scholarly journals Analysis of Vannamei shrimp DNA fragment resistant to White Spot Virus Syndrome

2021 ◽  
Vol 4 (1) ◽  
pp. 31-40
Author(s):  
Wiwien Mukti Andriyani ◽  
Sumini Sumini ◽  
Zeny Widiastuti
Keyword(s):  
Quality Improvement ◽  
High Potential ◽  
White Shrimp ◽  
Post Challenge ◽  
Spot Virus ◽  
Pcr Products ◽  
Dna Fragment ◽  
High Chance ◽  
Shrimp Cultivation ◽  
Rapd Method

The attack of WSSV in Vannamei shrimp cultivation is still common. Shrimp quality improvement can be made through selection with the help of markers (marker-assisted choice). This study aimed to evaluate the DNA fragment profile of white shrimp that was resistant to WSSV disease. The analysis was performed using the PCR-RAPD method. WSSV challenged four groups of 100 Vannamei shrimp, then DNA was extracted from live and dead shrimp. The results showed that 2 of the 17 primers tested had high potential as markers, namely OP-09 and OPD-2. PCR products with OPC-09 primers had specific DNA bands measuring about 1.2 kb in all post-challenge WSSV resistant shrimp individuals. The amplification results using OPD-02 primers showed a particular band of DNA with a length of about 1.0 kb, with 60 % of the appearance in WSSV-resistant shrimp. In contrast, the WSSV-susceptible shrimp group did not have specific DNA fragments. Thus, the two RAPD primers had a high chance of being used in the selection with the help of markers to produce WSSV resistant shrimp.

Effect of Brachionus plicatilis and Navicula sp. on Pacific white shrimp growth performance, Vibrio, immunological responses and resistance to white spot virus (WSSV) in nursery biofloc system

Aquaculture ◽  
2021 ◽  
pp. 736335
Author(s):  
Allyne Elins Moreira da Silva ◽  
Luis Otavio Brito ◽  
Danielle Alves da Silva ◽  
Priscilla Celes Maciel de Lima ◽  
Renata da Silva Farias ◽  
...  
Keyword(s):  
Growth Performance ◽  
Brachionus Plicatilis ◽  
Pacific White Shrimp ◽  
White Spot ◽  
White Shrimp ◽  
Spot Virus ◽  
Immunological Responses

scholarly journals Using PCR to Distinguish Diaporthe phaseolorum and Phomopsis longicolla from Other Soybean Fungal Pathogens and to Detect Them in Soybean Tissues

Plant Disease ◽  
1997 ◽  
Vol 81 (10) ◽  
pp. 1143-1149 ◽  
Author(s):  
A. W. Zhang ◽  
G. L. Hartman ◽  
L. Riccioni ◽  
W. D. Chen ◽  
R. Z. Ma ◽  
...  
Keyword(s):  
Fungal Pathogens ◽  
Nuclear Ribosomal Dna ◽  
Specific Primers ◽  
Phomopsis Longicolla ◽  
Tissue Samples ◽  
Diaporthe Phaseolorum ◽  
Pcr Products ◽  
Fungal Dna ◽  
Dna Fragment ◽  
Polymerase Chain

Restriction fragment length polymorphism analyses of polymerase chain reaction (PCR) amplified DNA were used to distinguish Diaporthe phaseolorum and Phomopsis longicolla isolates from other soybean fungal pathogens. Primers made to the conserved sequences of nuclear ribosomal DNA amplified the internal transcribed spacer (ITS) regions of D. phaseolorum var. meridionalis and P. longicolla. The PCR products were cloned and then sequenced. Specific-primers, Phom.I and Phom.II, were designed from the polymorphic regions of D. phaseolorum and P. longicolla isolates from soybean to distinguish them from other soybean fungal pathogens. These ITS-derived primers amplified a 337-bp-specific DNA fragment from P. longicolla, D. phaseolorum var. meridionalis, D. phaseolorum var. caulivora, D. phaseolorum var. sojae, and Phomopsis spp. from 20 different hosts. No amplified product was observed using DNA of seven other soybean fungal pathogens or soybean DNA. The detection limit of PCR using primers Phom.I and Phom.II was 2.5 × 10-7 dilution of fungal DNA extracted from samples of 10 pooled seeds and as low as a 1:15 (Phomopsis:soybean) ratio when using 10 ng of DNA per μl from each P. longicolla and soybean. PCR did not produce products using primers Phom.I and Phom.II with DNA extracted from noninfected seeds, but specific bands were observed from samples of 10 pooled seeds and from individually infected seeds. A specific band was observed as well from DNA extracts of tissue samples from symptomless plants inoculated with P. longicolla and D. phaseolorum var. sojae.

scholarly journals Development of Specific Nano-Antibody for Application in Selective and Rapid Environmental Diagnoses of Salmonella arizonae

2020 ◽  
Vol 10 (6) ◽  
pp. 7198-7208
Keyword(s):  
Sandwich Elisa ◽  
Economic Value ◽  
Bacterial Strains ◽  
Western Blot Assay ◽  
Phage Display Technique ◽  
Sds Page ◽  
Diagnostic Agents ◽  
Pcr Products ◽  
Dna Fragment ◽  
Display Technique

Treatment of human and animals for protection from pathogens infection has significant economic value, especially with the harmful Salmonella arizonae. Beneficial cameloid heavy-chain antibodies as single-domain antigen-binding fragments known as VHHs or nano-bodies may be the acceptable option for producing the treatment and/or diagnostic agents. In the current study, we developed a sandwich ELISA based nano-body towards S. arizonae as the first report for the treatment of S. arizonae. using the cDNA synthesized from immunized camels RNA to isolate 700 bp DNA fragment, which contains all VH domains of IgG2 and IgG3 isotypes followed by the second amplification VHH PCR with amplified fragments at 450 bp. The final PCR products were cloned into the phagemid vector pMECS then via phage display technique. Nano-antibodies protein was purified and separated under non-denaturing conditions by SDS-PAGE. The reactivity of each VHH of the selected clones was analyzed by Western blot assay. The isolated nano-antibodies showed binding not only to S. arizonae, but also to other bacterial strains, indicating that these nano-antibodies can be used in treatment but cannot use in diagnostic.

scholarly journals Glyceraldehyde-3-Phosphate Dehydrogenase-Encoding Gene as a Useful Taxonomic Tool for Staphylococcusspp.

2000 ◽  
Vol 38 (12) ◽  
pp. 4351-4355 ◽  
Author(s):  
Javier Yugueros ◽  
Alejandro Temprano ◽  
Beatriz Berzal ◽  
Marı́a Sánchez ◽  
Carmen Hernanz ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Fragment Length Polymorphism ◽  
Pcr Amplification ◽  
Rapid Identification ◽  
Pcr Products ◽  
Dna Fragment ◽  
Encoding Gene ◽  
Staphylococcus Hominis ◽  
Staphylococcus Simulans ◽  
Very High

The gap gene of Staphylococcus aureus, encoding glyceraldehyde-3-phosphate dehydrogenase, was used as a target to amplify a 933-bp DNA fragment by PCR with a pair of primers 26 and 25 nucleotides in length. PCR products, detected by agarose gel electrophoresis, were also amplified from 12 Staphylococcusspp. analyzed previously. Hybridization with an internal 279-bp DNA fragment probe was positive in all PCR-positive samples. No PCR products were amplified when other gram-positive and gram-negative bacterial genera were analyzed using the same pair of primers.AluI digestion of PCR-generated products gave 12 different restriction fragment length polymorphism (RFLP) patterns, one for each species analyzed. However, we could detect two intraspecies RFLP patterns in Staphylococcus epidermidis,Staphylococcus hominis, and Staphylococcus simulans which were different from the other species. An identical RFLP pattern was observed for 112 S. aureusisolates from humans, cows, and sheep. The sensitivity of the PCR assays was very high, with a detection limit for S. aureuscells of 20 CFU when cells were suspended in saline. PCR amplification of the gap gene has the potential for rapid identification of at least 12 species belonging to the genusStaphylococcus, as it is highly specific.

Effect of brown seaweeds on Pacific white shrimp growth performance, gut morphology, digestive enzymes activity and resistance to white spot virus

Aquaculture ◽  
2018 ◽  
Vol 495 ◽  
pp. 359-365 ◽  
Author(s):  
Delano Dias Schleder ◽  
Luiz Guilherme Buglione Peruch ◽  
Moisés Angel Poli ◽  
Tamiris Henrique Ferreira ◽  
Carlos Peres Silva ◽  
...  
Keyword(s):  
Growth Performance ◽  
Digestive Enzymes ◽  
Pacific White Shrimp ◽  
White Spot ◽  
White Shrimp ◽  
Spot Virus ◽  
Gut Morphology ◽  
Brown Seaweeds ◽  
Enzymes Activity

scholarly journals Molecular Cloning and Sequencing of thearoA Gene from Actinobacillus pleuropneumoniaeand Its Use in a PCR Assay for Rapid Identification

1999 ◽  
Vol 37 (5) ◽  
pp. 1575-1578 ◽  
Author(s):  
Carmen Hernanz Moral ◽  
Alberto Cascón Soriano ◽  
María Sánchez Salazar ◽  
Javier Yugueros Marcos ◽  
Susana Suárez Ramos ◽  
...  
Keyword(s):  
Rapid Identification ◽  
Chromosomal Dna ◽  
Pcr Assay ◽  
Specific Pcr ◽  
Specificity And Sensitivity ◽  
Pcr Products ◽  
Dna Fragment ◽  
Specific Pcr Assay ◽  
Dna Specificity ◽  
K 12

The gene (aroA) of Actinobacillus pleuropneumoniae, serotype 2, encoding 5-enolpyruvylshikimate-3-phosphate synthase was cloned by complementation of the aroA mutation in Escherichia coli K-12 strain AB2829, and the nucleotide sequence was determined. A pair of primers from the 5′ and 3′ termini were selected to be the basis for development of a specific PCR assay. A DNA fragment of 1,025 bp was amplified from lysed A. pleuropneumoniaeserotypes 1 to 12 of biovar 1 or from isolated DNA. No PCR products were detected when chromosomal DNAs from other genera were used as target DNAs; however, a 1,025-bp DNA fragment was amplified whenActinobacillus equuli chromosomal DNA was used as a target, which could be easily differentiated by its NAD independence. The PCR assay developed was very sensitive, with lower detection limits of 12 CFU with A. pleuropneumoniae cells and 0.8 pg with extracted DNA. Specificity and sensitivity make this PCR assay a useful method for the rapid identification and diagnosis of A. pleuropneumoniae infections.

scholarly journals The Impact of Good Agriculture Practices (GAP) Implementation on Productivity of Pacific White Shrimp in Grabag District Purworejo Regency

Agro Ekonomi ◽  
2019 ◽  
Vol 29 (2) ◽  
pp. 335
Author(s):  
Asyifa Anandya ◽  
Jamhari Jamhari ◽  
Suhatmini Hardyastuti
Keyword(s):  
Pacific White Shrimp ◽  
Least Square ◽  
White Shrimp ◽  
Level Of Education ◽  
Central Java ◽  
The Pacific ◽  
The Impact ◽  
Province Level ◽  
Central Java Province ◽  
Shrimp Cultivation

Purworejo Regency is one of the centers of pacific white shrimp cultivation in Indonesia. One of the sub-districts in Purworejo Regency which successfully cultivates the pacific white shrimp is Grabag Sub District, who won the first place of shrimp cultivation competition in organization and institution performace assessment category for Central Java Province level and national first place winner in shrimp cultivation category. This research was located in the sub district of Grabag, Purworejo regency on October 2017-December 2017. The aims of this research are to determine the effect of human capital on the GAP implementation by the farmers and the effect of the GAP implementation on pacific white shrimp productivity. The number of respondents in this research was 75 farmers which were chosen by purposive sampling. This research used regression analysis with Two Stage Least Square model. The results of this research showed that (1) The GAP implementation was influenced by the level of education and farmers’ experiences, while there was no any effect influenced by the age on the GAP implementation, and (2) The GAP implementation of pacific white shrimp cultivation did not affect the pacific white shrimp productivity. However, the GAP implementation did not contradict the productivity. The environmentally friendly GAP implementation and quality oriented did not contradict the productivity of shrimp. Farmers still can implement the GAP without decreasing their productivity.

Improvement of "Premium Coffee" Production in SRIDONORETNO Association of Dampit District, Malang

2019 ◽  
pp. 51
Author(s):  
Sugeng Riyanto ◽  
Jhauharotul Muchlisyiyah ◽  
Septrial Arafat
Keyword(s):  
Quality Improvement ◽  
Mobile Devices ◽  
High Potential ◽  
Quality Improvement Activity ◽  
Coffee Production ◽  
Group Members ◽  
The City ◽  
Coffee Shops ◽  
Processing Group

Malang is regency that has a high potential of coffee production which is 7,703 tons every year. Of the several types of coffee producers, one of the well-known is Dampit District which since the Dutch era was known as a coffee producer and until now the Dampit District is still known as the first coffee producer. The coffee quality improvement activity has consistently been started by the SRIDONORETNO Association. Currently the SRIDONORETNO Association's coffee production results are directly absorbed by the community of coffee shops and lovers in Malang (± 80 shops in Malang) and several shops outside the city of Malang. Activities undertaken to support consistent quality and increase the production of premium coffee are simply educating the group to raise the number of members participating in the red picking program. Train group members to become quality controllers. Create a processing group by making mobile devices that can serve many groups.

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