Seroprevalence of the hepatitis E virus antibodies among blood donors in the Qassim region, Saudi Arabia

2021 ◽  
Author(s):  
Bader Y Alhatlani ◽  
Waleed A Aljabr ◽  
Mohammed S Almarzouqi ◽  
Sami M Alhatlani ◽  
Rayan N Alzunaydi ◽  
...  
Keyword(s):  
Public Health ◽  
Saudi Arabia ◽  
Blood Transfusion ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Indirect Elisa ◽  
Hepatitis E ◽  
Public Health Issue ◽  
Serum Samples ◽  
Major Public Health Issue

Aim: Hepatitis E virus (HEV) transmission through blood transfusion is a major public health issue worldwide. We aimed to determine the seroprevalence of HEV in blood donors in the Qassim region of Saudi Arabia. Materials & methods: Serum samples (n = 1078) were collected from volunteer blood donors and tested for the presence of anti-HEV IgG and IgM by indirect ELISA. Results: The seroprevalence of anti-HEV IgG among the blood donors was 5.7% overall. Anti-HEV IgG and IgM seropositivity were significantly higher in non-Saudi donors than in Saudi donors (22.1 vs 3 and 7.8 vs 0.2% for anti-HEV IgG and IgM, respectively). Conclusion: The seroprevalence of HEV among blood donors in the Qassim region was lower than previous estimates for other regions of the country and neighboring countries.

scholarly journals Seroprevalence of the hepatitis E virus among blood donors in the Qassim Region, Saudi Arabia

2020 ◽  
Author(s):  
Bader Alhatlani ◽  
Waleed Aljabr ◽  
Mohammed Almarzouqi ◽  
Sami Alhatlani ◽  
Rayan Alzunaydi ◽  
...  
Keyword(s):  
Public Health ◽  
Saudi Arabia ◽  
Blood Transfusion ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Geographical Area ◽  
Hepatitis E ◽  
Public Health Issue ◽  
Serum Samples ◽  
Major Public Health Issue

Abstract Objectives The aim of this study was to evaluate the seroprevalence of hepatitis E virus (HEV), a major public health issue worldwide with the potential for transmission via blood transfusion, in blood donors in the Qassim Region, Saudi Arabia. Serum samples (n = 1,078) were collected from volunteer blood donors from January to April 2019 and tested for the presence of anti-HEV IgG and IgM by indirect enzyme-linked immunosorbent assays. Results Overall, the seroprevalence of anti-HEV IgG and IgM among blood donors was 5.7% and 1.3%, respectively. Additionally, the seropositive rates of anti-HEV IgG and IgM were significantly higher in non-Saudi donors (22.1% and 7.8%) than in Saudi donors (3% and 0.2%). The seroprevalence of anti-HEV IgG increased with age; however, there was no correlation between gender and anti-HEV IgG and/or IgM. The seroprevalence of HEV among blood donors in the Qassim Region was lower than previous estimates for other regions of the country. Further studies covering a wider geographical area are needed to validate and expand the findings and to determine the importance of HEV screening in the region.

scholarly journals Seroprevalence of the hepatitis E virus among blood donors in the Qassim Region, Saudi Arabia

2020 ◽  
Author(s):  
Bader Alhatlani ◽  
Waleed Aljabr ◽  
Mohammed Almarzouqi ◽  
Sami Alhatlani ◽  
Rayan Alzunaydi ◽  
...  
Keyword(s):  
Public Health ◽  
Saudi Arabia ◽  
Blood Transfusion ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Geographical Area ◽  
Hepatitis E ◽  
Public Health Issue ◽  
Serum Samples ◽  
Major Public Health Issue

Abstract ObjectivesTo evaluate the seroprevalence of hepatitis E virus (HEV), a major public health issue worldwide with the potential for transmission via blood transfusion, in blood donors in the Qassim Region, Saudi Arabia. Serum samples (n = 1,078) were collected from volunteer blood donors from January to April 2019 and tested for the presence of anti-HEV IgG and IgM by indirect enzyme-linked immunosorbent assays.ResultsOverall, the seroprevalence of anti-HEV IgG among blood donors was 5.7%. Additionally, the seropositive rates of anti-HEV IgG and IgM were significantly higher in non-Saudi donors (22.1% and 35.3%) than in Saudi donors (3% and 7.4%). The seroprevalence of anti-HEV IgG increased with age; however, there was no correlation between gender and anti-HEV IgG and/or IgM. The seroprevalence of HEV among blood donors in the Qassim Region was lower than previous estimates for other regions of the country. Further studies covering a wider geographical area are needed to validate and expand the findings and to determine the importance of HEV screening in the region.

scholarly journals Seroprevalence of the hepatitis E virus among blood donors in the Qassim Region, Saudi Arabia

2019 ◽  
Author(s):  
Bader Y. Alhatlani ◽  
Waleed A. Aljabr ◽  
Mohammed S. Almarzouqi ◽  
Sami M. Alhatlani ◽  
Rayan N. Alzunaydi ◽  
...  
Keyword(s):  
Public Health ◽  
Saudi Arabia ◽  
Blood Transfusion ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Geographical Area ◽  
Hepatitis E ◽  
Public Health Issue ◽  
Serum Samples ◽  
Major Public Health Issue

AbstractThe aim of this study was to evaluate the seroprevalence of hepatitis E virus (HEV), a major public health issue worldwide with the potential for transmission via blood transfusion, in blood donors in the Qassim Region, Saudi Arabia. Serum samples (n = 1,078) were collected from volunteer blood donors from January to April 2019 and tested for the presence of anti-HEV IgG and IgM by indirect enzyme-linked immunosorbent assays. Overall, the seroprevalence of anti-HEV IgG and IgM among blood donors was 5.7% and 1.3%, respectively. Additionally, the seropositive rates of anti-HEV IgG and IgM were significantly higher in non-Saudi donors (22.1% and 7.8%) than in Saudi donors (3% and 0.2%). The seroprevalence of anti-HEV IgG increased with age; however, there was no correlation between gender and anti-HEV IgG and/or IgM. The seroprevalence of HEV among blood donors in the Qassim Region was lower than previous estimates for other regions of the country. Further studies covering a wider geographical area are needed to validate and expand the findings and to determine the importance of HEV screening in the region.

scholarly journals Low Hepatitis E Virus Prevalence Among Blood Donor in Dali in China

2020 ◽  
Author(s):  
Ping Fu ◽  
Baochai Lin ◽  
Bingting Wu ◽  
Ling Ke ◽  
Tianfu Yang ◽  
...  
Keyword(s):  
Blood Transfusion ◽  
Ethnic Minority ◽  
Odds Ratio ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Hepatitis E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Iga Antibodies ◽  
Positive Rate

Abstract BACKGROUND: Hepatitis E virus (HEV) is a nonenveloped RNA virus causing Hepatitis E worldwide. An increasing transfusion transmission cases of HEV infections from asymptomatic blood donors which causing serious illnesses in immunosuppressed recipients have been reported in the past few years. China is one of the highly prevalent regions of HEV, it is important to evaluate the risk of HEV transmission from blood transfusion. METHODS: A total of 1864 serum samples from blood donors and demographic characteristics were randomly collected from Feb to Mar 2018 in Dali city. Anti-HEV IgG, IgM and IgA antibodies and HEV antigen were examined by enzyme-linked immunosorbent assay (ELISA). HEV RNA was detected by real-time PCR. Multivariable logistic regression modelling was used to examine risk factors associated with HEV prevalence.RESULTS: Overall, the positive rate of anti-HEV IgG, IgM, and IgA antibodies was 13.36% (249/1864), 1.13%(21/1864), and 1.82%(34/1864), respectively. However, none of the 1864 serum samples was detected as HEV antigen-positive nor HEV RNA positive. The positive rate of anti-HEV IgG antibody is high as 28.57% (2/7) in the donors with isolated elevated alanine aminotransferase (ALT). Females (16.69%) had a significantly higher HEV seroprevalence than males (13.04%) (odds ratio [OR]: 1.34 [95% CI, 1.02-1.75]). Other ethnic minority (24.32%) and Bai (18.85%) donors had a significantly higher HEV seroprevalence when compared to Han (12.21%) blood donors (odds ratio [OR], 2.25 [95% CI, 1.04-4.88] for other ethnic minority, 1.65 [95% CI, 1.24-2.19] for Bai). Conclusions: Dali, Yunnan province, China is an endemic region for HEV and have a relatively low risk of HEV transmission via blood transfusion. Whether to formulate the strategy for HEV screening in blood center needed further researched.

scholarly journals Low Hepatitis E virus prevalence among blood donor in Dali in China

2021 ◽  
Author(s):  
Ping Fu ◽  
Baochai Lin ◽  
Bingting Wu ◽  
Ling Ke ◽  
Tianfu Yang ◽  
...  
Keyword(s):  
Risk Factors ◽  
Blood Transfusion ◽  
Ethnic Minority ◽  
Odds Ratio ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Hepatitis E ◽  
Serum Samples ◽  
Iga Antibodies ◽  
Positive Rate

Abstract BACKGROUND: Hepatitis E virus (HEV) is a nonenveloped RNA virus causing Hepatitis E worldwide. An increasing transfusion transmission cases of HEV infections from asymptomatic blood donors which causing serious illnesses in immunosuppressed recipients have been reported in the past few years. China is one of the highly prevalent regions of HEV, it is important to evaluate the risk of HEV transmission from blood transfusion. METHODS: A total of 1864 serum samples from blood donors and demographic characteristics were randomly collected from Feb to Mar 2018 in Dali city. Anti-HEV IgG, IgM and IgA antibodies and HEV antigen were examined by enzyme-linked immunosorbent assay (ELISA). HEV RNA was detected by real-time PCR. Multivariable logistic regression modelling was used to examine risk factors associated with HEV prevalence.RESULTS: Overall, the positive rate of anti-HEV IgG, IgM, and IgA antibodies was 13.36% (249/1864), 1.13%(21/1864), and 1.82%(34/1864), respectively. However, none of the 1864 serum samples was detected as HEV antigen-positive nor HEV RNA positive. The positive rate of anti-HEV IgG antibody is high as 28.57% (2/7) in the donors with isolated elevated alanine aminotransferase (ALT). Females (16.69%) had a significantly higher HEV seroprevalence than males (13.04%) (odds ratio [OR]: 1.34 [95% CI, 1.02-1.75]). Other ethnic minority (24.32%) and Bai (18.85%) donors had a significantly higher HEV seroprevalence when compared to Han (12.21%) blood donors (odds ratio [OR], 2.25 [95% CI, 1.04-4.88] for other ethnic minority, 1.65 [95% CI, 1.24-2.19] for Bai). Conclusions: Dali, Yunnan province, China is an endemic region f of hepatitis E virus, and women, Bai and other ethnic minorities may be potential risk factors for hepatitis E virus infection.. The risk of transmission of hepatitis E virus through blood transfusion is relatively low, and the specific risk value needs to be further tested by expanding the sample size. Whether to formulate the strategy for HEV screening in blood center needed further researched.

scholarly journals Diagnostic Performance of Five Assays for Anti-Hepatitis E Virus IgG and IgM in a Large Cohort Study

2015 ◽  
Vol 54 (3) ◽  
pp. 549-555 ◽  
Author(s):  
Heléne Norder ◽  
Marie Karlsson ◽  
Åsa Mellgren ◽  
Jan Konar ◽  
Elisabeth Sandberg ◽  
...  
Keyword(s):  
Gold Standard ◽  
Diagnostic Performance ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Laboratory Diagnosis ◽  
Hepatitis E ◽  
Serum Samples ◽  
Large Cohort Study ◽  
No Gold Standard

Determination of anti-hepatitis E virus (anti-HEV) antibodies is still enigmatic. There is no gold standard, and results obtained with different assays often diverge. Herein, five assays were compared for detection of anti-HEV IgM and IgG. Serum samples from 500 Swedish blood donors and 316 patients, of whom 136 had suspected HEV infection, were analyzed. Concordant results for IgM and IgG with all assays were obtained only for 71% and 70% of patients with suspected hepatitis E, respectively. The range of sensitivity for anti-HEV detection was broad (42% to 96%); this was reflected in the detection limit, which varied up to 19-fold for IgM and 17-fold for IgG between assays. HEV RNA was analyzed in all patients and in those blood donors reactive for anti-HEV in any assay, and it was found in 26 individuals. Among all of the assays, both anti-HEV IgG and IgM were detected in 10 of those individuals. Twelve had only IgG and, in 7 of those 12, IgG was only detected with the two most sensitive assays. Three of the HEV-RNA-positive samples were negative for anti-HEV IgM and IgG in all assays. With the two most sensitive assays, anti-HEV IgG was identified in 16% of the blood donor samples and in 66% of patients with suspected HEV infection. Because several HEV-RNA-positive samples had only anti-HEV IgG without anti-HEV IgM or lacked anti-HEV antibodies, analysis for HEV RNA may be warranted as a complement in the laboratory diagnosis of ongoing HEV infection.

scholarly journals Seroprevalence of Dromedary Camel HEV in Domestic and Imported Camels from Saudi Arabia

Viruses ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 553
Author(s):  
Sherif Aly El-Kafrawy ◽  
Ahmed Mohamed Hassan ◽  
Mai Mohamed El-Daly ◽  
Ishtiaq Qadri ◽  
Ahmed Majdi Tolah ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Health Concern ◽  
P Value ◽  
Contaminated Water ◽  
Dromedary Camel ◽  
Serum Samples ◽  
Major Health

Hepatitis E Virus (HEV) imposes a major health concern in areas with very poor sanitation in Africa and Asia. The pathogen is transmitted mainly through ingesting contaminated water or food, coming into contact with affected people, and blood transfusions. Very few reports including old reports are available on the prevalence of HEV in Saudi Arabia in humans and no reports exist on HEV prevalence in camels. Dromedary camel trade and farming are increasing in Saudi Arabia with importation occurring unidirectionally from Africa to Saudi Arabia. DcHEV transmission to humans has been reported in one case from the United Arab Emeritus (UAE). This instigated us to perform this investigation of the seroprevalence of HEV in imported and domestic camels in Saudi Arabia. Serum samples were collected from imported and domestic camels. DcHEV-Abs were detected in collected sera using ELISA. The prevalence of DcHEV in the collected samples was 23.1% with slightly lower prevalence in imported camels than domestic camels (22.4% vs. 25.4%, p value = 0.3). Gender was significantly associated with the prevalence of HEV in the collected camels (p value = 0.015) where males (31.6%) were more infected than females (13.4%). This study is the first study to investigate the prevalence of HEV in dromedary camels from Saudi Arabia. The high seroprevalence of DcHEV in dromedaries might indicate their role as a zoonotic reservoir for viral infection to humans. Future HEV seroprevalence studies in humans are needed to investigate the role of DcHEV in the Saudi human population.

Risk of hepatitis‐E virus infections among blood donors in a regional blood transfusion centre in western India

2021 ◽  
Author(s):  
Kanchan K. Mishra ◽  
Krima Patel ◽  
Apeksha Trivedi ◽  
Parizad Patel ◽  
Kanjaksha Ghosh ◽  
...  
Keyword(s):  
Blood Transfusion ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Hepatitis E ◽  
Western India ◽  
Virus Infections

scholarly journals Seroprevalence for hepatitis E virus (HEV) infection among volunteer blood donors of the Regional Blood Bank of Londrina, State of Paraná , Brazil

2006 ◽  
Vol 48 (2) ◽  
pp. 87-92 ◽  
Author(s):  
André Luiz Bortoliero ◽  
Ana Maria Bonametti ◽  
Helena Kaminami Morimoto ◽  
Tiemi Matsuo ◽  
Edna Maria Vissoci Reiche
Keyword(s):  
Urban Areas ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Hepatitis A Virus ◽  
Hepatitis E ◽  
Developed Countries ◽  
Blood Bank ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Sociodemographic Variables

A cross-sectional study was carried out among 996 volunteer blood donors enrolled from May 1999 to December 1999 to determine the seroprevalence of hepatitis E virus (HEV) infection among volunteer blood donors of the Regional Blood Bank of Londrina, State of Paraná, Brazil, and to evaluate whether the rate of seroprevalence of IgG anti-HEV antibodies is associated with sociodemographic variables and with seropositivity for hepatitis A virus (HAV) infection. All participants answered the questionnaire regarding the sociodemographic characterisitcs. Serum samples were tested for IgG antibodies to HEV (anti-HEV) by an enzyme linked immunoassay (ELISA). All serum samples positive for anti-HEV IgG and 237 serum samples negative for anti-HEV were also assayed for IgG anti-HAV antibodies by ELISA. Anti-HEV IgG was confirmed in 23/996 samples, resulting in a seroprevalence of 2.3% for HEV infection, similar to previous results obtained in developed countries. No significant association was found between the presence of anti-HEV IgG antibodies and the sociodemographic variables including gender, age, educational level, rural or urban areas, source of water, and sewer system (p > 0.05). Also, no association with seropositivity for anti-HAV IgG antibodies was observed (p > 0.05). Although this study revealed a low seroprevalence of HEV infection in the population evaluated, the results showed that this virus is circulating among the population from Londrina, South Brazil, and point out the need of further studies to define the clinical and epidemiological importance of HEV infection and to identify additional risk factors involved in the epidemiology and pathogenesis of this infection in this population.

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