Screening of Antimicrobial and Antioxidant Activities of Moringa oleifera Leaf Extracts Against Multidrug Resistant Pathogenic Bacteria

2020 ◽  
Vol 13 (3) ◽  
pp. 1021-1030
Author(s):  
Wesal Waheeb Khouj
Keyword(s):  
Pathogenic Bacteria ◽  
Moringa Oleifera ◽  
Antioxidant Activities ◽  
Multidrug Resistant ◽  
Leaf Extracts

scholarly journals Screening of multidrug-resistant bacteria of antibiotic-associated diarrhea and their inhibition by leaf extracts of Moringa oleifera Lamk

2014 ◽  
Vol 20 ◽  
pp. 41-48
Author(s):  
MM Akhtar ◽  
MS Islam ◽  
MF Begum ◽  
M Anisuzzaman ◽  
MF Alam
Keyword(s):  
Petroleum Ether ◽  
Moringa Oleifera ◽  
Multidrug Resistant ◽  
Inhibitory Effect ◽  
Hot Water ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Leaf Extracts ◽  
Multidrug Resistant Bacteria ◽  
Fresh Leaf

Context: Emergence of multi drug resistance bacteria (MDRB) to human pathogenic infection is increasing day by day but the number of new drugs to overwhelm the problem is not sufficient. Evidences revealed that Moringa oleifera Lamk. has various pharmaceutical activities like antibacterial, antifungal, antispasmodic, anti-inflammatory, anticancer and diuretic. Herbal treatment may be one of the possible ways to treat diseases caused by multidrug-resistant bacteria. Objectives: The present research was undertaken to screen of multidrug resistant bacteria (MDRB) from antibiotic-associated diarrheal samples and to evaluate the potentiality of M. oleifera leaf extracts on these bacteria with the view to provide scientific evidence for its application in health remedy. Materials & Methods: Antibiotic-associated diarrheal fecal specimens were collected from pediatric ward of Rajshahi Medical College and cultured onto MacConkey agar. MDRB were determined by antibiotic susceptibility test, using disc-agar diffusion method. Biochemical tests of the MDRB were done according to Bergey’s Manual of Determinative Bacteriology for identification of the species. Dried and fresh leaf of M. oleifera was used to prepare exaction with or without solvents such as hot water, cold water, chloroform, petroleum ether, acetone and ethanol, separately. Antibacterial assay was done by disc diffusion method and minimum inhibitory concentration of the extracts was also measured. Results: In the present study seven isolates were screened as MDRB and the highest prevalence (42.86%) was occurred in the age group of 25-36 months and the lowest (14.28) was in the group of <1 and 1-6 months. Ethanol extract of dried leaf of M. oleifera Lamk. showed moderate inhibitory activity against all of the isolates while petroleum ether, chloroform and acetone extracts of dried leaf have no inhibitory effect. Fresh leaf sap powder in DMSO exhibited strong inhibitory effect against all of the test bacteria where as hot aqueous extract could not show any inhibition. The minimum inhibitory concentrations (MIC) of the potent extracts ranged 937.5 to 3750 ?g/ml and 7.9 to 234.4 ?g/ml in dried and fresh leaf extracts, respectively. Conclusion: The present data indicates that M. oleifera leaf extract possess antimicrobial potential to control of MDRB causes infection thus it can be used as a novel drugs in future. DOI: http://dx.doi.org/10.3329/jbs.v20i0.17652 J. bio-sci. 20: 41-48, 2012

scholarly journals Action of Fractionated Moringa oleifera Lam Leaf Extracts on Multidrug Resistant Pseudomonas aeruginosa Strains

2021 ◽  
Vol 5 (3) ◽  
pp. 316-329
Author(s):  
Eremwanarue Aibuedefe Osagie ◽  
Erhauyi Osayemwenre ◽  
Nwawuba Stanley Udogadi ◽  
Shittu Hakeem Olalekan
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Moringa Oleifera ◽  
Multidrug Resistant ◽  
Leaf Extracts ◽  
Moringa Oleifera Lam

scholarly journals The interference of Moringa oleifera leaf extracts to modulate quorum sensing-facilitated virulence factors

2019 ◽  
Vol 20 (10) ◽  
Author(s):  
Suhartono Suhartono ◽  
Yulia Sari Ismail ◽  
Syarifah Raisha Muhayya
Keyword(s):  
Quorum Sensing ◽  
Virulence Factors ◽  
Bacterial Infections ◽  
Pathogenic Bacteria ◽  
Moringa Oleifera ◽  
Bioactive Constituents ◽  
Leaf Extracts ◽  
Antibiotic Resistant ◽  
Potent Inhibition ◽  
Ethanolic Extracts

Abstract. Suhartono S, Ismail YS, Muhayya SR. 2019. The interference of Moringa oleifera leaf extracts to modulate quorum sensing-facilitated virulence factors. Biodiversitas 20: 3000-3004. The infections attenuated by the presence of virulence factors might be facilitated by quorum sensing (QS) mechanism. The continuous treatments of bacterial infections with current drugs, however, has developed such pathogenic bacteria more antibiotic-resistant, encouraging to searching for better alternative treatment, such as the use of plant-derived bioactive constituents, including kelor (Moringa oleifera L.). In the present research, kelor (M. oleifera) ethanolic leaf extract was evaluated for their potency as an antibacterial agent to inhibit bacterial virulence factors mediated by quorum sensing mechanism against Chromobacterium violaceum and Pseudomonas aeruginosa PAO1. A phytochemical examination of M. oleifera leaf-ethanolic extracts has been conducted resulting positive detection of alkaloids, steroids, terpenoids, flavonoids, and phenolics or tannins. The extracts had MIC and MBC values of 10 mg/mL and 20 mg/mL, respectively, for both tested bacteria. The ethanolic extracts of M. oleifera leaves also showed potent inhibition of quorum sensing by significantly reducing violacein and pyocyanin production as well as interference of swarming motility and biofilm formation.

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