scholarly journals EVALUATION OF ANTICANCER ACTIVITY OF PARKINSONIA ACULEATA LEAVES EXTRACT ON EHRLICH’S ASCITES CARCINOMA-INDUCED MICE.

2018 ◽  
Vol 11 (1) ◽  
pp. 390
Author(s):  
Prameela Rani A ◽  
Agarjuna Babu E ◽  
Prem Madhur S ◽  
Ravi Chandra Sekhara Reddy D ◽  
Phani Kumar K
Keyword(s):  
Cell Count ◽  
Anticancer Activity ◽  
Tumor Volume ◽  
Hematological Parameters ◽  
Ethanolic Extract ◽  
Viable Cell ◽  
Ehrlich Ascites Carcinoma ◽  
Parkinsonia Aculeata ◽  
Tumor Weight ◽  
Ehrlich Ascites

 Objective: The objective of the study was to investigate the anticancer activity of the ethanolic extract of Parkinsonia aculeata (EEPA) leaves. Methods: Anticancer activity of P. aculeata (EEPA) of leaf extract was evaluated in Swiss albino mice against Ehrlich ascites carcinoma (EAC) cell line at the doses of 200 and 400 mg/kg body weight orally. The extracts were administered for 14 consecutive days. 24 h of the last dose and 18 h of fasting, the mice were sacrificed, and the anticancer effect of EEPA was assessed by evaluating tumor volume, viable and nonviable tumor cell count, tumor weight, hematological parameters, and biochemical parameters of EAC bearing mice.Results: P. aculeata extracts showed a significant decrease in (p<0.01) tumor volume, viable cell count, tumor weight, and elevated the life span of EAC bearing mice. Hematological profile such as red blood cell, hemoglobin count reverted to normal level in EEPA treated mice. The extracts significantly (p<0.05) decreased the levels of lipid peroxidation and significantly (p<0.05) increased the levels of reduced glutathione, superoxide dismutase and catalase.Conclusion: The results showed that the EEPA was effective in inhibiting the tumor growth in ascitic models and that is comparable to 5-fluorouracil. 

scholarly journals EVALUATION OF ANTICANCER ACTIVITY OF PARKINSONIA ACULEATA LEAVES EXTRACT ON EHRLICH’S ASCITES CARCINOMA-INDUCED MICE.

2018 ◽  
Vol 11 (1) ◽  
pp. 390
Author(s):  
Prameela Rani A ◽  
Agarjuna Babu E ◽  
Prem Madhur S ◽  
Ravi Chandra Sekhara Reddy D ◽  
Phani Kumar K
Keyword(s):  
Cell Count ◽  
Anticancer Activity ◽  
Tumor Volume ◽  
Hematological Parameters ◽  
Ethanolic Extract ◽  
Viable Cell ◽  
Ehrlich Ascites Carcinoma ◽  
Parkinsonia Aculeata ◽  
Tumor Weight ◽  
Ehrlich Ascites

 Objective: The objective of the study was to investigate the anticancer activity of the ethanolic extract of Parkinsonia aculeata (EEPA) leaves. Methods: Anticancer activity of P. aculeata (EEPA) of leaf extract was evaluated in Swiss albino mice against Ehrlich ascites carcinoma (EAC) cell line at the doses of 200 and 400 mg/kg body weight orally. The extracts were administered for 14 consecutive days. 24 h of the last dose and 18 h of fasting, the mice were sacrificed, and the anticancer effect of EEPA was assessed by evaluating tumor volume, viable and nonviable tumor cell count, tumor weight, hematological parameters, and biochemical parameters of EAC bearing mice.Results: P. aculeata extracts showed a significant decrease in (p<0.01) tumor volume, viable cell count, tumor weight, and elevated the life span of EAC bearing mice. Hematological profile such as red blood cell, hemoglobin count reverted to normal level in EEPA treated mice. The extracts significantly (p<0.05) decreased the levels of lipid peroxidation and significantly (p<0.05) increased the levels of reduced glutathione, superoxide dismutase and catalase.Conclusion: The results showed that the EEPA was effective in inhibiting the tumor growth in ascitic models and that is comparable to 5-fluorouracil. 

scholarly journals EVALUATION OF ANTICANCER ACTIVITY OF ETHANOLIC EXTRACT OF CYPERUS KYLLINGIA ENDL. IN EHRLICH ASCITES CARCINOMA-INDUCED SWISS ALBINO MICE

2018 ◽  
Vol 11 (10) ◽  
pp. 482
Author(s):  
Amites Gangopadhyay ◽  
Mainak Chakraborty ◽  
Pallab Kanti Haldar ◽  
Nitai Chand Chaulya ◽  
Amitava Ghosh
Keyword(s):  
Life Span ◽  
Cell Count ◽  
Tumor Volume ◽  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
Ehrlich Ascites Carcinoma ◽  
Active Principle ◽  
Dependent Manner ◽  
Tumor Weight ◽  
Ehrlich Ascites

Objective: The purpose of the study was to evaluate the antitumor and antioxidant status of ethanolic extract of Cyperus kyllingia Endl. on Ehrlich ascites carcinoma (EAC)-treated mice.Methods: The determination of in vivo antitumor activity was performed using EAC cells inoculated mice groups (n=12). The groups were treated for 9 consecutive days with ethanolic extract of C. kyllingia (EECK) at the doses of 20 and 40 mg/kg b.w., respectively. After 24 h of the last dose, half of the mice were sacrificed and the rest were kept alive for assessment of increase in life span. The antitumor potential of EECK was assessed by evaluating tumor volume, viable and non-viable tumor cell count, tumor weight, hematological parameters, and biochemical estimations. Furthermore, antioxidant parameters were assayed by estimating liver tissue enzymes.Results: EECK showed direct cytotoxicity on EAC cell line in a dose-dependent manner. EECK exhibited significant (p<0.05) decrease in the tumor volume, viable cell count, tumor weight, and elevated the life span of EAC tumor-bearing mice. The hematological profile, biochemical estimations, and tissue antioxidant assay were reverted to normal level in EECK-treated mice.Conclusion: Experimental results revealed that EECK possesses potent antitumor and antioxidant properties. Further, research is going on to find out the active principle(s) of EECK for better understanding of mechanism of its antitumor and antioxidant activity.

In vitro anticancer activity of Astaxanthin

2021 ◽  
Vol 5 (3) ◽  
pp. 033-037
Author(s):  
Uma Nath U ◽  
Ravi. R ◽  
Sundara Ganapathy ◽  
Lal Prasanth
Keyword(s):  
Anticancer Activity ◽  
Tumor Volume ◽  
Hematological Parameters ◽  
Ehrlich Ascites Carcinoma ◽  
High Dose ◽  
Ehrlich Ascites ◽  
Shrimp Shell ◽  
Shrimp Shell Waste ◽  
Wbc Count

This study was designed to determine the in vitro anticancer potential of the Astaxanthin isolated from shrimp shell waste (ETC) against Ehrlich Ascites Carcinoma (EAC) induced cancer in swiss albino mice. The anticancer activity was assessed using in vitro cytotoxicAity, mean survival time, tumor volume and hematological studies. The reliable criteria for evaluating the potential of any anticancer agent is the prolongation of lifespan of the animal and decrease in WBC count of blood. The high dose of ETC (200 mg/kg, orally) significantly reduced the tumor growth which was demonstrated by increased lifespan of the mice and restoration of hematological parameters. ETC was also found to be cytotoxic in the in vitro parameter which shows that ETC possesses significant anticancer potential.

scholarly journals EVALUATION OF ANTICANCER ACTIVITY OF CUCUMIS CALLOSUS AGAINST EHRLICH’S ASCITES CARCINOMA BEARING MICE

2018 ◽  
Vol 11 (10) ◽  
pp. 438
Author(s):  
Siva Prasad Panda ◽  
Rajsekhar Reddy A ◽  
Uttam Prasad Panigrahy
Keyword(s):  
Cell Count ◽  
Anticancer Activity ◽  
Tumor Volume ◽  
Viable Cell ◽  
Viable Cell Count ◽  
Standard Drug ◽  
Tumor Weight ◽  
Eac Cells

Objective: Our previous research isolated Cucurbitacin B (CuB) and ebenone leucopentaacetate (ELP) from methanolic fruit extract of Cucumis callosus (MFCC). The fruits of C. callosus (Rottl.) Cogn. (Family: Cucurbitaceae) plant have been traditionally used for antioxidant, anti-inflammatory, and antidiabetic actions. The objective of this research was to evaluate in vitro and in vivo anticancer effect of MFCC on Ehrlich Ascites Carcinoma (EAC) cell lines.Methods: In vitro anticancer assay of MFCC and standard drug, 5-fluorouracil (5-FU) was evaluated using Trypan blue and 3-(4, 5-dimethylthiazol-yl)-2, 5-diphenyl tetrazolium bromide methods. In vivo anticancer activity of MFCC and 5-FU was also performed after 24h of EAC cells (2×106cells/ mouse) inoculation based on toxicity study for 9 consecutive days. The activity of the extract was assessed by the study of tumor volume, tumor weight, viable and non-viable cell count, hematological parameters, and biochemical estimations.Results: The MFCC showed the direct antitumor effect on EAC cells in a dose-dependent manner with an IG50 value of 0.61 mg/ml. Furthermore, MFCC (350 mg/kg) exhibited significant (p<0.01) decrease in tumor volume, tumor weight, and viable cell count of EAC-treated mice. Hematological profile, biochemical estimation assay significantly (p<0.01) reverted to normal level in MFCC, and 5-FU treated mice.Conclusion: The anticancer activity of fruits of C callosus is may be either due to the presence of CuB or/and ELP as phytoconstituent and the activity is comparable to standard drug 5-FU.

scholarly journals ANTI PROLIFERATIVE ACTIVITY OF CALAMUS ROTANG AS A SPOTLIGHT ON EHRLICH’S ASCITES CARCINOMA TREATED PERITONEAL AS WELL AS SOLID TUMOR MODEL

2018 ◽  
Vol 10 (1) ◽  
pp. 85
Author(s):  
Subeer Roy ◽  
Diksha Kumari ◽  
Mainak Chakraborty ◽  
Pallab Kanti Haldar
Keyword(s):  
Life Span ◽  
Cell Count ◽  
Anticancer Activity ◽  
Solid Tumor ◽  
Tumor Volume ◽  
Hematological Parameters ◽  
In Vitro Cytotoxicity ◽  
Control Group

Objective: Methanol extract of Calamus rotang (MECR) root was appraised as a spotlight for the candidate of anticancer activity through the vehicle (Ehrlich Ascites Carcinoma) on Swiss albino mice.Methods: In vitro cytotoxicity assay has been accessed by trypan blue and MTT assay. In vivo anticancer activity was done using EAC cells (2 × 106) where in each groups mice were 6. After treatment with MECR at the lower dose of 200 and higher dose of 400 mg/kg respectively for 9 d, half of the mice of each group were sacrificed and the rest were kept to check prolongation of life span. The anticancer potential of MECR was evaluated by tumor volume, viable and nonviable tumor cell count, tumor weight, hematological parameters, biochemical estimations and Furthermore, tissue antioxidant parameters. Besides, solid tumor activity was also inspected.Results: In MECR treated groups (200 and 400 mg/kg) tumor volume, packed cell volume and viable cell count was significantly lessened as compared to that of the EAC control group. Life span, most reliable criteria for anticancer study, increased quite surprisingly by 50% and 100% in a dose dependant manner while compared to EAC control group. The hematological, biochemical and liver tissue antioxidant parameter are significantly (p<0.05) restored along with solid tumor case study (solid tumor volume) towards the normal level after treatment with MECR.Conclusion: From the above study it can be inferred that the MECR has impressive anticancer activity in dose dependent way.

scholarly journals In vivo anticancer activities of Ni (II)-Benzoin thiosemicarbazone complex [Ni(BTSC)2] against ehrlich ascites carcinoma cells

2018 ◽  
Vol 23 ◽  
pp. 77-88
Author(s):  
SMM Ali ◽  
HM Zakir ◽  
SMS Shahriar ◽  
MH Sarkar ◽  
AK Dey ◽  
...  
Keyword(s):  
Cell Growth ◽  
Growth Inhibition ◽  
Anticancer Drug ◽  
Hematological Parameters ◽  
Ehrlich Ascites Carcinoma ◽  
Cell Growth Inhibition ◽  
Anticancer Activities ◽  
Swiss Albino Mice ◽  
Tumor Weight ◽  
Ehrlich Ascites

Cancer is a class of diseases in which a group of cells display uncontrolled growth, invasion and sometimes metastasis. In order to find out a new anticancer drug, Ni(II) complex with benzoin thiosemicarbazon was synthesized and characterized. Anticancer activities of Ni(BTSC)2 2 2 has been studied against Ehrlich Ascites Carcinoma (EAC) cells in Swiss albino mice by monitoring tumor cell growth inhibition, tumor weight measurement, survival time of tumor bearing Swiss albino mice. Hematological parameters were also studied in both normal and EAC bearing treated mice. The results were compared with those obtained with a standard anticancer drug bleomycin and the compound was found to possess pronounced anticancer effect. Maximum cell growth inhibition was found to be 77.15% after treatment with Ni(BTSC)2 at the dose of 8 mg/kg (i.p). About 69.56% enhancement of life span was found at 8 mg/kg (i.p).With the same dose Ni(BTSC)2 reduced the tumor weight by 52.17% at day 20. The hematological parameters (WBC, RBC, hemoglobin content and differential counts) were found to be significantly changed as compared to those of the normal mice. These parameters restored more or less towards normal when treated with the test compound.J. bio-sci. 23: 77-88, 2015

scholarly journals Antineoplastic Activities of MT81 and Its Structural Analogue in Ehrlich Ascites Carcinoma-Bearing Swiss Albino Mice

2010 ◽  
Vol 3 (1) ◽  
pp. 61-70 ◽  
Author(s):  
Sujata Maiti Choudhury ◽  
Malaya Gupta ◽  
Upal Kanti Majumder
Keyword(s):  
Cell Count ◽  
Tumor Volume ◽  
Ehrlich Ascites Carcinoma ◽  
Viable Tumor Cell ◽  
Structural Analogue ◽  
Mean Survival Time ◽  
Ehrlich Ascites ◽  
Eac Cells

Many fungal toxins exhibit in vitro and in vivo antineoplastic effects on various cancer cell types. Luteoskyrin, a hydroxyanthraquinone has been proved to be a potent inhibitor against Ehrlich ascites tumor cells. The comparative antitumor activity and antioxidant status of MT81 and its structural analogue [Acetic acid-MT81 (Aa-MT81)] having polyhydroxyanthraquinone structure were assessed against Ehrlich ascites carcinoma (EAC ) tumor in mice. The in vitro cytotoxicity was measured by the viability of EAC cells after direct treatment of the said compounds. In in vivo study, MT81 and its structural analogue were administered (i.p.) at the two different doses (5, 7 mg MT81; 8.93, 11.48 mg Aa-MT81/kg body weight) for 7 days after 24 hrs. of tumor inoculation. The activities were assessed using mean survival time (MST), increased life span (ILS), tumor volume, viable tumor cell count, peritoneal cell count, protein percentage and hematological parameters. Antioxidant status was determined by malondialdehyde (MDA) and reduced glutathione (GSH ) content, and by the activity of superoxide dismutase (SOD) and catalase (CA T). MT81 and its structural analogues increased the mean survival time, normal peritoneal cell count. They decreased the tumor volume, viable tumor cell count, hemoglobin percentage and packed cell volume. Differential counts of WBC, total counts of RBC & WBC that altered by EAC inoculation, were restored in a dose-dependent manner. Increased MDA and decreased GSH content and reduced activity of SOD, and catalase in EAC bearing mice were returned towards normal after the treatment of MT81 and its structural analogue. Being less toxic than parent toxin MT81, the structural analogue showed more prominent antineoplastic activities against EAC cells compared to MT81. At the same time, both compounds exhibit to some extent antioxidant potential for the EAC-bearing mice.

scholarly journals Antitumor Activity of Diospyros peregrina on Ehrlich Ascites Carcinoma in Mice

2011 ◽  
Vol 3 (2) ◽  
pp. 413-419 ◽  
Author(s):  
A. B. Raju ◽  
Venu Gopal Y ◽  
Ravindranath A ◽  
Kalpana G ◽  
Prabhakar Reddy V
Keyword(s):  
Antitumor Activity ◽  
Life Span ◽  
Tumor Volume ◽  
Methanol Extract ◽  
Viable Cell ◽  
Ehrlich Ascites Carcinoma ◽  
Tumor Inoculation ◽  
Tumor Bearing ◽  
Hematological Profile ◽  
Ehrlich Ascites

The methanol extract of Diospyros peregrina (Ebenaceae) bark (MEDP) were evaluated for antitumor activity against Ehrlich ascites carcinoma (EAC)-bearing swiss albino mice. The extract was administered at the doses of 200 and 400 mg/kg body weight per day for 14 days after 24 h of tumor inoculation. After the last dose and 18 h fasting, the mice were sacrificed. The present study deals with the effect of MEDP on the growth of transplantable murine tumor, life span of EAC-bearing hosts and hematological profile  MEDP caused significant (P < 0.01) decrease in tumor volume, packed cell volume, and viable cell count; and it prolonged the life span of EAC-tumor bearing mice. Hematological profile converted to more or less normal levels in extract-treated mice. The results indicate that MEDP exhibited significant antitumor activity in EAC-bearing mice. Keywords: Diospyros peregrina; Ehrlich ascites carcinoma; Antitumor. © 2011 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. doi:10.3329/jsr.v3i2.6787                J. Sci. Res. 3 (2), 413-419 (2011)

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