scholarly journals Production and Application of Streptococcus iniae DNA Vaccine to Increase Immunity ff Nile Tilapia (Oreochromis niloticus)

2016 ◽  
Vol 18 (2) ◽  
pp. 61
Author(s):  
Sutanti Sutanti ◽  
Novi Megawati ◽  
Sugiyo H. Pranoto ◽  
Ratu Siti Aliah
Keyword(s):  
Dna Vaccine ◽  
Nile Tilapia ◽  
Challenge Test ◽  
Virulence Gene ◽  
Vaccine Antigen ◽  
Streptococcus Iniae ◽  
Vaccine Production ◽  
Isolation Method ◽  
Time To Death ◽  
Plasmid Isolation

DNA vaccine Streptococcus iniae is a third generation of vaccines based on the gene encoding a vaccine antigen. Pgm is DNA-binding protein that activates expression of several important virulence gene, including those encoding polysaccharide capsule. The pgm gene controlled by MBA promoter has constructed successfully as a candidate of DNA vaccine to against S. iniae infection in Nile Tilapia. The goals of this study were to production and application of DNA Vaccine S. iniae to increase the nile tilapia immunity.  Vaccine production was using plasmid isolation method from Escherichia coli dH5α containing pMBA-pgm. Vaccine verification was was accomplished by PCR using pgm gene S. iniae Fstrep and Rstrep specific primers, which revealed the 1,713 bp PCR product. Application of DNA vaccine was using 25, 50, 75, and 100ng/ml dosage with intraperitonial injection method .The challenge test was using 108 cfu/ml density of S. iniae. Observation test parameter were survival rate, relative percent survival, mean time to death, pgm gene, and organ histology such as liver, eyes, brain, spleen and kidney. Vaccine production was succeed using plasmid isolation method and containing pgm gene revealed 1713bp. the application of DNA vaccine was optimum at 50ng/ml dosage with SR value 96.667%, RPS value 88,461% and MTD value 4,6 days. The eyes histology shows opacity and exopthalmia, the other organ such as liver, spleen and kidney was necrosis. The DNA vaccine was optimum at 50ng/ml dosage.  

Efficacy of α-enolase-based DNA vaccine against pathogenic Streptococcus iniae in Nile tilapia ( Oreochromis niloticus )

Aquaculture ◽  
2017 ◽  
Vol 468 ◽  
pp. 102-106 ◽  
Author(s):  
Pattanapon Kayansamruaj ◽  
Ha Thanh Dong ◽  
Nopadon Pirarat ◽  
Dachrit Nilubol ◽  
Channarong Rodkhum
Keyword(s):  
Dna Vaccine ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Streptococcus Iniae ◽  
Nile Tilapia Oreochromis Niloticus

Vibrio harveyi virulence gene expression in vitro and in vivo during infection in black tiger shrimp Penaeus monodon

2020 ◽  
Vol 139 ◽  
pp. 153-160
Author(s):  
S Peeralil ◽  
TC Joseph ◽  
V Murugadas ◽  
PG Akhilnath ◽  
VN Sreejith ◽  
...  
Keyword(s):  
Gene Expression ◽  
Virulence Genes ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Challenge Test ◽  
Virulence Gene ◽  
Economic Losses ◽  
Virulence Gene Expression

Luminescent Vibrio harveyi is common in sea and estuarine waters. It produces several virulence factors and negatively affects larval penaeid shrimp in hatcheries, resulting in severe economic losses to shrimp aquaculture. Although V. harveyi is an important pathogen of shrimp, its pathogenicity mechanisms have yet to be completely elucidated. In the present study, isolates of V. harveyi were isolated and characterized from diseased Penaeus monodon postlarvae from hatcheries in Kerala, India, from September to December 2016. All 23 tested isolates were positive for lipase, phospholipase, caseinase, gelatinase and chitinase activity, and 3 of the isolates (MFB32, MFB71 and MFB68) showed potential for significant biofilm formation. Based on the presence of virulence genes, the isolates of V. harveyi were grouped into 6 genotypes, predominated by vhpA+ flaB+ ser+ vhh1- luxR+ vopD- vcrD+ vscN-. One isolate from each genotype was randomly selected for in vivo virulence experiments, and the LD50 ranged from 1.7 ± 0.5 × 103 to 4.1 ± 0.1 × 105 CFU ml-1. The expression of genes during the infection in postlarvae was high in 2 of the isolates (MFB12 and MFB32), consistent with the result of the challenge test. However, in MFB19, even though all genes tested were present, their expression level was very low and likely contributed to its lack of virulence. Because of the significant variation in gene expression, the presence of virulence genes alone cannot be used as a marker for pathogenicity of V. harveyi.

scholarly journals Investigation of the Prevalence, Virulence Genes, and Antibiogram of Motile Aeromonads Isolated from Nile Tilapia Fish Farms in Egypt and Assessment of their Water Quality

Animals ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1432
Author(s):  
Fatma A. El-Gohary ◽  
Eman Zahran ◽  
Eman A. Abd El-Gawad ◽  
Adel H. El-Gohary ◽  
Fatma M. Abdelhamid ◽  
...  
Keyword(s):  
Water Quality ◽  
Water Samples ◽  
Nile Tilapia ◽  
Bacterial Infections ◽  
Virulence Genes ◽  
Virulence Gene ◽  
Fish Farms ◽  
Antibiotic Resistant ◽  
Bacterial Diseases ◽  
Mar Index

The aquaculture industry is a fast-growing sector in Egypt; however, the progress of this industry is impeded by many challenges such as poor water quality and associated bacterial infections. Among others, Motile Aeromonas Septicemia (MAS), caused by aeromonads, is among the most important bacterial diseases affecting aquaculture due to its zoonotic potential. In the present work, motile aeromonads were isolated from water samples (n= 8) and Nile tilapia (n= 240) in four fish farms (farms I, II, III, and IV) in Kafr El-Sheikh province during the period March to August 2017. This step was followed by investigation of the prevalence and phenotypic, molecular, and histopathological characterization of aeromonads. In addition, antimicrobial susceptibility and virulence gene detection were analyzed. Interestingly, physicochemical water analysis revealed different ranges in relation to the fish farms and seasons. More importantly, Aeromonas isolates were phenotypically identified in 33.3% and 12.5% from fish and water samples, respectively. The highest prevalence of motile aeromonads (46.7%) was recorded from farm IV, and only 12.5% of water samples were positive for them. Out of 80 isolates, 65 (81.25%) were molecularly identified at the genus level using gyrase B (gyrB). The prevalence of the virulence genes detected in the isolated motile aeromonads was aerolysin (aer), 52.2%; elastase (ahp), 26.25%; hemolysin (hyl), 35%; and lipase (lip), 3.75%. The antibiogram profile revealed that the highest resistance of aeromonads isolates (80%) was recorded to chloramphenicol, kanamycin, and azithromycin. Meanwhile, lower resistance levels of 40%, 30%, and 20% were found for streptomycin, cefotaxime, and amoxicillin, respectively. The multiple antibiotic resistance (MAR) index values ranged between 0.27 and 0.82 of motile aeromonads isolates. Furthermore, the histopathological examinations of naturally diseased tilapia revealed widespread hepatocellular necrosis with diffuse, numerous rod-shaped bacteria in liver with melanomacrophages and lymphocytic depletion with edema and hemosiderosis in the spleen. Our findings provide an updated epidemiological baseline for future reference and highlight the likely role of the adverse impact of water quality in the outbreaks of motile aeromonads with special reference to virulence genes and antibiotic resistant traits.

Tilapia piscidin 4 (TP4) enhances immune response, antioxidant activity, intestinal health and protection against Streptococcus iniae infection in Nile tilapia

Aquaculture ◽  
2019 ◽  
Vol 513 ◽  
pp. 734451 ◽  
Author(s):  
Eman Zahran ◽  
Engy Risha ◽  
Samia Elbahnaswy ◽  
Hebatallah Ahmed Mahgoub ◽  
Amany Abd El-Moaty
Keyword(s):  
Immune Response ◽  
Antioxidant Activity ◽  
Nile Tilapia ◽  
Streptococcus Iniae ◽  
Intestinal Health

Growth response and acquired resistance of Nile tilapia, Oreochromis niloticus (L.) that survived Streptococcus iniae infection

2006 ◽  
Vol 37 (12) ◽  
pp. 1238-1245 ◽  
Author(s):  
Craig A Shoemaker ◽  
Chhorn Lim ◽  
Mediha Yildirim-Aksoy ◽  
Thomas L Welker ◽  
Phillip H Klesius
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Growth Response ◽  
Acquired Resistance ◽  
Streptococcus Iniae ◽  
Nile Tilapia Oreochromis Niloticus
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