scholarly journals LC-MS/MS and Cytotoxic Activity Analysis of Extract and Fraction of Calophyllum soulattri Stembark

2021 ◽  
pp. 356-364
Author(s):  
Muhamad Salman Fareza ◽  
Nur Amalia Choironi ◽  
Sri Sutji Susilowati ◽  
Melina Puspita Rini ◽  
Vyola Festihawa ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Stem Bark ◽  
Positive Control ◽  
Bark Extract ◽  
Ic50 Values ◽  
Stem Bark Extract ◽  
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Calophyllum soulattri (Sulatri), a plant from Clusiaceae family, has been empirically used as traditional medicine. In the present study, C. soulattri stem bark extract and fractions were evaluated for their toxicity against MCF-7 breast cancer cell. The extract and fraction’s chemical content was analyzed using the combination of liquid chromatography with mass spectrometry (LC-MS/MS). The results showed that methanol extract and n-hexane fractions have strong cytotoxic activity with IC50 values 93.6 and 36 µg/mL, respectively. Meanwhile, as the positive control, ethyl acetate and cisplatin fractions have IC50 values 233 and 16.2 µg/mL, respectively. The LC-MS/MS analysis showed that the extract and fractions contained polyporusterone A, poricoic acid D, polyporusterone F, esulentagenin, and 1-acetyl-3-(methoxy-carbonyl)-β-carboline. Therefore, C. soulattri stem bark extract and fractions have potential activity as an anticancer agent that was able to inhibit MCF-7 breast cancer cell growth.  

Cytotoxic activity and anti-cancer potential of Ontario grown onion extracts against breast cancer cell lines

2018 ◽  
Vol 8 (3) ◽  
pp. 159 ◽  
Author(s):  
Meghan Fragis ◽  
Abdulmonem I. Murayyan ◽  
Suresh Neethirajan
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Cytotoxic Activities ◽  
Cancer Line ◽  
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Background: Breast cancer is the most commonly diagnosed cancer and the second leading cause of cancer deaths among Canadian women. Cancer management through changes in lifestyle, such as increased intake of foods rich in dietary flavonoids, have been shown to decrease the risk associated with breast, liver, colorectal, and upper-digestive cancers in epidemiologic studies. Onions are high in flavonoid content and one of the most common vegetables. Additionally, onions are used in most Canadian cuisines.Methods: We investigated the effect of five prominent Ontario grown onion (Stanley, Ruby Ring, LaSalle, Fortress, and Safrane) extracts on two subtypes of breast cancer cell lines: a triple negative breast cancer line MDA-MB-231 and an ER+ breast cancer line MCF-7.Results: These onion extracts elicited strong anti-proliferative, anti-migratory, and cytotoxic activities on both the cancer cell lines. Flavonoids present in these onion extracts induced apoptosis, cell cycle arrest in the G2/M phase, and a reduction in mitochondrial membrane potential at dose-dependent concentrations. Onion extracts were more effective against MDA-MB-231 compared to the MCF-7 cell line. Conclusion: In this study, we investigated the extracts synthesized from Ontario-grown onion varieties in inducing anti-migratory, cytostatic, and cytotoxic activities in two sub-types of human breast cancer cell lines. Anti-tumor activity of these extracts depends upon the varietal and can be formulated into nutraceuticals and functional foods for the wellbeing of cancer patients. Overall, the results suggest that onion extracts are a good source of flavonoids with anti-cancerous properties.Keywords: onion extracts; flavonoids; anti-proliferative; breast cancer; cytotoxic activity

scholarly journals Indonesian Micromelum minutum Leave Extracts and Their Cytotoxic Activities toward Breast Cancer Cell Lines

2021 ◽  
Vol 53 (1) ◽  
pp. 109-117
Author(s):  
Ratna Asmah Susidarti ◽  
Edy Meiyanto ◽  
Muthi' Ikawati ◽  
Normaidah Normaidah ◽  
Nurramadhani A. Sida
Keyword(s):  
Breast Cancer ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
4T1 Cells ◽  
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Isolation and identification of compounds and pharmacological activity of the Micromelum minutum grown in some countries has been done, but the Indonesian M. minutum has not been studied, either phytochemically or pharmacologically. This study aimed to determine the cytotoxic activity of Indonesian M. minutum leave extracts toward MCF-7 and 4T1 breast cancer cell lines. The leaves were obtained from M. minutum grow in Bantimurung National Park, Bulusaraung, South Sulawesi, and then were macerated gradually in hexane, ethyl acetate, and methanol. The cytotoxic activity of obtained extracts was determined by MTT assay. The extraction yielded hexane (HEM), ethyl acetate (EEM), and methanol (MEM) extracts of 2.65, 6.12, and 6.49%, respectively. HEM was the most potent extract with IC50 values of 148 and 87 µg/mL on MCF-7 and 4T1 cells, respectively, followed by EEM (185 and 170 µg/mL). MEM possessed a weak potency with an IC50 value of 384 µg/mL on MCF-7 cells and was not toxic toward 4T1 cells. Therefore, HEM is important to be further investigated for its active constituents.

scholarly journals EXTRACTION OF PHENOLIC COMPOUNDS AS ANTIOXIDANTS FROM SOME PLANTS AND THEIR CYTOTOXIC ACTIVITY AGAINST BREAST CANCER CELL LINE

2017 ◽  
Vol 10 (7) ◽  
pp. 349
Author(s):  
Rabab Omran ◽  
Zahraa Mal Taee ◽  
Mohammed Jal Jassani ◽  
Hayder O Hashim
Keyword(s):  
Breast Cancer ◽  
Antioxidant Activity ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Normal Cell ◽  
Cancer Cell Line ◽  
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Objective: The objective of the study was to study the antioxidants and cytotoxic activities of phenolic extracts of some plants against breast cancer and normal cell lines.Methods: Phenolics were extracted from different parts of some plants (15) such as seeds, fruits, leaves, and rhizomes using methanol: ethanol: HCl: Distilled water at a ratio 50:29:1:20 (v:v:v:v). The contents of phenolics and flavonoids were estimated using gallic acid and quercetin as standards, respectively. The antioxidant activity was determined by 2,2ˋ-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging assay. Phenolic flavonoids were partially purified by adsorption chromatography using a silica gel column from selected plants and assayed their cytotoxic activity against breast cancer cell line Michigan Cancer Foundation-7 (MCF-7) and a normal cell line of non-tumorigenic fetal hepatic cell line (WRL-68).Results: The Cinnamomum zeylanicum extract had the highest phenolic and flavonoid contents were followed by Lawsonia inermis, Citrullus colocynthis, Syzygium aromaticum, Peganum nigrum, and Phoenix dactylifera. The antioxidant activity of Curcuma longa, P. dactylifera, C. colocynthis, Solanum melongena, and C. zeylanicum extracts had the highest ability to scavenge the free radicals. The acetone fraction of P. dactylifera and C. colocynthis extracts had the minimum inhibition dose that kills 50% of cells inhibitory concentration 50 values 156.91 μg and 1055.06 μg against MCF-7 and 372.86 μg and 153.8 μg against WRL-68, respectively. While the S. melongena extract had less effect on both cell lines.Conclusions: Phenolics as antioxidant substances had moderate or variable effectiveness on normal and cancer cell lines, and the highest concentrations were cancerous poison impact may be on normal cells over than cancer cells.

scholarly journals Steroids from The Stem Bark of Dysoxylum nutans (Meliaceae) and Their Cytotoxic Effect Against MCF-7 Breast Cancer Cell Lines

2020 ◽  
Vol 6 (2) ◽  
pp. 133-139
Author(s):  
Tri Mayanti ◽  
Nur Insani Amir ◽  
Dewa Gede Katja ◽  
Sofa Fajriah ◽  
Ahmad Darmawan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cytotoxic Effect ◽  
Stem Bark ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Chemical Structures ◽  
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Three steroids, 3α-hydroxystigmast-5(6), 22-diene-7-one (1), stigmasterol (2) and 3-hydroxy-7β-methoxystigmast-5(6)-ene (3), were isolated from the stem bark of Dysoxylum nutans. The chemical structures were identified by spectroscopic data, which includes IR, 1D-NMR, 2D-NMR, and HR-TOFMS as well as by comparing previously reported spectral data. Compounds 1-3 were tested for cytotoxic effect against MCF-7 breast cancer cell lines and compound 1 showed the strongest cytotoxic activity with an IC50 value of 20.13 ± 0.06 μM.

scholarly journals Cytotoxic activity of Macrosolen parasiticus (L.) Danser on the growth of breast cancer cell line (MCF-7)

2015 ◽  
Vol 11 (42) ◽  
pp. 156 ◽  
Author(s):  
Richard Lobo ◽  
Nimmy Kumar ◽  
Rajalekshmi Maheshwari ◽  
CS Shreedhara ◽  
VijayKumar Sodde
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Mcf 7

In vitro Cytotoxic Activity of Vatica diospyroides Symington Type LS Root Extract on Breast Cancer Cell Lines MCF-7 and MDA-MB-468

2013 ◽  
Vol 13 (2) ◽  
pp. 130-135 ◽  
Author(s):  
Theera Srisawat ◽  
Parinuch Chumkaew ◽  
Warapond Maichum ◽  
Yaowapa Sukpondma ◽  
Potchanapond Graidist ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Root Extract ◽  
Mcf 7
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