BRINE SHRIMPS LETHALITY TEST OF ETHANOL EXTRACT AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF ETHYL ACETATE FRACTION OF BLIGHIA SAPIDA

2020 ◽  
pp. 208-212
Author(s):  
ADEKOLA MB ◽  
AREOLA JO ◽  
ORIYOMI OV ◽  
APATA JT ◽  
APALOWO OE ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Blighia Sapida

Objective: The objective of the study was to evaluate brine shrimp lethality of Blighia sapida stem-bark extract and its fractions and identify the bioactive constituents in the ethyl acetate fraction (EAF) using gas chromatography-mass spectrometry (GC-MS) technique. Methods: The ethanol extract (EE) and its fractions were subjected to lethality assay, and GC-MS analysis of EAF was carried out. Results: The lethality test showed a concentration-dependent mortality rate in the brine shrimp nauplii for the EE and its fractions. GC-MS analysis of EAF of the extract revealed the existence of 13 peaks of the GC-MS chromatogram with only one prominent compound, n-hexadecanoic acid (peak area of 10.13%). Conclusion: The result revealed the presence of 13 bioactive components in the EAF of the extract, the majority of which have been reported for different biological activities, hence, justifies the use of the plant in the treatment and management of different diseases ethnomedicinally.

scholarly journals GC-MS Analysis of Methanol and Ethyl Acetate Extract of fruits of Sphaeranthus indicus

2019 ◽  
Vol 9 (2) ◽  
pp. 28-30
Author(s):  
Kalpana Garg ◽  
Birendra Shrivastava ◽  
Anurag Bhargava
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Sphaeranthus Indicus

Introduction: To identify the various phytoconstituents present in the plant Sphaeranthus indicus by using gas chromatography and mass spectrometry. Meth­ods: The fruits of Sphaeranthus indicus were extracted with Different solvents of increasing polarity. The methanol and ethylacetate extract were subjected to GCMS analysis to detect the phytoconstituents. Results: Totally 26 compounds were identified. Among these 13 constituents in methanol extract and 13 constituents in ethylacetate extract were identified during the GC-MS analysis. Stigmasterol and lupeol which were identified in the plant is considered to have antiarthritic properties. Keywords: Sphaeranthus indicus, Gas chromatography, Mass spectrometry.

Identification of Secondary Metabolites from the Ethanol extract of the leaves of Bauhinia tomentosa by GC-MS Analysis

2021 ◽  
pp. 2335-2341
Author(s):  
R. Balabhaskar ◽  
K. Vijayalakshmi
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Secondary Metabolites ◽  
Mass Spectra ◽  
Ethanol Extract ◽  
Alpha Tocopherol ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Components ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

Gas Chromatography-Mass Spectrometry (GC-MS) is normally used for direct analysis of chemical components existing in herbal medicines. The medicinal plants are having numerous bioactive components which are identified even at less than 1ng by using GC-MS or LC-MS analysis. The aim of this study is to identify the secondary metabolites present in the leaves of B. tomentosa using gas chromatography-mass spectrometry (GC-MS) analysis. In the present study the ethanol extract of the leaves of Bauhinia tomentosa has been subjected to GC-MS analysis, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. GC-MS analysis revealed the presence of 14 secondary metabolites. These compounds were identified by comparing their retention times and peak areas with those from the literature and by interpretation of the mass spectra. The major secondary metabolites were DL-.alpha.-tocopherol (14.84%), 2-[(trimethylsilyl oxy]-, methyl ester, 1-alpha,2-alpha.-epoxy-1-beta-methylcholesta-4,6-dien-3-one (12.93%), pentacosenoic acid (12.71%), phytol (10.28%), Ethyl Isoallocholate (8.197%), Spirost-8-en-11-one-3-hydroxy-,(3-beta,5 alpha,14 beta,20 beta, 22 beta,25R)-(8.162%), Urs-12-en-28-ol (6.675%), 1-Octadecyne (5.702%) and Cholest-8-en-3-beta-ol,Acetate (5.426%). The compounds having area less that 5% were considered of no significance. These findings suggest that the presence of these secondary metabolites may be the cause for the properties exhibited by Bauhinia tomentosa. Thus, presence of various bioactive compounds justifies the use of the leaf for various ailments by traditional practitioners.

scholarly journals EVALUATION OF ANTIOXIDANT, ANTIBACTERIAL, AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF ETHYL ACETATE EXTRACT OF STREPTOMYCES OMIYAENSIS SCH2

2018 ◽  
Vol 11 (7) ◽  
pp. 271 ◽  
Author(s):  
Janpen Tangjitjaroenkun
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Antioxidant Power ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

Objective: The aim of this study was to evaluate the antibacterial and antioxidant properties of ethyl acetate extract of Streptomyces omiyaensis SCH2. The chemical constituents of the extract were investigated using gas chromatography-mass spectrometry (GC-MS).Methods: Secondary metabolites from S. omiyaensis were produced by submerge fermentation using ISP2 medium with 3% NaCl (w/v) for 21 days. The culture filtrate was extracted with ethyl acetate. The chemical constituents were detected in the GC-MS analysis. Antibacterial activity was performed using disc diffusion and broth microdilution methods. Antioxidant activity was evaluated by determining the reducing power capacity and free radical scavenging assays.Results: The GC-MS analysis of the SCH2 extract revealed the presence of four compounds. The main constituents were 2-phenylacetamide (79%). The extract exhibited the highest zone of inhibition against some pathogenic bacteria such as Enterobacter cloacae, Klebsiella pneumoniae, and Bacillus subtilis. In addition, the lowest minimum inhibitory concentration (IC) and minimum bactericidal concentration values of extract were obtained for E. cloacae (0.125 and 4 mg/ml). The extract showed antioxidant potential with IC50 values of 2.078.13±24.58 μg/ml and 475.74±4.56 μg/ml for 2,2-diphenyl-1-picryl-hydrazyl and 2,2′-azino-bis[3-ethylbenzothiazoline-6-sulphonic acid] assays, respectively. The ferric reducing antioxidant power value of extract was 110.36±1.75 mmol FeSO4.7H2O/g extract.Conclusion: This study indicated that S. omiyaensis extract possesses antibacterial and antioxidant activities. GC-MS analysis revealed the presence of major chemical constituents, acetamide, and pyrrolopyrazine which could be responsible for the biological activities. S. omiyaensis extract could be used as a potential of natural antibacterial and antioxidant agents for pharmaceutical and medical applications.

scholarly journals The Analysis of Bayer Liquor by SPME-GC-MS of Derivatized Organic Poisons

Proceedings ◽  
2021 ◽  
Vol 57 (1) ◽  
pp. 101
Author(s):  
Virgil Badescu ◽  
Raluca Senin
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Bayer Liquor ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Silylating Agent

The aim of this article was the gas chromatography–mass spectrometry (GC-MS) analysis oforganic matter from a residual liquor sample (S.C. Alum S.A., Tulcea), extracted by the solid-phasemicroextraction method (SPMA) and derivatized with N-(tert-butyldimethylsilyl)-Nmethyltrifluoroacetamide(MTBSTFA) as the silylating agent. [...]

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