Effects of a Pneumatic Tube System on Routine and Novel Hematology and Coagulation Parameters in Healthy Volunteers

2007 ◽  
Vol 131 (2) ◽  
pp. 293-296
Author(s):  
Alexander Kratz ◽  
Raneem O. Salem ◽  
Elizabeth M. Van Cott
Keyword(s):  
Healthy Volunteers ◽  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Activated Partial Thromboplastin Time ◽  
Tube System ◽  
Blood Samples ◽  
Pneumatic Tube ◽  
The Mean ◽  
Clinically Significant ◽  
Pneumatic Tube System

Abstract Context.—Technologic advances affecting analyzers used in clinical laboratories have changed the methods used to obtain many laboratory measurements, and many novel parameters are now available. The effects of specimen transport through a pneumatic tube system on laboratory results obtained with such modern instruments are unclear. Objective.—To determine the effects of sample transport through a pneumatic tube system on routine and novel hematology and coagulation parameters obtained on state-of-the-art analyzers. Design.—Paired blood samples from 33 healthy volunteers were either hand delivered to the clinical laboratory or transported through a pneumatic tube system. Results.—No statistically significant differences were observed for routine complete blood cell count and white cell differential parameters or markers of platelet activation, such as the mean platelet component, or of red cell fragmentation. When 2 donors who reported aspirin intake were excluded from the analysis, there was a statistically, but not clinically, significant impact of transport through the pneumatic tube system on the mean platelet component. There were no statistically significant differences for prothrombin time, activated partial thromboplastin time, waveform slopes for prothrombin time or activated partial thromboplastin time, fibrinogen, or fibrin monomers. Conclusions.—Although further study regarding the mean platelet component may be required, transport through a pneumatic tube system has no clinically significant effect on hematology and coagulation results obtained with certain modern instruments in blood samples from healthy volunteers.

scholarly journals Correlation of prothrombin time and activated partial thromboplastin time with serum immunoglobulins in newly diagnosed patients with plasma cell dyscrasias: an experience from tertiary care centre

2018 ◽  
Vol 5 (2) ◽  
pp. 419
Author(s):  
Filza Shafi ◽  
Sajad Geelani ◽  
Javid Rasool ◽  
Subuh Parvez Khan ◽  
Syed Mudasir Qadri ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Prothrombin Time ◽  
Plasma Cell ◽  
Light Chain ◽  
Partial Thromboplastin Time ◽  
Tertiary Care ◽  
Activated Partial Thromboplastin Time ◽  
P Value ◽  
Newly Diagnosed ◽  
The Mean

Background: Plasma cell dyscrasia (PCD) is the term used to describe the disorders characterized by neoplastic proliferation of plasma cells with the abnormal production of immunoglobulins (Ig). Patients with multiple myeloma frequently have abnormal coagulation tests. Aim of the present study were to correlate prothrombin time (PT) and Activated Partial Thromboplastin time (aPTT) with Ig concentrations in patients with newly diagnosed with PCD and to compare PT and aPTT values in untreated and treated patients diagnosed with PCDMethods: This study was conducted in the department of clinical hematology of SKIMS, a tertiary care hospital in northern India from 2015 to 2016. Patients diagnosed with PCD were advised for coagulogram (PT, aPTT) as a base line investigation. A total of 72 patients were included in the study.Results: 37% of multiple myeloma cases (newly diagnosed) and 22% of light chain disease patients presented with prolonged PT whereas none of the patients in treated cases of PCD had prolonged PT. The mean Ig concentration was significantly higher in patients with prolonged PT and aPTT compared to that of patients with normal PT and aPTT values. In IgA myeloma, the mean immunoglobulin concentration was 3643 mg/dL with a mean PT and aPTT values of 18.8s and 36.6 (p value: 0.006). The mean free light chain concentration in kappa (k) light chain myeloma was 1727 mg/L with a mean PT value of 20.5 s, mean aPTT value of 37.4 s (p-value: 0.026).Conclusions: Patients with newly diagnosed myeloma presented with prolonged PT as compared to the treated cases. Also, mean Ig concentration was significantly higher in patients with prolonged PT and aPTT compared to that of patients with normal PT and aPTT values.

Accuracy of drawing coagulation samples from heparinized arterial lines

1993 ◽  
Vol 2 (1) ◽  
pp. 88-95 ◽  
Author(s):  
K Templin ◽  
M Shively ◽  
J Riley
Keyword(s):  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Coronary Angioplasty ◽  
Percutaneous Transluminal Coronary Angioplasty ◽  
Activated Partial Thromboplastin Time ◽  
Arterial Line ◽  
Blood Samples ◽  
Transluminal Coronary Angioplasty ◽  
Percutaneous Transluminal ◽  
Line Sample

OBJECTIVE: To determine the accuracy of activated partial thromboplastin time and prothrombin time studies when samples are drawn through heparinized arterial lines. METHODS: A total sample of 90 grouped blood samples (from 30 subjects) was used. Patients were all male, with a mean age of 65 and were studied within 24 hours of percutaneous transluminal coronary angioplasty. Each patient had three venous control and arterial line sample sets (a total of 90 blood samples) drawn when routinely ordered for monitoring therapy. For the arterial line sample, a discard volume of the deadspace, deadspace + 2 mL, or deadspace + 4 mL was randomly assigned for each sample. The venous control volumes were the same for all three sample sets. RESULTS: A 2 x 3 repeated measures analysis of variance was used to analyze the results. The independent variables were the source of the sample (venous vs arterial) and the discard volume of arterial blood (deadspace, deadspace + 2 mL, deadspace + 4 mL). The dependent variables were the activated partial thromboplastin time and prothrombin time values. Mean arterial activated partial thromboplastin time values were significantly higher than the corresponding venous values. Mean activated partial thromboplastin time values were not significantly different among the discard volumes of blood drawn. However, there was a significant source by volume interaction. Tukey post-hoc comparisons of venous-arterial activated partial thromboplastin time differences among the three volumes showed significant differences between deadspace volume and deadspace + 2 mL, and deadspace volume and deadspace + 4 mL. There was no significant difference between deadspace + 2 mL and deadspace + 4 mL volumes. CONCLUSION: Results indicated that the minimal amount of discard volume for accurate activated partial thromboplastin time values in this population of percutaneous transluminal coronary angioplasty patients was the catheter deadspace volume plus 2 mL (total 3.6 mL).

Effect of Fondaparinux on Coagulation Assays: Results of College of American Pathologists Proficiency Testing

2006 ◽  
Vol 130 (11) ◽  
pp. 1605-1611 ◽  
Author(s):  
Agata Smogorzewska ◽  
John T. Brandt ◽  
Wayne L. Chandler ◽  
Mark T. Cunningham ◽  
Timothy E. Hayes ◽  
...  
Keyword(s):  
Factor Viii ◽  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Factor Xa ◽  
Cost Effective ◽  
Activated Partial Thromboplastin Time ◽  
Thrombin Time ◽  
Standard Curve ◽  
Relevant Effect ◽  
Clinically Significant

Abstract Context.—Fondaparinux, a factor Xa inhibitor, is approved for thromboprophylaxis after orthopedic surgery and for treatment of venous thromboembolism. It may also be efficacious, safe, and cost-effective for other patients; thus, more widespread use of fondaparinux is likely. The effect of fondaparinux on coagulation testing needs to be thoroughly examined. Objective.—To report the effects of fondaparinux on coagulation tests (prothrombin time, activated partial thromboplastin time, fibrinogen, antithrombin, factor VIII, thrombin time, anti–factor Xa) across diverse methodologies. Design.—Samples with different concentrations of fondaparinux (0, 0.4, 0.8, and 2.0 μg/mL) were sent to laboratories participating in the College of American Pathologists Comprehensive Coagulation proficiency survey (N = 898). Laboratory-specific methods were used to assay coagulation parameters. Results.—Prophylactic or therapeutic fondaparinux prolonged the prothrombin time by approximately 1 second and the activated partial thromboplastin time by 4 to 5 seconds, and reduced factor VIII from 119% to 107% and 102%, respectively. Supratherapeutic fondaparinux reduced factor VIII to 85%. The activated partial thromboplastin time was prolonged in 19%, 29%, and 52% of laboratories with prophylactic, therapeutic, and supratherapeutic fondaparinux levels, respectively. Fibrinogen, antithrombin, and thrombin time assays did not show clinically significant changes. When measuring fondaparinux concentration using an anti–factor Xa assay, the most accurate results were obtained when fondaparinux was used as the calibrator. Conclusions.—Fondaparinux, even in prophylactic doses, slightly prolongs the prothrombin time and activated partial thromboplastin time and can interfere with factor VIII assays, but it has no clinically relevant effect on fibrinogen, antithrombin, or thrombin time. A fondaparinux standard curve should be used for reporting fondaparinux levels using an anti–factor Xa assay.

Comparison of pneumatic tube system with manual transport for routine chemistry, hematology, coagulation and blood gas tests

2017 ◽  
Vol 55 (10) ◽  
Author(s):  
Alex Pupek ◽  
Beverly Matthewson ◽  
Erin Whitman ◽  
Rachel Fullarton ◽  
Yu Chen
Keyword(s):  
Laboratory Tests ◽  
Capillary Tube ◽  
Statistical Significance ◽  
Test Results ◽  
Blood Gas ◽  
Tube System ◽  
Pneumatic Tube ◽  
Clinically Significant ◽  
Pneumatic Tube System ◽  
Tube Study

AbstractBackground:The pneumatic tube system (PTS) is commonly used in modern clinical laboratories to provide quick specimen delivery. However, its impact on sample integrity and laboratory testing results are still debatable. In addition, each PTS installation and configuration is unique to its institution. We sought to validate our Swisslog PTS by comparing routine chemistry, hematology, coagulation and blood gas test results and sample integrity indices between duplicate samples transported either manually or by PTS.Methods:Duplicate samples were delivered to the core laboratory manually by human courier or via the Swisslog PTS. Head-to-head comparisons of 48 routine chemistry, hematology, coagulation and blood gas laboratory tests, and three sample integrity indices were conducted on 41 healthy volunteers and 61 adult patients.Results:The PTS showed no impact on sample hemolysis, lipemia, or icterus indices (all p<0.05). Although alkaline phosphatase, total bilirubin and hemoglobin reached statistical significance (p=0.009, 0.027 and 0.012, respectively), all had very low average bias which ranged from 0.01% to 2%. Potassium, total hemoglobin and percent deoxyhemoglobin were statistically significant for the neonatal capillary tube study (p=0.011, 0.033 and 0.041, respectively) but no biases greater than ±4% were identified for these parameters. All observed differences of these 48 laboratory tests were not clinically significant.Conclusions:The modern PTS investigated in this study is acceptable for reliable sample delivery for routine chemistry, hematology, coagulation and blood gas (in syringe and capillary tube) laboratory tests.

scholarly journals Hemolysis Associated With Pneumatic Tube System Transport For Blood Samples

1969 ◽  
Vol 30 (1) ◽  
Author(s):  
Hasan Kara ◽  
Aysegul Bayir ◽  
Ahmet Ak ◽  
Selim Degirmenci ◽  
Murat Akinci ◽  
...  
Keyword(s):  
Tube System ◽  
Blood Samples ◽  
Pneumatic Tube ◽  
Pneumatic Tube System

Are TEG®results in healthy blood donors affected by the transport of blood samples in a pneumatic tube system?

2016 ◽  
Vol 38 (4) ◽  
pp. e73-e76 ◽  
Author(s):  
A. Espinosa ◽  
A. Ruckert ◽  
J. Navarro ◽  
V. Videm ◽  
B. V. Sletta
Keyword(s):  
Blood Donors ◽  
Tube System ◽  
Blood Samples ◽  
Pneumatic Tube ◽  
Pneumatic Tube System

scholarly journals Effect of Time and Haemolysis on Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT) Measurement on Blood Samples

2021 ◽  
Vol 11 (6-S) ◽  
pp. 114-122
Author(s):  
Tahia Jafar Abdo Alhakam Eshag ◽  
Maye M. Merghani ◽  
Nihad Elsadig Babiker
Keyword(s):  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Blood Clot ◽  
Activated Partial Thromboplastin Time ◽  
Medical Laboratory ◽  
Control Group ◽  
Case Group ◽  
Age And Gender ◽  
Blood Samples ◽  
And Gender

Background: Coagulation, also known as blood clotting, is the process by which blood convert from a liquid to a gel, forming a blood clot. It referred to haemostasis, the stopping of blood loss from a damaged vessel, followed by repair. Material and methods: This was cross sectional study conducted at the albawasla medical laboratory, Khartoum, Sudan during the period August to November, 2021 and to evaluate the effect of time and hemolysis on prothrombin time and activated partial thromboplastin time tests. 50 samples (case group) were collected from the patients attending police teaching hospital   and requested to the PT and APTT test in addition to that,50 apparently healthy donors with no history of any coagulation problems or any chronic disease were selected as control group. Three ml of venous blood samples were collected in container with Tri Sodium Citrate anticoagulant. The coagulation tests (PT and APTT) were performed using semiautomatic device (coagulometer machine MI). Results:  The result of this study revealed that; when compared the measurement of PT and APTT immediately and after one hour there was insignificant differences (p. v.>0.05).  also when compared the measurement of PT and APTT between hemolyzed and non-hemolyzed samples there was significant differences ( p. v.<0.05)  in addition when compared case and control for the PT and APTT immediately,  after one hour, hemolyzed and non-hemolyzed sample there was significant differences ( p. v.<0.05) except the APTT hemolyzed samples  and  insignificant differences with age and gender ( p. v.>0.05).  For the correlation there was significant correlation in the case group for the PT and APTT immediately, after one hour, and hemolyzed samples. Conclusion: In the cases group results showed insignificant differences in the results of PT and APTT between immediate sample and after 1 hour in and significant differences in the results of PT and APTT between hemolyzed and non-hemolyzed samples, also there was insignificant differences between age and gender, immediately, after one hr. and hemolyzed sample in PT and APTT. Keywords:  Homeostasis,  hemolyzed  sample, PT and APTT

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