was a significant difference in the sterilization rate of air bacteria between 1 moxa stick group and 0.5 moxa stick group 0 h after air disinfection (P < 0. 05). There was a significant difference in the sterilization rate of air bacteria between 0 hour and 1 hour after burning fumigation for air disinfection in 0.5 moxa group (P < 0. 05). Conclusion — The best dose of moxa burning fumigation for air disinfection in 54m3 Chinese medicine clinic is 0.5 stick; the best time for air disinfection is 1h after disinfection.