scholarly journals ULTRAVIOLET SPECTROPHOTOMETRIC METHOD DEVELOPMENT FOR ESTIMATION OF NEW ANTIVIRAL REPURPOSING DRUG FAVIPIRAVIR

2021 ◽  
pp. 67-69
Author(s):  
JEEVANA JYOTHI B ◽  
VENKATA KAVYA R
Keyword(s):  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Spectrophotometric Method ◽  
Method Development ◽  
Quantitation Limit ◽  
Ich Guidelines ◽  
Relative Standard ◽  
Recovery Study ◽  
Development And Validation ◽  
Interday Precision

Objective: A new, economical, precise, linear, sensitive, accurate, ultraviolet (UV) spectrophotometric method for the estimation of new antiviral repurposing drug favipiravir as there is no reported simple UV spectrophotometric method for estimation. The efforts were made for development and validation of favipiravir as per ICH guidelines, because drug has a wide scope for formulations to be developed for treating different viruses. Methods: This method was developed using ethanol and water as a solvent. Favipiravir showed the absorption maxima at 234 nm. A Shimadzu UV–visible spectrophotometer (UV JAPAN 1801) was used to carry out spectral analysis. Results: The developed method was linear for a range of 0–10 μg/ml and displayed a good correlation coefficient of 0.9995. Accuracy of the method was estimated using a recovery study. The amount of drug recovered was found to be in the range of 99.30–99.91%. The % relative standard deviation value of intraday precision was found to be 0.408% and interday precision was found to be in the range of 0.348–0.693%. The % relative standard deviation found to be <2 which are indicative of the precision and reproducibility of the method. Detection limit and quantitation limit were noticed as 0.095 and 0.290, respectively. Conclusion: The developed UV spectrophotometric method was validated statistically for linearity, accuracy, precision, and sensitivity and results proved that the method can be employed for routine analysis of favipiravir.

scholarly journals Development and validation of spectrophotometric method for phenylephrine hydrochloride estimation in nasal drops formulations

2008 ◽  
Vol 27 (2) ◽  
pp. 149 ◽  
Author(s):  
Ivana Savić ◽  
Goran Nikolić ◽  
Vladimir Banković
Keyword(s):  
Standard Deviation ◽  
Sodium Hydroxide ◽  
Relative Standard Deviation ◽  
Spectrophotometric Method ◽  
Molar Absorptivity ◽  
Dosage Forms ◽  
Phenylephrine Hydrochloride ◽  
Relative Standard ◽  
Development And Validation

Simple, accurate and reproducible UV-spectrophotometric method was developed and validated for the estimation of phenylephrine hydrochloride in pharmaceutical nasal drops formulations. Phenylephrine hydrochloride was estimated at 291 nm in 1 mol⋅dm-3 sodium hydroxide (pH 13.5). Beer’s law was obeyed in the concentration range of 10–100 μg⋅cm−3 (r2 = 0.9990) in the sodium hydroxide medium. The apparent molar absorptivity was found to be 1.63×103 dm3⋅mol−1⋅cm−1. The method was tested and validated for various parameters according to the ICH (International Conference on Harmonization) guidelines. The detection and quantitation limits were found to be 0.892 and 2.969 μg⋅cm−3, respectively. The proposed method was successfully applied for the determination of phenylephrine hydrochloride in pharmaceutical nasal drops formulations. The results demonstrated that the procedure is accurate, precise and reproducible (relative standard deviation < 1 %), while being simple, cheap and less time consuming, and hence can be suitably applied for the estimation of phenylephrine hydrochloride in different dosage forms.

scholarly journals UV Spectrophotometric Method Development and Validation of Fimasartan Drug and Its Tablet Formulation

1970 ◽  
Vol 7 (5) ◽  
pp. 25-29
Author(s):  
Kaushik S Agrawal ◽  
Lokesh R Gandhi ◽  
Nitin S. Bhajipale S Bhajipale3
Keyword(s):  
Standard Deviation ◽  
Quantitative Determination ◽  
Relative Standard Deviation ◽  
Spectrophotometric Method ◽  
Method Development ◽  
Cost Effective ◽  
Tablet Formulation ◽  
Relative Standard ◽  
Bulk Drug

A novel, safe and sensitive method of Spectrophotometric estimation in UV - region has been developed for the assay of Fimasartan in its tablet formulation. The present study was undertaken to develop and validate a simple, accurate, precise, reproducible and cost effective UV spectrophotometric method for the estimation of Fimasartan bulk and pharmaceutical formulation. The method have been developed and validated for the assay of Fimasartan using Methanol as diluent. Absorption maximum (λmax) of the drug was found to be 240nm. The quantitative determination of the drug was carried out at 240nm. The method was shown linear in the mentioned concentrations having correlation coefficient R2 of 0.999. The recovery values for Fimasartan ranged from 98.74% to 99.23%.The Percent Relative Standard Deviation of interday and intraday was 0.85% and 0.75% respectively. All the parameters of the analysis were chosen according to the International Conference on Harmonisation guideline and validated statistically using Relative Standard Deviation and Percent Relative Standard Deviation. Hence, proposed method was precise, accurate and cost effective. This method could be applicable for quantitative determination of the bulk drug as well as dosage formulation.   KEY WORDS: 

UV-SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF SAXAGLIPTIN IN API AND PHARMACEUTICAL DOSAGE FORM

2020 ◽  
pp. 63-66
Author(s):  
BHAGYASHRI S. SHINDE ◽  
M. S. KALSHETTI ◽  
ANJALI P. KOKANE
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Method Development ◽  
Pharmaceutical Formulation ◽  
Pharmaceutical Dosage Form ◽  
Ich Guidelines ◽  
Recovery Study ◽  
Method Development And Validation ◽  
Curve Method ◽  
Development And Validation

Objective: A new sensitive, economical, simple, rapid UV spectrophotometric method has been developed for the estimation of Saxagliptin in API and pharmaceutical formulation. Methods: Calibration curve method was used for the estimation of Saxagliptin in API and pharmaceutical formulation. Results: The method was validated for linearity, range, accuracy, precision, robustness, LOD, LOQ. Linearity was found in the range of 10-60µg/ml. Accuracy was performed by using a recovery study. The amount of drug recovered was found to be in the range of 99.01-100.1%. All the parameters were validated as per the ICH guidelines. Conclusion: This method is suitable forused routine analysis of present Saxagliptin in API and Pharmaceutical dosage form.

scholarly journals IMPORTANCE OF THE OXIDATION REACTION OF SODIUM METAPERIODATE FOR SPECTROPHOTOMETRIC ASSAY OF RESERPINE

2004 ◽  
Vol 72 (2) ◽  
pp. 121-129
Author(s):  
D. K. Singh ◽  
Archana Sahu
Keyword(s):  
Acetic Acid ◽  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Spectrophotometric Method ◽  
Oxidation Reaction ◽  
Spectrophotometric Assay ◽  
Sodium Metaperiodate ◽  
Relative Standard ◽  
Sensitive Spectrophotometric Method

A new simple, accurate and reasonably sensitive spectrophotometric method is described for the determination of reserpine based on the oxidation with sodium metaperiodate in the presence of acetic acid and coupling with 3-methyl-2-benzo thiazolinone hydazone hydrochloride (MBTH) to form highly blue coloured product showing maximum absorbance at 630 nm. Beer's law is obeyed in the range 0.2–1.6 µg ml−1. The relative standard deviation was found to be less than 1%. The proposed method can be applied for the estimation of reserpine in tablets with no evidence of interference from excipients.

scholarly journals Spectrophotometric Method for Determination of Paraquat in Food and Biological Samples

1997 ◽  
Vol 80 (2) ◽  
pp. 388-391 ◽  
Author(s):  
Ritu Kesari ◽  
Manish Rai ◽  
Vinay Kumar Gupta
Keyword(s):  
Standard Deviation ◽  
Metal Ions ◽  
Relative Standard Deviation ◽  
Alkaline Medium ◽  
Spectrophotometric Method ◽  
Biological Samples ◽  
Molar Absorptivity ◽  
Relative Standard ◽  
Sensitive Spectrophotometric Method

Abstract A sensitive spectrophotometric method was developed for determination of paraquat, a widely used herbicide. Paraquat was reduced with glucose in an alkaline medium, and the blue radical ion obtained was measured at 600 nm. Beer’s law was obeyed at 0.1–1.2 ppm paraquat. The molar absorptivity was 1.26 × 105 L mol-1 cm-1. The standard deviation and relative standard deviation were ± 0.007 and 2.0%, respectively, for 5 μg paraquat/10 mL analyzed over 7 days. The method was free from interference by other commonly used pesticides and metal ions. The method may be used to the determine paraquat in plants, fruits, grains, water, blood, and urine.

scholarly journals Rapid and Selective UV Spectrophotometric Method for the Analysis of Ceftazidime

2009 ◽  
Vol 92 (3) ◽  
pp. 820-823 ◽  
Author(s):  
Andréia de Haro Moreno ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Quantitative Evaluation ◽  
Spectrophotometric Method ◽  
Pharmaceutical Preparations ◽  
Pure Form ◽  
Uv Detector ◽  
Relative Standard ◽  
Beer's Law ◽  
Beer’S Law

Abstract A new UV spectrophotometric method was developed for quantitative evaluation of ceftazidime preparations. The UV detector was set at 255 nm. Beer's law is obeyed in the concentration range of 7.014.0 g/mL. The method was found to be selective, linear, accurate, and precise in the specified ranges. Intra- and interday variability for the method were &lt;2 relative standard deviation. Common excipients used as additives in pharmaceutical preparations do not interfere with the proposed method. This method was successfully used for quantification of ceftazidime in pure form and in pharmaceutical preparations.

scholarly journals Application of Derivative Spectrophotometry for Simultaneous Determination of Quinapril and Hydrochlorothiazide in the Combination Tablets

2004 ◽  
Vol 87 (4) ◽  
pp. 847-851 ◽  
Author(s):  
Dorota Kowalczuk ◽  
Hanna Hopkała
Keyword(s):  
Standard Deviation ◽  
Simultaneous Determination ◽  
Relative Standard Deviation ◽  
Spectrophotometric Method ◽  
Derivative Spectrophotometry ◽  
Order Derivative ◽  
Zero Crossing ◽  
Relative Standard ◽  
Separation Of Components

Abstract A new second-order-derivative spectrophotometric method using zero-crossing technique measures quinapril (QUI) and hydrochlorothiazide (HYD) in 2-component mixtures. The procedure does not require prior separation of components from the sample. QUI was determined at a wavelength of 211.6 nm (zero-crossing wavelength point of HYD). Similarly, HYD was measured at 270.8 nm (zero-crossing wavelength point of QUI). Calibration graphs were constructed over the concentration range of 4.0 to 24.0 μ/mL for QUI and 2.5 to 15.0 μg/mL for HYD. Detection and quantitation limits were 0.85 and 2.5 μg/mL for QUI and 0.12 and 0.4 μg/mL for HYD, respectively. The accuracy (recovery 100.5–102%), precision (relative standard deviation less than 3.5% for QUI and 1.5% for HYD), selectivity, and sensitivity of the elaborated methods were satisfactory. The proposed method was applied successfully for the determination of both drugs in QUI-HYD tablets.

Kinetic Spectrophotometric Method for the Determination of Selenium (IV) by its Catalytic Effect on the Reduction of Arsenazo III with Sodium Hypophosphite in Micellar Media

2012 ◽  
Vol 554-556 ◽  
pp. 926-933
Author(s):  
Zhi Rong Zhou ◽  
Li Zhen Zhang
Keyword(s):  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Spectrophotometric Method ◽  
Catalytic Effect ◽  
Fixed Time ◽  
Sodium Hypophosphite ◽  
Micellar Media ◽  
Relative Standard ◽  
Kinetic Spectrophotometric

A simple and sensitive kinetic spectrophotometric method for the determination of trace selenium (IV) is described, based on its catalytic effect on the reduction arsenazo III (AsA III) with sodium hypophosphite (NaH2PO2) in a solution of 0.02 mol/L sulfuric acid and in the presence of cationic micellar media. The reaction rate is monitored spectrophotometrically by measuring the decrease in absorbance of AsA III at 550 nm with a fixed-time method. The decrease in the absorbance of AsA III is proportional to the concentration of Se(IV) in the range 0.16–1.0 µg/L after a fixed time of 4–10 min from the initiation of the reaction. The limit of detection is 0.049 µg/L Se(IV). The influence of th e factors such as acidity, concentration of reactants, type and concentration of surfactants, reactive time, temperature and co-existing ions on the reaction is discussed. The optimum reaction conditions of reaction are established and some kinetic parameters are determined; the apparent activation energy of catalytic reaction is 59.51 kJ/mol. The relative standard deviation for eleven replicate determination of 0.02, and 0.01µg/25mL Se(IV) was 2.0 % and 2.4 %, respectively. Combined with sulphydryl dextrane gel (SDG) separation and enriching, the method has been successfully applied to the determination of Se (IV) in foodstuff and human hair samples with the relative standard deviation of 2.1 %–5.8 % and the recovery of 97.0 %–103.3 %, the results are in good agreement with those provided by ICP-AES method.

The Determination of Nitrate in Tobacco by UV-Spectrometry / Die Bestimmung von Nitrat in Tabak durch UV-Spektrometrie

1966 ◽  
Vol 3 (7) ◽  
Author(s):  
H. Barkemeyer
Keyword(s):  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Spectrophotometric Method ◽  
Uv Spectrometry ◽  
Relative Standard

AbstractA new spectrophotometric method, suitable for routine determination of nitrate in tobacco, is described. Quantities as low as 0.05 % of nitrate in tobacco are determinable, the results can be reproduced very well (RSD (relative standard deviation) = 1.43 % for 0.7 % of nitrate in tobacco). 15-20 determinations per day and per person can be performed. The figures obtained by this method are identical with those obtained by other methods. Interferences by other compounds have not been observed. Commercial cigarettes show contents of nitrate in tobacco in the range between 2 % and 0.04 %.

Spectrophotometric Determination of Boron in Dates of Some Cultivars Grown in Saudi Arabia

1993 ◽  
Vol 76 (3) ◽  
pp. 601-603 ◽  
Author(s):  
A A Al-Warthan ◽  
S S Al-Showiman ◽  
S A Al-Tamrah ◽  
A A BaOsman
Keyword(s):  
Saudi Arabia ◽  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Molar Absorptivity ◽  
Relative Standard ◽  
Sensitive Spectrophotometric Method

Abstract The formation of a red complex between boron and the quinalizarin reagent was investigated and used as the basis for a simple and sensitive spectrophotometric method for boron in date cultivars. At 620 nm, the absorbance was linear (r= 0.999) over the 0.25-2.5 μg/mL concentration range. The molar absorptivity was found to be 2.23 x 103mol-1cm-1 and the relative standard deviation for 10 replicates (1.0 μg/mL) was 0.97%.

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