SALIVARY ALPHA AMYLASE LEVELS DURING PRIMARY TOOTH EXTRACTION IN CHILDREN AGED 6–11 YEARS

Objective: Patients generally experience some level of anxiety during dental treatments. Salivary alpha amylase (SAA) can be used as a biomarker for anxiety. This study aimed to compare SAA levels between children who had never and had undergone tooth extraction procedures with a local anesthetic injection.Methods: Thirty-seven participants aged 6–11 years who were to undergo the extraction of primary teeth at the Dental and Oral Educational Hospital, Faculty of Dentistry Universitas Indonesia, were enrolled. The subjects were divided into two groups: One group consists of 20 children who had never undergone a tooth extraction, and the other group consists of 17 children who had undergone tooth extraction. From all children, one saliva sample was collected using a portable saliva strip 10 min before (t0), shortly after (t1), and 10 min after (t2) local anesthetic injection, and the SAA activity was determined using a portable Nipro Cocoro Meter device. The SAA levels of both groups were analyzed using the Mann-Whitney U test.Results: There were significant differences in SAA levels at t0 (p=0.001), t1 (p=0.018), and t2 (p=0.021) in both groups. Anxiety is a combination of behavioral and physiological reactions. SAA release is regulated by autonomic innervation, and the SAA level increases owing to acute stress.Conclusion: Dentists should note patients’ negative dental experiences to provide more effective and less traumatic treatment.

Download Full-text

CORRELATION BETWEEN WONG-BAKER FACES PAIN SCALE AND SALIVARY ALPHA-AMYLASE LEVEL IN CHILDREN AGED 6–11 YEARS

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2017.v9s2.34 ◽

2018 ◽

Vol 9 ◽

pp. 133

Author(s):

Hastin Dian Anggraeni ◽

Margaretha Suharsini ◽

Ike Siti Indiarti ◽

Faraghea Yumasdhika

Keyword(s):

Pain Intensity ◽

Local Anesthetic ◽

Extraction Procedure ◽

Pain Scale ◽

Alpha Amylase ◽

Primary Tooth ◽

Amylase Level ◽

Salivary Alpha Amylase ◽

Local Anesthetic Injection ◽

Spearman’S Correlation

Objective: Several studies have tried to objectively assess pain measurements. The Wong-Baker Faces Pain Scale (WBFPS) is an instrument that is commonly used to assess pain intensity in children. This study aimed to analyze the correlation between the WBFPS and salivary alpha-amylase (SAA) level during a tooth extraction procedure with a local anesthetic injection in children aged 6–11 years.Methods: Twenty-five children aged 6–11 years who were to undergo the extraction of a primary tooth at the Dental and Oral Educational Hospital, Faculty of Dentistry Universitas Indonesia, were enrolled in this study. From all children, saliva was collected using a disposable saliva strip shortly after local anesthetic injection, and the SAA activity was then determined using a portable Nipro Cocoro Meter device. The WBFPS was measured at the same time. The correlation between the WBFPS and the SAA level was analyzed using Spearman’s correlation test. The statistically significant level was set at p≤0.05.Results: There was a significant correlation between the WBFPS and SAA level (p=0.002, r=0.581).Conclusion: Our data suggest that the SAA level might be a good index for objective pain intensity assessment.

Download Full-text

Evaluation of Changing Serum Blood Glucose Levels after Local Anesthetic Injection during Tooth Extraction

Journal of Dentomaxillofacial Radiology Pathology and Surgery ◽

10.18869/acadpub.3dj.4.2.15 ◽

2015 ◽

Vol 4 (2) ◽

pp. 15-19

Author(s):

Kamal Qaranizade ◽

Eshagh Lasemi ◽

Hamidreza Mahaseni Aghdam ◽

Farshid Malihi ◽

◽

...

Keyword(s):

Blood Glucose ◽

Local Anesthetic ◽

Tooth Extraction ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Local Anesthetic Injection

Download Full-text

Evaluation of changing Blood Glucose serum level After Local Anesthetic Injection During Tooth Extraction

AEA Randomized Controlled Trials ◽

10.1257/rct.445 ◽

2014 ◽

Author(s):

Hamidreza Mahaseni Aghdam

Keyword(s):

Blood Glucose ◽

Serum Level ◽

Local Anesthetic ◽

Tooth Extraction ◽

Local Anesthetic Injection

Download Full-text

Comparison of salivary alpha-amylase levels in gingivitis and periodontitis

Dental Journal (Majalah Kedokteran Gigi) ◽

10.20473/j.djmkg.v50.i4.p216-219 ◽

2017 ◽

Vol 50 (4) ◽

pp. 216

Author(s):

Dyah Nindita Carolina ◽

Yanti Rusyanti ◽

Agus Susanto

Keyword(s):

Periodontal Disease ◽

Inflammatory Disease ◽

Descriptive Study ◽

The Other ◽

Alpha Amylase ◽

Amylase Level ◽

Salivary Amylase ◽

Salivary Alpha Amylase ◽

The Difference ◽

Paired T Test

Background: The development of periodontal disease is influenced by bacteria-plaque, while there are also several factors modifying the host’s response, one of which is psychological stress. Alpha-amylase as a biomarker is also associated with periodontal inflammatory disease. Purpose: The purpose of this study was to examine the difference of alpha-amylase level between gingivitis and periodontitis. Methods: This research constitutes a descriptive study involving 44 subjects, divided into two groups: one of 22 gingivitis subjects and the other of 22 periodontitis subjects. These individuals completed a PSS-14 questionnaire before their levels of alpha salivary amylase were measured by Cocorometer. Data was analyzed by means of a paired T test and a Mann Whitney test with p < 0.05. Results: There were significant differences between the alpha-amylase levels of gingivitis and periodontitis. However, no significant contrast existed in the PSS-14 scores of the two periodontal disease groups. Conclusion: In conclusion alpha-amylase levels in the periodontitis group were higher than those in the gingivitis group and could be used as marker indicators of stress.

Download Full-text

Salivary Cortisol, Salivary Alpha Amylase, and the Dental Anxiety Scale

Anesthesia Progress ◽

10.2344/0003-3006-60.2.46 ◽

2013 ◽

Vol 60 (2) ◽

pp. 46-53 ◽

Cited By ~ 18

Author(s):

Hana Sadi ◽

Matthew Finkelman ◽

Morton Rosenberg

Keyword(s):

Salivary Cortisol ◽

Dental Anxiety ◽

Saliva Sample ◽

Anxiety Scale ◽

Alpha Amylase ◽

Cross Sectional ◽

Convenience Sample ◽

Salivary Alpha Amylase ◽

History Of ◽

Cross Sectional Design

AbstractThe aim of this study was to investigate the correlation between dental anxiety, salivary cortisol, and salivary alpha amylase (sAA) levels. Furthermore, the aim was to look into individual differences such as age, race, gender, any existing pain, or traumatic dental experience and their effect on dental anxiety. This study followed a cross-sectional design and included a convenience sample of 46. Every patient was asked to complete the Dental Anxiety Scale (DAS) and a basic demographic/dental history questionnaire. A saliva sample, utilizing the method of passive drooling, was then collected in 2-mL cryovials. Samples were analyzed for salivary cortisol and sAA levels by Salimetrics. Significant associations were observed between DAS scores and presence of pain and history of traumatic dental experience. However, no significant correlations were observed between DAS, cortisol, and sAA levels. Our study reconfirms that dental anxiety is associated with presence of pain and a history of traumatic dental experience. On the other hand, our study was the first to our knowledge to test the correlation between the DAS and sAA; nevertheless, our results failed to show any significant correlation between dental anxiety, cortisol, and sAA levels.

Download Full-text

In silico and in vitro studies of the inhibitory effect of antihistamine drug Cyproheptadine hydrochloride on human salivary alpha amylase.

Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry ◽

10.2174/1871523019666201023111825 ◽

2020 ◽

Vol 19 ◽

Author(s):

Benguechoua Madjda ◽

Benarous Khedidja ◽

Nia Samira ◽

Yousfi Mohamed

Keyword(s):

Molecular Docking ◽

Inhibitory Activity ◽

In Silico ◽

Diclofenac Sodium ◽

The Other ◽

Alpha Amylase ◽

Salivary Alpha Amylase ◽

Cyproheptadine Hydrochloride ◽

First Time

Background: For the first time, the inhibitory effects on human salivary alpha-amylase activity of the antiinflammatory drugs: indomethacin, diclofenac sodium, ketoprofen, diclofenac potassium, diclofenac, triamcinolone acetonide and the antihistamines drugs: levocetirizine dihydrochloride, desloratadine, cycloheptadine hydrochloride has been investigated to confirm the other properties of these drugs. Objective: This study aimed to determine the effect of nine known drugs on human salivary α-amylase in vitro and the nature of interactions with structure-activity relationship using molecular docking Method: The inhibition of human salivary alpha amylase by the six anti-inflammatory and three antihistamines drugs has been carried out using the new method that has been proved in our previous work. Molecular docking has been achieved for the first time for these drugs using AutoDock Vina program. Results: The Cyproheptadine hydrochloride presented the highest inhibitory activity against α-amylase with IC50=0.7 mg/ml, while the other drugs show weak activities (IC50 > 2 mg/ml). Conclusion: We conclude that Cyproheptadine hydrochloride and which studied by docking experiments exhibited the best inhibitory activity on salivary α-amylase in vitro & in silico.

Download Full-text