scholarly journals Effects of Cultural Conditions in Enhancing the Production of Anti-MRSA Activity of Lasiodiplodia pseudotheobromae IBRL OS-64, an Endophytic Fungus Isolated from Leaf of Ocimum sanctum L. in Submerged Fermentation System

2019 ◽  
Vol 13 (4) ◽  
pp. 2517-2531
Author(s):  
M.M.J. Taufiq ◽  
I. Darah
Keyword(s):  
Endophytic Fungus ◽  
Submerged Fermentation ◽  
Ocimum Sanctum ◽  
Fermentation System ◽  
Cultural Conditions

scholarly journals Development of a New Bioprocess for Clean Diosgenin Production through Submerged Fermentation of an Endophytic Fungus

ACS Omega ◽  
2021 ◽  
Author(s):  
Wancang Liu ◽  
Haibo Xiang ◽  
Tao Zhang ◽  
Xu Pang ◽  
Jing Su ◽  
...  
Keyword(s):  
Endophytic Fungus ◽  
Submerged Fermentation

Optimization of cultural conditions affecting improved bioactive metabolite production by endophytic fungus Trichoderma harzianum

Bionatura ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 2187-2192
Author(s):  
Rashid Rahim Hateet ◽  
Zainab Alag Hassan ◽  
Abdulameer Abdullah Al-Mussawi ◽  
Shaima Rabeea Banoon
Keyword(s):  
Antibacterial Activity ◽  
Trichoderma Harzianum ◽  
Endophytic Fungus ◽  
Nitrogen Sources ◽  
Bioactive Metabolites ◽  
Bioactive Metabolite ◽  
Metabolite Production ◽  
Surface Sterilization ◽  
Cultural Conditions

The present study aimed to optimize cultural conditions for optimum bioactive metabolite production by endophytic fungus Trichoderma harzianum, isolated by surface sterilization method from the leaf of the eucalyptus plant. The fungus was identified based on morphological characterization. Fungal metabolites were carried out by ethyl acetate solvent. The antibacterial activity was tested against Escherichia coli (ATCC 25922) and Staphylococcus aureus (NCTC 6571). Various carbon, nitrogen sources, pH, temperature, incubation period, and NaCl on the antibacterial metabolite production were studied. Bioactive metabolite production of T. harzianum exhibits a broad spectrum of in vitro antibacterial activity against two strains of bacteria. For the optimum production of bioactive metabolites, Dextrose and Glucose were found to be the best sources of carbon and the best sources of Nitrogen Yeast extract (YE) and (NH4)2SO. The maximum production of bioactive metabolites occurs at pH 7 and 25°C.; the NaCl showed a positive influence on bioactive metabolites.

scholarly journals ANTI-MRSA OF THE ETHYL ACETATE CRUDE EXTRACT FROM LASIODIPLODIA PSEUDOTHEOBROMAE IBRL OS-64, AN ENDOPHYTIC FUNGUS ISOLATED FROM LEAF OF OCIMUM SANCTUM LINN

2018 ◽  
Vol 10 (8) ◽  
pp. 50 ◽  
Author(s):  
Taufiq M. M. J. ◽  
Darah I.
Keyword(s):  
Cell Wall ◽  
Cell Membrane ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Endophytic Fungus ◽  
Pathogenic Bacteria ◽  
Ocimum Sanctum ◽  
Minimal Bactericidal Concentration ◽  
Electron Microscopic ◽  
Time Kill

Objective: To investigate the effects of ethyl acetate crude extract of an endophytic fungus, L. pseudotheobromae IBRL OS-64 which was isolated from leaf of Ocimum sanctum against the growth of methicillin-resistant Staphylococcus aureus (MRSA)., a common pathogenic bacteria to human.Methods: Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined. Time-kill assay was used to examine the effect of the extract on the MRSA growth profile. The effects of the extract on the ultrastructure of MRSA cells were analyzed by scanning electron microscopic (SEM) study.Results: The results obtained from this study showed the fungal ethyl acetate crude extract exhibited a strong bactericidal effect on MRSA where the ratio of MBC/MIC was 2 and less than 4. The MIC and MBC values were 125.0 and 250.0 µg/ml, respectively. The time-kill study revealed that the bacteriocidal activity of the extract was both concentration and time-dependent. After 12 h treatment, the interaction of extract with MRSA cells resulted in the formation of dents, cavity or small dimples on the cell surface, indicating disintegration of the cell wall and cell membrane that resulting in leakage of their cytoplasmic contents, and ultimately cell death.Conclusion: The ethyl acetate crude extract of L. pseudotheobromae IBRL OS-64 showed a significant anti-MRSA activity and principally affected the cell wall and the cell membrane of the growing MRSA cells. This is the first report on L. pseudotheobromae, an endophytic fungus isolated from medicinal herb, Ocimum sanctum Linn.

Optimization of cultural conditions for tannase production by Pseudomonas aeruginosa IIIB 8914 under submerged fermentation

2009 ◽  
Vol 26 (4) ◽  
pp. 599-605 ◽  
Author(s):  
Manjit K. Selwal ◽  
Anita Yadav ◽  
Krishan K. Selwal ◽  
N. K. Aggarwal ◽  
Ranjan Gupta ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Submerged Fermentation ◽  
Cultural Conditions

scholarly journals Natural Red Pigment Production by Monascus Purpureus in Submerged Fermentation Systems Using a Food Industry Waste: Brewer’s Spent Grain

Foods ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 161 ◽  
Author(s):  
Selim Silbir ◽  
Yekta Goksungur
Keyword(s):  
Photoelectron Spectroscopy ◽  
Submerged Fermentation ◽  
Monosodium Glutamate ◽  
Fermentation Medium ◽  
Pigment Production ◽  
Monascus Purpureus ◽  
Red Pigment ◽  
Fermentation System ◽  
Brewer’S Spent Grain ◽  
Spent Grain

This paper studies the production of natural red pigments by Monascus purpureus CMU001 in the submerged fermentation system using a brewery waste hydrolysate, brewer’s spent grain (BSG). The chemical, structural and elemental characterization of the BSG was performed with Van-Soest method, Fourier-transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), respectively. The lignocellulosic structure of BSG was hydrolyzed with a dilute sulfuric acid solution (2% (w/v)) followed by detoxification with Ca(OH)2. Maximum red pigment production (22.25 UA500) was achieved with the following conditions: 350 rpm shake speed, 50 mL fermentation volume, initial pH of 6.5, inoculation ratio of 2% (v/v), and monosodium glutamate (MSG) as the most effective nitrogen source. Plackett–Burman design was used to assess the significance of the fermentation medium components, and MSG and ZnSO4·7H2O were found to be the significant medium variables. This study is the first study showing the compatibility of BSG hydrolysate to red pigment production by Monascus purpureus in a submerged fermentation system.

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