Carnosine increases efficiency of temozolomide and irradiation treatment of isocitrate dehydrogenase-wildtype glioblastoma cells in culture

2019 ◽  
Vol 15 (32) ◽  
pp. 3683-3691
Author(s):  
Johannes Dietterle ◽  
Henry Oppermann ◽  
Annegret Glasow ◽  
Karsten Neumann ◽  
Jürgen Meixensberger ◽  
...  
Keyword(s):  
Side Effects ◽  
Cell Cultures ◽  
Isocitrate Dehydrogenase ◽  
Standard Therapy ◽  
Primary Cell ◽  
Ionizing Irradiation ◽  
Cells In Culture ◽  
Naturally Occurring ◽  
Irradiation Treatment ◽  
Different Doses

Aim: The naturally occurring dipeptide carnosine (CAR) has been considered for glioblastoma therapy. As CAR also protects against ionizing irradiation (IR), we investigated whether it may counteract standard therapy consisting of postsurgery IR and treatment with temozolomide (TMZ). Materials & methods: Four isocitrate dehydrogenase-wildtype primary cell cultures were exposed to different doses of IR and different concentrations of TMZ and CAR. After exposure, viability under the different conditions and combinations of them was determined. Results: All cultures responded to treatment with TMZ and IR with reduced viability. CAR further decreased viability when TMZ and IR were combined. Conclusion: Treatment with CAR does not counteract glioblastoma standard therapy. As the dipeptide also protects nontumor cells from IR, it may reduce deleterious side effects of treatment.

Synergistic action of propolis with levodopa in the management of Parkinsonism in Drosophila melanogaster

2020 ◽  
Vol 17 (3) ◽  
Author(s):  
Emmanuel Tiyo Ayikobua ◽  
Josephine Kasolo ◽  
Keneth Iceland Kasozi ◽  
Ejike Daniel Eze ◽  
Abass Safiriyu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Drosophila Melanogaster ◽  
Side Effects ◽  
Motor Activity ◽  
Ethanolic Extract ◽  
Synergistic Action ◽  
Content Type ◽  
Treatment Groups ◽  
Ethanolic Extract Of Propolis ◽  
Different Doses

AbstractBackgroundThe Phosphatase and tensin-induced putative kinase 1 (PINK1B9) mutant for Drosophila melanogaster is a key tool that has been used in assessing the pathology of Parkinsonism and its possible remedy. This research was targeted toward determining the effects of ethanolic extract of propolis, with levodopa therapy in the management of Parkinsonism.MethodThe PINK1B9 flies were divided into groups and fed with the different treatment doses of ethanoic extract of propolis. The treatment groups were subjected to 21 days of administration of propolis and the levodopa at different doses after which percentage climbing index, antioxidant activity and lifespan studies were done.ResultsPropolis alone improved motor activity, antioxidant and lifespan in Drosophila melanogaster than in PINK1 flies. Propolis in combination with levodopa significantly (P<0.05) improved physiological parameters at higher than lower concentrations in Parkinsonism Drosophila melanogaster demonstrating its importance in managing side effects associated with levodopa.ConclusionPropolis is a novel candidate as an alternative and integrative medicinal option to use in the management of Parkinsonism in both animals and humans at higher concentrations.

The Effect of a Single Dose of Quazepam (Sch-16134) on the Sleep of Chronic Insomniacs

1979 ◽  
Vol 7 (6) ◽  
pp. 583-587 ◽  
Author(s):  
Thomas Roth ◽  
Elizabeth I Tietz ◽  
Milton Kramer ◽  
Mark Kaffeman
Keyword(s):  
Single Dose ◽  
Side Effects ◽  
Chronic Administration ◽  
Sleep Efficiency ◽  
Objective Measures ◽  
Subjective Measures ◽  
Sleep Maintenance ◽  
Eeg Changes ◽  
Different Doses ◽  
Polysomnographic Recording

The present study evaluated the efficacy of 25 mg of quazepam, a new benzodiazepine hypnotic, in a population of chronic insomniacs. The results indicate that a single dose (25 mg) administered for one night was efficacious when measured both objectively by polysomnographic recording and subjectively by questionnaire with no reported side-effects. The change in the objective measures paralleled the direction of change in subjective measures. Sleep efficiency and sleep maintenance were improved without EEG changes in Stages 2, 3-4, and REM. Further study is needed to evaluate the effects of chronic administration of different doses of quazepam in chronic insomniacs.

scholarly journals Author Correction: Novel methods to establish whole‑body primary cell cultures for the cnidarians Nematostella vectensis and Pocillopora damicornis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
James D. Nowotny ◽  
Michael T. Connelly ◽  
Nikki Traylor‑Knowles
Keyword(s):  
Cell Cultures ◽  
Primary Cell ◽  
Whole Body ◽  
Nematostella Vectensis ◽  
Pocillopora Damicornis ◽  
Primary Cell Cultures ◽  
Novel Methods

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Detection and quantification of farnesol-induced apoptosis in difficult primary cell cultures by TaqMan protein assay

APOPTOSIS ◽  
2013 ◽  
Vol 18 (4) ◽  
pp. 452-466 ◽  
Author(s):  
Christina Pfister ◽  
Heike Pfrommer ◽  
Marcos S. Tatagiba ◽  
Florian Roser
Keyword(s):  
Cell Cultures ◽  
Primary Cell ◽  
Protein Assay ◽  
Primary Cell Cultures ◽  
Induced Apoptosis ◽  
Detection And Quantification

scholarly journals Evidence for reduced Cl- and increased Na+ permeability in cystic fibrosis human primary cell cultures.

1988 ◽  
Vol 405 (1) ◽  
pp. 77-103 ◽  
Author(s):  
R C Boucher ◽  
C U Cotton ◽  
J T Gatzy ◽  
M R Knowles ◽  
J R Yankaskas
Keyword(s):  
Cystic Fibrosis ◽  
Cell Cultures ◽  
Primary Cell ◽  
Primary Cell Cultures ◽  
Na Permeability

Myogenesis in primary cell cultures from drosophila melanogaster: Protein synthesis and actin heterogeneity during development

Cell ◽  
1978 ◽  
Vol 13 (4) ◽  
pp. 589-598 ◽  
Author(s):  
Robert V. Storti ◽  
Sharon J. Horovitch ◽  
Matthew P. Scott ◽  
Alexander Rich ◽  
Mary Lou Pardue
Keyword(s):  
Protein Synthesis ◽  
Drosophila Melanogaster ◽  
Cell Cultures ◽  
Primary Cell ◽  
Primary Cell Cultures

Primary cell cultures from sea urchin ovaries: a new experimental tool

2013 ◽  
Vol 50 (2) ◽  
pp. 139-145 ◽  
Author(s):  
Silvia Mercurio ◽  
Cristiano Di Benedetto ◽  
Michela Sugni ◽  
M. Daniela Candia Carnevali
Keyword(s):  
Sea Urchin ◽  
Cell Cultures ◽  
Primary Cell ◽  
Primary Cell Cultures ◽  
Experimental Tool

Improved attachment and spreading in primary cell cultures of the eastern oyster, Crassostrea virginica

1999 ◽  
Vol 35 (10) ◽  
pp. 593-598 ◽  
Author(s):  
John T. Buchanan ◽  
Jerome F. La Peyre ◽  
Richard K. Cooper ◽  
Terrence R. Tiersch
Keyword(s):  
Crassostrea Virginica ◽  
Cell Cultures ◽  
Eastern Oyster ◽  
Primary Cell ◽  
Primary Cell Cultures
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