Pinus densiflora stem bark extract induces breast cancer cell death via oxidative stress mediated lysosomal membrane permeabilization

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
EJ Park ◽  
T Venkatesan ◽  
YW Choi ◽  
YK Kim
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Cell Death ◽  
Breast Cancer Cell ◽  
Pinus Densiflora ◽  
Stem Bark ◽  
Bark Extract ◽  
Lysosomal Membrane Permeabilization ◽  
Cancer Cell Death ◽  
Stem Bark Extract

scholarly journals LC-MS/MS and Cytotoxic Activity Analysis of Extract and Fraction of Calophyllum soulattri Stembark

2021 ◽  
pp. 356-364
Author(s):  
Muhamad Salman Fareza ◽  
Nur Amalia Choironi ◽  
Sri Sutji Susilowati ◽  
Melina Puspita Rini ◽  
Vyola Festihawa ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Stem Bark ◽  
Positive Control ◽  
Bark Extract ◽  
Ic50 Values ◽  
Stem Bark Extract ◽  
Mcf 7

Calophyllum soulattri (Sulatri), a plant from Clusiaceae family, has been empirically used as traditional medicine. In the present study, C. soulattri stem bark extract and fractions were evaluated for their toxicity against MCF-7 breast cancer cell. The extract and fraction’s chemical content was analyzed using the combination of liquid chromatography with mass spectrometry (LC-MS/MS). The results showed that methanol extract and n-hexane fractions have strong cytotoxic activity with IC50 values 93.6 and 36 µg/mL, respectively. Meanwhile, as the positive control, ethyl acetate and cisplatin fractions have IC50 values 233 and 16.2 µg/mL, respectively. The LC-MS/MS analysis showed that the extract and fractions contained polyporusterone A, poricoic acid D, polyporusterone F, esulentagenin, and 1-acetyl-3-(methoxy-carbonyl)-β-carboline. Therefore, C. soulattri stem bark extract and fractions have potential activity as an anticancer agent that was able to inhibit MCF-7 breast cancer cell growth.  

scholarly journals Simvastatin induces breast cancer cell death through oxidative stress up-regulating miR-140-5p

Aging ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 3198-3219 ◽  
Author(s):  
Fuliang Bai ◽  
Ze Yu ◽  
Xin Gao ◽  
Jiawei Gong ◽  
Lizhi Fan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Cell Death ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Death

Neuroprotective Effects of the Anthocleista Schweinfurthii Gilg. (Loganiaceae) Stem Bark Extract in Postmenopause-Like Model of Ovariectomized Wistar Rats

2019 ◽  
Vol 16 (1) ◽  
Author(s):  
Ngoungoure Madeleine Chantal ◽  
Dzeufiet Djomeni Paul Désiré ◽  
Bilanda Danielle Caude ◽  
Mengue Ngandena Yolande Sandrine ◽  
Mbolang Nguegang Lohik ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Plant Extract ◽  
Total Protein ◽  
Wistar Rats ◽  
Stem Bark ◽  
Bark Extract ◽  
Estradiol Valerate ◽  
Neuroprotective Effects ◽  
Protein Levels ◽  
Stem Bark Extract

Abstract Background Estrogen deficiency in postmenopausal period causes severe neuroendocrine changes in brain which influences memory and other nervous functions. Anthocleista schweinfurthii is used traditionally to treat female infertility and menopause related symptoms. This study was performed to investigate the potential neuroprotective effects of aqueous extract of Anthocleista schweinfurthii on brain in a postmenopause-like model of ovariectomized Wistar rats. Methods Thirty animals were sham-operated or ovariectomized (Ovx) 84 days after surgery, six groups of five rats each were daily treated orally during 28 days with: distilled water for groups 1 (sham-operated) and 2 (Ovx), estradiol valerate (group 3) and the three doses of extracts {groups 4, 5 and 6 (Ovx)}. Biochemical and histological evaluations focused on brain. Results Compared to sham-operated control, ovariectomy decreased total protein levels in brain (p<0.01) which was increased by plant extract at the dose of 300 mg/kg (p<0.05), underlying its anabolic properties. Ovariectomy significantly decreased magnesium levels in brain (p<0.001). Anthocleista schweinfurthii increased significantly magnesium levels (p<0.01), showing its capacity to act on synaptic conduction. Ovariectomy induced oxidative stress by increasing malondialdehyde levels (p<0.05) and decreasing reduced glutathione levels (p<0.05) in brain. The plant extract exhibited antioxidative activity by reducing malondialdehyde levels and increasing glutathione levels in brain. Damage in brain structure which was caused by ovariectomy disappeared following the treatment. Conclusions Results suggest that Anthocleista schweinfurthii may have neuroprotective effects in Ovx Wistar rats by increasing total protein, magnesium levels and reducing oxidative stress in brain.

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