Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia

Abstract. Khotimah S, Suharjono, Ardyati T, Nurani Y. 2020. Isolation and identification of cellulolytic bacteria at fibric, hemic, and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia. Biodiversitas 21: 2103-2112. Cellulose degrading bacteria was one of the microbial removers of organic matter contained in the soil into simpler monomers so that it can be utilized by other organisms. The objective of the research was to obtain cellulose-degrading bacteria found on fibric, hemic, and sapric peat in forest and shrubs (oil palm). The bacteria were isolated by pour plate method on 1% CMC media. Selected isolates were assayed quantitatively based on the activity of cellulase enzyme, identified with 16S rDNA. The density of cellulolytic bacteria in the secondary forest peat of fibric, hemic, sapric were 2.1x103 cfu/g, 5.9x104 cfu/g, and 4.9x104 cfu/g whereas, in the area of shrubs/oil palm peat fibric, hemic and sapric 6.9x104 cfu/g, 8.4x104 cfu/g and 3.4x105 cfu/g respectively. There were 19 bacterial isolates that have clear zones around the colony as degradation of cellulose had highest ability to degrade cellulose with clear zones of 5-7 mm. The strain of SB1.1.1 showed highest activity of cellulase enzyme 11.17 U/mL, followed by HH3.1.1 strain and SB2.3 7.83 U/mL. Based on the phylogeny tree, strain SB1.1.1 and HH3.1.1 have the closest kinship relationship with Bacillus cereus with a kinship relationship of 100%, while SB2.3 has the closest kinship relationship with Bacillus stratosphericus with a relationship of 99.85 %.

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Isolation and Identification of Indigenous Lactic Acid Bacteria by Sequencing the 16S rRNA from Dangke, A Traditional Cheese from Enrekang, South Sulawesi

Pakistan Journal of Nutrition ◽

10.3923/pjn.2017.384.392 ◽

2017 ◽

Vol 16 (5) ◽

pp. 384-392 ◽

Cited By ~ 3

Author(s):

Setiawan Putra Syah ◽

Cece Sumantri ◽

Irma Isnafia Arief ◽

Epi Taufik

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

16S Rrna ◽

Isolation And Identification ◽

South Sulawesi ◽

Traditional Cheese

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Isolation and Identification Cellulolytic Bacteria from Termite Gut Obtained from Indralaya Peatland area

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/926/1/012024 ◽

2021 ◽

Vol 926 (1) ◽

pp. 012024

Author(s):

D Oktiarni ◽

Hermansyah ◽

Hasanudin ◽

Miksusanti ◽

E Nofyan ◽

...

Keyword(s):

Alternative Energy ◽

Organic Waste ◽

Agar Medium ◽

Agricultural Waste ◽

Cellulolytic Bacteria ◽

Determinative Bacteriology ◽

Isolation And Identification ◽

Promising Alternative ◽

Second Generation Bioethanol ◽

Termite Gut

Abstract The production of second-generation bioethanol as renewable energy has developed very rapidly and has become a promising alternative energy source. Bioethanol production using biomass can be obtained alternatively from cellulose in wood, sawdust, organic waste, and agricultural waste. This research used termites obtained from Indralaya peatland area as organisms that can decompose cellulose into glucose with the cellulase enzymes produced by bacteria in their digestive tract. Cellulases are enzymes capable of hydrolyzing lignocellulose into glucose. The study aimed to isolate and identify of cellulolytic bacteria from termite gut obtained from Indralaya peatland area. The bacterial isolates were classified by using morphological and biochemical standard methods, and identification based on Bergey’s Manual of Determinative Bacteriology. Cellulolytic bacteria of termite gut were isolated and cultured on CMC (Carboxymethyl cellulose) agar medium. The activity of cellulolytic bacterial was conducted based on halo area and cellulolytic index on CMC agar medium. Among 64 isolates of bacteria, 24 isolates were identified as cellulolytic bacteria. Futhermore, our isolates with higher cellulolytic index were identified as the Staphylococcus, Microbacterium, Bacillus, and Brevibacterium genus.

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Isolation and Identification of Native Mikoriza Morphology on The Rhizosphere Gluta rengas L. in Jompie Botanical Garden

AgroTech Journal ◽

10.31327/atj.v4i2.1094 ◽

2019 ◽

Vol 4 (2) ◽

pp. 69-74

Author(s):

Muhammad Ilham ◽

Andi Adam Malik ◽

A. Amri ◽

Muhammad Akhsan Akib

Keyword(s):

Research And Development ◽

Botanical Garden ◽

Plant Roots ◽

Microbiology Laboratory ◽

Isolation And Identification ◽

Development Environment ◽

South Sulawesi ◽

High Level ◽

The City ◽

Glomus Sp

Alitta Forest, located in the city of Parepare, South Sulawesi, has an area of 84 ha, a portion of this forest area is functioned as part of a botanical garden. The jompie botanical garden has an area of 13.5 ha, with a collection of plants reaching 90 species originating from 81 plant clans and as many as 77 species that have been identified. In addition to a collection of high-level plants, jompie botanical gardens also have a diversity of microorganisms that have not been identified, especially microorganisms that symbiosis with plant roots known as mycorrhiza, so the purpose of this study is to identify and identify the abundance of mycorrhizal spores in the jompie botanical garden found in rhizosphere Gluta renghas L.. The research began with taking the rhizosphere under the stands of wet trees in the jompie botanical garden, which was then continued to identify and calculate the abundance of spores in the microbiology laboratory of Makassar's research and development environment and forestry. The identification results of mikoiza spores native to the jompie botanical garden show that they are found in two genera, namely; Acalauspora sp consisting of two morphotypes, and the genus Glomus sp consisting of one morphotype, with an average spore abundance of 45.3 per 100 grams rhizosphere

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Isolation and Identification of Cellulose Degrading Bacteria from Banana Peel Compost

el–Hayah ◽

10.18860/elha.v7i1.7241 ◽

2019 ◽

Vol 7 (1) ◽

pp. 6-11

Author(s):

Yendania Grevitara P. ◽

Badriyatur Rahma F. ◽

Hellen Septirangga P. ◽

Irma Dahlia Y. ◽

Endang Suarsini

Keyword(s):

Burkholderia Cepacia ◽

Cellulose Degradation ◽

Complex Compounds ◽

Banana Peel ◽

Identification System ◽

Cellulolytic Activity ◽

Cellulolytic Bacteria ◽

Isolation And Identification ◽

Degrading Bacteria

Cellulolytic bacteria are bacteria that have the ability to hydrolyze cellulose complexes into smaller oligosaccharides and eventually become glucose. Glucose is used as a carbon and energy source for bacterial growth. This study was conducted to isolate the cellulose degrading bacteria from banana peel compost that produce cellulose enzymes based on the clear zone that visible around the colony. The cellulolytic activity was determined by the ability of bacteria to hydrolyze the Carboxymethyl Cellulose (CMC) substrate. Determination of cellulolytic activity is known based on cellulolytic index calculation, the diameter total minus the diameter of the colony and divided by the diameter of the colony. The result of five bacterial isolates was found but only one bacterium had the potential to be a cellulose degradation. Based on the Microbact Gram-Negative Identification System, the bacterium is Burkholderia cepacia. These bacteria have an important role in nature as decomposers of various complex compounds, such as cellulose, hemicellulose, lignin, and pectin.

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