In silico and in vitro studies of the inhibitory effect of antihistamine drug Cyproheptadine hydrochloride on human salivary alpha amylase.
Background: For the first time, the inhibitory effects on human salivary alpha-amylase activity of the antiinflammatory drugs: indomethacin, diclofenac sodium, ketoprofen, diclofenac potassium, diclofenac, triamcinolone acetonide and the antihistamines drugs: levocetirizine dihydrochloride, desloratadine, cycloheptadine hydrochloride has been investigated to confirm the other properties of these drugs. Objective: This study aimed to determine the effect of nine known drugs on human salivary α-amylase in vitro and the nature of interactions with structure-activity relationship using molecular docking Method: The inhibition of human salivary alpha amylase by the six anti-inflammatory and three antihistamines drugs has been carried out using the new method that has been proved in our previous work. Molecular docking has been achieved for the first time for these drugs using AutoDock Vina program. Results: The Cyproheptadine hydrochloride presented the highest inhibitory activity against α-amylase with IC50=0.7 mg/ml, while the other drugs show weak activities (IC50 > 2 mg/ml). Conclusion: We conclude that Cyproheptadine hydrochloride and which studied by docking experiments exhibited the best inhibitory activity on salivary α-amylase in vitro & in silico.