scholarly journals EFFECT OF COMBINATION OF TWO PLANT EXTRACTS ON DIABETES MELLITUS

2018 ◽  
Vol 10 (4) ◽  
pp. 49
Author(s):  
Jose Deepa ◽  
N. A. Aleykutty ◽  
Harindran Jyoti
Keyword(s):  
Glucose Uptake ◽  
Plant Extracts ◽  
Ethanolic Extract ◽  
High Sensitivity ◽  
Psidium Guajava ◽  
Alpha Amylase ◽  
Individual Plant ◽  
Syzygium Cumini ◽  
Standard Drug

Objective: To investigate the anti-diabetic activity of combined ethanolic extracts (1:1mixture) of dry leaves of Syzygium cumini and Psidium guajava belonging to the family Myrtaceae as well as to compare the anti-diabetic activity of these plants by in vitro methods.Methods: In vitro glucose uptake assay was performed on cultured L6 cell lines (rat myoblast cell line) and estimated the glucose uptake using high sensitivity glucose oxidase kit. In vitro alpha amylase inhibitory assay was performed on porcine alpha amylase and the absorbance was measured at 540 nm using a microplate reader. Acarbose was used as the standard in both the methods.Results: At a concentration of 100µg/ml the percentage glucose uptake by the combined ethanolic extract (1:1 mixture) of Syzygium cumini and Psidium guajava leaves was 43.95 while for acarbose the corresponding value was 51.71. At 100 μg/ml the percentage of glucose uptake by Syzygium cumini and Psidium guajava was 27.62 and 22.17 respectively. The percentage inhibition of alpha amylase by the combined ethanolic extract (1:1 mixture) of Syzygium cumini and Psidium guajava leaves at a concentration of 1000 µg/ml was 36.51 and it was 29.26 for Syzygium cumini and 23.43 for Psidium guajava. For acarbose the percentage inhibition of alpha amylase was 73.82 at the concentration of 1000 µg/ml.Conclusion: The combined extract of the leaves of the plants selected was found to be more effective than individual plant extracts against diabetes. The percentage glucose uptake of the combined extract was found to be closer to that of the standard drug acarbose. On comparison of two plants Syzygium cumini was found to be more active against diabetes than Psidium guajava. As the 1:1 mixture of the ethanolic extract is found to be more active, the combination of the two plants can be used to formulate drugs for treating diabetes.

scholarly journals IN VITRO ASSESSMENT OF ANTHELMINTIC AND ALPHA-AMYLASE INHIBITION OF SCHLEICHERA OLEOSA (LOUR.) OKEN LEAF EXTRACTS

2018 ◽  
Vol 11 (9) ◽  
pp. 487
Author(s):  
Shambaditya Goswami ◽  
Ravindra Pal Singh
Keyword(s):  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Alpha Amylase ◽  
Control Treatment ◽  
Aqueous Extracts ◽  
Leaf Extracts ◽  
Standard Drug ◽  
Isolation And Characterization ◽  
Schleichera Oleosa

Objective: The present study deals with the effects of Schleichera oleosa (Lour.) Oken leaf extracts on helminths and alpha-amylase inhibition. Identification of phytochemicals and physicochemical analysis were also performed.Methods: Different concentrations (25, 50, and 100 mg/ml) of petroleum ether, acetone, chloroform, ethanol, and aqueous extracts of the leaf were used to examine the effects. For the evaluation of in vitro anthelmintic activity, several earthworms (Eisenia fetida, Perionyx excavates, and Pheretima posthuma) and nematode (Ascaridia galli) were taken, while albendazole was used as a standard drug and Tween 80 (3%) in normal saline (0.9% NaCl) was considered as a control treatment. In vitro alpha-amylase inhibition of different extracts (10–100 mg/ml) was done spectrophotometrically by dinitrosalicylic acid - starch azure method.Results: The ethanolic extract showed the maximum presence of phytochemicals among all the extracts, which included alkaloids, tannins, flavonoids, saponin glycosides, phenolic compounds, resins, and amino acids. The outcomes of the determination of physicochemical parameters and fluorescence characters provided the satisfactory results. Significant anthelmintic activity was established by the ethanolic and aqueous extracts of the leaf among all the extracts and the responses, so observed, were dose responsive. Inhibition of alpha-amylase by ethanolic and aqueous extracts was significant with the IC50 value of 36.63 and 73.94 μg/ml, respectively, when compared to standard acarbose.Conclusion: The ethanolic extract was the more potent candidate for both the effects, and the effect of extract was best against A. galli, P. posthuma, and E. fetida at higher concentration. Isolation and characterization of therapeutic constituents would be the future interest.

scholarly journals Anti-hyperglycaemic and Mode of Action of Thaumatococcus danielli (BENN.) BENTH Ethanol Leave Extract in Streptozotocin-induced Diabetic Rats

2019 ◽  
pp. 1-10
Author(s):  
Folorunsho A. Ajayi ◽  
Olubukola. S. Olorunnisola ◽  
Adewale. Adetutu ◽  
Folashade G. Olorunfemi ◽  
Abiodun O. Owoade ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Glucose Uptake ◽  
Mode Of Action ◽  
Inhibitory Effect ◽  
Yeast Cells ◽  
Alpha Amylase ◽  
Standard Drug ◽  
Uptake Inhibition ◽  
Enzymatic Glycosylation

Thaumatococcus danielli (Benn.) Benth, a member of the Maranthaceae family has continued to be of immense benefit to the people in the tropics especially in Nigeria. The leaf is widely used among the “Yoruba’s” as a wrapping leaf and for the management of diabetes mellitus. Aim: This study, evaluated the anti-diabetic and possible mode of action(s) of ethanol leaves extract of Thaumatococcus danielli using in vivo and in vitro approach. Methods: Diabetes was induced in Albino rats by administration of Streptozotocin (65 mg/kg/b.wt, i.p). The ethanol leave extract of Thaumatococcus danielli (at a dose of 250 mg/kg and 500 mg/kg body weight was administered at single dose per day to diabetes induced rats for a period of 14 days. The possible mode of action of extract was assessed through in vitro inhibitory effect on alpha amylase, non-enzymatic glycosylation of haemoglobin and glucose uptake in yeast cell. Results: The results showed that the plant extracts demonstrated dose and time dependent reduction in blood glucose. The extract at 250 mg/kg /b.wt and 500 mg/kg/b.wt caused a significant percentage reduction (35.00%/42.04% and 42.16%/60.43%) in blood glucose when compared with the group treated with (25 mg/kg/b.wt) of the standard drug (30.51/40.88%) and the diabetic control (10.46%/-13.67%) on day 7 and day 14 respectively. Although, the extract demonstrated significant (p<0.05) dose dependent inhibitory effect on alpha amylase with an IC50 of 837.97 µg/ml, its activity was significantly (P<0.05) lower than the standard Acarbose. Conversely, the extract showed stronger inhibition of non-enzymatic glycosylation of haemoglobin (87.51%) and enhance glucose uptake in yeast cells by 85.56% when compared with the standard drug Trolax and Metronidazole respectively. Conclusion: The results of this study revealed that Thaumatococcus danielli (Benth) leaves contain anti-hyperglycaemic agent (s) and its possible mode of action is by promoting glucose uptake, inhibition of non-enzymatic glycosylation of haemoglobin and alpha amylase activity.

scholarly journals Anti-diabetic activity of Clerodendrum paniculatum leaves by In-vitro, In-vivo and Ex-vivo methods

2021 ◽  
Vol 16 (1) ◽  
pp. 211-118
Author(s):  
Venkatesh S ◽  
Aswani K ◽  
Asheena Asharaf V V ◽  
Anjitha P ◽  
Suresh A ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Ex Vivo ◽  
Soxhlet Extraction ◽  
Chloroform Extract ◽  
Alpha Amylase ◽  
Standard Drug ◽  
Ic50 Values ◽  
Alpha Glucosidase

The present study has been undertaken to evaluate the in-vitro, in-vivo and ex-vivo anti-diabetic activity of leaves of chloroform extract Clerodendrum paniculatum (CECP). The extract was prepared by soxhlet extraction. Phytochemical screening indicates the presence of flavonoids, phenols, carbohydrates etc. The anti-diabetic activity of extract was studied by in-vitro (alpha amylase inhibition and alpha glucosidase inhibition assay), in-vivo (streptozotocin induced diabetes) and ex-vivo (glucose uptake by rat hemi - diaphragm method). For in-vitro studies, the inhibitory action of CECP was compared with standard drug Acarbose. The IC50 values of CECP for alpha amylase and alpha glucosidase was found to be 158.396 µg/ml and 113.122 µg/ml respectively and the extract shows significant anti-diabetic activity. For in-vivo and ex-vivo studies Glibenclamide was used as a standard drug to compare the blood glucose level and uptake of glucose was calculated. The results obtained from the study indicate that both 200 mg/kg and 400 mg/kg of CECP showed significant anti-diabetic activity. The 400 mg/kg of CECP showed better activity when compared to 200 mg/kg of the extract. The glucose uptake study was performed by isolated rat hemidiaphragm method. The hemi diaphragm obtained from the rats treated with both the doses of CECP showed significant glucose uptake.

scholarly journals In silico and in vitro assessment on antidiabetic efficacy of secondary metabolites from Syzygium cumini (L.) Skeels

2016 ◽  
Vol 3 (4) ◽  
pp. 360
Author(s):  
Twinkle Sunder Bansode ◽  
Amit Gupta ◽  
B K Salalkar
Keyword(s):  
Secondary Metabolites ◽  
Ellagic Acid ◽  
In Silico ◽  
Ethanol Extract ◽  
Bioactive Compound ◽  
Alpha Amylase ◽  
Herbal Remedies ◽  
Syzygium Cumini ◽  
Standard Drug

India ranks high for prevalence of diabetes and the treatment of diabetes without any side effects is still challenging. Though herbal remedies help reduce the side effect, proper standardization of phytochemical which prove as a bioactive compound, its proper dose and clinical trials are lacking. In our investigation, we studied the binding mechanism of the secondary metabolites of Syzygium cumini, their in vitro antidiabetic activity and the number of phytochemicals present. In silico study revealed that ellagic acid has a potential to modulate the carbohydrate metabolizing enzyme activity showing higher affinity for the enzymes with much lesser binding energy, -4.73 kcal/mol for alpha amylase, -4.87 kcal/mol for beta-glucosidase, -4.79 kcal/mol for glycogen synthase kinase, -4.18 kcal/mol for glucokinase and -4.49 kcal/mol for alpha-glucosidase. In vitro-Alpha amylase inhibitory activity assay showed that ethanol extract has the highest value of percent inhibition (73.33%) as compared to standard drug Acarbose (65.99%). Finally, TLC analysis cleared that ethanol extract contains five compounds one of which may be a bioactive compound, ellagic acid. Further purification and characterization of the ellagic acid is needed.

In vitro glucose uptake activity of Aegles marmelos and Syzygium cumini by activation of Glut-4, PI3 kinase and PPARγ in L6 myotubes

Phytomedicine ◽  
2006 ◽  
Vol 13 (6) ◽  
pp. 434-441 ◽  
Author(s):  
R. Anandharajan ◽  
S. Jaiganesh ◽  
N.P. Shankernarayanan ◽  
R.A. Viswakarma ◽  
A. Balakrishnan
Keyword(s):  
Glucose Uptake ◽  
Pi3 Kinase ◽  
Syzygium Cumini ◽  
Glut 4 ◽  
L6 Myotubes ◽  
Uptake Activity

IN VITRO HYPOGLYCEMIC EFFECTS OF CAESALPINIA BONDUCELLA AND MYRISTICA FRAGRANS SEED EXTRACTS

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (02) ◽  
pp. 57-62
Author(s):  
M. A Bhutkar ◽  
◽  
S. D Bhinge ◽  
D. S. Randive ◽  
G. H Wadkar ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Plant Extracts ◽  
Glucose Concentration ◽  
Yeast Cells ◽  
In Vivo Models ◽  
Myristica Fragrans ◽  
Glucose Diffusion ◽  
Caesalpinia Bonducella

The present investigation was undertaken to assess the hypoglycemic potential of Caesalpinia bonducella (C.bonducella) and Myristica fragrans (M.fragrans), employing various in vitro techniques. The extracts of seeds of C. bonducella and M. fragrans were studied for their effects on glucose adsorption capacity, in vitro glucose diffusion, in vitro amylolysis kinetics and glucose transport across the yeast cells. It was observed that the plant extracts under study adsorbed glucose and the adsorption of glucose increased remarkably with an increase in glucose concentration. There were no significant (p≤0.05) differences between their adsorption capacities. The results of amylolysis kinetic experimental model revealed that the rate of glucose diffusion was found to be increased with time from 30 to 180 min and both the plant extracts demonstrated significant inhibitory effects on movement of glucose into external solution across dialysis membrane as compared to control. Also, the plant extracts promoted glucose uptake by the yeast cells. It was observed that the enhancement of glucose uptake was dependent on both the sample and glucose concentration. C. bonducella extract exhibited significantly higher (p≤0.05) activity than the extract of M. fragrans at all concentrations. The results of the study verified the hypoglycemic activity of the extracts of C. bonducella and M. fragrans. However, the observed effects exhibited by the extracts of seeds of C. bonducella and M. fragrans need to be confirmed by using different in vivo models and clinical trials for their effective utilization as therapeutic agents in better management of diabetes mellitus.

scholarly journals In vitro antioxidant activity of Psidium guajava Linn. by using ethanolic extract fraction of leaves and bark

2018 ◽  
Vol 02 (01) ◽  
Author(s):  
Subba Rao Ch ◽  
Arun Kumar S ◽  
Javvad F Ali ◽  
Priyadarshini P ◽  
Hinduja M ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethanolic Extract ◽  
Psidium Guajava ◽  
Extract Fraction ◽  
In Vitro Antioxidant Activity

scholarly journals Thrombolytic, CNS Depressant and Anti-Diarrhoeal Activities of Ethanolic Extract of Bark of Syzygium cumini L. Skeels: An In-Vivo and In-Vitro Study

2018 ◽  
Vol 8 (3) ◽  
pp. 129-136
Author(s):  
Fokhrul Abedin ◽  
Md. Saddam Hussain ◽  
Areful Islam ◽  
Niloy Sen ◽  
Abhijit Das ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Ethanolic Extract ◽  
Vitro Study ◽  
Syzygium Cumini ◽  
Cns Depressant

scholarly journals Ethanolic extract of ocimum kilimandscharicum linnleaves: phytochemical and in vitro pharmacological activity

2021 ◽  
pp. 106-109
Author(s):  
Yamini N ◽  
Lahari S ◽  
Phani deepthi V
Keyword(s):  
In Vitro Model ◽  
Ethanolic Extract ◽  
Clinical Development ◽  
Dependent Manner ◽  
Thrombolytic Activity ◽  
Standard Drug ◽  
Ethanolic Extracts ◽  
Vitro Model ◽  
Dose Dependent

Using an in vitro model, the anti-thrombolytic efficacy of ethanolic extracts of Ocimum kilimandscharicum Linn was investigated. The researchers discovered that different concentrations of the extract had significant anti-thrombolytic activity in a dose-dependent manner , which was comparable to a standard drug. As a result of the presence of flavonoids and polyphenols in the plant extract, it can be concluded that it has a promising future in the treatment of thrombosis. This knowledge will be useful in the clinical development of thrombolytic therapeutics by identifying more potent anti-thrombolytic principles from natural resources..    

scholarly journals Atividade antimicrobiana in vitro dos extratos aquosos, hidroalcoólicos e alcoólicos das folhas de espécies da família Myrtaceae frente à cepas de bactérias de interesse

2017 ◽  
Vol 16 (2) ◽  
pp. 139
Author(s):  
Francisca Hérica Chaves Albuquerque ◽  
Karina Da Silva Soares ◽  
Maria Auxiliadora Silva Oliveira
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Psidium Guajava ◽  
Syzygium Cumini ◽  
Eugenia Uniflora ◽  
Mueller Hinton

<p><strong>Introdução</strong>: a Organização Mundial da Saúde (OMS) tem incentivado o estudo de plantas tradicionalmente conhecidas como medicinais, tendo como objetivo avaliar cientificamente os benefícios da utilização de medicamentos fitoterápicos e de conhecer, ao mesmo tempo, a segurança de seu uso indevido. <strong>Objetivos</strong>: este trabalho teve como objetivo avaliar a atividade antimicrobiana do extrato aquoso, hidroalcoólico e alcoólico de folhas de espécies da família Myrtaceae frente às cepas de bactérias de interesse. <strong>Metodologia</strong>: a análise da atividade antibacteriana dos extratos das folhas das plantas, jambo, azeitona preta, pitanga e goiaba foram verificados a partir do teste de difusão em Ágar Mueller-Hinton. As cepas utilizadas, após reativação, foram diluídas em solução salina estéril a 0,85%. Sendo consideradas com potencial antimicrobiano aqueles extratos que geraram halos ≥ 7 mm (sete milímetros). <strong>Resultados</strong>: os resultados encontrados na análise dos dados do extrato aquoso demonstraram que Eugenia uniflora, Syzygium cumini e Psidium guajava apresentaram atividade antimicrobiana sobre Staphylococcus aureus, na concentração de 100 mg/mL. Na análise dos dados dos extratos hidroalcoólico, Syzygium cumini demonstrou atividade antimicrobiana na concentração de 100 mg/mL sobre Pseudomonas aeruginosa, diante de Staphylococcus aureus, Eugenia uniflora, Syzygium malaccense, Syzygium cumini e Psidium guajava demonstraram atividade na concentração de 100 mg/mL cada. Observando os resultados dos extratos alcoólicos, Psidium guajava e Syzygium cumini demonstraram atividade antimicrobiana diante das quatro cepas estudadas. <strong>Conclusão</strong>: o presente estudo demonstrou que os extratos alcoólicos das espécies em estudo apresentaram melhor atividade antimicrobiana e maior espectro de ação quando comparado ao extrato aquoso e hidroalcoólico.</p>

Sign in / Sign up

Export Citation Format

Share Document