scholarly journals Effect of Time and Haemolysis on Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT) Measurement on Blood Samples

2021 ◽  
Vol 11 (6-S) ◽  
pp. 114-122
Author(s):  
Tahia Jafar Abdo Alhakam Eshag ◽  
Maye M. Merghani ◽  
Nihad Elsadig Babiker
Keyword(s):  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Blood Clot ◽  
Activated Partial Thromboplastin Time ◽  
Medical Laboratory ◽  
Control Group ◽  
Case Group ◽  
Age And Gender ◽  
Blood Samples ◽  
And Gender

Background: Coagulation, also known as blood clotting, is the process by which blood convert from a liquid to a gel, forming a blood clot. It referred to haemostasis, the stopping of blood loss from a damaged vessel, followed by repair. Material and methods: This was cross sectional study conducted at the albawasla medical laboratory, Khartoum, Sudan during the period August to November, 2021 and to evaluate the effect of time and hemolysis on prothrombin time and activated partial thromboplastin time tests. 50 samples (case group) were collected from the patients attending police teaching hospital   and requested to the PT and APTT test in addition to that,50 apparently healthy donors with no history of any coagulation problems or any chronic disease were selected as control group. Three ml of venous blood samples were collected in container with Tri Sodium Citrate anticoagulant. The coagulation tests (PT and APTT) were performed using semiautomatic device (coagulometer machine MI). Results:  The result of this study revealed that; when compared the measurement of PT and APTT immediately and after one hour there was insignificant differences (p. v.>0.05).  also when compared the measurement of PT and APTT between hemolyzed and non-hemolyzed samples there was significant differences ( p. v.<0.05)  in addition when compared case and control for the PT and APTT immediately,  after one hour, hemolyzed and non-hemolyzed sample there was significant differences ( p. v.<0.05) except the APTT hemolyzed samples  and  insignificant differences with age and gender ( p. v.>0.05).  For the correlation there was significant correlation in the case group for the PT and APTT immediately, after one hour, and hemolyzed samples. Conclusion: In the cases group results showed insignificant differences in the results of PT and APTT between immediate sample and after 1 hour in and significant differences in the results of PT and APTT between hemolyzed and non-hemolyzed samples, also there was insignificant differences between age and gender, immediately, after one hr. and hemolyzed sample in PT and APTT. Keywords:  Homeostasis,  hemolyzed  sample, PT and APTT

Effects of a Pneumatic Tube System on Routine and Novel Hematology and Coagulation Parameters in Healthy Volunteers

2007 ◽  
Vol 131 (2) ◽  
pp. 293-296
Author(s):  
Alexander Kratz ◽  
Raneem O. Salem ◽  
Elizabeth M. Van Cott
Keyword(s):  
Healthy Volunteers ◽  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Activated Partial Thromboplastin Time ◽  
Tube System ◽  
Blood Samples ◽  
Pneumatic Tube ◽  
The Mean ◽  
Clinically Significant ◽  
Pneumatic Tube System

Abstract Context.—Technologic advances affecting analyzers used in clinical laboratories have changed the methods used to obtain many laboratory measurements, and many novel parameters are now available. The effects of specimen transport through a pneumatic tube system on laboratory results obtained with such modern instruments are unclear. Objective.—To determine the effects of sample transport through a pneumatic tube system on routine and novel hematology and coagulation parameters obtained on state-of-the-art analyzers. Design.—Paired blood samples from 33 healthy volunteers were either hand delivered to the clinical laboratory or transported through a pneumatic tube system. Results.—No statistically significant differences were observed for routine complete blood cell count and white cell differential parameters or markers of platelet activation, such as the mean platelet component, or of red cell fragmentation. When 2 donors who reported aspirin intake were excluded from the analysis, there was a statistically, but not clinically, significant impact of transport through the pneumatic tube system on the mean platelet component. There were no statistically significant differences for prothrombin time, activated partial thromboplastin time, waveform slopes for prothrombin time or activated partial thromboplastin time, fibrinogen, or fibrin monomers. Conclusions.—Although further study regarding the mean platelet component may be required, transport through a pneumatic tube system has no clinically significant effect on hematology and coagulation results obtained with certain modern instruments in blood samples from healthy volunteers.

Accuracy of drawing coagulation samples from heparinized arterial lines

1993 ◽  
Vol 2 (1) ◽  
pp. 88-95 ◽  
Author(s):  
K Templin ◽  
M Shively ◽  
J Riley
Keyword(s):  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Coronary Angioplasty ◽  
Percutaneous Transluminal Coronary Angioplasty ◽  
Activated Partial Thromboplastin Time ◽  
Arterial Line ◽  
Blood Samples ◽  
Transluminal Coronary Angioplasty ◽  
Percutaneous Transluminal ◽  
Line Sample

OBJECTIVE: To determine the accuracy of activated partial thromboplastin time and prothrombin time studies when samples are drawn through heparinized arterial lines. METHODS: A total sample of 90 grouped blood samples (from 30 subjects) was used. Patients were all male, with a mean age of 65 and were studied within 24 hours of percutaneous transluminal coronary angioplasty. Each patient had three venous control and arterial line sample sets (a total of 90 blood samples) drawn when routinely ordered for monitoring therapy. For the arterial line sample, a discard volume of the deadspace, deadspace + 2 mL, or deadspace + 4 mL was randomly assigned for each sample. The venous control volumes were the same for all three sample sets. RESULTS: A 2 x 3 repeated measures analysis of variance was used to analyze the results. The independent variables were the source of the sample (venous vs arterial) and the discard volume of arterial blood (deadspace, deadspace + 2 mL, deadspace + 4 mL). The dependent variables were the activated partial thromboplastin time and prothrombin time values. Mean arterial activated partial thromboplastin time values were significantly higher than the corresponding venous values. Mean activated partial thromboplastin time values were not significantly different among the discard volumes of blood drawn. However, there was a significant source by volume interaction. Tukey post-hoc comparisons of venous-arterial activated partial thromboplastin time differences among the three volumes showed significant differences between deadspace volume and deadspace + 2 mL, and deadspace volume and deadspace + 4 mL. There was no significant difference between deadspace + 2 mL and deadspace + 4 mL volumes. CONCLUSION: Results indicated that the minimal amount of discard volume for accurate activated partial thromboplastin time values in this population of percutaneous transluminal coronary angioplasty patients was the catheter deadspace volume plus 2 mL (total 3.6 mL).

Evaluation of bone marrow with particular consideration of the megakaryocyte lineage and coagulation profile in the pregnant fallow deer (Dama dama)

2016 ◽  
Vol 19 (2) ◽  
pp. 359-364
Author(s):  
A. Snarska ◽  
P. Sobiech
Keyword(s):  
Bone Marrow ◽  
Platelet Count ◽  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Fallow Deer ◽  
Activated Partial Thromboplastin Time ◽  
Control Group ◽  
Slight Reduction ◽  
Thrombin Time ◽  
Coagulation Parameters

Abstract The aim of the study was to evaluate the megakaryocyte lineage of bone marrow and coagulation parameters in fallow deer during the last month of pregnancy. The animals were managed in the barn-feeding system. Twenty female fallow deer, aged 2-3 years, divided into 2 groups were used in the study. Group 1 comprised the females in the last month of pregnancy, and the non-pregnant females were used as the control. All the animals were clinically healthy. Coagulation parameters were measured in all the deer: thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT), and plasma concentrations of fibrinogen, D-dimer, and antithrombin III. A quantitative assessment of bone marrow was carried out for the erythroblastic, myeloid, lymphoid, monocyte-macrophage, and megakaryopoietic cell lines. A detailed analysis of megakaryocyte lineage was performed after whole blood and platelet count. There were no significant differences in the erythroblast, granulocyte, monocyte-macrophage and lymphoid systems between the animal groups. Thrombocyte count in the pregnant deer was lower than that found in the control group. Bone marrow smears revealed a slightly decreased megakaryocyte count, while the megakaryoblast and promegakaryocyte counts were unchanged. The analysis of coagulation parameters showed increased levels of fibrinogen, thrombin time, prothrombin time and activated partial thromboplastin time in the pregnant animals. The study suggested a hyperactivation of the coagulation system with a slight reduction in the megakaryocyte count in bone marrow, and a reduction in platelet count in peripheral blood at the end of pregnancy.

scholarly journals Assessment of Prothrombin Time, Activated Partial Thromboplastin Time, and Fibrinogen Concentration on Equine Plasma Samples following Different Storage Conditions

2009 ◽  
Vol 21 (5) ◽  
pp. 674-678 ◽  
Author(s):  
Stefania Casella ◽  
Claudia Giannetto ◽  
Francesco Fazio ◽  
Elisabetta Giudice ◽  
Giuseppe Piccione
Keyword(s):  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Repeated Measures ◽  
Storage Conditions ◽  
Activated Partial Thromboplastin Time ◽  
Clinical Samples ◽  
Fibrinogen Concentration ◽  
Experimental Conditions ◽  
Equine Plasma ◽  
And Gender

The aim of the present study was to assess the effect of different storage conditions on prothrombin time (PT), activated partial thromboplastin time (aPTT), and fibrinogen concentration in clinical samples from healthy horses. A total of 100 healthy horses of varying breeds and gender, ranging in age from 4 to 18 years, with a mean body weight of 480 + 70 kg, were used. Blood was collected by jugular venipuncture, and a hemochrome-cytometric examination was conducted on all samples. All blood samples were centrifuged and divided into 4 different aliquots to assess clotting parameters by means of a coagulometer. The first aliquots were analyzed 1 hr after collection, the second aliquots were refrigerated at 8°C for 6 hr, the third aliquots were frozen at −20°C for 24 hr, and the fourth aliquots were frozen at −20°C for 48 hr. Significant differences ( P < 0.05) were determined by one-way analysis of variance with repeated measures, and statistical analysis showed a significant effect of the experimental conditions on all parameters studied. In particular, the results demonstrated that coagulation tests can be done within 6 hr when samples are stored at 8°C because the short-term refrigeration does not change the result of analyses; storage at −20°C is acceptable only after 24 hr for PT, aPTT, and fibrinogen measurements because after 48 hr, freezing alters the values of clotting parameters. Therefore, the results of this investigation indicate that clotting parameters remain stable only up to 24 hr in horses without adversely affecting hemostasis test results.

scholarly journals Estimation of Fibrinogen Level among Sudanese Patients with Myocardial Infraction

2019 ◽  
Vol 9 (6-s) ◽  
pp. 21-27
Author(s):  
Mohammed AL Mustafa Ahmed Edres ◽  
Nihad Elsadig Babiker
Keyword(s):  
Prothrombin Time ◽  
Fibrinogen Level ◽  
Cross Sectional Study ◽  
Control Group ◽  
Case Group ◽  
Cross Sectional ◽  
High Risk Factors ◽  
Myocardial Infraction ◽  
Artery Disease ◽  
And Gender

Myocardial infraction (AMI) is an irreversible myocardial injury and necrosis caused by serious and long-term ischemia. It is generally seen in middle aged men with high risk factors for coronary artery disease. Only 4% of patients with AMI are under 40 years of age. This was a cross sectional study conducted at AL SHAB hospital, Khartoum, Sudan, aimed to estimate the fibrinogen level among Sudanese patients with myocardial infraction. 50 patients attending Alshab Hospital and diagnosed with MI used as a case group and 50 apparently healthy individuals with no history of MI were selected as control group. 1.8 ml of blood samples has been collected in sodium citrate anticoagulant container for measurement of fibrinogen level by clauss method in coagulometer device. It is clearly significant increase in fibrinogen level in myocardial infraction patient (p.value 0.000). In addition, the prothrombin time and gender were insignificantly differences in (AMI) patients. In the other hand, age and smoking significantly increased as risk factor in myocardial infraction. This study concluded that fibrinogen level was significantly increased in Sudanese patients with  myocardial infraction. Keywords: Myochardial Infraction, Fibrinogen, Prothrombin Time, Coagulometer

Comparison of PT and aPTT values drawn by venipuncture and arterial line using three discard volumes

1992 ◽  
Vol 1 (3) ◽  
pp. 94-101 ◽  
Author(s):  
E Konopad ◽  
M Grace ◽  
R Johnston ◽  
T Noseworthy ◽  
A Shustack
Keyword(s):  
Prothrombin Time ◽  
Critically Ill ◽  
Partial Thromboplastin Time ◽  
Laboratory Tests ◽  
Activated Partial Thromboplastin Time ◽  
Arterial Line ◽  
Bonferroni Correction ◽  
Blood Samples ◽  
Volume Group ◽  
Paired T Test

BACKGROUND: Blood samples obtained through heparinized arterial catheters are used routinely for a variety of laboratory tests. Accuracy of coagulation studies performed from samples obtained in this fashion continues to be questioned, particularly in regard to the minimum discard volume necessary to clear the catheter of heparinized solution. OBJECTIVE: To examine differences between prothrombin time and activated partial thromboplastin time values obtained from blood drawn by venipuncture and from an indwelling intra-arterial line using three discard volumes. METHODS: Prothrombin time and activated partial thromboplastin time samples were drawn by venipuncture from 41 critically ill adult patients. Simultaneously, three consecutive blood samples of 2.3 mL were drawn from the arterial line after an initial discard volume of 3 mL (discard volumes of 3.0, 5.3 and 7.6 mL). RESULTS: Significant differences were found between arterial and venous prothrombin time values for the 3-mL discard volume group, as well as between arterial and venous activated partial thromboplastin time values for all three discard volume groups (paired t-test, Bonferroni correction). CONCLUSION: We recommend that when drawing prothrombin time and activated partial thromboplastin time samples from an arterial line, a 5.3-mL discard volume be used.

scholarly journals Haemostatic Effects of Ethanolic Extracts of Amaranthus hybridus on Wistar Rats

2020 ◽  
pp. 14-21
Author(s):  
B. C. Chinko ◽  
F. S. Amah-Tariah
Keyword(s):  
Platelet Count ◽  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Wistar Rats ◽  
Activated Partial Thromboplastin Time ◽  
Control Group ◽  
Dependent Manner ◽  
Amaranthus Hybridus ◽  
Wide Range ◽  
Ethanolic Extracts

Introduction: Haemostasis refers to the arrest of bleeding due to vascular damage and involves the intrinsic and extrinsic coagulation pathways which converge at the point of fibrin activation to stop or minimize blood loss. Amaranthus hybridus contains a while range of nutritional, chemical and phytochemical constituents which gives it wide range of applications in folk medicine. Aim: To evaluate the effects of ethanolic extracts of Amaranthus hybridus on blood platelet count, prothrombin time (PT) and activated partial thromboplastin time (APTT) using Wistar rat models. Methodology: Twenty Four (24) adult male Wistar rats were used for the study. The animals were randomly divided into three (3) groups of eight (8) animals each. Oral administration of distilled water for the control group and ethanolic extracts of Amaranthus hybridus at 30 and 60 mg/kg lasted for twenty eight (28) days. Platelet count, prothrombin time (PT) and activated partial thromboplastin time (APTT) were determined using standard laboratory methods. Results: Ethanolic extracts of Amaranthus hybridus significantly increased platelet count at 30 mg and 60 mg/kg compared to the control animals (p<0.05). Also, it significantly reduced prothrombin time and activated partial thromboplastin time at 30 and 60 mg/kg in a dose dependent manner compared to control animals (P<0.05).   Conclusion: The study shows that ethanolic extract of Amaranthus hybridus may have enhanced haemostasis as demonstrated by increased platelet count and reduced prothrombin (PT) and activated partial thromboplastin time (APTT) time.

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