Optimization of cultural conditions affecting improved bioactive metabolite production by endophytic fungus Trichoderma harzianum

Bionatura ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 2187-2192
Author(s):  
Rashid Rahim Hateet ◽  
Zainab Alag Hassan ◽  
Abdulameer Abdullah Al-Mussawi ◽  
Shaima Rabeea Banoon
Keyword(s):  
Antibacterial Activity ◽  
Trichoderma Harzianum ◽  
Endophytic Fungus ◽  
Nitrogen Sources ◽  
Bioactive Metabolites ◽  
Bioactive Metabolite ◽  
Metabolite Production ◽  
Surface Sterilization ◽  
Cultural Conditions

The present study aimed to optimize cultural conditions for optimum bioactive metabolite production by endophytic fungus Trichoderma harzianum, isolated by surface sterilization method from the leaf of the eucalyptus plant. The fungus was identified based on morphological characterization. Fungal metabolites were carried out by ethyl acetate solvent. The antibacterial activity was tested against Escherichia coli (ATCC 25922) and Staphylococcus aureus (NCTC 6571). Various carbon, nitrogen sources, pH, temperature, incubation period, and NaCl on the antibacterial metabolite production were studied. Bioactive metabolite production of T. harzianum exhibits a broad spectrum of in vitro antibacterial activity against two strains of bacteria. For the optimum production of bioactive metabolites, Dextrose and Glucose were found to be the best sources of carbon and the best sources of Nitrogen Yeast extract (YE) and (NH4)2SO. The maximum production of bioactive metabolites occurs at pH 7 and 25°C.; the NaCl showed a positive influence on bioactive metabolites.

scholarly journals PRODUCTION, PURIFICATION, AND CHARACTERIZATION OF BIOACTIVE METABOLITES PRODUCED FROM RARE ACTINOBACTERIA PSEUDONOCARDIA ALNI

2016 ◽  
Vol 9 (9) ◽  
pp. 264
Author(s):  
Rabab Omran Aljelawi ◽  
Mohammed Fadhil Kadhem
Keyword(s):  
Culture Media ◽  
Nitrogen Sources ◽  
Bioactive Metabolites ◽  
Microsporum Canis ◽  
Agitation Rate ◽  
Potato Dextrose Broth ◽  
Metabolite Production ◽  
Antibacterial And Antifungal Activities ◽  
Cultural Conditions ◽  
Antibacterial And Antifungal

ABSTRACTObjectives: Pseudonocardia alni exhibits antimicrobial activity against tested pathogenic Staphylococcus aureus, Microsporum canis, and Trichophytonmentagrophyte. The present paper aimed to optimize various cultural conditions for antimicrobial metabolite production, purification, andcharacterization of the active substance.Methods: The effects of various parameters such as culture media, carbon and nitrogen sources, phosphate concentration, pH, temperature, incubationperiod, and agitation rate on bioactive metabolite production were studied using a flask scale with varying single parameter. The active substanceswere purified by adsorption chromatography using Silica gel column and Sephadex LH 20 column, and the physical, chemical, and biological propertieswere characterized.Results: The metabolite production by P. alni was greatly influenced by various cultural conditions. It produced high levels of the antimicrobialsubstance in International Streptomyces project-2 broth compared with that in potato dextrose broth. The optimum parameters for antimicrobialproduction from the actinobacterium occurred in the production medium consisting of glucose (1%) and tryptone (1%), 0.001 M of K2HPO4 and0.05M glycine at initial pH 8.5 and incubated at 30°C for 4 d in stand incubator. The higher concentration of phosphate buffer salts (˃0.01M) repressedthe bioactive production. The purified active substance had relative factor Rf=0.53 in the mobile phase of a thin layer chromatography system andthe maximum absorbance (λ max) at 216 nm. The results of infrared spectra Fourier transform-infrared spectroscopy analysis indicate that it may beregarded to glycopeptide antibiotic. The purified substance had antibacterial and antifungal activities as well as cytotoxic activity in breast cancer cellline Michigan Cancer Foundation-7 and normal hepatic cell line (WRL-68) at a percentage up to 23.7% and 7.64%, respectively.Conclusions: The actinobacterium P. alni was a novel strain having the ability to produce antimicrobial and anticancer substances.Keywords: Pseudonocardia alni, Antifungal–antibacterial, Anticancer optimization, Production, Purification, Characterization.

scholarly journals New Bioactive Sesquiterpeniods From the Plant Endophytic Fungus Pestalotiopsis theae

2021 ◽  
Vol 12 ◽  
Author(s):  
Gaoran Liu ◽  
Ruiyun Huo ◽  
Yanan Zhai ◽  
Ling Liu
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Cell Lines ◽  
Crude Extract ◽  
Endophytic Fungus ◽  
Spectroscopic Data ◽  
Mcf7 Cell ◽  
Cytotoxic Effects ◽  
Electronic Circular Dichroism

Three new secondary metabolites pestalothenins A–C (1–3), including two new humulane-derived sesquiterpeniods (1 and 2) and one new caryophyllene-derived sesquiterpeniod (3), together with five known compounds (4–8) were isolated from the crude extract of the plant endophytic fungus Pestalotiopsis theae (N635). Their structures were elucidated by the extensive analyses of HRESIMS and NMR spectroscopic data. The absolute configurations of 1–3 were determined by comparison of experimental and calculated electronic circular dichroism (ECD) spectra. The cytotoxic effects of these compounds were evaluated in vitro. Compound 6 showed moderate cytotoxicity against T24 and MCF7 cell lines. In addition, compounds 1–8 were also evaluated for antibacterial activity.

Trends of pharmaceutical design of Endophytes as anti-infective

2021 ◽  
Vol 21 ◽  
Author(s):  
Pragya Tiwari ◽  
Yashdeep Srivastava ◽  
Hanhong Bae
Keyword(s):  
Drug Discovery ◽  
Meta Analysis ◽  
Cost Effective ◽  
Bioactive Metabolites ◽  
Bioactive Metabolite ◽  
Metabolite Production ◽  
Drug Discovery Program ◽  
Life Threatening ◽  
Therapeutic Properties ◽  
Targeted Approach

: Plant-endophyte associations represent an inexhaustible source of novel metabolites, exhibiting significance in environment, agriculture and pharmaceutical perspectives. The global outbreak of life threatening diseases necessitate a need for a more targeted approach through efficient drug-discovery programs. In recent times, endophytes as “bio-factories” have been extensively explored for the production of novel, bioactive metabolites demonstrating therapeutic properties. Resources in computational biology co-integrated with combinational chemistry have made significant contributions in this field, aiding in the discovery and screening of potential “drug-like” molecules from endophytes. The review provides a meta-analysis of bioactive metabolite production from endophytes, extensively discussing the bio-prospection of natural products for pharmaceutical applications. In light of the emerging importance of endophytes as anti-infective agents, an exploration of the pharmaceutical design of novel chemical entities and analogues has enabled efficient and cost-effective drug discovery programs. However, bottlenecks in endophyte biology and research require a better understanding of endophyte dynamics and mechanism of bioactive metabolite production towards a sustainable drug discovery program.

scholarly journals Bioactive Compounds from Aspergillus Terreus MP15, an Endophytic Fungus Isolated from Swietenia Macrophylla Leaf

2017 ◽  
Vol 4 (2) ◽  
pp. 107-116
Author(s):  
Olivia Chan Jade Yin ◽  
Darah Ibrahim ◽  
Chong Chai Lee
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Endophytic Fungus ◽  
Aspergillus Terreus ◽  
Ethyl Acetate Extract ◽  
Bioactive Metabolites ◽  
Partial Purification ◽  
Swietenia Macrophylla ◽  
Gram Positive

Endophytes are important sources for the discovery of bioactive compounds. They are known as potential producers of novel compounds with antimicrobial activity. In this study, it aimed to study the bioactive metabolites present in the ethyl acetate extract from the endophytic fungus Aspergillus terreus MP15 of Swietenia macrophylla leaf. The ethyl acetate extract showed promising antibacterial activity against Gram-positive foodborne bacteria (Bacillus cereus, Bacillus spizizenii, Bacillus subtilis and Staphylococcus aureus) in disc diffusion assay. The extract was then subjected to thin layer chromatography (TLC) using dichloromethane: methanol (9:1) as a mobile solvent system and eleven spots with diverse polarities were obtained. The TLC chromatogram with bioactive spot was localized with bioautography assay and the result showed that the yellow spot with Rf value of 0.523 exhibited promising antibacterial activity against the four Gram-positive bacteria tested. Upon partial purification of the yellow bioactive fraction using column chromatography, the fraction 2a exhibited promising antibacterial activity. The GC-MS analysis of the yellow fraction 2a resulted in the detection of a major compound, di-n-octyl phthalate with 80% matching factor. Therefore, this compound may largely contribute to the antibacterial activity of the fraction and has potential to be food preservative and as a colorant. These results indicate that endophytic fungi isolated from medicinal plants could be a potential source for bioactive compounds.

scholarly journals Ameliorated Antibacterial and Antioxidant Properties by Trichoderma harzianum Mediated Green Synthesis of Silver Nanoparticles

Biomolecules ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 535
Author(s):  
Narasimhamurthy Konappa ◽  
Arakere C. Udayashankar ◽  
Nirmaladevi Dhamodaran ◽  
Soumya Krishnamurthy ◽  
Shubha Jagannath ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Trichoderma Harzianum ◽  
Antioxidant Properties ◽  
Xrd Analysis ◽  
Bioactive Metabolites ◽  
Infrared Spectrometry ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Average Size

Biosynthesis of silver nanoparticles using beneficial Trichoderma harzianum is a simple, eco-friendly and cost-effective route. Secondary metabolites secreted by T. harzianum act as capping and reducing agents that can offer constancy and can contribute to biological activity. The present study aimed to synthesize silver nanoparticles using T. harzianum cell filtrate and investigate different bioactive metabolites based on LC-MS/MS analysis. The synthesized silver nanoparticles (AgNPs) from T. harzianum were characterized by ultraviolet–visible spectrophotometry, Fourier transform infrared spectrometry (FT-IR), energy-dispersive spectroscopy (EDS), dynamic light scattering (DLS), X-ray powder diffraction (XRD) and scanning electron microscopy (SEM). The surface plasmon resonance of synthesized particles formed a peak centered near 438 nm. The DLS study determined the average size of AgNPs to be 21.49 nm. The average size of AgNPs was measured to be 72 nm by SEM. The cubic crystal structure from XRD analysis confirmed the synthesized particles as silver nanoparticles. The AgNPs exhibited remarkable antioxidant properties, as determined by DPPH and ferric reducing antioxidant power (FRAP) assay. The AgNPs also exhibited broad-spectrum antibacterial activity against two Gram-positive bacteria (S. aureus and B. subtilis) and two Gram-negative bacteria (E. coli and R. solanacearum). The minimum inhibitory concentration (MIC) of AgNPs towards bacterial growth was evaluated. The antibacterial activity of AgNPs was further confirmed by fluorescence microscopy and SEM analysis.

scholarly journals Antimicrobial activity of endophytic fungus Fusarium sp. isolated from medicinal honeysuckle plant

2016 ◽  
Vol 68 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Huiru Zhang ◽  
Xinchen Sun ◽  
Chunping Xu
Keyword(s):  
Antimicrobial Activity ◽  
Endophytic Fungus ◽  
Pathogenic Bacteria ◽  
New Drugs ◽  
Bioactive Metabolites ◽  
Gel Chromatography ◽  
Physiological Characterization ◽  
Wide Range ◽  
Fusarium Sp

Endophytes of plants have a wide range of strains comprising important sources of various bioactive metabolites. An endophytic fungus was isolated from honeysuckle, an important Chinese medicinal plant. The phylogenetic and physiological characterization indicated that the isolated strain JY2corresponded to Fusarium sp. The culture filtrate of JY2displayed antagonism activity against some pathogenic bacteria owing to the existence of antimicrobial compounds. The filtrate revealed the strongest in vitro antimicrobial activity on Pseudomonas aeruginosa by increasing the permeability of cell membranes. The antibacterial extract was fractionated and purifiedusing silica gel chromatography. Five different bioactive compounds were isolated by bioactivity-guided fractionation from the culture extracts of JY2, and preliminarily identified by HPLC-MS spectral data. These results suggest that Fusarium sp. features a potentially remarkable antimicrobial activity and could be valuable to discover the new drugs or agents for antimicrobial purposes.

Evaluation of In Vitro Antibacterial Activity of Endophytic Fungus from Anemone tomentosa

2014 ◽  
Vol 881-883 ◽  
pp. 484-487 ◽  
Author(s):  
Lin Yang ◽  
Jing Long ◽  
Wei Juan Li ◽  
Ai Mei Yang ◽  
Zhong Duo Yang ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Filter Paper ◽  
Endophytic Fungus ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Paper Method

In this study, 23 endophytic fungus were isolated from Anemone tomentosa. The antibacterial activity of the endophytic fungi were tested and analyzed by filter paper method. The results showed that the endophytic fungi from Anemone tomentosa had broad antibiogram and strong inhibited Gram-positive bacteria and Gram-negative bacteria, especially the mycelia extract of root-1, the ethyl acetate and the n-BuOH extracts of root-8. So the endophytic fungi from Anemone tomentosa could be an important resource for developing antimicrobial products.

scholarly journals Antibacterial Activity of Phytochemical Extracts and Endophytic Fungi of Carapa Guianensis Against Enterococcus Faecalis in Endodontic Infections An In Vitro Study

2019 ◽  
Vol 13 (1) ◽  
pp. 249-254 ◽  
Author(s):  
Maria do Carmo M. de Miranda ◽  
Clarice M. Carvalho ◽  
Fernando S.E.D.V. Faria ◽  
Antonio M-S. Nobreza ◽  
Thiago M. Pereira ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Enterococcus Faecalis ◽  
Endophytic Fungi ◽  
Bacterial Growth ◽  
Endophytic Fungus ◽  
Diffusion Method ◽  
Carapa Guianensis ◽  
Stem Extract

Objective: The objective of this study was to evaluate the antibacterial activity of phytochemical extracts and endophytic fungi of Carapa guianensis against Enterococcus faecalis. Carapa guianensis leaves and stems were collected to obtain phytochemical extracts and fungal metabolites and evaluated for in vitro antibacterial activity against E. faecalis using the disc diffusion method and dentin blocks with bacterial biofilm. Methods: Thirty dentin blocks were prepared and contaminated for 60 days with E. faecalis. The specimens were randomly divided into 6 experimental groups according to the test solution used: G1 – hexane stem extract of Carapa guianensis; G2 – methanol stem extract of Carapa guianensis; G3 – methanol leaf extract of Carapa guianensis; G4 – ethyl acetate extract of the endophytic fungus Penicillium isolated from Carapa guianensis; G5 – negative control, with no addition of bacterial inoculum; G6 – positive control. Results: Bacterial growth was analyzed by spectrophotometry after 14 days of direct contact between the extracts and dentin blocks. The hexane-stem, methanol-stem, methanol-leaf, and ethyl-acetate endophytic fungus Penicillium extracts inhibited bacterial growth in 100% of the samples. Conclusion: The present study demonstrated the antibacterial potential of phytochemical extracts and endophytic fungi of Carapa guianensis against E. faecalis.

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