scholarly journals Perubahan Kadar Estradiol dan Histologi Uterus Mencit (Mus musculus) Betina dengan Induksi Progesteron Sintetik

Biosfera ◽  
2017 ◽  
Vol 34 (3) ◽  
pp. 117
Author(s):  
Erlia Narulita ◽  
Jekti Prihatin ◽  
Khoirul Anam ◽  
Fikri Ainur Risma Hardiyanti Oktavia
Keyword(s):  
Estrous Cycle ◽  
Birth Control ◽  
Mus Musculus ◽  
Endometrial Thickness ◽  
Feedback Mechanism ◽  
Treatment Groups ◽  
Negative Feedback Mechanism ◽  
Birth Control Pills ◽  
Estrogen Effect ◽  
Estradiol Levels

The purpose of this research was to determine the effect of progesterone to estradiol levels and histology of uterus, to test the effect of estrous cycle to uterus histology of females’ mice after treated by progesterone in birth control pills. This research used Completely Randomized Design (CRD). Females Mus musculus Balb-C and synthetic progesterone (cyproterone acetate) contained in ‘Diane-35’ birth control pills were used in this study. The research consisted of two treatment groups, i.e. group K had no treatment and group P treated by a solution of Diane-35 at a dose of 2.6 mg per 5 mL distilled water. Results on the effects of progesterone on endometrial thickness suggested that there were significant differences between treatment groups. Meanwhile, the effect of progesterone on estradiol levels indicated that there were no significant differences among treatment groups. This means that the thickness of the endometrium was strongly influenced by the presence of a synthetic progesterone hormone and induced endometrium growth thicker. The levels of estradiol are not significantly different due to a negative feedback mechanism of Follicle Stimulating Hormone (estrogen effect) and Luteinizing Hormone (progesterone effect). The greatest thickness of endometrium of estrous cycle was treated by progesterone, which was 0.2500 mm2.

scholarly journals The use of different 17β-estradiol hormone doses and water temperatures to control cannibalism in catfish Clarias gariepinus seed

2020 ◽  
Vol 19 (2) ◽  
pp. 171-180
Author(s):  
Hylda Khairah Putri ◽  
Muhammad Zairin Jr. ◽  
Odang Carman ◽  
Iis Diatin
Keyword(s):  
Steroid Hormone ◽  
Clarias Gariepinus ◽  
Feedback Mechanism ◽  
Gonad Maturation ◽  
Negative Feedback Mechanism ◽  
Two Factors ◽  
17Β Estradiol ◽  
High Level ◽  
Estradiol 17Β ◽  
Estradiol Levels

Cannibalism is a major problem in the intensive catfish hatchery that caused high mortality. This phenomenon is allegedly due to the high level of testosterone hormones in the early larvae and seed stages. Testosterone is a maternal steroid hormone that is transferred directly by the parent to the egg. Catfish broodstock has high testosterone levels during the gonad maturation phase and it enters the eggs during the process of vitellogenesis. A high level of testosterone is considered to cause catfish seeds to behave aggressively and subsequently encourage cannibalism. This testosterone level may be reduced by estrogen through a negative feedback mechanism. This experiment aimed to evaluate the use of several 17β-estradiol doses at different water temperatures to control cannibalism in catfish seeds. This experiment used two factors, i.e. 17β-estradiol doses (0, 20, and 50 mg/kg) coated in the diet and water temperatures (28 and 31°C). The results showed that 17β-estradiol levels in catfish seeds increased with increasing experimental length. The use of 17β-estradiol at low water temperature (28°C) was better in decreasing mortality, while the dose of 50 mg/kg17β-estradiol which applied at 28°C was the best combination in controlling cannibalism on catfish seeds.   Keywords: 17β-estradiol, cannibalism, Clarias gariepinus, seed.   ABSTRAK   Kanibalisme merupakan salah satu masalah utama dalam pembenihan ikan lele intensif karena menyebabkan kematian yang tinggi. Fenomena ini diduga karena kadar hormon testosteron yang tinggi pada tahap larva dan benih. Testosteron merupakan hormon steroid maternal yang ditransfer secara langsung oleh induk ke telur. Induk ikan lele memiliki kadar testosteron yang tinggi pada fase pematangan gonad dan masuk ke dalam telur selama proses vitelogenesis. Tingginya kadar testosteron diduga menyebabkan benih berperilaku agresif dan akan mendorong kanibalisme. Kadar testosteron dapat ditekan dengan meningkatkan kadar hormon estrogen melalui mekanisme feedback negatif. Penelitian ini bertujuan mengevaluasi penggunaan dosis estradiol-17β dan suhu pemeliharaan yang berbeda untuk mengendalikan kanibalisme pada benih ikan lele. Penelitian ini menggunakan dua faktor yaitu dosis estradiol-17β yang berbeda (0, 20, dan 50 mg/kg) yang diberikan melalui pakan, dan suhu pemeliharaan yang berbeda (28 dan 31°C). Hasil penelitian menunjukkan bahwa konsentrasi estradiol-17β pada benih ikan lele meningkat seiring dengan lamanya pemeliharaan. Penggunaan estradiol-17β pada suhu 28°C lebih baik dalam mengurangi mortalitas, sementara dosis estradiol-17β 50 mg/kg pada suhu pemeliharaan 28°C adalah kombinasi terbaik dalam mengendalikan kanibalisme pada benih ikan lele.   Kata kunci: benih, Clarias gariepinus, estradiol-17β, kanibalisme.

Ultrastructural morphology of nontumorous lactotrophs in human pituitaries exposed to high prolactin levels

1987 ◽  
Vol 45 ◽  
pp. 896-897
Author(s):  
S. Jalalah ◽  
K. Kovacs ◽  
E. Horvath
Keyword(s):  
Anterior Pituitary ◽  
Secretory Activity ◽  
Pituitary Hormones ◽  
Feedback Mechanism ◽  
Endocrine Activity ◽  
Hormone Synthesis ◽  
Anterior Pituitary Hormones ◽  
Negative Feedback Mechanism ◽  
Ultrastructural Morphology ◽  
Transmission Electron

Lactotrophs, as many other endocrine cells, change their morphology in response to factors influencing their secretory activity. Secretion of prolactin (PRL) from lactotrophs, like that of other anterior pituitary hormones, is under the control of the hypothalamus. Unlike most anterior pituitary hormones, PRL has no apparent target gland which could modulate the endocrine activity of lactotrophs. It is generally agreed that PRL regulates its own release from lactotrophs via the short loop negative feedback mechanism exerted at the level of the hypothalamus or the pituitary. Accordingly, ultrastructural morphology of lactotrophs is not constant; it is changing in response to high PRL levels showing signs of suppressed hormone synthesis and secretion.By transmission electron microscopy and morphometry, we have studied the morphology of lactotrophs in nontumorous (NT) portions of 7 human pituitaries containing PRL-secreting adenoma; these lactotrophs were exposed to abnormally high PRL levels.

TERATOGENIK EKSTRAK ETANOL UWI BANGGAI UNGU (Dioscorea alata L.) PADA MENCIT BETINA (Mus musculus)

2020 ◽  
Vol 5 (2) ◽  
pp. 309-318
Author(s):  
Ihwan Ihwan ◽  
◽  
Rahmatia Rahmatia ◽  
Khildah Khaerati ◽  
Keyword(s):  
Treatment Group ◽  
Embryo Development ◽  
Mus Musculus ◽  
Birth Defect ◽  
Abnormal Development ◽  
Dioscorea Alata ◽  
Treatment Groups ◽  
Study Results ◽  
Pregnant Mice ◽  
Ethanol Extracts

Teratogenic is an abnormal development on embryo and is the cause of congenital defect or birth defect. This study aims to determine the effect of the addition of Dioscorea alata L. ethanol extracts to the embryo development on pregnant mice whose given orally to 24 mice which divided to 4 treatment groups, they are the normal group (NG) with NaCMC 0.5%; 28 mg/KgBB treatment group; 35 mg/KgBB; 42 mg/Kg BB. The addition of Dioscorea alata L ethanol extracts was done on the sixth day until the 15th day of pregnancy. On the 18th day of pregnancy, Laparaktomi was done to the pregnant mice and the embryo was taken out of the uterus. The observation was done to the fetus numbers, weight weighing of the fetus's body, dan length measurement of the fetus's body. Another observation is the observation of the external organ defect of the embryo. The study results that the addition of Dioscorea alata L ethanol extracts with various doses have no significant effect (P>0.5) to the mice external fetus development. On the examination of the fetus, we can conclude that Dioscorea alata L ethanol extracts don’t give any effect that may cause the defect of the fetus’ external organ.

Interactions Between Carbamazepine and Birth Control Pills

1989 ◽  
Vol 30 (4) ◽  
pp. 462-464 ◽  
Author(s):  
Daniel J. Rapport ◽  
Joseph R. Calabrese
Keyword(s):  
Birth Control ◽  
Birth Control Pills

scholarly journals Regulation of Store-Operated Ca2+ Entry by SARAF

Cells ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1887
Author(s):  
Inbal Dagan ◽  
Raz Palty
Keyword(s):  
Molecular Mechanisms ◽  
Feedback Mechanism ◽  
Cellular Biology ◽  
Excitable Cells ◽  
Major Pathway ◽  
Negative Feedback Mechanism ◽  
Crac Channels ◽  
Dependent Inactivation ◽  
Crac Channel ◽  
The One

Calcium (Ca2+) signaling plays a dichotomous role in cellular biology, controlling cell survival and proliferation on the one hand and cellular toxicity and cell death on the other. Store-operated Ca2+ entry (SOCE) by CRAC channels represents a major pathway for Ca2+ entry in non-excitable cells. The CRAC channel has two key components, the endoplasmic reticulum Ca2+ sensor stromal interaction molecule (STIM) and the plasma-membrane Ca2+ channel Orai. Physical coupling between STIM and Orai opens the CRAC channel and the resulting Ca2+ flux is regulated by a negative feedback mechanism of slow Ca2+ dependent inactivation (SCDI). The identification of the SOCE-associated regulatory factor (SARAF) and investigations of its role in SCDI have led to new functional and molecular insights into how SOCE is controlled. In this review, we provide an overview of the functional and molecular mechanisms underlying SCDI and discuss how the interaction between SARAF, STIM1, and Orai1 shapes Ca2+ signaling in cells.

scholarly journals Direct observation of a coil-to-helix contraction triggered by vinculin binding to talin

2020 ◽  
Vol 6 (21) ◽  
pp. eaaz4707 ◽  
Author(s):  
Rafael Tapia-Rojo ◽  
Alvaro Alonso-Caballero ◽  
Julio M. Fernandez
Keyword(s):  
Focal Adhesions ◽  
Interaction Force ◽  
Magnetic Tweezers ◽  
Feedback Mechanism ◽  
Force Transmission ◽  
Mechanical Coupling ◽  
Negative Feedback Mechanism ◽  
The Reformation ◽  
Energy Penalty ◽  
First Time

Vinculin binds unfolded talin domains in focal adhesions, which recruits actin filaments to reinforce the mechanical coupling of this organelle. However, it remains unknown how this interaction is regulated and its impact on the force transmission properties of this mechanotransduction pathway. Here, we use magnetic tweezers to measure the interaction between vinculin head and the talin R3 domain under physiological forces. For the first time, we resolve individual binding events as a short contraction of the unfolded talin polypeptide caused by the reformation of the vinculin-binding site helices, which dictates a biphasic mechanism that regulates this interaction. Force favors vinculin binding by unfolding talin and exposing the vinculin-binding sites; however, the coil-to-helix contraction introduces an energy penalty that increases with force, defining an optimal binding regime. This mechanism implies that the talin-vinculin-actin association could operate as a negative feedback mechanism to stabilize force on focal adhesions.

Dynamics of oxytocin, estrogen and progestin receptors in the bovine endometrium during the estrous cycle

1988 ◽  
Vol 118 (1) ◽  
pp. 96-104 ◽  
Author(s):  
H. H. D. Meyer ◽  
Th. Mittermeier ◽  
D. Schams
Keyword(s):  
Estrous Cycle ◽  
Luteal Phase ◽  
Oxytocin Receptor ◽  
Minor Importance ◽  
Progestin Receptors ◽  
Progestin Receptor ◽  
Nuclear Estrogen Receptor ◽  
Low Sensitivity ◽  
A Minor ◽  
Estradiol Levels

Abstract. The levels of oxytocin receptor (OTR), cytosolic progestin receptor (cPR), cytosolic and nuclear estrogen receptor (cER, nER) were measured in the endometrium of 28 heifers that had been slaughtered on a defined day of the estrous cycle, In an additional, trial endometrial tissue obtained from 78 heifers or cows at the abattoir was analyed for OTR. OTR was absent during the luteal phase (after day 6), but a minor elevation was observed after day 15. OTR increased rapidly after luteolysis on days 17–18 reaching a maximum during estrous on day 21, and decreased again during days 1–6. cER and cPR were different to OTR but followed a similar pattern with maximal levels during days 1–8 of the estrous cycle. At day 12 both receptors were minimal and increased again towards day 21. nER was maximal at day 19–21 coinciding with maximal estradiol levels and estrous. Our data indicate that owing to an increasing sensitivity of the endometrium to progesterone and estradiol after day 12, these steroids may be mainly responsible for the initiation of first PGF2α surges and luteolysis. Oxytocin seems to be of minor importance at this stage owing to low sensitivity of the endometrium for oxytocin.

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