scholarly journals Effect of Annonaceous Sp. Ethanolic Extract on Transforming Growth Factor- Β (TGF- Β) Expression on MCF-7 Cells Line

2021 ◽  
Vol 11 (6-S) ◽  
pp. 49-52
Author(s):  
Muhartono Muhartono ◽  
Suharyani Suharyani ◽  
Asep Sukohar ◽  
Arli Suryawinata ◽  
Silvia Andriani
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Block Design ◽  
Ethanolic Extract ◽  
Transforming Growth Factor Β ◽  
Potential Effect ◽  
Analytic Study ◽  
Anti Cancer ◽  
Randomized Complete Block Design ◽  
Mcf 7

Aims: To analyze effect of the Annonaceous sp. ethanolic extract to Transforming Growth Factor- β (TGF- β) expressions on MCF-7 cells line. Methods: This experimental analytic study used Randomized Complete Block Design (RCBD) with three repetitions. The extract was dissolved in 1 ml of DMSO with a concentration of 0.1%. Furthermore, dilution was made with a dose of 0 µg / ml (K); 25 µg / ml (P1); 50 µg / ml (P2); 100 µg / ml (P3); 200 µg / ml (P4). MCF-7 cells line were cultured using RPMI 1640 Medium with 80 – 90% confluent. The ethanolic extract of Annonaceous sp. was exposed to MCF-7 cells for 48 hours. Analyze Transforming Growth Factor- β (TGF- β) level using ELISA Methods with λ=405 nm. Results: The results of the ELISA analysis show that the ethanolic extract of Annonaceous sp. has a potential effect of decreasing TGF- β expression on the sample with treatment on some concentration (p< 0,05). Conclusion: The ethanolic extract of Annonaceous sp. has shown the potential effect to decreased of TGF- β level on MCF-7 cells line Keywords: Annonaceous sp ethanolic extract, TGF- β, MCF-7 cells line, anti-cancer

scholarly journals The Role of Transforming Growth Factor-β in the Regulation of Estrogen Receptor Expression in the MCF-7 Breast Cancer Cell Line1

Endocrinology ◽  
1997 ◽  
Vol 138 (4) ◽  
pp. 1498-1505 ◽  
Author(s):  
Adriana Stoica ◽  
Miguel Saceda ◽  
Amina Fakhro ◽  
Harrison B. Solomon ◽  
Bradley D. Fenster ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Growth Factor ◽  
Breast Cancer Cell ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Receptor Expression ◽  
Estrogen Receptor Expression ◽  
Mcf 7

scholarly journals All trans-retinoic acid acts synergistically with hydroxytamoxifen and transforming-growth factor β to stimulate apoptosis in MCF-7 breast cancer cells

2004 ◽  
Vol 183 (2) ◽  
pp. 395-404 ◽  
Author(s):  
D N Danforth
Keyword(s):  
Breast Cancer ◽  
Growth Factor ◽  
Protein Expression ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Transforming Growth Factor ◽  
Synergistic Effects ◽  
Transforming Growth Factor Β ◽  
Mcf 7

The anti-estrogen 4-hydroxytamoxifen (TAM) and vitamin A-related compounds, the retinoids, in combination act synergistically to inhibit growth of breast cancer cells in vitro and in vivo. To clarify the mechanism of this synergism, the effect of TAM and all trans-retinoic acid (AT) on proliferation of MCF-7 breast cancer cells was studied in vitro. TAM and AT acted synergistically to cause a time-dependent and dose-dependent inhibition of MCF-7 cell growth. In a temporally related manner, TAM+AT acted synergistically to downregulate Bcl-2 mRNA and Bcl-2 protein expression, and to stimulate apoptosis. TAM and AT each blocked cell cycle progression throughout 7 days of treatment but without any synergistic or additive effect on this process, indicating a selective synergism for apoptosis. The negative growth factor-transforming growth factor β (TGFβ) is secreted by these cells and was studied as a potential mediator of the synergistic effects of TAM+AT on apoptosis. TAM+AT acted synergistically to induce a fivefold increase in TGFβ1 secretion over 72 h. TGFβ1 alone had no apoptotic effects on these cells; however, TGFβ1 in combination with AT acted synergistically to inhibit growth, to downregulate Bcl-2 mRNA and Bcl-2 protein expression, and to stimulate apoptosis of these cells in a manner comparable with that noted for TAM+AT. The synergism of both TAM+AT and TGFβ1+AT for apoptosis was suppressed by estradiol. Co-incubation of TAM+AT with anti-TGFβ antibody did not block down-regulation of Bcl-2 protein expression or stimulation of apoptosis. The synergistic effects of TAM+AT on apoptosis therefore occur independently of TGFβ, although TGFβ may interact with AT in a novel manner to provide another important anti-proliferative mechanism for breast cancer cells.

scholarly journals The Ethanolic Extract of Annonaceous sp. to Inhibit Vascular Endothelial Growth Factor (VEGF) as Anticancer Modalities

2021 ◽  
Vol 5 (3) ◽  
pp. 669-674
Author(s):  
Muhartono Muhartono ◽  
Asep Sukohar ◽  
Khairun Nisa ◽  
Ratna Dewi Puspita Sari ◽  
Suharyani ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Ethanolic Extract ◽  
Potential Effect ◽  
Major Public Health Problem ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor ◽  
Vegf Level ◽  
Mcf 7

Introduction: Cancer has become a major public health problem worldwide, with breast cancer is the most common cancer among women and one of the most important causes of death among them. Management of breast cancer have some adverse effects, with many factorx=s shiouln be considered to ordered the therapy. Use of chemotherapy will suppress cell proliferation and trigger apoptosis, but it cannot just focus on cancer cells only, but also would affected on other cells and cause. Objective: This study aim to analyze the inhibition effect of the Annonanceous sp. ethanolic extract to Vascular Endothelial Growth Factor (VEGF) on MCF-7 cells line Material and Methods: This study is an analytic experimental study used Randomized Complete Block Design (RCBD) with three repetitions. The extract was dissolved in 1 ml of DMSO with a concentration of 0.1%. Furthermore, dilution was made with a dose of 0 µg / ml (K); 25 µg / ml (P1); 50 µg / ml (P2); 100 µg / ml (P3); 200 µg / ml (P4). MCF-7 cells line were cultured used RPMI 1640 Medium with 80 – 90% confluent. The ethanolic extract of Annonaceous sp. were exposed to MCF-7 cells for 48 hours. Analyze of VEGF level use ELISA Methods with λ=405 nm. Results: The results of ELISA analyze shows that the ethanolic extract of Annonaceous sp. have potential effect to decreased of VEGF expression on sample with treatment on some concentration (p< 0,05). Conclusion: The ethanolic extract of Annonaceous sp. has shows the potential effect to decreased of VEGF level on MCF-7 cells line

scholarly journals The Ethanolic Extract of Annonaceous sp. to Inhibit Vascular Endothelial Growth Factor (VEGF) as Anticancer Modalities

2021 ◽  
Vol 5 (7) ◽  
pp. 642-646
Author(s):  
Muhartono Muhartono ◽  
Asep Sukohar ◽  
Khairun Nisa ◽  
Ratna Dewi Puspita Sari ◽  
Suharyani ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Ethanolic Extract ◽  
Potential Effect ◽  
Major Public Health Problem ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor ◽  
Vegf Level ◽  
Mcf 7

Introduction: Cancer has become a major public health problem worldwide, with breast cancer is the most common cancer among women and one of the most important causes of death among them. Management of breast cancer have some adverse effects, with many factorx=s shiouln be considered to ordered the therapy. Use of chemotherapy will suppress cell proliferation and trigger apoptosis, but it cannot just focus on cancer cells only, but also would affected on other cells and cause. Objective: This study aim to analyze the inhibition effect of the Annonanceous sp. ethanolic extract to Vascular Endothelial Growth Factor (VEGF) on MCF-7 cells line Material and Methods: This study is an analytic experimental study used Randomized Complete Block Design (RCBD) with three repetitions. The extract was dissolved in 1 ml of DMSO with a concentration of 0.1%. Furthermore, dilution was made with a dose of 0 µg / ml (K); 25 µg / ml (P1); 50 µg / ml (P2); 100 µg / ml (P3); 200 µg / ml (P4). MCF-7 cells line were cultured used RPMI 1640 Medium with 80 – 90% confluent. The ethanolic extract of Annonaceous sp. were exposed to MCF-7 cells for 48 hours. Analyze of VEGF level use ELISA Methods with λ=405 nm. Results: The results of ELISA analyze shows that the ethanolic extract of Annonaceous sp. have potential effect to decreased of VEGF expression on sample with treatment on some concentration (p< 0,05). Conclusion: The ethanolic extract of Annonaceous sp. has shows the potential effect to decreased of VEGF level on MCF-7 cells line

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