Low Hepatitis E virus prevalence among blood donor in Dali in China

Abstract BACKGROUND: Hepatitis E virus (HEV) is a nonenveloped RNA virus causing Hepatitis E worldwide. An increasing transfusion transmission cases of HEV infections from asymptomatic blood donors which causing serious illnesses in immunosuppressed recipients have been reported in the past few years. China is one of the highly prevalent regions of HEV, it is important to evaluate the risk of HEV transmission from blood transfusion. METHODS: A total of 1864 serum samples from blood donors and demographic characteristics were randomly collected from Feb to Mar 2018 in Dali city. Anti-HEV IgG, IgM and IgA antibodies and HEV antigen were examined by enzyme-linked immunosorbent assay (ELISA). HEV RNA was detected by real-time PCR. Multivariable logistic regression modelling was used to examine risk factors associated with HEV prevalence.RESULTS: Overall, the positive rate of anti-HEV IgG, IgM, and IgA antibodies was 13.36% (249/1864), 1.13%(21/1864), and 1.82%(34/1864), respectively. However, none of the 1864 serum samples was detected as HEV antigen-positive nor HEV RNA positive. The positive rate of anti-HEV IgG antibody is high as 28.57% (2/7) in the donors with isolated elevated alanine aminotransferase (ALT). Females (16.69%) had a significantly higher HEV seroprevalence than males (13.04%) (odds ratio [OR]: 1.34 [95% CI, 1.02-1.75]). Other ethnic minority (24.32%) and Bai (18.85%) donors had a significantly higher HEV seroprevalence when compared to Han (12.21%) blood donors (odds ratio [OR], 2.25 [95% CI, 1.04-4.88] for other ethnic minority, 1.65 [95% CI, 1.24-2.19] for Bai). Conclusions: Dali, Yunnan province, China is an endemic region f of hepatitis E virus, and women, Bai and other ethnic minorities may be potential risk factors for hepatitis E virus infection.. The risk of transmission of hepatitis E virus through blood transfusion is relatively low, and the specific risk value needs to be further tested by expanding the sample size. Whether to formulate the strategy for HEV screening in blood center needed further researched.

Download Full-text

Low Hepatitis E Virus Prevalence Among Blood Donor in Dali in China

10.21203/rs.3.rs-118265/v1 ◽

2020 ◽

Author(s):

Ping Fu ◽

Baochai Lin ◽

Bingting Wu ◽

Ling Ke ◽

Tianfu Yang ◽

...

Keyword(s):

Blood Transfusion ◽

Ethnic Minority ◽

Odds Ratio ◽

Hepatitis E Virus ◽

Blood Donors ◽

Hepatitis E ◽

Enzyme Linked Immunosorbent Assay ◽

Serum Samples ◽

Iga Antibodies ◽

Positive Rate

Abstract BACKGROUND: Hepatitis E virus (HEV) is a nonenveloped RNA virus causing Hepatitis E worldwide. An increasing transfusion transmission cases of HEV infections from asymptomatic blood donors which causing serious illnesses in immunosuppressed recipients have been reported in the past few years. China is one of the highly prevalent regions of HEV, it is important to evaluate the risk of HEV transmission from blood transfusion. METHODS: A total of 1864 serum samples from blood donors and demographic characteristics were randomly collected from Feb to Mar 2018 in Dali city. Anti-HEV IgG, IgM and IgA antibodies and HEV antigen were examined by enzyme-linked immunosorbent assay (ELISA). HEV RNA was detected by real-time PCR. Multivariable logistic regression modelling was used to examine risk factors associated with HEV prevalence.RESULTS: Overall, the positive rate of anti-HEV IgG, IgM, and IgA antibodies was 13.36% (249/1864), 1.13%(21/1864), and 1.82%(34/1864), respectively. However, none of the 1864 serum samples was detected as HEV antigen-positive nor HEV RNA positive. The positive rate of anti-HEV IgG antibody is high as 28.57% (2/7) in the donors with isolated elevated alanine aminotransferase (ALT). Females (16.69%) had a significantly higher HEV seroprevalence than males (13.04%) (odds ratio [OR]: 1.34 [95% CI, 1.02-1.75]). Other ethnic minority (24.32%) and Bai (18.85%) donors had a significantly higher HEV seroprevalence when compared to Han (12.21%) blood donors (odds ratio [OR], 2.25 [95% CI, 1.04-4.88] for other ethnic minority, 1.65 [95% CI, 1.24-2.19] for Bai). Conclusions: Dali, Yunnan province, China is an endemic region for HEV and have a relatively low risk of HEV transmission via blood transfusion. Whether to formulate the strategy for HEV screening in blood center needed further researched.

Download Full-text

Hepatitis E virus prevalence among blood donors in Dali, China

Virology Journal ◽

10.1186/s12985-021-01607-y ◽

2021 ◽

Vol 18 (1) ◽

Author(s):

Ping Fu ◽

Baochai Lin ◽

Bingting Wu ◽

Ling Ke ◽

Tianfu Yang ◽

...

Keyword(s):

Odds Ratio ◽

Hepatitis E Virus ◽

Blood Donors ◽

Rna Virus ◽

Hepatitis E ◽

Enzyme Linked Immunosorbent Assay ◽

Serum Samples ◽

The Past ◽

Virus Prevalence ◽

Iga Antibodies

Abstract Background Hepatitis E virus (HEV) is a nonenveloped RNA virus causing hepatitis E worldwide. The increase in transfusion-transmitted cases of HEV infections from asymptomatic blood donors causing serious illnesses among immunosuppressed recipients has been reported in the past few years. China is one of the most prevalent regions of HEV; as a result, it is important to evaluate the risk of transfusion-transmitted HEV. Methods A total of 1864 serum samples (including demographic characteristics) from blood donors were randomly collected from February to March 2018 in Dali city. Anti-HEV IgG, IgM and IgA antibodies and HEV antigen were examined by enzyme-linked immunosorbent assay (ELISA). HEV RNA was detected by real-time PCR. Multivariable logistic regression modelling was used to examine the risk factors associated with HEV prevalence. Results Overall, the positive rates of anti-HEV IgG, IgM, and IgA antibodies were 13.36% (249/1864), 1.13% (21/1864), and 1.82% (34/1864), respectively. However, none of the 1864 serum samples were HEV antigen positive or HEV RNA positive. Females (16.69%) had a significantly higher HEV seroprevalence than males (13.04%) (odds ratio [OR] 1.34 [95% CI, 1.02–1.75]). Bai (18.85%) donors had a significantly higher HEV seroprevalence than Han (12.21%) blood donors (odds ratio [OR], 1.65 [95% CI, 1.24–2.19] for Bai). Conclusions HEV showed a seroprevalence among blood donors in Yunnan Province, some of which were even recent infections, indicating a threat to the safety of blood transfusions. Whether to formulate a strategy for HEV screening in blood centres needs further research.

Download Full-text

Seroprevalence of the hepatitis E virus antibodies among blood donors in the Qassim region, Saudi Arabia

Future Virology ◽

10.2217/fvl-2021-0013 ◽

2021 ◽

Author(s):

Bader Y Alhatlani ◽

Waleed A Aljabr ◽

Mohammed S Almarzouqi ◽

Sami M Alhatlani ◽

Rayan N Alzunaydi ◽

...

Keyword(s):

Public Health ◽

Saudi Arabia ◽

Blood Transfusion ◽

Hepatitis E Virus ◽

Blood Donors ◽

Indirect Elisa ◽

Hepatitis E ◽

Public Health Issue ◽

Serum Samples ◽

Major Public Health Issue

Aim: Hepatitis E virus (HEV) transmission through blood transfusion is a major public health issue worldwide. We aimed to determine the seroprevalence of HEV in blood donors in the Qassim region of Saudi Arabia. Materials & methods: Serum samples (n = 1078) were collected from volunteer blood donors and tested for the presence of anti-HEV IgG and IgM by indirect ELISA. Results: The seroprevalence of anti-HEV IgG among the blood donors was 5.7% overall. Anti-HEV IgG and IgM seropositivity were significantly higher in non-Saudi donors than in Saudi donors (22.1 vs 3 and 7.8 vs 0.2% for anti-HEV IgG and IgM, respectively). Conclusion: The seroprevalence of HEV among blood donors in the Qassim region was lower than previous estimates for other regions of the country and neighboring countries.

Download Full-text

Seroprevalence of the hepatitis E virus among blood donors in the Qassim Region, Saudi Arabia

10.21203/rs.3.rs-18073/v1 ◽

2020 ◽

Author(s):

Bader Alhatlani ◽

Waleed Aljabr ◽

Mohammed Almarzouqi ◽

Sami Alhatlani ◽

Rayan Alzunaydi ◽

...

Keyword(s):

Public Health ◽

Saudi Arabia ◽

Blood Transfusion ◽

Hepatitis E Virus ◽

Blood Donors ◽

Geographical Area ◽

Hepatitis E ◽

Public Health Issue ◽

Serum Samples ◽

Major Public Health Issue

Abstract Objectives The aim of this study was to evaluate the seroprevalence of hepatitis E virus (HEV), a major public health issue worldwide with the potential for transmission via blood transfusion, in blood donors in the Qassim Region, Saudi Arabia. Serum samples (n = 1,078) were collected from volunteer blood donors from January to April 2019 and tested for the presence of anti-HEV IgG and IgM by indirect enzyme-linked immunosorbent assays. Results Overall, the seroprevalence of anti-HEV IgG and IgM among blood donors was 5.7% and 1.3%, respectively. Additionally, the seropositive rates of anti-HEV IgG and IgM were significantly higher in non-Saudi donors (22.1% and 7.8%) than in Saudi donors (3% and 0.2%). The seroprevalence of anti-HEV IgG increased with age; however, there was no correlation between gender and anti-HEV IgG and/or IgM. The seroprevalence of HEV among blood donors in the Qassim Region was lower than previous estimates for other regions of the country. Further studies covering a wider geographical area are needed to validate and expand the findings and to determine the importance of HEV screening in the region.

Download Full-text

Seroprevalence of the hepatitis E virus among blood donors in the Qassim Region, Saudi Arabia

10.21203/rs.3.rs-18073/v2 ◽

2020 ◽

Author(s):

Bader Alhatlani ◽

Waleed Aljabr ◽

Mohammed Almarzouqi ◽

Sami Alhatlani ◽

Rayan Alzunaydi ◽

...

Keyword(s):

Public Health ◽

Saudi Arabia ◽

Blood Transfusion ◽

Hepatitis E Virus ◽

Blood Donors ◽

Geographical Area ◽

Hepatitis E ◽

Public Health Issue ◽

Serum Samples ◽

Major Public Health Issue

Abstract ObjectivesTo evaluate the seroprevalence of hepatitis E virus (HEV), a major public health issue worldwide with the potential for transmission via blood transfusion, in blood donors in the Qassim Region, Saudi Arabia. Serum samples (n = 1,078) were collected from volunteer blood donors from January to April 2019 and tested for the presence of anti-HEV IgG and IgM by indirect enzyme-linked immunosorbent assays.ResultsOverall, the seroprevalence of anti-HEV IgG among blood donors was 5.7%. Additionally, the seropositive rates of anti-HEV IgG and IgM were significantly higher in non-Saudi donors (22.1% and 35.3%) than in Saudi donors (3% and 7.4%). The seroprevalence of anti-HEV IgG increased with age; however, there was no correlation between gender and anti-HEV IgG and/or IgM. The seroprevalence of HEV among blood donors in the Qassim Region was lower than previous estimates for other regions of the country. Further studies covering a wider geographical area are needed to validate and expand the findings and to determine the importance of HEV screening in the region.

Download Full-text

Seroprevalence of the hepatitis E virus among blood donors in the Qassim Region, Saudi Arabia

10.1101/2019.12.19.19015412 ◽

2019 ◽

Author(s):

Bader Y. Alhatlani ◽

Waleed A. Aljabr ◽

Mohammed S. Almarzouqi ◽

Sami M. Alhatlani ◽

Rayan N. Alzunaydi ◽

...

Keyword(s):

Public Health ◽

Saudi Arabia ◽

Blood Transfusion ◽

Hepatitis E Virus ◽

Blood Donors ◽

Geographical Area ◽

Hepatitis E ◽

Public Health Issue ◽

Serum Samples ◽

Major Public Health Issue

AbstractThe aim of this study was to evaluate the seroprevalence of hepatitis E virus (HEV), a major public health issue worldwide with the potential for transmission via blood transfusion, in blood donors in the Qassim Region, Saudi Arabia. Serum samples (n = 1,078) were collected from volunteer blood donors from January to April 2019 and tested for the presence of anti-HEV IgG and IgM by indirect enzyme-linked immunosorbent assays. Overall, the seroprevalence of anti-HEV IgG and IgM among blood donors was 5.7% and 1.3%, respectively. Additionally, the seropositive rates of anti-HEV IgG and IgM were significantly higher in non-Saudi donors (22.1% and 7.8%) than in Saudi donors (3% and 0.2%). The seroprevalence of anti-HEV IgG increased with age; however, there was no correlation between gender and anti-HEV IgG and/or IgM. The seroprevalence of HEV among blood donors in the Qassim Region was lower than previous estimates for other regions of the country. Further studies covering a wider geographical area are needed to validate and expand the findings and to determine the importance of HEV screening in the region.

Download Full-text

Diagnostic Performance of Five Assays for Anti-Hepatitis E Virus IgG and IgM in a Large Cohort Study

Journal of Clinical Microbiology ◽

10.1128/jcm.02343-15 ◽

2015 ◽

Vol 54 (3) ◽

pp. 549-555 ◽

Cited By ~ 55

Author(s):

Heléne Norder ◽

Marie Karlsson ◽

Åsa Mellgren ◽

Jan Konar ◽

Elisabeth Sandberg ◽

...

Keyword(s):

Gold Standard ◽

Diagnostic Performance ◽

Hepatitis E Virus ◽

Blood Donors ◽

Laboratory Diagnosis ◽

Hepatitis E ◽

Serum Samples ◽

Large Cohort Study ◽

No Gold Standard

Determination of anti-hepatitis E virus (anti-HEV) antibodies is still enigmatic. There is no gold standard, and results obtained with different assays often diverge. Herein, five assays were compared for detection of anti-HEV IgM and IgG. Serum samples from 500 Swedish blood donors and 316 patients, of whom 136 had suspected HEV infection, were analyzed. Concordant results for IgM and IgG with all assays were obtained only for 71% and 70% of patients with suspected hepatitis E, respectively. The range of sensitivity for anti-HEV detection was broad (42% to 96%); this was reflected in the detection limit, which varied up to 19-fold for IgM and 17-fold for IgG between assays. HEV RNA was analyzed in all patients and in those blood donors reactive for anti-HEV in any assay, and it was found in 26 individuals. Among all of the assays, both anti-HEV IgG and IgM were detected in 10 of those individuals. Twelve had only IgG and, in 7 of those 12, IgG was only detected with the two most sensitive assays. Three of the HEV-RNA-positive samples were negative for anti-HEV IgM and IgG in all assays. With the two most sensitive assays, anti-HEV IgG was identified in 16% of the blood donor samples and in 66% of patients with suspected HEV infection. Because several HEV-RNA-positive samples had only anti-HEV IgG without anti-HEV IgM or lacked anti-HEV antibodies, analysis for HEV RNA may be warranted as a complement in the laboratory diagnosis of ongoing HEV infection.

Download Full-text

Case–Control Study of Risk Factors for Acquired Hepatitis E Virus Infections in Blood Donors, United Kingdom, 2018–2019

Emerging Infectious Diseases ◽

10.3201/eid2706.203964 ◽

2021 ◽

Vol 27 (6) ◽

pp. 1654-1661

Author(s):

Iona Smith ◽

Bengü Said ◽

Aisling Vaughan ◽

Becky Haywood ◽

Samreen Ijaz ◽

...

Keyword(s):

Risk Factors ◽

United Kingdom ◽

Hepatitis E Virus ◽

Blood Donors ◽

Case Control Study ◽

Hepatitis E ◽

Case Control ◽

Virus Infections ◽

Control Study

Download Full-text

Seroprevalence and Risk Factors Associated with Hepatitis E Virus Infection in Three Species of Pet Birds in Northwest China

The Scientific World JOURNAL ◽

10.1155/2014/296285 ◽

2014 ◽

Vol 2014 ◽

pp. 1-4 ◽

Cited By ~ 3

Author(s):

Wei Cong ◽

Qing-Feng Meng ◽

Xiao-Feng Shan ◽

Wu-Wen Sun ◽

Si-Yuan Qin ◽

...

Keyword(s):

Risk Factors ◽

Virus Infection ◽

Enzyme Immunoassay ◽

Causative Agent ◽

Hepatitis E Virus ◽

Hepatitis E ◽

Northwest China ◽

Serum Samples ◽

Factors Associated ◽

Pet Birds

Hepatitis E virus (HEV), the causative agent of hepatitis E, has been reported in a wide variety of animals, including birds, but little is known of HEV infection in pet birds in northwest China. The objective of the present investigation was to examine HEV seroprevalence in three species of pet birds, namely, Eurasian siskin, Oriental skylark, and black-tailed grosbeak from Gansu. Serum samples collected from 685 pet birds from August 2011 to September 2012 were examined independently for the presence of antibodies against HEV. A total of 59 (8.31%) pet birds were tested positive for HEV antibodies by the commercially available enzyme immunoassay kits. Of these, the seroprevalence was diverse in different species pet birds; the most frequent level was 10.83% (39/360) in Eurasian siskin, followed by 6.57% (19/289) in Oriental skylark, and 2.29% (1/36) in black-tailed grosbeak. Age and collecting region of pet birds were the main risk factors associated with HEV infection. The present study firstly revealed the seroprevalence of HEV infection in three species of pet birds in northwest China, which provided the baseline data for taking comprehensive countermeasures and measures for effectively preventing and controlling HEV infection in birds.

Download Full-text