scholarly journals ANTIOXIDANT ACTIVITY OF CONJUGATED LINOLEIC ACID

Author(s):  
Lalithadevi B ◽  
Muthiah Ns ◽  
Satya Narayana Murty K

Objective: The objective of the present study is to evaluate the antioxidant effect of conjugated linoleic acid (CLA). CLA is a collective term for a group of positional and geometric isomers of linoleic acid with conjugated double bond system. CLA is found in milk products and ruminant meat. CLA has been proved to be having many health benefits, one of them one being antioxidant effect.Methods: Various methods are used to find out the antioxidant effect of CLA. They are 1,1- diphenyl-2-picrylhydrazyl free radical scavenging assay, hydroxy radical scavenging assay, nitrous oxide radical scavenging assay, reducing power method, and phosphomolybdenum antioxidant assay. All these methods were done to test the antioxidant activity of CLA.Results: Standards error of mean calculated for all the tests. Statistical analysis done using one-way analysis of variance between the groups and SPSS software version 20 is used. p<0.05 is considered statistically significant. All the tests are statistically significant.Conclusion: As p values for all the tests came significant, and it was concluded that CLA has got antioxidant property. CLA could protect membranes composed of 1-palmitoyl 2-linoleoyl phosphatidylcholine from oxidative modification under conditions of metal ion-dependent or -independent oxidative stress. Finally, it was concluded that CLA has got potential health benefits such as weight reduction, antiaging, and antioxidative properties.

2012 ◽  
Vol 531 ◽  
pp. 524-527
Author(s):  
Bao Qing Wang

Antioxidant activities of acetone and methanol extracts from leaves of Acanthopanax senticosus, one of traditional Chinese medicine consumed in the eastnorth region of China, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, methanol extracts and derivative were superior to that of control with IC50 were 298.58, 360.16 and 142.21μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 305.37, 400.39 and 156.69μg/mL. It was concluded that Acanthopanax senticosus and its derivatives might be a potential natural source of antioxidants.


2014 ◽  
Vol 955-959 ◽  
pp. 387-389 ◽  
Author(s):  
Bao Qing Wang

Antioxidant activities of acetone and ethyl acetate extracts from Metaplexis japonica Makino, one of famous medicine plants in the eastnorth region of China, named luomo in Chinese, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, ethyl acetate extracts and derivative were IC50 were 313.21, 266.92 and 118.78μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 285.09, 351.57 and 123.89μg/mL. It was concluded that Metaplexis japonica Makino and its derivatives might be a potential natural source of antioxidants .


Author(s):  
Pranabendu Mitra ◽  
Venkatesh Meda ◽  
Rick Green

The main objective of this research was to compare the retention of antioxidant activity and total anthocyanin content of Saskatoon berries dried by freeze drying, microwave-vacuum drying, thin layer hot air drying and vacuum drying. Antioxidant activity of berry samples was determined by DPPH radical scavenging and ABTS radical scavenging, and the pH differential method was used to determine total anthocyanin content of the berry samples. The results showed that the freeze dried Saskatoon berries exhibited the highest retention of anthocyanin and antioxidant activity among the dried samples, followed by microwave-vacuum dried berries, thin layer hot air dried berries and vacuum dried berries. There were significant differences between the berry samples at P<0.05.  DPPH radical scavenging and ABTS radical scavenging were correlated linearly with an R2 value of 0.99 at P<0.05 showing their effectiveness for the determination of the antioxidant activity of the Saskatoon berries. However, the DPPH radical scavenging assay was more effective than the ABTS radical scavenging assay. The results also showed that antioxidant activity of the berries was highly correlated with the total anthocyanin content of the fruit. The reduction of anthocyanin in dried berry samples was linearly correlated with the reduction of DPPH radical scavenging with an R2 value of 0.97 at P<0.05 and, also, linearly correlated with the reduction of ABTS radical scavenging with an R2 value of 0.88 at P<0.05.


2013 ◽  
Vol 2 (2) ◽  
Author(s):  
Pranabendu Mitra ◽  
Venkatesh Meda ◽  
Rick Green

The main objective of this research was to compare the retention of antioxidant activity and total anthocyanin content of Saskatoon berries dried by freeze drying, microwave-vacuum drying, thin layer hot air drying and vacuum drying. Antioxidant activity of berry samples was determined by DPPH radical scavenging and ABTS radical scavenging, and the pH differential method was used to determine total anthocyanin content of the berry samples. The results showed that the freeze dried Saskatoon berries exhibited the highest retention of anthocyanin and antioxidant activity among the dried samples, followed by microwave-vacuum dried berries, thin layer hot air dried berries and vacuum dried berries. There were significant differences between the berry samples at P<0.05.  DPPH radical scavenging and ABTS radical scavenging were correlated linearly with an R2 value of 0.99 at P<0.05 showing their effectiveness for the determination of the antioxidant activity of the Saskatoon berries. However, the DPPH radical scavenging assay was more effective than the ABTS radical scavenging assay. The results also showed that antioxidant activity of the berries was highly correlated with the total anthocyanin content of the fruit. The reduction of anthocyanin in dried berry samples was linearly correlated with the reduction of DPPH radical scavenging with an R2 value of 0.97 at P<0.05 and, also, linearly correlated with the reduction of ABTS radical scavenging with an R2 value of 0.88 at P<0.05.


2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Ayman El-Anany ◽  
Sami Althwab ◽  
Rehab Ali ◽  
Rehab F.M. Ali ◽  
Hassan Mousa

Purpose The purpose of this study is to evaluate the effect of the addition of dried lemongrass leaves (DLGL) powder, at different levels, on phenolics content, antioxidant activities, consumer acceptance and the inhibition of lipid peroxidation of roasted coffee (RC). Design/methodology/approach DLGL powder was incorporated at the levels of 0%, 2.5%, 5.0%, 7.5% and 10% of RC weight. The total flavonoids (TF), total phenolics (TP) and antioxidant activity measured using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and reducing power assay of RC, DLGL and binary mixture of them determined. The oxidative indices of coffee oil samples during storage were investigated. In addition, the sensory characteristics of RC fortified with different levels of DLGL powder were evaluated. Findings The TP content of DLGL powder was 1,100.32 mg/100 g DWb, nearly 1.2 times higher than found in RC beans. The TF content of RC enriched with 2.5%, 5.0%, 7.5% and 10% DLGL were found to be around 1.05, 1.10, 1.15 and 1.20 times higher than that in the control coffee samples. RC supplemented with various levels of DLGL powder showed higher DPPH radical scavenging and reducing power activities. At the end of the storage period (six months), the acid, peroxide, P-Anisidine and total oxidation value values of RC supplemented with 10% DLGL powder were about 1.94, 2.52, 2.60 and 2.59 times as low as in the control sample without any addition of DLGL powder, respectively. RC containing 2.5% and 5.0% DLGL powder had significantly (p < 0.05) the highest sensory scores. Consequently, the addition of DLGL in coffee at up to a 5% ratio may have potential health benefits. Practical implications RC containing 2.5% and 5.0% DLGL powder had significantly (p = 0.05) the highest sensory scores. Originality/value Consequently, the addition of DLGL in coffee at up to a 5% ratio may have potential health benefits.


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