Estrous Cycle Response in Mice (Mus musculus) with CSE (Crude Sperm Extract) Injected Intraperitoneally

The research was conducted to determine the effect of insulin-like growth factor-I (IGF-I) derived from pregnant crossbred mare serum (PMS) on estrous cycle and litter size in mice (Mus musculus). The research was arranged by Completely Randomized Design (CRD) with seven treatments and five replications. The treatments were control (CO)= physiological NaCl, treatment (T) 1 = 10 ng/ml of IGF-I pregnant mares crossbred serum (PMS), T2 = 20 ng/ml of IGF-I PMS, T3 = 40 ng/ml of IGF-I PMS, T4 = 10 ng/ml of IGF-I recombinant mouse, T5 = 20 ng/ml of IGF-I recombinant mouse, and T6 = 40 ng/ml of IGF-I recombinant mouse. Serie I the subject of this research were 35 female mice. Observed variables included estrous cycle phases that proestrus, estrus, metestrus, and diestrus phase. Serie II for the objects of this research were 35 female mice and 21 male mice in the same treatment on litter size in mice. The data were analyzed by Analysis of Variance (ANOVA), followed by HSD (Honestly Significant Difference) test. The result showed that the addition of IGF-I PMS did not significantly affect (p>0.05) on proestrus, metestrus, and diestrus phase. The addition of IGF-I PMS 40 ng/ml was extended (p<0.05) the estrus phase. It showed that there was not significantly difference (p>0.05) between the effect of IGF-I PMS and IGF-I recombinant mouse with the mice estrous cycles. The result of IGF-I derived from PMS and IGF-I recombinant mouse on litter size in mice did not significantly difference (p>0.05). Test results shows significant differences between control and 40 ng/ml of IGF-I PMS. Keywords: IGF-I, IGF-I pregnant crossbred mare serum, estrous cycle, litter sizes, Mus musculus

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Pengaruh ekstrak daun binahong (Anredera cordifolia ) terhadap pemulihan siklus estrus mencit (Mus musculus)

Ovozoa Journal of Animal Reproduction ◽

10.20473/ovz.v10i2.2021.32-38 ◽

2021 ◽

Vol 10 (2) ◽

pp. 32

Author(s):

Diana Novita Sari ◽

Ramadhan Sumarmin ◽

Fitri Arsih ◽

Fitra Arya Dwi Nugraha

Keyword(s):

Estrous Cycle ◽

Analysis Of Variance ◽

Mus Musculus ◽

Leaf Extract ◽

Positive Control ◽

Negative Control ◽

Bark Extract ◽

Vaginal Smear ◽

Level Of Significance ◽

Range Test

This study aimed to determine the effect of binahong (Anredera cordifolia) leaf extract on the recovery of the estrous cycle of mice (Mus musculus) which has been induced for the extension of the estrous cycle using angsana (Pterocarpus indicus) bark extract. In this study vaginal smear was performed daily at 10.00 WIB to determine the phase of the estrous cycle for 26 days. Mice were divided into 5 groups, each with 6 replicates. The treatments were T1 (positive control), T2 (negative control), T3 (0.8 mg/head/day), T4 (2.4 mg/head/day) and T5 (4 mg/head/day). The data obtained were processed using Analysis of Variance (ANOVA) and followed by the Duncan's New Multiple Range Test (DNMRT) at a 95% level of significance. The results showed that the angsana (Pterocarpus indicus) bark extract adminsitration (T2) lengthen (p <0.05) the estrous cycle of mice compared to those of normal mice (T1). The administration of binahong (Anredera cordifolia) leaf extract on T3 and T4 groups mice were followed by shorter (p <0.05) estrous cycle than those of T2, and it were not significantly different (p >0.05) compared to T1 group. However, the higher dose of binahong (Anredera cordifolia) leaf extract (T5) revealed the length of estrous cycle was not significantly different (p >0.05) compared to T2 group. It can be concluded that the administration of binahong leaf extract was able to recover the normal estrous cycle of mice.

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Effect of water decoction of Langsat bark (Lansium domesticum Corr.) on estrous cycle and uterus weight in mice (Mus musculus L.)

10.1063/1.4983414 ◽

2017 ◽

Author(s):

Reni Kurniati ◽

Herviana Dewi ◽

Medi Hendra

Keyword(s):

Estrous Cycle ◽

Mus Musculus ◽

Effect Of Water ◽

Lansium Domesticum

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Perubahan Kadar Estradiol dan Histologi Uterus Mencit (Mus musculus) Betina dengan Induksi Progesteron Sintetik

Biosfera ◽

10.20884/1.mib.2017.34.3.487 ◽

2017 ◽

Vol 34 (3) ◽

pp. 117

Author(s):

Erlia Narulita ◽

Jekti Prihatin ◽

Khoirul Anam ◽

Fikri Ainur Risma Hardiyanti Oktavia

Keyword(s):

Estrous Cycle ◽

Birth Control ◽

Mus Musculus ◽

Endometrial Thickness ◽

Feedback Mechanism ◽

Treatment Groups ◽

Negative Feedback Mechanism ◽

Birth Control Pills ◽

Estrogen Effect ◽

Estradiol Levels

The purpose of this research was to determine the effect of progesterone to estradiol levels and histology of uterus, to test the effect of estrous cycle to uterus histology of females’ mice after treated by progesterone in birth control pills. This research used Completely Randomized Design (CRD). Females Mus musculus Balb-C and synthetic progesterone (cyproterone acetate) contained in ‘Diane-35’ birth control pills were used in this study. The research consisted of two treatment groups, i.e. group K had no treatment and group P treated by a solution of Diane-35 at a dose of 2.6 mg per 5 mL distilled water. Results on the effects of progesterone on endometrial thickness suggested that there were significant differences between treatment groups. Meanwhile, the effect of progesterone on estradiol levels indicated that there were no significant differences among treatment groups. This means that the thickness of the endometrium was strongly influenced by the presence of a synthetic progesterone hormone and induced endometrium growth thicker. The levels of estradiol are not significantly different due to a negative feedback mechanism of Follicle Stimulating Hormone (estrogen effect) and Luteinizing Hormone (progesterone effect). The greatest thickness of endometrium of estrous cycle was treated by progesterone, which was 0.2500 mm2.

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Estrous Cycle of Mice (Mus Musculus L.) Exposed by Repeated Gamma Rays Radiation

International Journal of Pure & Applied Bioscience ◽

10.18782/2320-7051.2272 ◽

2016 ◽

Vol 4 (3) ◽

pp. 1-4

Author(s):

Ni Wayan Sudatri ◽

Keyword(s):

Estrous Cycle ◽

Gamma Rays ◽

Mus Musculus

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Intra-female aggression in the mouse (Mus musculus domesticus) is linked to the estrous cycle regularity but not to ovulation

Aggressive Behavior ◽

10.1002/ab.20217 ◽

2007 ◽

Vol 34 (1) ◽

pp. 46-50 ◽

Cited By ~ 7

Author(s):

Lorenzo Morè

Keyword(s):

Estrous Cycle ◽

Mus Musculus ◽

Female Aggression ◽

Mus Musculus Domesticus

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Zwei Fallberichte einer disseminierten Toxoplasmose

Tierärztliche Praxis Ausgabe K Kleintiere / Heimtiere ◽

10.1055/s-0038-1623622 ◽

2012 ◽

Vol 40 (01) ◽

pp. 64-69

Author(s):

C. Kiesow ◽

C. Ellenberger ◽

B. Stief

Keyword(s):

Mus Musculus ◽

Ailurus Fulgens

ZusammenfassungEs werden die Fälle einer disseminierten letalen Toxoplasmose bei einer Farbmaus (Mus musculus) und einem Roten Panda (Ailurus fulgens) vorgestellt. Es handelte sich um eine als Haustier gehaltene Farbmaus und einen Roten Panda aus einem sächsischen zoologischen Garten. Die pathologische Untersuchung ergab bei beiden Tieren eine systemische Toxoplasmeninfektion. Eine hochgradige nekrotisierende Hepatitis stellte in beiden Fällen den histologischen Hauptbefund dar. Parasitenzysten fanden sich massenhaft in der Leber, in mäßiger Zahl im Gehirn und in geringer Zahl in anderen Organen. Mittels PAS-Reaktion waren diese Zysten bei der Farbmaus kaum darstellbar, beim Roten Panda dagegen sehr deutlich. PCR bzw. Immunhistologie bestätigten die Diagnose.

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