EFECTO DE LA ZEOLITA NATURAL CLINOPTILITA SOBRE EL CRECIMIENTO DE DUNALIELLA SALINA (TEODORESCO, 1905) CULTIVADA EN UN FOTOBIORREACTOR DE MÚLTIPLES CÁMARAS OSCILANTES

2013 ◽  
Vol 6 ◽  
Author(s):  
Euler Gallego Cartagena ◽  
Leydis Yohana Herrera Britto ◽  
Lena Judith Manjarrez Rodríguez ◽  
Carmiña Lucía Vargas Zapata
Keyword(s):  
Cell Density ◽  
Dunaliella Salina ◽  
Logarithmic Phase ◽  
Total Chlorophyll ◽  
Maximum Cell Density ◽  
Stages Of Growth ◽  
Maximum Cell ◽  
Level Of Significance ◽  
Duplication Time ◽  
Chloroplastic Pigments

We studied the growth and production of pigment of microalgae Dunaliella salina cultivated in<br />photobioreactor multicamera oscillating outside laboratory conditions to evaluate the effect of different<br />concentrations of zeolite (ZC). Growth was evaluated by cell count and pigment content was performed<br />by spectrophotometric techniques. The results indicate that the concentration of 50 mgL-1 of ZC produced<br />a better stimulus on the growth of the microalga reaching maximum cell density (MDC) of 5.51 ±0.45 x 106<br />celmL-1, growth rate (μ) 0.37 ±0.03 divday-1 and duplication time (Td) of 1.87 ±0.02 days. Likewise,<br />produced a greater increase in the total chlorophyll and carotenoids in the logarithmic phase of values<br />15.554 ±0.77 and 0.50 ±0.01 mgmL-1, respectively. Chloroplastic pigments concentration per volume of<br />culture has a significant correlation with maximum cell density of D. salina treatments based on zeolite at<br />all stages of growth with rMDC, chla.tot= 0.89 y una rMDC, carot.tot=0.926 at a level of significance (p &lt;0.01). The<br />results demonstrated the feasibility of using this product as a suitable substrate for the growth of the<br />microalga, being an innovative alternative and less costly to obtain significant metabolites.

A Comparison of CO2 and N2 Foaming Behaviors of PP in a Visualization System

2020 ◽  
Vol 35 (5) ◽  
pp. 503-517
Author(s):  
Q.-P. Guo ◽  
J. Wang ◽  
C. B. Park
Keyword(s):  
Cell Density ◽  
Nucleating Agent ◽  
Gas Content ◽  
Experimental Conditions ◽  
Maximum Cell Density ◽  
Visualization System ◽  
Cell Nucleation ◽  
Processing Strategies ◽  
Automotive Parts ◽  
Maximum Cell

Abstract Understanding of polypropylene (PP) foaming is critically important to reduce the weight of automotive parts. In this study, we used a batch foaming simulation system with visualization cell, to observe the foaming behaviors of PP that is blown with CO2 and N2 under various experimental conditions. We found that the nucleating agent content, initial temperature, pressure (i. e., gas content), and pressure drop rate during foaming have a significant effect on cell nucleation and cell growth. The cell density and the void fraction of PP foamed with CO2 and N2, respectively, were separately observed and compared. It was found that under the same experimental conditions, the maximum cell density of PP foamed with CO2 was higher than that of PP foamed with N2. However, the maximum cell density of PP foamed with CO2 was determined to be lower than that of PP foamed with N2, when the same gas mole numbers were employed. Based on the experimental results, optimum foaming conditions and effective processing strategies for PP-CO2 system are suggested.

scholarly journals Comparison of industrial-scale tubular photobioreactor to FRP (fiberglass reinforced plastic) panel photobioreactor on outdoor culture of Nannochloropsis oculata in the marine hatchery

2020 ◽  
Vol 37 (3) ◽  
pp. 303-308
Author(s):  
Yasar Durmaz ◽  
Gökhun Çagatay Erbil
Keyword(s):  
Cell Density ◽  
Marine Fish ◽  
Nannochloropsis Oculata ◽  
Maximum Cell Density ◽  
Tubular Photobioreactor ◽  
Live Feed ◽  
Microalgal Culture ◽  
Reinforced Plastic ◽  
Plastic Panel ◽  
Maximum Cell

Microalgal culture is a key procedure in marine fish hatcheries, but this activity is far from optimized and has several problems remain to be solved. Nannochloropsis oculata are important to live feed organisms, which are used to rear the larvae of marine finfish. N. oculata were cultivated in tubular PBR and FRP panel PBR in a greenhouse. Tubular PBR was reached 701.7 x 106 cells mL-1 as its maximum cell density and FRP panel PBR was reached 245 x 106 cells mL-1 as maximum. Also, estimated maximum dry weights of tubular and FRP panel PBRs were calculated as 3.249 g L-1 and 1.47 g L-1, respectively. Consequently, tubular PBR was showed that it is more efficient than FRP panel PBR in this study.

scholarly journals ViableTM AC-2, a New Adult Bovine Serum- and Colostrum-Based Supplement for the Culture of Mammalian Cells

BioTechniques ◽  
1996 ◽  
Vol 20 (4) ◽  
pp. 702-707
Author(s):  
Britta Viander ◽  
Sari Ala-Uotila ◽  
Markku Jalkanen ◽  
Raimo Pakkanen
Keyword(s):  
Cell Line ◽  
Cell Density ◽  
Cell Lines ◽  
Bovine Serum ◽  
Receptor Expression ◽  
Small Scale ◽  
Maximum Cell Density ◽  
Mammary Tumor Cell ◽  
Adult Bovine Serum ◽  
Maximum Cell

In this study we have shown that ViableTM AC-2, a medium based on an ultrafiltrate fraction of bovine colostrum and adult bovine serum, can be used successfully as a fetal bovine serum (FBS) substitute in the culture of several anchorage-dependent and independent cell lines. Of the 15 cell lines cultured in 8% Viable AC-2 in microplates, 10 reached the maximum cell density of 65%-123% of that in 10% FBS, 4 cell lines reached maximum cell density of 35%-65% of that in 10% FBS and only one cell line, a human osteosawoma G-292, grew slowly in Viable AC-2. In a small-scale suspension culture, 8%-15% Viable AC-2 supports the growth of Chinese hamster ovary cells (CHO-K1) on microcarriers in spinner flasks significantly better than 10% FBS. Shionogi mouse mammary tumor cell line (S115) transfected with human α2-adrenergic receptor subtype C2 was used as a model to study recombinant protein production in Viable AC-2-supplemented medium. The results showed that in cell culture flasks and in an ACUSYST-RTM bioreactot, the α2-C2 receptor expression level per mg of total protein was similar in both Viable AC-2 and FBS.

scholarly journals Strategies for the expansion of human induced pluripotent stem cells as aggregates in single-use Vertical-Wheel™ bioreactors

2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Diogo E. S. Nogueira ◽  
Carlos A. V. Rodrigues ◽  
Marta S. Carvalho ◽  
Cláudia C. Miranda ◽  
Yas Hashimura ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Cell Density ◽  
Pluripotent Stem Cells ◽  
Dextran Sulfate ◽  
Maximum Cell Density ◽  
Differentiation Potential ◽  
Single Use ◽  
Maximum Cell ◽  
Induced Pluripotent

Abstract Background Since their inception, human induced pluripotent stem cells (hiPSCs) have held much promise for pharmacological applications and cell-based therapies. However, their potential can only be realised if large numbers of cells can be produced reproducibly on-demand. While bioreactors are ideal systems for this task, due to providing agitation and control of the culture parameters, the common impeller geometries were not designed for the expansion of mammalian cells, potentially leading to sub-optimal results. Results This work reports for the first time the usage of the novel Vertical-Wheel single-use bioreactors for the expansion of hiPSCs as floating aggregates. Cultures were performed in the PBS MINI 0.1 bioreactor with 60 mL of working volume. Two different culture media were tested, mTeSR1 and mTeSR3D, in a repeated batch or fed-batch mode, respectively, as well as dextran sulfate (DS) supplementation. mTeSR3D was shown to sustain hiPSC expansion, although with lower maximum cell density than mTeSR1. Dextran sulfate supplementation led to an increase in 97 and 106% in maximum cell number when using mTeSR1 or mTeSR3D, respectively. For supplemented media, mTeSR1 + DS allowed for a higher cell density to be obtained with one less day of culture. A maximum cell density of (2.3 ± 0.2) × 106 cells∙mL− 1 and a volumetric productivity of (4.6 ± 0.3) × 105 cells∙mL− 1∙d− 1 were obtained after 5 days with mTeSR1 + DS, resulting in aggregates with an average diameter of 346 ± 11 μm. The generated hiPSCs were analysed by flow cytometry and qRT-PCR and their differentiation potential was assayed, revealing the maintenance of their pluripotency after expansion. Conclusions The results here described present the Vertical-Wheel bioreactor as a promising technology for hiPSC bioprocessing. The specific characteristics of this bioreactor, namely in terms of the innovative agitation mechanism, can make it an important system in the development of hiPSC-derived products under current Good Manufacturing Practices.

Listeria monocytogenes Serotype 1/2b and 4b Isolates from Human Clinical Cases and Foods Show Differences in Tolerance to Refrigeration and Salt Stress

2014 ◽  
Vol 77 (9) ◽  
pp. 1519-1526 ◽  
Author(s):  
V. B. RIBEIRO ◽  
M. T. DESTRO
Keyword(s):  
Listeria Monocytogenes ◽  
Cell Density ◽  
Brain Heart Infusion ◽  
Lag Phase ◽  
Maximum Cell Density ◽  
Significant Difference ◽  
Serotype 1 ◽  
Maximum Cell ◽  
Serotype 4B ◽  
Clinical Cases

Control of Listeria monocytogenes in food processing facilities is a difficult issue because of the ability of this microorganism to form biofilms and adapt to adverse environmental conditions. Survival at high concentrations of sodium chloride and growth at refrigeration temperatures are two other important characteristics of L. monocytogenes isolates. The aim of this study was to compare the growth characteristics under stress conditions at different temperatures of L. monocytogenes serotypes responsible for the majority of clinical cases from different sources. Twenty-two L. monocytogenes isolates, 12 from clinical cases (8 serotype 4b and 4 serotype 1/2a) and 10 from food (6 serotype 4b and 4 serotype 1/2a), and an L. monocytogenes Scott A (serotype 4b) reference strain were analyzed for the ability to grow in brain heart infusion broth plus 1.9 M NaCl (11%) at 4, 10, and 25°C for 73, 42, and 15 days, respectively. The majority of L. monocytogenes strains was viable or even grew at 4°C and under the high osmotic conditions usually used to control pathogens in the food industry. At 10°C, most strains could adapt and grow; however, no significant difference (P &gt; 0.05) was found for lag-phase duration, maximum growth rate, and maximum cell density. At 25°C, all strains were able to grow, and populations increased by up 5 log CFU/ml. Clinical strains had a significantly longer lag phase and lower maximum cell density (P &lt; 0.05) than did food strains. Regarding virulence potential, no significant differences in hemolytic activity were found among serotypes; however, serotype 4b strains were more invasive in Caco-2 cells than were serotype 1/2a strains (P &lt; 0.05). The global tendency of decreasing NaCl concentrations in processed foods for health reasons may facilitate L. monocytogenes survival and growth in these products. Therefore, food companies must consider additional microbial growth barriers to assure product safety.

The Effects of Ninjinyoeito on Human Vascular Endothelial Cells

1993 ◽  
Vol 21 (03n04) ◽  
pp. 279-289 ◽  
Author(s):  
Yasuhiko Uchiyama ◽  
Shigekatsu Nakajima ◽  
Kazuo Yoshida ◽  
Hiromi Mizukawa ◽  
Eiichi Haruki
Keyword(s):  
Endothelial Cells ◽  
Cell Density ◽  
Vascular Endothelial Cells ◽  
Umbilical Vein ◽  
Aging Effect ◽  
Inhibitory Effect ◽  
Vascular Endothelial ◽  
Maximum Cell Density ◽  
Human Umbilical Vein ◽  
Maximum Cell

The effects of Ninjinyoeito on endothelial cells from a human umbilical vein were examined. As a result, the maximum cell density obtained after the addition of Ninjinyoeito was found to be 400 ng/ml, and its inhibitory effect on the development of endothelial injury was also observed. In addition, the levels of LDH and Al-p, which are parameters decreasing with aging, were significantly high. Judging from these results, Ninjinyoeito presumably exerts an anti-aging effect by inhibiting the metabolic aging of cells.

scholarly journals Evaluation of growth of Chlorella ellipsoidea in different culture media

2015 ◽  
Vol 4 (2) ◽  
pp. 6-10
Author(s):  
F Jahan ◽  
MS Rahman ◽  
MA Hossain
Keyword(s):  
Cell Density ◽  
Culture Media ◽  
Soil Extract ◽  
Total Alkalinity ◽  
Algal Culture ◽  
Chlorella Ellipsoidea ◽  
Maximum Cell Density ◽  
Inorganic Medium ◽  
Maximum Cell ◽  
Cell Densities

An experiment was conducted to evaluate the growth of Chlorella ellipsoidea in three different media viz,. medium I (pulse bran), medium II (soil extract) and medium III (inorganic) under the natural environmental conditions. The alga, C. ellipsoidea, reached maximum cell density of 56.32 × 106 cells ml-1 in 10 days in medium I (pulse bran), maximum cell density of 102.99 × 106 cells ml-1 in 11 days in medium II (soil extract) and maximum cell density of 64.23 × 106 cells ml-1 in 12 days in medium III (inorganic medium). The ranges of water temperature, air temperature and light intensity were 22 to 32ºC, 22 to 34ºC and 2.11 to 4.31 (× 103) lux, respectively during the culture period. The average sunshine period was 7.65 ± 1.57 hours. Total alkalinity, free CO2, pH, NO3-N, PO4- P of algal culture medium I, medium II and medium III were 220, 200 and 150 mg L-1 ; 26, 9 and 19 mg L-1; 7.9, 7.6 and 7.5; 45, 45 and 133.33 mg L-1; 10.9, 15.1 and 37.06 mg L-1, respectively. Cell densities of cultures of C. ellipsoidea under three treatments I, II and III, it can be concluded that cell densities under 3 treatments are significantly different (F=39.78) and treatment II (soil extract medium) is the best for algal (C. ellipsoidea) culture among three treatments. DOI: http://dx.doi.org/10.3329/ijarit.v4i2.22636 Int. J. Agril. Res. Innov. & Tech. 4 (2): 6-10, December, 2014

scholarly journals A novel scale-up strategy for cultivation of BHK-21 cells based on similar hydrodynamic environments in the bioreactors

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Xiaonuo Teng ◽  
Chao Li ◽  
Xiaoping Yi ◽  
Yingping Zhuang
Keyword(s):  
Cell Density ◽  
Animal Cell ◽  
Scale Up ◽  
Virus Titer ◽  
Cell Aggregation ◽  
Agitation Speed ◽  
Cell Cultivation ◽  
Maximum Cell Density ◽  
Aggregation Rate ◽  
Maximum Cell

AbstractThe scale-up of animal cell cultivation is important but remains complex and challenging. In the present study, we propose a novel scale-up strategy for baby hamster Syrian kidney-21 (BHK-21) cell cultivation based on similar hydrodynamic environments. The hydrodynamic characteristics of the different scale bioreactors were determined by computational fluid dynamics (CFD) and further correlated with the agitation speed. The optimal hydrodynamic environment for cell cultivation and vaccine production was determined from the cultivation experiments of BHK-21 cells in 5-L laboratory-scale bioreactors equipped with different impellers at various agitation speeds. BHK-21 cell cultivation was scaled up from 5-L to 42-, 350-, and 1000-L bioreactors by adjusting the agitation speed to make the hydrodynamic features similar to those in the 5-L bioreactor, especially for the shear rate in the impeller zone (γimp) and energy dissipation rate in the tank bulk zone (εtan). The maximum cell density and cell aggregation rate in these scaled-up bioreactors were in the range of 4.6 × 106 ~ 4.8 × 106 cells/mL and 16 ~ 20%, which are comparable to or even better than those observed in the 5-L bioreactor (maximum cell density 4.8 × 106 cells/mL, cell aggregation rate 21%). The maximum virus titer of 108.0 LD50/mL achieved in the 1000-L bioreactor was close to 108.3 LD50/mL that obtained in the 5-L bioreactor. Hence, the scale-up strategy proposed in this study is feasible and can efficiently facilitate the scale-up processes of animal cell cultivation.

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