scholarly journals COMBINED EFFECT OF SELECTIVE BIOACTIVE COMPOUNDS FROM PLANT ORIGIN IN AN ANIMAL MODEL OF ANXIETY

2020 ◽  
pp. 88-91
Author(s):  
SATHYA B. ◽  
BALAMURUGAN K. ◽  
ANBAZHAGAN S.
Keyword(s):  
Animal Model ◽  
Bioactive Compounds ◽  
Interactive Effect ◽  
Anxiolytic Effect ◽  
Plant Origin ◽  
Group I ◽  
Board Test ◽  
Objective Evidence ◽  
Single Compound ◽  
Hole Board Test

Objective: Evidence is emerging that specific combinations of bioactive compounds may be far more effective in protecting against several diseases as compared to the effect of a single compound. The present study was aimed to investigate the interactive effect of Diosgenin and Silymarin, the bioactive compounds from plant origin in an animal model of anxiety. Methods: Albino wistar rats of either sex were divided into five groups and treated for 5 d. Group I and II served as control and standard and test groups were treated with Diosgenin (100 mg/kg, p. o.), Silymarin (100 mg/kg, p. o.) and combination of Diosgenin (50 mg/kg, p. o.)+Silymarin (50 mg/kg, p. o.), respectively. Diazepam (1 mg/kg, i. p.) was used as a standard for the study. Anxiolytic effects were studied in the Elevated plus-maze, Hole-board test and Light/Dark model. Results: The results suggested that Diosgenin when given alone at a dose of 100 mg/kg, does not shown significant anxiolytic effect when as compared with control. Whereas, the compound Silymarin (100 mg/kg) shown significant anti-anxiety effect (P<0.01), independently. The same two bioactive compounds, given in combination at a dose of 50 mg/kg, (each), exhibited significant anxiolytic-like effect, potentially. Conclusion: It can be concluded that Diosgenin has got a synergistic effect on anti-anxiety action when given in combination with Silymarin.

Evaluation of anxiolytic and hypoglycemic potential of Cissus adnata Roxb. in animal model

2019 ◽  
Vol 17 (2) ◽  
Author(s):  
Mohammed Shamim Hasan ◽  
Md. Giash Uddin ◽  
Mohammed Shoibe ◽  
Abdullah Al Mahmud ◽  
Sujan Banik
Keyword(s):  
Glucose Tolerance Test ◽  
Methanolic Extract ◽  
Anxiolytic Effect ◽  
Tolerance Test ◽  
Mice Model ◽  
Board Test ◽  
Plus Maze ◽  
Hole Board ◽  
Phytochemical Constituents ◽  
Hole Board Test

AbstractBackgroundThis study was designed to evaluate the anxiolytic and hypoglycemic potential of methanolic extract of Cissus adnata Roxb. is a crucial medicinal plant used in many disorders belongs to Vitaceae family.MethodsElevated plus maze (EPM) test and hole board test was applied for the anxiolytic activity with the Swiss albino mice. The hypoglycemic activity was measured by the glucose tolerance test in mice model. The capacity to produce the desired effect of the plant extract (200 and 400 mg/kg) was compared with the anxiolytic drug of standard diazepam (1 mg/kg i.p.) and anti-diabetic drug glibenclamide (10 mg/kg i.p.), respectively.ResultsThe phytochemical screening of Cissus adnata extract exposed the presence of carbohydrate, phenol, flavonoid, saponins, cardiac glycoside, tannin, and gum. The anxiolytic effect was detected in both experiments which significantly raised the number of head dips and the time spent in the open arm of the EPM (p<0.05) as the dose enlarged. Hypoglycemic study of the extracts shows better effect by reducing blood glucose level.ConclusionsThe better anxiolytic and hypoglycemic activities in the present study are due to the existence of various phytochemical constituents like saponins, flavonoids, terpenoids, phenols, and tannins in this methanolic extract.

scholarly journals Neuropharmacological Evaluation of Methanolic Extract from Mercurialis Annua a Plant used in Moroccan Traditional Medicine

2017 ◽  
pp. 4-7
Author(s):  
Zouhra Doukkali ◽  
Mohamed Amine Boumahdi ◽  
EL Houcine Bouidida ◽  
Khalid Taghzouti
Keyword(s):  
Traditional Medicine ◽  
Methanolic Extract ◽  
Anxiolytic Effect ◽  
Traditional Use ◽  
Standard Drug ◽  
Board Test ◽  
Hole Board ◽  
Hole Board Test ◽  
Light Dark Box ◽  
Mercurialis Annua

Current therapeutic for the treatment of anxiety is associated with a wild variety of prominent side effects. The traditional use of plant extract to health care can indicate an important source of new pharmaceuticals. In Morocco traditional medicine, the use of Mercurialis annua is commonly recommended for relief of anxiety. Nevertheless, despite its popular use there are no studies related to its possible neuropharmacological effect. Here, we investigated the possible anxiolytic effect of the extract of M.annua after acute treatment in mice. The methanolic extract from the aerial parts of M.annua (100, 200 or 400 mg/kg) was orally administered, and its anxiolytic effect was evaluated in hole board test, the light–dark box test, and motor coordination with the rota rod test. Diazepam was employed as standard drug 1mg/kg. The methanolic extract of Ma 100 mg/kg increased the time spent in the brightly-lit chamber of the light/dark box, as well as in the number of times the animal crossed from one compartment to the other. Performance on the rota rod was unaffected. In the hole board test, the extract significantly increased head-dip counts. These results provides support for anxiolytic activity of Mercurialis annua, in line with its medicinal traditional use, and may also suggest a better side-effect profile of Mercurialis annua relative to diazepam.

scholarly journals Anxiolytic and hypnotic effects of Cocculus laurifolius leaf extract in mice

2019 ◽  
Vol 14 (1) ◽  
pp. 45-53
Author(s):  
Sidra Maqbool ◽  
Ishrat Younus
Keyword(s):  
Elevated Plus Maze ◽  
Ethanolic Extract ◽  
Anxiolytic Effect ◽  
Sodium Pentobarbital ◽  
Hypnotic Effect ◽  
Board Test ◽  
Plus Maze ◽  
Hole Board ◽  
Hole Board Test ◽  
Hypnotic Activity

The present study aims to assess the anxiolytic and hypnotic activity of ethanolic extract of Cocculus laurifolius leaf in mice. Anxiolytic activity was observed using behavioral paradigms while hypnotic activity was assessed by sodium pentobarbital induced hypnosis in mice. Animals were treated with the extract at dose 200 and 400 mg/kg orally for 30 days. Anxiolytic and hypnotic effect were observed on day 1, 7, 15 and 30 of treatment. Significant increase in the number of entries and time spent in open arm were observed in elevated plus maze. Similar results were observed in light/dark, open field and hole board test. The anxiolytic effect was substantially greater at dose 200 mg/kg in comparison to dose 400 mg/kg. Minimal hypnotic activity was observed with decrease in onset and prolongation in duration of sleep at dose 400 mg/kg, while dose 200 mg/kg did not produce any hypnotic effect. These results showed that the ethanolic extract of C. laurifolius leaf might have potential anxiolytic and hypnotic activity in mice.

Anti-depressant, anxiolytic and muscle relaxant activity of hydroalcoholic extract of Cissampelos pareira linn. Leaves

2021 ◽  
Vol 21 ◽  
Author(s):  
Mohammad Asif ◽  
Jayesh Dwivedi ◽  
Sandeep Yadav
Keyword(s):  
Muscle Relaxant ◽  
Anxiolytic Effect ◽  
Inclined Plane ◽  
Hydroalcoholic Extract ◽  
Elevated Plus Maze Test ◽  
Board Test ◽  
Plus Maze ◽  
Hole Board ◽  
Hole Board Test ◽  
Muscle Relaxant Activity

Background: The ethnopharmacological relevance suggests that the ethnic minorities of India use leaves of Cissampelos pareira L as a traditional medicine for curing various psychopharmacological disorders. Objective: To evaluate anti-depressant, anxiolytic, and muscle relaxant activity of hydroalcoholic extract of Cissampelos pareira. Results: No moribund status or mortality was observed in experimental mice up to 2000 mg/kg dose of Cissampelos pareira hydroalcoholic extract (CPHE). In the open field and actophotometer tests, CPHE 200 and 400 mg/kg treated mice with significantly abridged ambulation, a number of central squares crossed, total locomotion, and depicted less coordinated movements. While, in despair swim and tail suspension tests, CPHE 400 mg/kg treated mice significantly decreased duration of immobility and increased number of climbing, confirming its anti-depressant effect. In an elevated plus-maze test, CPHE 200 and 400 mg/kg increased the open arm exploration; in hole board test, CPHE 400 mg/kg treated rats augmented the number of head dips, depicting its anxiolytic effect. In rotarod, grip strength, and inclined plane test, CPHE 400 mg/kg treated mice decreased in fall off time on a rotating rod, suspended wire, or inclined plane. Furthermore, in the chimney test, treatment with CPHE 400 depicted less coordinated movements in mice, and mice of this group took more time to leave the cylinder, depicting its skeletal muscle relaxant effect. Conclusion: Based on the result, it can be concluded that CPHE 400 mg/kg exhibits strong anti-depressant, anxiolytic, and muscle relaxant effects, justifying its traditional uses.

scholarly journals Can the Hole–Board Test Predict a Rat’s Exploratory Behavior in a Free-Exploration Test?

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1068
Author(s):  
Wojciech Pisula ◽  
Klaudia Modlinska ◽  
Katarzyna Goncikowska ◽  
Anna Chrzanowska
Keyword(s):  
Exploratory Behavior ◽  
Predictive Value ◽  
Previous Experience ◽  
General Hypothesis ◽  
Free Exploration ◽  
Laboratory Rodents ◽  
Board Test ◽  
Motivational Mechanism ◽  
Hole Board ◽  
Hole Board Test

This study focuses on the rat activity in a hole–board setting that we considered a type of exploratory behavior. The general hypothesis is based on the claim that a motivational mechanism is central to both the response to novelty in a highly familiarized environment and the activity in the hole–board apparatus. Our sample consisted of 80 experimentally naive Lister Hooded rats. All rats were tested in the hole–board apparatus. Twenty individuals with the highest hole-board scores and twenty subjects with the lowest hole–board scores subsequently underwent an established free-exploration test. In our study, the scores obtained in the hole–board test had little predictive value for the rats’ activity in the free-exploration test. Based on our previous experience in studying exploratory behavior in the free-exploration test and the data presented in this paper, we suggest that the hole–board test is not an appropriate tool for measuring exploratory behavior in laboratory rodents.

Neuropharmacological Activity of Various Fractions Obtained from Solanum aethiopicum (Linn.) Fruit in Mice.

2020 ◽  
Vol 1 (5) ◽  
pp. 12-23
Author(s):  
A.R. Abubakar ◽  
◽  
I.H. Sani ◽  
S. Malami ◽  
A.H. Yaro ◽  
...  
Keyword(s):  
Open Field ◽  
Elevated Plus Maze ◽  
Toxicity Testing ◽  
Aqueous Fraction ◽  
Solanum Aethiopicum ◽  
Elevated Plus Maze Test ◽  
Board Test ◽  
Plus Maze ◽  
Hole Board ◽  
Hole Board Test

Background: Solanum aethiopicum (L.), family Solanaceae, is known as garden eggs. The fruit is used in the treatment of insomnia, diabetes and constipation. Objective: The aim of this study was to evaluate anxiolytic-like activity of fractions obtained from crude methanol extract of Solanum aethiopicum fruit. Method: Acute toxicity testing was conducted according to the OECD guidelines 420 via oral and intraperitoneal routes (ip). n-Hexane (HF), chloroform (CHF), ethyl-acetate (EAF), n-butanol (BF) and residual aqueous fraction (RAF) at doses of 25, 50 and 100 mg/kg ip were experimented using the open field, elevated plus maze, staircase, light dark box and hole-board tests. Results: Results: In open field test, there was statistically significant increase in frequency of central square entry by EAF 25mg/kg, 50mg/kg and 100mg/kg and RAF 25mg/kg, 50mg/kg and 100mg/kg all at p<0.05 compared to distilled water (D/W) group. Elevated plus maze test showed statistically significant increases in open arm entry and duration by CHF 25mg/kg, RAF 25mg/kg and 50mg/kg again at p<0.05. Also, in the staircase test, statistically significant decrease in frequency of rearing with no effect on step climbing was observed by RAF 25mg/kg (p< 0.05) compared to D/W. Light and dark box test produced increased light box entry and duration by EAF 25mg/kg, RAF 25mg/kg and 50mg/ kg at p<0.05. Furthermore, the hole -board test showed statistically significant increases in number of head dips by EAF 50mg/kg and 100mg/kg as well as RAF 25mg/kg, 50mg/kg and 100 mg/kg at p<0.05. Conclusion: The fractions obtained from Solanum aethiopicum fruits possesses anxiolytic-like activity.

Techniques to extract bioactive compounds from food by-products of plant origin

2012 ◽  
Vol 46 (2) ◽  
pp. 505-513 ◽  
Author(s):  
Hilde Wijngaard ◽  
Mohammad B. Hossain ◽  
Dilip K. Rai ◽  
Nigel Brunton
Keyword(s):  
Bioactive Compounds ◽  
Plant Origin ◽  
By Products

scholarly journals Anti-Inflammatory Effect of Ethanolic Extract of Carica Papaya Leaves and Indomethacin in Cotton Pellet Induced Granuloma in Animal Model

2019 ◽  
Vol 6 (1) ◽  
pp. 2-5 ◽  
Author(s):  
Afroza Sultana ◽  
Rumana Afroz ◽  
Onayza Yasmeen ◽  
Muqbula Tasrin Aktar ◽  
Md Abdullah Yusuf
Keyword(s):  
Animal Model ◽  
Carica Papaya ◽  
Ethanolic Extract ◽  
Granuloma Formation ◽  
Rat Paw Edema ◽  
Group I ◽  
Cotton Pellet ◽  
Anti Inflammatory ◽  
Group Ii ◽  
Inflammatory Effect

Background: Anti-inflammatory effect can be exerted by Carica papaya. Objective: The purpose of the present study was to see the anti-inflammatory effect of ethanolic extract of Carica papaya leaves and indomethacin in carrageenan induced rat paw edema animal model. Methodology: This was an animal study carried out in the Department of Pharmacology at Dhaka Medical College, Dhaka, Bangladesh during the period from July 2014 to June 2015 for a period of one (01) year. The leaves of Carica Papaya collected from Botanical garden, Mirpur, Dhaka, Bangladesh. The animals were divided into four groups. The animals were divided into four groups. In all the animals granuloma were induced by implantation of autoclaved cotton pellet on the 1st day and they treated as follows: Group I were served as control that was received normal saline. Group II were received ethanolic extract of Carica Papaya leaves. Group III were received ethanolic extract of Carica Papaya leaves. Group IV were received indomethacin. On the 15th day animals were anesthetized, implanted pellets were dissected out, dried at hit air oven and the final weights were measured. Result: The experiment was carried out on 48 Long Evan Norwegian rats. Rats were divided in 4 groups of six animals each. The mean increase of weight of cotton pellet was 48.06±1.02, 24.48±0.36, 22.42±0.56 and 18.02±0.43 in group I, II, III and IV respectively. The inhibition of granuloma formation was 49.06%, 53.35% and 62.51% in group II, III and IV respectively. Conclusion: In conclusion the ethanolic extract of Carica Papaya leaves has effects on cotton pellet induced granuloma formation in experimental rat. Journal of Current and Advance Medical Research 2019;6(1):2-5

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