Potential antioxidant and lipid peroxidation inhibition of coffee mixed with lemongrass (Cymbopogon citrates) leaves

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Ayman El-Anany ◽  
Sami Althwab ◽  
Rehab Ali ◽  
Rehab F.M. Ali ◽  
Hassan Mousa

Purpose The purpose of this study is to evaluate the effect of the addition of dried lemongrass leaves (DLGL) powder, at different levels, on phenolics content, antioxidant activities, consumer acceptance and the inhibition of lipid peroxidation of roasted coffee (RC). Design/methodology/approach DLGL powder was incorporated at the levels of 0%, 2.5%, 5.0%, 7.5% and 10% of RC weight. The total flavonoids (TF), total phenolics (TP) and antioxidant activity measured using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and reducing power assay of RC, DLGL and binary mixture of them determined. The oxidative indices of coffee oil samples during storage were investigated. In addition, the sensory characteristics of RC fortified with different levels of DLGL powder were evaluated. Findings The TP content of DLGL powder was 1,100.32 mg/100 g DWb, nearly 1.2 times higher than found in RC beans. The TF content of RC enriched with 2.5%, 5.0%, 7.5% and 10% DLGL were found to be around 1.05, 1.10, 1.15 and 1.20 times higher than that in the control coffee samples. RC supplemented with various levels of DLGL powder showed higher DPPH radical scavenging and reducing power activities. At the end of the storage period (six months), the acid, peroxide, P-Anisidine and total oxidation value values of RC supplemented with 10% DLGL powder were about 1.94, 2.52, 2.60 and 2.59 times as low as in the control sample without any addition of DLGL powder, respectively. RC containing 2.5% and 5.0% DLGL powder had significantly (p < 0.05) the highest sensory scores. Consequently, the addition of DLGL in coffee at up to a 5% ratio may have potential health benefits. Practical implications RC containing 2.5% and 5.0% DLGL powder had significantly (p = 0.05) the highest sensory scores. Originality/value Consequently, the addition of DLGL in coffee at up to a 5% ratio may have potential health benefits.

2010 ◽  
Vol 156-157 ◽  
pp. 1372-1377 ◽  
Author(s):  
Cheng Chu Liu ◽  
Guo Ling Zhao ◽  
Ya Ning Li ◽  
Zhuo Ping Ding ◽  
Qi Gen Liu ◽  
...  

The polyphenol and flavonoid contents and antioxidation capabilities, including total reducing power, DPPH radical scavenging capability and lipid peroxidation inhibition, of ethanol extracts of water hyacinth (Eichhomia crassipes) were investigated and compared with those extracted from red tide seaweed (Enteromorpha prolifera) and from the most common seaweed (Porphyra haitanensis). Results showed that E. crassipes contained higher amounts of polyphenols [6.68 mg gallic acid equivalents (GAE)/g] in the leaf than in the stem (4.37 mg GAE/g) or in P. haitanensis (4.27 mg GAE/g). The E. crassipes also contained higher contents of flavonoids [1524 and 453 mg rutin equivalents (RE)/g in leaf and stem, respectively] than did E. prolifera (233 mg RE/g). Accordingly, both the leaf and stem of E. crassipes had relatively higher reducing power [118.35 and 47.21 100μg ascorbic acid equivalents (AscAE)/g, respectively] and DPPH radical scavenging capability (74.6% for leaf and 62.7% for stem) when compared with E. prolifera (reducing power of 16.5 and DPPH radical scavenging capability of 42.96%). Statistical analysis showed that the antioxidation capabilities, especially reducing power, of extracts of the three aquatic plants were positively correlated with their total polyphenol contents, but not with the contents of flavonoids. The correlation coefficient (r) of the content of polyphenols and reducing power of those extracts was r=0.9028, that for DPPH scavenging and lipid peroxidation inhibition was r=0.9311 and 0.9099, respectively. These results indicated that phenolic compounds are the main compounds contributing to the antioxidation activities of seaweed extracts. E. crassipes can be a source for extracting natural antioxidants.


2012 ◽  
Vol 550-553 ◽  
pp. 1784-1789 ◽  
Author(s):  
Ting Qiu ◽  
Bi Yu Wang ◽  
Xiao Cui Lin ◽  
Yan Xiang Wu

This study aimed at evaluating the antioxidative activity of crude hsian-tsao extracted by acetone and the Fraction E purified by AB-8 macroporous resin chromatography column. The antioxidative activities, including the DPPH radical-scavenging effects, Fe2+-chelating ability, and reducing power as well as the inhibition of lipid peroxidation, were studied. Results demonstrated that both crude extract and Fraction E were more effective in the inhibition of lipid peroxidation and the DPPH radical-scavenging ability than that in the Fe2+-chelating ability. Based on the inhibition of lipid peroxidation, the order of the antioxidant activities was follows: Fraction E >GA> crude extract >Vc. Fraction E seemed to be a promising product of natural antioxidants enriched with phenolic compounds


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Song Yu ◽  
Xuetian Zhu ◽  
Helin Yang ◽  
Lihe Yu ◽  
Yifei Zhang

AbstractSeed deterioration, coupled with a decrease in nutrients, is unavoidable following long-term storage, and these seeds are therefore used as livestock fodder. Here, we developed a simple, rapid and efficient method of producing high amounts of antioxidants from deteriorated seeds via melatonin-induced germination. Legume seeds were subjected to high humidity at 55 °C for 12–36 h to obtain aged seeds with a 40% germination rate and severely reduced antioxidant nutrition (total phenolics content, ferric reducing power and 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging capacity). Aged seeds were then treated with 0.1 mM melatonin, resulting in the production of sprouts with a higher total phenolics content (fivefold), greater ferric reducing power (sevenfold) and greater DPPH radical scavenging capacity (twofold) compared to the aged seeds. These findings suggest that melatonin treatment efficiently converted aged seed reserve residues into antioxidant nutrients, providing an alternative use for deteriorated seeds in food production.


2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Nur Sumirah Mohd Dom ◽  
Nurshieren Yahaya ◽  
Zainah Adam ◽  
Nik Mohd Afizan Nik Abd. Rahman ◽  
Muhajir Hamid

The present study aimed to evaluate the potential of standardized methanolic extracts from seven Ficus deltoidea varieties in inhibiting the formation of AGEs, protein oxidation, and their antioxidant effects. The antiglycation activity was analyzed based on the inhibition of AGEs, fructosamine, and thiol groups level followed by the inhibition of protein carbonyl formation. The antioxidant activity (DPPH radical scavenging activity and reducing power assay) and total phenolic contents were evaluated. After 28 days of induction, all varieties of Ficus deltoidea extracts significantly restrained the formation of fluorescence AGEs by 4.55–5.14 fold. The extracts also reduced the fructosamine levels by 47.0–86.5%, increased the thiol group levels by 64.3–83.7%, and inhibited the formation of protein carbonyl by 1.36–1.76 fold. DPPH radical scavenging activity showed an IC50 value of 66.81–288.04 μg/ml and reducing power activity depicted at 0.02–0.24 μg/ml. The extent of phenolic compounds present in the extracts ranged from 70.90 to 299.78 mg·GAE/g. Apart from that, correlation studies between the activities were observed. This study revealed that seven varieties of Ficus deltoidea have the potential to inhibit AGEs formation and possess antioxidant activity that might be attributed to the presence of phenolic compounds.


Author(s):  
Jane-Rose I. Oche ◽  
Titilayo O. Johnson ◽  
Augustina O. Akinsanmi ◽  
Kiri H. Jaryum ◽  
Timothy Francis

Aim: The aim of this study was to investigate and compare the antioxidative properties of the mistletoe plant obtained from three different host species namely Psidium guajava, Vernonia amygdalina and Moringa olifera lam. Study Design: Experimental Design Place and Duration of Study: Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences and Department of Biochemistry, College of Health Sciences, University of Jos, Nigeria. Methodology: Crude methanolic leaf extracts were studied for their antioxidative properties; Iron reducing and Iron-chelating activities, Nitric oxide (NO) radical and 2,2-diphenyl 1-picrylhydrazyl (DPPH) radical scavenging activities and the lipid peroxidation and thiobarbituric acid reaction (TBAR) methods. One way ANOVA was used for the result analysis with P<.05 for significant difference. Results: Mistletoes from Psidum guajava (MSPG) had significantly higher reducing property (0.16 – 0.20mg/mL); the chelating property of Mistletoes from Moringa olifera (MSMO) was significantly lower (45.7 – 58.9%); DPPH radical scavenging activity had no significant difference; and Nitric oxide scavenging activity was significantly higher in MSPG (72.1% in 75mg/mL) than the extracts from other hosts. MSPG had significantly higher TBAR inhibition using both FeSO4 (77.8% at 125µg/mL) and Sodium nitroprusside (61.6+1.0% at 125µg/mL) with an IC50 of 30.27µg/mL . Extract of Tapinanthus globiferus leaves from Psidium guajava had more antioxidative activities in the TBARs followed by Tapinanthus globiferus leaf extract from Vernonia amygdalina (MSVA). Conclusion: From the study, mistletoes from Psidium guajava had higher antioxidant activity compared to other hosts, which probably justifies its use for treatment of cancer in traditional medicinal practice.


2019 ◽  
Vol 121 (12) ◽  
pp. 3208-3232 ◽  
Author(s):  
Hamed Hosseini ◽  
Shadi Bolourian ◽  
Fakhri Shahidi

Purpose Jujube fruit (JF) (Ziziphus jujuba Mill.) is used as pharmaceuticals food, flavors and food additives. The purpose of this paper is to study the suitability of JF incorporation into a commercial sponge cake formulation, and to produce a nutritious bakery product with appropriate organoleptic and technological characteristics. Design/methodology/approach The optimal level of JF was incorporated into sponge cake using a custom mixture design with three independent variables, namely, refined wheat flour (RWF, 15–28 percent), sugar (7–22 percent) and JF (0–28 percent), as well as several responses, including physical properties, texture profile analysis (TPA), sensorial evaluation and color features. Moreover, physicochemical properties (TPA and oxidative indices) of optimal cake (two of the best JF levels) were compared with control sample (without JF) during 51 days storage period, while two baking temperatures (180°C and 170°C) were used. Findings The optimal amounts of RWF (21.19 percent), sugar (21.20 percent) and JF (7.61 percent) required for making the sample with maximum springiness, cohesiveness, specific volume, sensorial scores and yellowness, as well as the lowest firmness, baking loss and browning were determined. Desirable effects of JF on the cake quality well maintained throughout the storage period, as TPA attributes, peroxide value, ultraviolet absorbance and acid value showed less changes in JF-incorporated cake than the control sample. Originality/value Incorporating JF (~7 percent) into the batter was successful to improve the physicochemical properties in both fresh and stored cake with chocolate-like color.


2018 ◽  
Vol 13 (11) ◽  
pp. 1934578X1801301 ◽  
Author(s):  
Ying Zou ◽  
Min Zhang ◽  
Tingrui Zhang ◽  
Junwen Wu ◽  
Jun Wang ◽  
...  

The flavonoid fraction was obtained from Elsholtiza bodinieri Vaniot (EBV) by ethanol-reflux and liquid-liquid extraction. The total content of flavonoid was 179.55 mg/g, and the purity was 64.6%. Then cynaroside with the purity of 94% was isolated from the fraction by preparative HPLC and characterized by the combined usage of HPLC, ESI-MS, and NMR. The antioxidant activity of cynaroside was determined using 2 complementary methods, namely, 2,2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and reducing power assay. The anti-inflammatory effect of cynaroside was investigated based on in-vitro and in-vivo experiment. The results showed that cynaroside from EBV scavenged DPPH radical and reduced Fe3+ to Fe2+ effectively, inhibited NO and ROS production in LPS-stimulated RAW264.7 cells and attenuated the inflammation in the mouse model significantly ( p < 0.01), which showed it to be a nutraceutical product in the food industry.


2019 ◽  
Vol 78 ◽  
pp. 02008
Author(s):  
Hong-li Zhou ◽  
Bing Li ◽  
Mei-fu Wu ◽  
Ye Liu

To determine the effect of different harvesting time on antioxidant capacity in Jerusalem artichoke polysaccharides (inulin), the Jerusalem artichoke before and after overwintering were collected from the same region, and then evaluate their antioxidant capacity in vitro by reducing power assay, DPPH radical-scavenging assay and hydroxyl radical-scavenging assay. Ascorbic acid at similar mass concentration was served as positive control. The results showed no significant difference were observed in reducing power when the absorbance values were about 0.3. When the mass concentrations before and after overwintering were 0.9 mg/mL and 1.7 mg/mL, the DPPH radical-scavenging rate both reached at 50%. And when the mass concentrations were both 10 mg/mL, the hydroxyl radical-scavengings were 35.8% and 27.9%, respectively. Thus, it could be concluded that the antioxidant capacity in Jerusalem artichoke polysaccharides before overwintering was higher than that after overwintering, and showed a good dose-dependent manner with its mass concentration.


2010 ◽  
Vol 5 (6) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Kuo-Chen Cheng ◽  
Man-Chun Hsueh ◽  
Hou-Chien Chang ◽  
Alan Yueh-Luen Lee ◽  
Hui-Min Wang ◽  
...  

Two novel antioxidants, obtusilactone A (1) and (-)-sesamin (2) have been identified in Cinnamomum kotoense Kanehira. Both showed effective 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity compared with vitamin C, and reducing power compared with BHA. These results suggest that these constituents of C. kotoense act as natural antioxidants and play a potential role in cancer prevention.


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