scholarly journals Immunological aspects of bacterial keratites in patients with diabetes mellitus

2021 ◽  
Vol 9 (2) ◽  
pp. 6-9
Author(s):  
O.V. Zavoloka ◽  
P.A. Bezditko ◽  
L.P. Abramova ◽  
V.O. Vekshyn
Keyword(s):  
Diabetes Mellitus ◽  
Anterior Segment ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bacterial Keratitis ◽  
Healthy Individuals ◽  
Lacrimal Fluid ◽  
Cytokine Balance ◽  
Patients With Diabetes ◽  
Contralateral Eye ◽  
Type 1 Dm

Background. The purpose was to analyze the cytokine balance of lacrimal fluid in patients with bacterial keratitis and diabetes mellitus (DM) at the first visit and to identify the immunological aspects of the disease. Materials and methods. The analysis of pro- and anti-inflammatory cytokine concentration in the lacrimal fluid was performed in 17 patients with type 1 DM and bacterial keratitis and 15 nondiabetic patients with bacterial keratitis at the first visit. Data from 14 healthy individuals were used for comparison. In addition to standard ones, ophthalmic examination methods included bacteriological examination, fluorescein test, anterior segment optical coherence tomography, non-contact corneal aesthesiometry. The levels of interleukin (IL) 1β, IL-6 and IL-10 in the lacrimal fluid of the sick and the contralateral eye were determined by a quantitative colorimetric enzyme-linked immunosorbent assay using ELISA kits. Results. In DM patients with bacterial keratitis, the concentration of IL-1β and IL-6 in the lacrimal fluid of the sick eye exceeded that in healthy individuals (p < 0.05) and did not differ significantly from nondiabetic patients with bacterial keratitis (p > 0.05). In the lacrimal fluid of the contralateral eye of DM patients with bacterial keratitis, the level of IL-1β and IL-6 exceeded the corresponding indicators of nondiabetic patients with bacterial keratitis and healthy individuals (p < 0.05). The concentration of IL-10 in the lacrimal fluid of the contralateral eye in DM patients with bacterial keratitis exceeded that in healthy individuals (p < 0.05) and did not significantly differ from those in nondiabetic patients with bacterial keratitis (p > 0.05). Conclusions. DM patients with bacterial keratitis have immunological features of the disease.

IMMUNOLOGICAL FEATURES OF THE DYNAMICS OF THE I DEGREE SEVERETY BACTERIAL KERATITES DEPENDING ON THE PRESENCE OF DIABETES MELLITUS

2021 ◽  
pp. 63-68
Author(s):  
O. V. Zavoloka ◽  
P. A. Bezditko ◽  
L. P. Abramova ◽  
V. O. Vekshyn
Keyword(s):  
Diabetes Mellitus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Observation Data ◽  
Bacterial Keratitis ◽  
Lacrimal Fluid ◽  
Patients With Diabetes ◽  
Contralateral Eye ◽  
Instructions For Use ◽  
Type 1 Dm

Summary. In patients with diabetes mellitus (DM) there are features of the dynamics of the I degree severity bacterial keratitis, leading to prolongation of the disease and worsening of its consequences. The aim was to identify immunological features of the dynamics of the I degree severity bacterial keratitis depending on the presence of type 1 DM. Materials and methods. The level of interleukins (IL) was analyzed, namely IL-1β, IL-6 and IL-10 in the lacrimal fluid of the sick and the contralateral eye of 6 patients with type 1 DM and the I degree severity bacterial keratitis and 6 nondiabetic patients with I degree severity bacterial keratitis on the 1st day, 10-14th day and 24th day of observation. Data from 14 healthy individuals of the appropriate age were used for comparison. The severity of bacterial keratitis was determined according to our scheme at a total score of ≤ 14. All patients were treated with instillations of ofloxacin, antiseptics, antioxidants, reparents, artificial tears, mydriatics, systemic anti-inflammatory therapy. The level of IL in the lacrimal fluid was determined by quantitative colorimetric enzyme-linked immunosorbent assay using ELISA kits by ANOGEN according to the instructions for use. Results and discussion. In patients with I degree severity bacterial keratitis, both DM and nondiabetic, on the 1st day of observation there was an increase in the level of IL-1β and IL-6 in the lacrimal fluid of the sick eye and IL-10 – of the contralateral eye compared to healthy persons, on the 10th-14th day — increase in the level of IL-1β and IL-6 of the sick and the contralateral eye, as well as IL-10 – of the contralateral eye (p<0.05). On the 24th day in nondiabetic patients with I degree severity bacterial keratitis normalization of interleukin levels in the lacrimal fluid of the sick and the contralateral eye was found, but in DM patients remained elevated levels of IL-1β, IL-6, both in the sick and in the contralateral eye (p<0.05). Conclusions. In DM patients there are immunological features of the dynamics of the I degree severity bacterial keratitis, namely a longer increase in the level of proinflammatory cytokines IL-1β, IL-6 in the lacrimal fluid of the sick and the contralateral eye.

scholarly journals PECULIARITIES OF THE LACRIMAL FLUID CYTOKIN PROFILE IN PATIENTS WITH BACTERIAL KERATITITIS OF THE II DEGREE SEVERETY AND DIABETES MELLITUS

2021 ◽  
pp. 61-65
Author(s):  
O. V. Zavoloka ◽  
P. A. Bezditko ◽  
L. P. Abramova ◽  
V. O. Vekshyn
Keyword(s):  
Proinflammatory Cytokines ◽  
Cytokine Profile ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bacterial Keratitis ◽  
Lacrimal Fluid ◽  
Anti Inflammatory ◽  
Contralateral Eye ◽  
Type 1 Dm ◽  
Degree Of Severity

Summary. The aim was to identify the features of the lacrimal fluid cytokine profile in patients with bacterial keratitis of the II degree of severity and type 1 DM in the dynamics of observation. Materials and methods. The lacrimal fluid cytokine profile was analyzed in 6 patients with type 1 DM and bacterial keratitis of the II degree of severity and 6 nondiabetic patients with bacterial keratitis of the II degree of severity on the 1st day, 10-14th day and 24th day of observation. Data from 14 healthy individuals of the appropriate age were used for comparison. II degree of severity of bacterial keratitis was determined according to our proposed scheme at a sum of points 15-21. All patients were treated with instillations of ofloxacin, antiseptics, antioxidants, reparents, artificial tears, mydriatics, systemic anti-inflammatory therapy. The concentration of interleukins (IL)-1β, IL-6 and IL-10 in the lacrimal fluid of the sick and the contralateral eye was determined by quantitative colorimetric enzyme-linked immunosorbent assay using ELISA kits. Results and discussion. In patients with bacterial keratitis of the II degree of severity, both with DM and nondiabetic, within 10-14 days of observation invented the expression of proinflammatory cytokines IL-1β and IL-6 in the lacrimal fluid of the sick eye and anti-inflammatory cytokine IL-10 - in lacrimal fluid of the contralateral eye (p<0.05). In DM patients, a longer expression of IL-1β and IL-6 was detected in the lacrimal fluid of the sick and the contralateral eye, which was observed on the 24th day of the study (p<0.05). Conclusions. There are features of the lacrimal fluid cytokine profile in DM patients with bacterial keratitis of the II severity in the dynamics of observation, namely the longer expression of proinflammatory cytokines IL-1β, IL-6 in the sick and the contralateral eye.

Analysis of the Cytokine Balance in Tear Fluid of Diabetic Patients with Bacterial Keratitis Depending on the Severity of the Disease

2021 ◽  
pp. 18-25
Author(s):  
Olesia V. Zavoloka ◽  
Pavlo A. Bezditko ◽  
Liliya P. Abramova ◽  
Vitaliy O. Vekshyn
Keyword(s):  
Diabetes Mellitus ◽  
Proinflammatory Cytokines ◽  
Tear Fluid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diabetic Patients ◽  
Bacterial Keratitis ◽  
Cytokine Balance ◽  
Anti Inflammatory ◽  
Contralateral Eye ◽  
Severity Of The Disease

The aim. To analyze the cytokine balance of tear fluid in patients with bacterial keratitis at presentation depending on the severity of the disease and the presence of diabetes mellitus (DM). Materials and methods. The analysis was performed through the comparison of the level of pro- and anti-inflammatory cytokines in the tear fluid of 17 patients with type 1 DM and bacterial keratitis and 15 patients with bacterial keratitis without DM at presentation. Data from 14 healthy individuals of the appropriate age were also used for comparison. The patients with bacterial keratitis were divided into subgroups according to the severity of bacterial keratitis. The levels of IL-1β, IL-6 and IL-10 in the tear fluid of the sick and the contralateral eye were determined by quantitative colorimetric enzyme-linked immunosorbent assay. Results and discussion. At presentation, patients with bacterial keratitis, both with and without DM, showed increased levels of proinflammatory cytokines, namely IL-1β and IL-6, in the tear fluid of the sick eye, which correlated with the severity of the disease, and also increased level of anti-inflammatory cytokine IL-10 in the tear fluid of the contralateral eye. In addition, the concentrations of proinflammatory cytokines IL-1β, IL-6 in the tear fluid of the contralateral eye in DM patients were increased at all degrees of severity of bacterial keratitis. Conclusions. In patients with bacterial keratitis, the cytokine balance of the tear fluid of the sick and the contralateral eye depends on the severity of the disease and the presence of DM. Keywords: diabetes mellitus, bacterial keratitis, severity of keratitis, cytokines, interleukins.

scholarly journals FEATURES OF THE DYNAMICS OF THE II DEGREE OF SEVERETY BACTERIAL KERATITES IN DIABETIC PATIENTS AT DIFFERENT STAGES OF DIABETIC POLYNEYROPATHY

2021 ◽  
pp. 5-10
Author(s):  
O. V. Zavoloka
Keyword(s):  
Clinical Symptoms ◽  
Healing Process ◽  
Anterior Segment ◽  
Diabetic Polyneuropathy ◽  
Diabetic Patients ◽  
Bacterial Keratitis ◽  
Results Of Treatment ◽  
Patients With Diabetes ◽  
Type 1 Dm ◽  
Degree Of Severity

Summary. The neuro-trophic condition of the cornea affects the healing process of corneal defects in patients with keratitis, causing the severity, duration and consequences of the disease. The aim was to identify the features of the dynamics of the II degree of severity bacterial keratitis in patients with diabetes mellitus (DM) at different stages of the severity of diabetic polyneuropathy (DPN). Materials and methods. The results of treatment of 19 patients with bacterial keratitis of the II degree of severity and type 1 DM were analyzed. II degree of severity of bacterial keratitis was determined according to our proposed scheme at a sum of points 15-21. All patients were treated with instillations of the antibiotic ofloxacin, antiseptics, antioxidants, reparants, artificial tears, mydriatics, systemic anti-inflammatory therapy. Patients were divided into two groups according to the severity of DPN: with symptomatic (first group) and stage with impaired DPN (second group). In addition to standard, ophthalmic examination methods included bacteriological examination, fluorescein test, OCT of the anterior segment of the eye, non-contact corneal esthesiometry. Results and discussion. In most patients of the second group, corneal sensitivity and depth of edema of the corneal tissues surrounding the ulcer, the size of the ulcer defect and the depth of corneal infiltration, the degree of pericorneal injection, the depth of the corneal ulcer defect from the 1st, 7th, 10th and 14th days of observation, respectively, were higher than in patients of the first group (p<0,05). The consequence of the disease in patients of the second group was more severe than in patients of the first group. Conclusions. In patients with the stage of impaired DPN, bacterial keratitis is characterized by a longer duration and severity of clinical symptoms, as well as worse consequences of the disease than in patients with symptomatic DPN.

scholarly journals A literature review and meta-analysis of safety profiles of SGLT2 inhibitors in Japanese patients with diabetes mellitus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Junichi Mukai ◽  
Shinya Kanno ◽  
Rie Kubota
Keyword(s):  
Diabetes Mellitus ◽  
Meta Analysis ◽  
Japanese Patients ◽  
Sglt2 Inhibitors ◽  
Patients With Diabetes ◽  
Language Restriction ◽  
Calculated Risk ◽  
Favorable Safety ◽  
Type 1 Dm

AbstractThe safety profiles of sodium-glucose co-transporter 2 (SGLT2) inhibitors may depend on races/ethnicities. We aimed to assess the safety profiles of SGLT2 inhibitors in Japanese patients with diabetes mellitus (DM). The electronic databases MEDLINE, CENTRAL, and Ichushi-web were searched for studies with no language restriction from their inception to August 2019. Trials were included in the analysis if they were randomized controlled trials (RCTs) comparing the effects of SGLT2 inhibitors with a placebo in Japanese patients with DM > 18 years and reporting HbA1c and at least 1 adverse event. We calculated risk ratios with 95% CIs and used a random-effects model. Of the 22 RCTs included in our review, only 1 included patients with type 1 DM. The durations of RCTs ranged between 4 and 24 weeks. In comparison with a placebo, SGLT2 inhibitors were associated with similar risks of hypoglycemia, urinary tract infection, genital infection, hypovolemia, and fracture. The outcomes of treatment with SGLT2 inhibitors among Japanese patients with DM suggest favorable safety profiles. However, further evidence from studies with a longer duration, involving more diverse populations, such as patients with different types of DM, or including individual SGLT2 inhibitors is needed to resolve the limitations of the present study.

Corneal Sensitivity in Bacterial Keratitis Patients with or without Diabetes Mellitus

2021 ◽  
pp. 67-72
Author(s):  
O. V. Zavoloka
Keyword(s):  
Diabetes Mellitus ◽  
Anterior Segment ◽  
Control Group ◽  
Sensitivity Threshold ◽  
Diabetic Patients ◽  
Bacterial Keratitis ◽  
Corneal Sensitivity ◽  
Fellow Eye ◽  
Nondiabetic Control

The aim. To identify the features of corneal sensitivity of the sick and the fellow eye in bacterial keratitis patients with or without diabetes mellitus. Materials and methods. The analysis was performed on the basis of survey data of 62 type 1 diabetes patients with bacterial keratitis and 43 nondiabetic control patients with bacterial keratitis. The examination was performed at the first visit. In addition to standard ophthalmic examination, the patients underwent fluorescein test, OCT of the anterior segment of the eye, non-contact corneal aesthesiometry. Results and discussion. The average corneal sensitivity threshold in diabetic patients with bacterial keratitis at the first visit at all air flow temperatures exceeded the corresponding indicators in nondiabetic patients of the control group: by 33.5% at an air temperature of 5 °C, by 30.6% at 15 °C, by 28.8% at 20 °C, by 27% at 30 °C, and by 26.1% at 40 °C (p<0.05). The average corneal sensitivity threshold at a temperature of 20 °C in the fellow eye in diabetic patients with bacterial keratitis exceeded that in nondiabetic patients with bacterial keratitis of the control group by 32.9 % (p<0.05). Conclusions. Corneal sensitivity of the sick and fellow eye in patients with bacterial keratitis depends on the presence of diabetes mellitus. The average threshold of corneal sensitivity of the sick and fellow eye in diabetic patients with bacterial keratitis at the first visit exceeds the corresponding values in nondiabetic patients with bacterial keratitis. Keywords: diabetes mellitus, bacterial keratitis, corneal sensitivity.

scholarly journals INTERRELATION BETWEEN THE CHANGES IN QUALITY OF LIFE AND ARTERIAL STIFFNESS IN PATIENTS WITH DIABETES MELLITUS TYPE 2 AND CONCOMITANT OBESITY

2018 ◽  
Vol 18 (4) ◽  
pp. 71-75
Author(s):  
I.M. Fushtey ◽  
Ye.A. Solovyuk ◽  
A.O. Solovyuk
Keyword(s):  
Quality Of Life ◽  
Physical Activity ◽  
Diabetes Mellitus ◽  
Emotional State ◽  
Eating Behaviour ◽  
Overweight And Obesity ◽  
Healthy Individuals ◽  
Patients With Diabetes

The purpose of this work was to study the general characteristics of quality of life (QoL), the effect of overweight on QoL, the nature of eating behaviour in patients with diabetes mellitus (DM) type 2 and   concomitant overweight (OW) and obesity, as well as to establish the correlation with indicators of functional state of the arterial vessels. 64 people (34 women and 30 men) with DM and concomitant  overweight and obesity (average age 56.3 ± 10.23 years) formed the 1 group, 34 people (19 women and 15 men), whose average age was 55.6 ± 11.92 years constituted the 2 group, and  28 healthy individuals formed the control group. SF-36v2 questionnaires were used to evaluate QoL. The effect of overweight on QoL was analyzed according to the IWQOL-Lite questionnaire data, the patterns of eating behaviour were determined by the COEQ4 for 7 days using FPRS questionnaire. The functional state of the arterial vessels was assessed by estimating the pulse wave velocity using the automated rheographic complex ReoCom (KhAI Medika (Ukraine)). The patients with DM and concomitant overweight and obesity were found to experience some changes in QoL according to the SF-36v2 questionnaire. The changes were primarily characterized by a decrease in physical activity, as well as in social and emotional status. These changes differed not only from the QoL assessment by healthy individuals, but also from those of patients with DM and normal body weight. The nature of eating behaviour in overweight or obese patients with DM was characterized by an increased hunger in parallel with a worsened emotional state and an increased desire to eat certain types of foods that can contribute to weight gain. Structural changes in arterial vessels that typically are indicative of arterial stiffness correlate with indicators of emotional state and physical activity in patients with DM and comorbid overweight and obesity.

INTERRELATION OF HEMORHEOLOGICAL PARAMETERS WITH GLUCOSE AND GLYCATED HEMOGLOBIN IN PATIENTS WITH DIABETES MELLITUS TYPE2

2018 ◽  
Author(s):  
А.В. Муравьев ◽  
И.А. Тихомирова ◽  
С.В. Булаева ◽  
Ю.В. Малышева ◽  
А.В. Замышляев
Keyword(s):  
Diabetes Mellitus ◽  
Glycated Hemoglobin ◽  
Healthy Individuals ◽  
Glucose Content ◽  
Metabolic Hormones ◽  
Metabolism Regulation ◽  
Patients With Diabetes ◽  
Glycated Hemoglobin Level ◽  
Blood Transport

Введение. Нарушения реологических свойств крови при сахарном диабете 2 типа (СД-2) может приводить к снижению микрососудистой перфузии. Основной механизм, вероятно, связан с ухудшением микрореологии эритроцитов, в том числе из-за высокого содержания глюкозы и гликозилирования белков мембран клеток. Цель исследования: провести анализ связи гемореологических характеристик цельной крови и эритроцитов с содержанием глюкозы и гликированного гемоглобина и выявить влияние ряда метаболических гормонов на микрореологию эритроцитов у больных СД-2. Материалы и методы. У 30 больных СД-2 регистрировали параметры гемореологического профиля. Наряду с измерениями вязкости, деформируемости эритроцитов (ДЭ) и их агрегации (АЭ) определяли содержание глюкозы, гликированного гемоглобина и белков плазмы (альбуминов, глобулинов, фибриногена). В in vitro исследованиях регистрировали микрореологические характеристики эритроцитов после их инкубации с глюкозой и рядом метаболических гормонов (инсулином, глюкагоном, адреналином). Результаты. Наиболее существенные отличия гемореологических профилей у больных СД-2 от данных здоровых лиц были в их микрореологической части. Значения глюкозы и гликированного гемоглобина более выражено коррелировали с показателями гемореологического профиля у больных СД-2, чем у здоровых лиц: коэффициент корреляции между АЭ и концентраций глюкозы у больных СД-2 составил 0,660, в контрольной группе — 0,480. Под влиянием инсулина ДЭ достоверно повышалась, а АЭ, напротив, снижалась (p < 0,05). При инкубации эритроцитов с глюкагоном у больных СД-2 и у здоровых лиц АЭ была снижена на сходную величину. Адреналин в трех концентрациях умеренно повышал ДЭ (p < 0,05), а наблюдавшийся прирост АЭ был наибольшим при низкой концентрации гормона (0,01 мкМ). Заключение. Анализ параметров гемореологического профиля у больных СД-2 свидетельствует о нарушениях текучести крови и снижении ее транспортных возможностей. Также было показано, что гормоны, участвующие в регуляции метаболизма, оказывают прямое воздействие на микрореологию эритроцитов. Introduction. Disorders of blood rheological properties in diabetes mellitus type 2 (DM-2) can lead to reduction of microvascular perfusion. The main mechanism is probably associated with impairment of erythrocytes microrheology and also due to high glucose content and glycosylation of cell membrane proteins. Aim: to analyze the relationship between hemorheological characteristics of whole blood and erythrocytes with glucose content and glycated hemoglobin level, and to reveal the infl uence of metabolic hormones on erythrocytes microrheology in patients with DM-2. Materials and methods. In 30 patients with DM-2 we determined hemorheological parameters, blood viscosity, erythrocytes deformability (ED), erythrocytes aggregation (EA), glucose and glycated hemoglobin content, plasma proteins (albumins, globulins, fibrinogen) levels. In in vitro studies we recorded the microrheological characteristics of erythrocytes after their incubation with glucose and with some metabolic hormones (insulin, glucagon, adrenaline). Results. Microrheological parameters in patients with DM-2 diff ered signifi cantly from the parameters in healthy individuals. Glucose and glycated hemoglobin values more evidently correlated with hemorheological parameters in patients with DM-2 than in healthy individuals: the correlation coeffi cient between EA and glucose concentration in patients with DM-2 was 0.660, in healthy individuals — 0.480. Under insulin stimulus ED signifi cantly increased and AE, on contrary, decreased (p < 0.05). During erythrocytes incubation with glucagon EA similar reduced in patients with DM-2 and in healthy individuals. Adrenaline in three concentrations moderately increased ED (p < 0.05), and the observed EA increasing was greatest at low hormone concentration (0.01 μM). Conclusion. Analysis of hemorheological parameters in patients with DM-2 showed disorders of blood fl uidity and decreasing of blood transport capacity. It was also shown that hormones involved in metabolism regulation had a direct effect on erythrocytes microrheology.

scholarly journals Determination of autoantibodies in dogs with diabetes mellitus

2021 ◽  
pp. 2694-2698
Author(s):  
Franco González-Villar ◽  
Francisco Pérez-Bravo
Keyword(s):  
Diabetes Mellitus ◽  
Detection System ◽  
Tyrosine Phosphatase ◽  
Normal Weight ◽  
Enzyme Linked Immunosorbent Assay ◽  
Acid Decarboxylase ◽  
Insulin Deficiency ◽  
Insulin Dependent ◽  
Islet Antigen ◽  
Type 1 Dm

Background and Aim: The classification of diabetes mellitus (DM) in dogs has been controversial as currently canine insulin-dependent DM is classified together with absolute insulin deficiency, non-insulin-dependent DM, and relative insulin deficiency. Studies on human autoantibodies evaluated in canines with DM, such as anti-glutamic acid decarboxylase (GAD65), anti-islet antigen 2 (IA2), and anti-zinc transporter isoform 8 (ZnT8), have been inconclusive. Thus, this study was designed to establish the serological profile of anti-GAD65, anti-IA2, and anti-ZnT8 antibodies in a group of dogs with and without DM. Materials and Methods: Sixty-one dogs, including 31 patients with DM (with and without insulin treatment) and 30 patients without DM (normal weight and obese), were included for determining autoantibodies using a human enzyme-linked immunosorbent assay (ELISA) detection system for type 1 DM. Results: This study found the presence of anti-IA2 antibodies in 58% of the sample (18/31 patients with DM); however, the presence of anti-GAD65 was not detected, and anti-ZnT8 was found in 3 (9.6%) patients with DM. Conclusion: This study showed a higher positive frequency of anti-IA2 antibodies in a sample of canine with DM, indicating that alterations in the signaling vesicle tyrosine phosphatase 2 lead to lower insulin release and thus to an increase in patients' glycemia. These preliminary results should be taken with caution and corroborated by a canine-specific assay when an ELISA is available for such determination.

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