scholarly journals Pola aktivitas enzim ligninolitik Pleurotus ostreatus pada limbah sludge pabrik kertas Activity pattern of ligninolytic enzyme of Pleurotus ostreatus in sludge waste of paper factory

2016 ◽  
Vol 76 (1) ◽  
Author(s):  
Happy WIDIASTUTI ◽  
. TRI-PANJI
Keyword(s):  
Rice Bran ◽  
Growth Phase ◽  
Fruiting Body ◽  
Activity Pattern ◽  
Vegetative Growth ◽  
Pleurotus Ostreatus ◽  
Ligninolytic Enzymes ◽  
Ligninolytic Enzyme ◽  
Oyster Mushroom ◽  
Sludge Waste

Summary Sludge is a solid waste abundantly available on paper factory that is economically unutilized and tends to pollute environment. This waste can be used as growth media for oyster mushroom (Pleurotus ostreatus) as edible mushroom and ligninolytic enzymes production as well. A research has been conducted to study the activity pattern of ligninolytic enzymes of oyster mushroom grown on the sludge waste of recycle paper factory. Six treatments were examinated consisted of three media combinations (sawdust, sludge, sludge mixed with sawdust), with and without supplementing with rice bran, lime, and gypsum, and two mushroom strains Bogor oyster mushroom (JTB) and China Taipei oyster mushroom (JTT). Monitoring of ligninolytic enzyme activity consisting of laccase, mangan peroxidase (Mn-P) and lignin peroxidase (Li-P),  was subsequently regularly started since inoculation, at vegetative phase (four and six weeks), primordial formation, phase of fruiting body formation, and two weeks after formation of fruiting body. Each treatment was repeated three times, so that 216 bag logs of oyster mushroom cultures were performed. The results showed that laccase, Mn-P, and Li-P activities could be observed on sludge or mixture of sludge+sawdust media inoculated with P. ostreatus. Generally, the highest activity of ligninolytic enzymes especially for laccase and MnP were observed at the first vegetative growth phase i.e. before emerging primordial of fruiting body (1.697 & 2.113 U/mL, 4.394 & 2.314 U/mL  respectively for JTB and JTT laccase and JTB & JTT Mn-P). The highest Li-P activity was affected by the kind of media and strain of inoculum. In sludge medium, the highest Li-P activity was observed in  vegetative growth phase (2.706 & 4.014 U/mL respectively for JTB and JTT) while in a mixture of sludge + sawdust the highest activity of that enzyme was observed in primordial phase of growth (2.509 & 1.9 U/mL respectively for JTB and JTT). Addition of supplement to the sludge increased ligninolytic activity, while laccase activity of sludge was suggested could be more enhanced by mixing the sludge with sawdust and enrich with rice bran, gypsum and lime. Ringkasan                                                Sludge merupakan limbah padat yang tersedia melimpah di pabrik kertas dan belum dimanfaatkan secara ekonomis sehingga berpotensi mencemari lingkungan. Limbah ini dapat dimanfaatkan sebagai medium tumbuh jamur konsumsi seperti jamur tiram (Pleurotus ostreatus) dan penghasil enzim ligninolitik. Penelitian dilakukan untuk mempelajari pola aktivitas enzim ligninolitik jamur tiram pada limbah sludge pabrik kertas selama fase vegetatif sampai setelah fase generatif. Enam perlakuan yang diuji berupa tiga kombinasi komposisi medium (serbuk gergaji, sludge, campuran sludge dan serbuk gergaji), dengan dan tanpa pengayaan, yaitu penambahan dedak, kapur, dan gipsum,  serta dua strain jamur tiram Bogor (JTB) dan jamur tiram China Taipei (JTT). Pengamatan aktivitas enzim ligninolitik meliputi lakase, mangan peroksidase (Mn-P) dan lignin peroksidase  (Li-P) dilakukan sejak saat inokulasi, pada fase vegetatif (empat dan enam minggu), pada saat pembentukan primordia, fase tubuh buah, dan dua minggu setelah pembentukan tubuh buah. Masing-masing perlakuan diulang tiga kali sehingga terdapat 216 bag log jamur tiram. Hasil penelitian menunjukkan bahwa aktivitas ligninolitik dijumpai pada medium sludge dan campuran sludge+serbuk gergaji yang diino-kulasi P. ostreatus. Aktivitas enzim ligninolitik tertinggi khususnya lakase dan MnP teramati pada fase pertumbuhan vegetatif pertama yaitu sebelum terbentuknya primordia (1,697 & 2,113 U/mL, 4,394 & 2,314 U/mL  masing-masing untuk lakase JTB dan JTT dan MnP  JTB & JTT). Aktivitas LiP tertinggi dipengaruhi oleh jenis medium dan strain inokulum. Pada medium sludge, aktivitas LiP tertinggi dijumpai pada fase vegetatif (2,706 & 4,014 U/ml masing-masing untuk JTB dan JTT) sedangkan pada medium campuran sludge+serbuk gergaji, aktivitas enzim  ter-tinggi dijumpai  pada fase primordia (2,509 & 1,9 U/ml berturut-turut untuk JTB dan JTT). Pengayaan sludge meningkatkan aktivitas ligninolitik, sedangkan aktivitas lakase pada sludge diduga dapat lebih ditingkatkan dengan menambahkan serbuk gergaji disertai pengayaan berupa gipsum, dedak, dan kapur.

scholarly journals Pola aktivitas enzim ligninolitik Pleurotus ostreatus pada limbah sludge pabrik kertas Activity pattern of ligninolytic enzyme of Pleurotus ostreatus in sludge waste of paper factory

2016 ◽  
Vol 76 (1) ◽  
Author(s):  
Happy WIDIASTUTI ◽  
. TRI-PANJI
Keyword(s):  
Rice Bran ◽  
Growth Phase ◽  
Fruiting Body ◽  
Activity Pattern ◽  
Vegetative Growth ◽  
Pleurotus Ostreatus ◽  
Ligninolytic Enzymes ◽  
Ligninolytic Enzyme ◽  
Oyster Mushroom ◽  
Sludge Waste

Summary Sludge is a solid waste abundantly available on paper factory that is economically unutilized and tends to pollute environment. This waste can be used as growth media for oyster mushroom (Pleurotus ostreatus) as edible mushroom and ligninolytic enzymes production as well. A research has been conducted to study the activity pattern of ligninolytic enzymes of oyster mushroom grown on the sludge waste of recycle paper factory. Six treatments were examinated consisted of three media combinations (sawdust, sludge, sludge mixed with sawdust), with and without supplementing with rice bran, lime, and gypsum, and two mushroom strains Bogor oyster mushroom (JTB) and China Taipei oyster mushroom (JTT). Monitoring of ligninolytic enzyme activity consisting of laccase, mangan peroxidase (Mn-P) and lignin peroxidase (Li-P),  was subsequently regularly started since inoculation, at vegetative phase (four and six weeks), primordial formation, phase of fruiting body formation, and two weeks after formation of fruiting body. Each treatment was repeated three times, so that 216 bag logs of oyster mushroom cultures were performed. The results showed that laccase, Mn-P, and Li-P activities could be observed on sludge or mixture of sludge+sawdust media inoculated with P. ostreatus. Generally, the highest activity of ligninolytic enzymes especially for laccase and MnP were observed at the first vegetative growth phase i.e. before emerging primordial of fruiting body (1.697 & 2.113 U/mL, 4.394 & 2.314 U/mL  respectively for JTB and JTT laccase and JTB & JTT Mn-P). The highest Li-P activity was affected by the kind of media and strain of inoculum. In sludge medium, the highest Li-P activity was observed in  vegetative growth phase (2.706 & 4.014 U/mL respectively for JTB and JTT) while in a mixture of sludge + sawdust the highest activity of that enzyme was observed in primordial phase of growth (2.509 & 1.9 U/mL respectively for JTB and JTT). Addition of supplement to the sludge increased ligninolytic activity, while laccase activity of sludge was suggested could be more enhanced by mixing the sludge with sawdust and enrich with rice bran, gypsum and lime. Ringkasan                                                Sludge merupakan limbah padat yang tersedia melimpah di pabrik kertas dan belum dimanfaatkan secara ekonomis sehingga berpotensi mencemari lingkungan. Limbah ini dapat dimanfaatkan sebagai medium tumbuh jamur konsumsi seperti jamur tiram (Pleurotus ostreatus) dan penghasil enzim ligninolitik. Penelitian dilakukan untuk mempelajari pola aktivitas enzim ligninolitik jamur tiram pada limbah sludge pabrik kertas selama fase vegetatif sampai setelah fase generatif. Enam perlakuan yang diuji berupa tiga kombinasi komposisi medium (serbuk gergaji, sludge, campuran sludge dan serbuk gergaji), dengan dan tanpa pengayaan, yaitu penambahan dedak, kapur, dan gipsum,  serta dua strain jamur tiram Bogor (JTB) dan jamur tiram China Taipei (JTT). Pengamatan aktivitas enzim ligninolitik meliputi lakase, mangan peroksidase (Mn-P) dan lignin peroksidase  (Li-P) dilakukan sejak saat inokulasi, pada fase vegetatif (empat dan enam minggu), pada saat pembentukan primordia, fase tubuh buah, dan dua minggu setelah pembentukan tubuh buah. Masing-masing perlakuan diulang tiga kali sehingga terdapat 216 bag log jamur tiram. Hasil penelitian menunjukkan bahwa aktivitas ligninolitik dijumpai pada medium sludge dan campuran sludge+serbuk gergaji yang diino-kulasi P. ostreatus. Aktivitas enzim ligninolitik tertinggi khususnya lakase dan MnP teramati pada fase pertumbuhan vegetatif pertama yaitu sebelum terbentuknya primordia (1,697 & 2,113 U/mL, 4,394 & 2,314 U/mL  masing-masing untuk lakase JTB dan JTT dan MnP  JTB & JTT). Aktivitas LiP tertinggi dipengaruhi oleh jenis medium dan strain inokulum. Pada medium sludge, aktivitas LiP tertinggi dijumpai pada fase vegetatif (2,706 & 4,014 U/ml masing-masing untuk JTB dan JTT) sedangkan pada medium campuran sludge+serbuk gergaji, aktivitas enzim  ter-tinggi dijumpai  pada fase primordia (2,509 & 1,9 U/ml berturut-turut untuk JTB dan JTT). Pengayaan sludge meningkatkan aktivitas ligninolitik, sedangkan aktivitas lakase pada sludge diduga dapat lebih ditingkatkan dengan menambahkan serbuk gergaji disertai pengayaan berupa gipsum, dedak, dan kapur.

scholarly journals KAJIAN PENGGUNAAN SERASAH DAUN KAKAO UNTUK SUBTITUSI SERBUK GERGAJI DAN DEDAK PADI SEBAGAI MEDIA TANAM JAMUR TIRAM PUTIH (Pleurotus ostreatus)

KOVALEN ◽  
2017 ◽  
Vol 3 (1) ◽  
pp. 41
Author(s):  
Abd. Rahman Razak ◽  
Susanti Susanti ◽  
Nurhaeni Nurhaeni ◽  
Muh. Alwi
Keyword(s):  
Leaf Litter ◽  
Rice Bran ◽  
Pleurotus Ostreatus ◽  
Oyster Mushroom ◽  
Fruiting Bodies ◽  
Treatment Comparison ◽  
Treatment Standard ◽  
Nutrient Value ◽  
Growing Medium ◽  
Oyster Mushrooms

Has done research on the study of the use of cocoa leaf litter for substitution sawdust and rice bran as a growing medium white oyster mushroom (Pleurotus ostreatus). This study aims to determine the ability of the cocoa leaf litter in substitute sawdust and rice bran as oyster mushroom media and to determine the nutrient value of the conversion of media into fruiting bodies of oyster mushrooms. Treatment comparison of leaf litter cocoa (SDK), sawdust (SG) and rice bran (DP) there are seven various ie first treatment / standard (SDK: 0 kg, SG: 5 kg, DP: 3 kg), second (SDK: 1 , 5 kg, SG: 5 kg, DP: 1.5 kg), third (SDK: 3 kg, SG: 5 kg, DP: 0 kg), fourth (SDK: 2.5 kg, SG: 2.5 kg DP: 3 kg), fifth (SDK: 5 kg, SG: 3 kg, DP: 0 kg), sixth (SDK: 5 kg, SG: 1.5 kg, DP: 1.5 kg), and seventh (SDK: 5 kg, SG: 0 kg, DP: 3 kg). The results showed that both treatments are best treated with a total weight of 181.122 g fruit.Keywords: cocoa leaf litter, white oyster mushroom

scholarly journals Aktivitas enzim ligninolitik Pleurotus ostreatus pada media yang mengandung TKKS dan aplikasinya untuk dekolorisasi zat warna (Activity of ligninolytic enzyme of Pleurotus ostreatus on media containing OPEFB and their application for dyes decolorization)

2019 ◽  
Vol 87 (1) ◽  
Author(s):  
Firda DIMAWARNITA ◽  
TRI - PANJI
Keyword(s):  
Enzyme Activity ◽  
Lignin Peroxidase ◽  
Congo Red ◽  
Pleurotus Ostreatus ◽  
Ligninolytic Enzymes ◽  
Ligninolytic Enzyme ◽  
White Rot ◽  
Enzyme Extract ◽  
Media Composition ◽  
Dyes Decolorization

Ligninolytic enzymes are known as extracellular enzymes produced by the white rot fungi class of basidiomycetes. One of the most well-known fungi of the white rot fungus isPleurotus ostreatus. The aim of this study to calculate the activity of ligninolytic enzymes in the growth media of Pleurotus ostreatusand their application in decolorization of dye colour. The ligninolytic enzyme extract obtained was used to decolorize bluedyes (MethyleneBlue)and red dyes(Congo Red). The highest laccase enzyme activity was in the first month of 0.35 U/mL with E1 media composition; the highest manganese peroxidase (MnP) enzyme activity was in the fourth month at 31.818 U / mL with E4 media composition; and the highest lignin peroxidase (LiP) enzyme activity was in the fifth month at 0.269 U / mL with E1 media composition. The enzyme extract obtained was then applied to decolorize red and blue dyes. Decolorization of dyes was measured using spectrophotometry with a blue wavelength of 470 nm and red 685 nm. The highest reduction in decolorization of blue dye and red dye was 12 hours with concentration of enzyme addition of 0.5%. Based on these results, ligninolytic enzymes potentiallyto be developed as bioactive agents for detergents.[Keywords: decolorization, laccase, mangan peroxidase, lignin peroxidase, spectrofotometry] AbstrakEnzim ligninolitik dikenal sebagai enzim ekstraseluler yang dihasilkan oleh jamur pelapuk putih golongan basidiomycetes. Salah satu jamur dari golongan jamur pelapuk putih yang banyak dikenal adalah Pleurotus ostreatus. Penelitian ini bertujuan menghitung aktivitas enzim ligninolitik pada media pertumbuhan jamur tiram  (Pleurotus ostreatus) dan aplikasinya dalam dekolorisasi zat warna.  Ekstrak enzim ligninolitik yang didapatkan kemudian dimanfaatkan untuk dekolorisasi zat warna biru(Methylene Blue)dan merah (Congo Red). Aktivitas enzim lakase tertinggi ada pada bulan pertama sebesar 0,35 U/mL dengan komposisi media E1; aktivitas enzim mangan peroksidase (MnP) tertinggi ada pada bulan keempat sebesar 31,818 U/mL dengan komposisi media E4; dan aktivitas enzim lignin peroksidase (LiP) tertinggi ada pada bulan kelima sebesar 0,269 U/mL dengan komposisi media E1. Ekstrak enzim yang didapat kemudian diaplikasikan untuk dekolorisasi zat warna merah dan biru. Dekolorisasi zat warna diukur menggunakan spektrofotometri dengan panjang gelombang biru pada 470 nm dan merah pada 685 nm. Penurunan dekolorisasi zat warna birudan zat warna merahtertinggi selama 12jam dengan konsentraasi penambahan enzim sebesar 0,5%.Berdasarkan hasil tersebut, enzim ligninolitik sangat potensial untuk dikembangkan sebagai agen bioaktif untuk deterjen.[Kata kunci: dekolorisasi, lakase, mangan peroksidase, lignin peroksidase,  spektrofotometri]

The tripartite relationship in gill-knot disease of the oyster mushroom, Pleurotus ostreatus (Jacq.: Fr.) Kummer

1996 ◽  
Vol 74 (8) ◽  
pp. 1402-1408 ◽  
Author(s):  
Kaku Tsuda ◽  
Kazuyoshi Futai ◽  
Hajime Kosaka
Keyword(s):  
Adult Female ◽  
Adult Male ◽  
Fruiting Body ◽  
Pleurotus Ostreatus ◽  
Laboratory Experiments ◽  
Oyster Mushroom ◽  
Fruiting Bodies ◽  
Fungus Gnat ◽  
Western Japan ◽  
Tripartite Relationship

A disease that causes knots on the gills of the oyster mushroom, Pleurotus ostreatus, has been reported from western Japan. Nematodes inhabit and lay many eggs inside the gill knots. It is surprising that the nematodes causing this disease live in the fruiting body of this fungus, which is known to be nematophagous. In the present study, the fungus gnat Rhymosia domestica (Mycetophilidae, Diptera) was confirmed to be the vector of the nematodes inside the gill knots. This nematode has different adult stages, i.e., a mycetophagous adult female, an adult female infective to the fungus gnat, an adult male, and a mature entomophagous adult female. The occurrence of gill-knot disease was investigated in the field. Laboratory experiments determined that the fungus gnat could not itself form gill knots, because formation of knots was not observed on the gills of fruiting bodies incubated with nematode-free gnats. Observation by means of microscopy and the spore patterns of diseased fruiting bodies confirmed that the knots consist of dedifferentiated hyphal tissues, which do not form spores.

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