The virome of bronchoalveolar lavage fluid from patients with fever of unknown origin

Background: Viral metagenomics, a high-throughput sequencing combined with virus sequence-independent amplification by random PCR, allows for unbiased detection of virtually any viruses present in samples. Materials & methods: In order to investigate the virome of bronchoalveolar lavage fluid from patients with fever of unknown origin, 58 samples collected from diseased patients were characterized and compared. Results: Some representatives of Anelloviridae were identified, we found the torque teno virus (TTV) accounts for the majority of virus communities and were more prevalent in the specimens of febrile patients. Phylogenetic analysis suggested that these anellovirus isolates were close to the previous TTV available in GenBank®. Conclusion: All these data indicate that the human anellovirus species TTV may associated with fever of unknown origin.

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High-throughput sequencing of 16S rDNA amplicons characterizes bacterial composition in bronchoalveolar lavage fluid in patients with ventilator-associated pneumonia

Drug Design Development and Therapy ◽

10.2147/dddt.s87634 ◽

2015 ◽

pp. 4883

Author(s):

Shu-Feng Zhou ◽

Xiao-Jun Yang ◽

Yan-Bo Wang ◽

Zhi-Wei Zhou ◽

Guo-Wei Wang ◽

...

Keyword(s):

Bronchoalveolar Lavage ◽

16S Rdna ◽

High Throughput ◽

Bronchoalveolar Lavage Fluid ◽

High Throughput Sequencing ◽

Lavage Fluid ◽

Ventilator Associated Pneumonia ◽

Bacterial Composition

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Identification of neutrophil chemotactic factors in bronchoalveolar lavage fluid of asthmatic patients

Clinical & Experimental Allergy ◽

10.1046/j.1365-2222.1997.1330817.x ◽

1997 ◽

Vol 27 (4) ◽

pp. 396-405 ◽

Cited By ~ 7

Author(s):

L. M. TERAN ◽

M. G. CAMPOS ◽

B. T. BEGISHVILLI ◽

J.-M. SCHRODER ◽

R. DJUKANOVIC ◽

...

Keyword(s):

Bronchoalveolar Lavage ◽

Bronchoalveolar Lavage Fluid ◽

Lavage Fluid ◽

Asthmatic Patients ◽

Chemotactic Factors

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A comparison of lung surfactant protein A (SP-A) from human bronchoalveolar lavage fluid and synovial fluid

Immunology Letters ◽

10.1016/s0165-2478(97)87286-0 ◽

1997 ◽

Vol 56 (1-3) ◽

pp. 112-113

Author(s):

T Hickling

Keyword(s):

Synovial Fluid ◽

Bronchoalveolar Lavage ◽

Bronchoalveolar Lavage Fluid ◽

Lung Surfactant ◽

Protein A ◽

Lavage Fluid ◽

Surfactant Protein ◽

Surfactant Protein A ◽

Lung Surfactant Protein

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Faculty Opinions recommendation of Bronchoalveolar lavage fluid galactomannan for the diagnosis of invasive pulmonary aspergillosis in patients with hematologic diseases.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1282063.749160 ◽

2009 ◽

Author(s):

David Andes

Keyword(s):

Bronchoalveolar Lavage ◽

Bronchoalveolar Lavage Fluid ◽

Invasive Pulmonary Aspergillosis ◽

Lavage Fluid ◽

Pulmonary Aspergillosis ◽

Hematologic Diseases

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Faculty Opinions recommendation of Early mannan detection in bronchoalveolar lavage fluid with preemptive treatment reduces the incidence of invasive Candida infections in preterm infants.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.3557958.3264056 ◽

2010 ◽

Author(s):

David Andes

Keyword(s):

Bronchoalveolar Lavage ◽

Preterm Infants ◽

Bronchoalveolar Lavage Fluid ◽

Lavage Fluid ◽

Preemptive Treatment ◽

Candida Infections

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Faculty Opinions recommendation of Increased MCP-1, RANTES, and MIP-1alpha in bronchoalveolar lavage fluid of allergic asthmatic patients.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717970980.793469373 ◽

2013 ◽

Author(s):

Achsah Keegan

Keyword(s):

Bronchoalveolar Lavage ◽

Bronchoalveolar Lavage Fluid ◽

Lavage Fluid ◽

Asthmatic Patients ◽

Allergic Asthmatic

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Sensitive Quantification of Nicotine in Bronchoalveolar Lavage Fluid by Acetone Precipitation Combined With Isotope-Dilution Liquid Chromatography-Tandem Mass Spectrometry

ACS Omega ◽

10.1021/acsomega.0c05696 ◽

2021 ◽

Author(s):

Baoyun Xia ◽

Benjamin C. Blount ◽

Lanqing Wang

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Tandem Mass Spectrometry ◽

Bronchoalveolar Lavage ◽

Isotope Dilution ◽

Bronchoalveolar Lavage Fluid ◽

Lavage Fluid ◽

Tandem Mass ◽

Chromatography Tandem Mass Spectrometry ◽

Liquid Chromatography Tandem Mass

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Treatment with senicapoc in a porcine model of acute respiratory distress syndrome

Intensive Care Medicine Experimental ◽

10.1186/s40635-021-00381-z ◽

2021 ◽

Vol 9 (1) ◽

Author(s):

Asbjørn G. Petersen ◽

Peter C. Lind ◽

Anne-Sophie B. Jensen ◽

Mark A. Eggertsen ◽

Asger Granfeldt ◽

...

Keyword(s):

Acute Respiratory Distress Syndrome ◽

Lung Injury ◽

Respiratory Distress Syndrome ◽

Respiratory Distress ◽

Bronchoalveolar Lavage ◽

Primary Endpoint ◽

Bronchoalveolar Lavage Fluid ◽

Distress Syndrome ◽

Pulmonary Hemorrhage ◽

Lavage Fluid

Abstract Background Senicapoc is a potent and selective blocker of KCa3.1, a calcium-activated potassium channel of intermediate conductance. In the present study, we investigated whether there is a beneficial effect of senicapoc in a large animal model of acute respiratory distress syndrome (ARDS). The primary end point was the PaO2/FiO2 ratio. Methods ARDS was induced in female pigs (42–49 kg) by repeated lung lavages followed by injurious mechanical ventilation. Animals were then randomly assigned to vehicle (n = 9) or intravenous senicapoc (10 mg, n = 9) and received lung-protective ventilation for 6 h. Results Final senicapoc plasma concentrations were 67 ± 18 nM (n = 9). Senicapoc failed to change the primary endpoint PaO2/FiO2 ratio (senicapoc, 133 ± 23 mmHg; vehicle, 149 ± 68 mmHg). Lung compliance remained similar in the two groups. Senicapoc reduced the level of white blood cells and neutrophils, while the proinflammatory cytokines TNFα, IL-1β, and IL-6 in the bronchoalveolar lavage fluid were unaltered 6 h after induction of the lung injury. Senicapoc-treatment reduced the level of neutrophils in the alveolar space but with no difference between groups in the cumulative lung injury score. Histological analysis of pulmonary hemorrhage indicated a positive effect of senicapoc on alveolar–capillary barrier function, but this was not supported by measurements of albumin content and total protein in the bronchoalveolar lavage fluid. Conclusions In summary, senicapoc failed to improve the primary endpoint PaO2/FiO2 ratio, but reduced pulmonary hemorrhage and the influx of neutrophils into the lung. These findings open the perspective that blocking KCa3.1 channels is a potential treatment to reduce alveolar neutrophil accumulation and improve long-term outcome in ARDS.

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