scholarly journals Aktivitas Antiinflamasi Crude Extract Fukoidan dari Sargassum crassifolium pada Sel RAW 264.7 yang Diinduksi LPS

2019 ◽  
pp. 97-105
Author(s):  
Pangartika Hikariastri ◽  
Hendig Winarno ◽  
Kusmardi Kusmardi ◽  
Dian Ratih Laksmitawati ◽  
Syamsudin Abdillah
Keyword(s):  
Crude Extract ◽  
Macrophage Activation ◽  
Basic Mechanism ◽  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Proinflammatory Mediators ◽  
Promising Target ◽  
Tnf Α ◽  
Anti Inflammatory

Inflammation is known as the basic mechanism underlying various chronic diseases. Macrophage activation by inflammatory stimulus induces the release of inflammatory mediators, thus the mediators becoming a promising target of anti-inflammatory drug development. Previous studies indicated that fucoidan has anti-inflammatory activity by inhibiting the release of proinflammatory mediators. The aim of this study is determining anti-inflammatory activity of fucoidan crude extract form Sargassum crassifolium Garut waters by observing its effect on proinflammatory cytokines TNF-α, IL-1β, dan IL-6. Fucoidan is a polysaccharide substance which has various characteristics, depending on the source and the extraction method which is influencing its bioactivity. Sargassum crassifolium collected from Garut-West Java is extracted using diluted HCl 0,1 M and precipitated with ethanol to obtain fucoidan crude extract. The crude extract is tested on LPS-induced RAW 264.7 cells to evaluate its effect on TNF-α, IL-1β, and IL-6 level using ELISA method. The result showed that fucoidan crude extract decreased the level of TNF-α by the dose of 25 and 50 μg/ml, and decreased the level of IL-1β and IL-6 by the dose of 25 μg/ml. The dose of 50 μg/ml failed to inhibit IL-1β and IL-6 production. This study showed that fucoidan crude extract derived from S. crassifolium has anti-inflammatory activity to RAW 264.7 cells by the dose of 25 μg/ml.

scholarly journals Anti-Inflammatory Activity of Four Triterpenoids Isolated from Poriae Cutis

Foods ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3155
Author(s):  
Lijia Zhang ◽  
Mengzhou Yin ◽  
Xi Feng ◽  
Salam A. Ibrahim ◽  
Ying Liu ◽  
...  
Keyword(s):  
High Speed ◽  
High Performance ◽  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Dependent Manner ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Dose Dependent

In this study, triterpenoid compounds from Poriae Cutis were separated by high-speed countercurrent chromatography (HSCCC) and identified using ultra-high performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS/MS) and nuclear magnetic resonance (NMR). The in vitro anti-inflammatory activities of the purified triterpenoids on RAW 264.7 cells were also investigated. Triterpenoids, poricoic acid B, poricoic acid A, dehydrotrametenolic acid, and dehydroeburicoic acid were obtained; their levels of purity were 90%, 92%, 93%, and 96%, respectively. The results indicated that poricoic acid B had higher anti-inflammatory activity than those of poricoic acid A by inhibiting the generation of NO in lipopolysaccharide (LPS)-induced RAW 264.7 cells. However, dehydrotrametenolic acid and dehydroeburicoic acid had no anti-inflammatory activity. In addition, the production of cytokines (TNF-α, IL-1β, and IL-6) in cells treated with poricoic acid B decreased in a dose-dependent manner in the concentration range from 10 to 40 μg/mL. The results provide evidence for the use of Poriae Cutis as a natural anti-inflammatory agent in medicines and functional foods.

scholarly journals Cytotoxicity and Anti-Inflammatory Activity of Tamarillo (Solanum betaceum Cav.) Peel Extract in Lipopolysaccharide Stimulated RAW 264.7 Cells

e-GIGI ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Novelya Li ◽  
Wilvia Li
Keyword(s):  
Treated Group ◽  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Gastric Bleeding ◽  
Traditional Medicines ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Peel Extract ◽  
Solanum Betaceum

Abstrak: Obat anti inflamasi sering diresepkan dalam bidang kedokteran gigi. Umumnya obat-obat ini memiliki efek samping beragam, dari yang ringan hingga parah seperti perdarahan lambung. Oleh karena itu, bahan anti inflamasi yang alami dan lebih aman diperlukan sebagai alternatif. Solanum betaceum Cav., dikenal sebagai terong Belanda, merupakan buah eksotik yang dapat juga dipergunakan sebagai bahan obat. Limbah buah seperti kulit dan biji biasanya tidak dikonsumsi. Beberapa penelitian menunjukkan bahwa limbah dari berbagai jenis buah mengandung senyawa bioaktif yang bermanfaat. Penelitian ini bertujuan untuk mengetahui sitotoksistas dan kemampuan anti-inflamasi dari ekstrak kulit terong Belanda terhadap makrofag lini RAW 264.7 yang diinduksi oleh lipopolisakarida (LPS). Sitotoksistas diuji untuk menentukan konsentrasi yang aman dengan menggunakan metode MTS. Aktivitas anti-inflamasi dinilai dengan membandingkan kadar PGE-2, TNF-α, dan IL-1β pada sel RAW 264.7 yang distimulasi LPS antara kelompok yang diberikan ekstrak dan yang tidak diberikan ekstrak menggunakan metode ELISA. Hasil penelitian mendapatkan bahwa ekstrak kulit terong Belanda dapat menekan produksi PGE-2, TNF-α, dan IL-1β pada sel RAW 264.7 yang diinduksi dengan LPS. Simpulan penelitian ini ialah ekstrak kulit Solanum betaceum Cav memiliki kemampuan antiinflamasi melalui penekanan produksi PGE-2, TNF-α, dan IL-1β pada sel RAW 264.7 yang diinduksi LPS.Kata kunci: ekstrak kulit Solanum betaceum Cav. (terong Belanda), sitotoksistas, antiinflamasi  Abstract: Anti-inflammatory agents are commonly prescribed in dentistry. Most of them have side effects varying from mild to severe such as gastric bleeding. Therefore, a safer and natural anti-inflammatory agent is needed as an alternative. Solanum betaceum Cav., known as tamarillo, is one of exotic fruits used in traditional medicines. The fruit wastes such as peels and seeds, are usually unconsumed. However, many investigators have reported that wastes from numerous fruits had potent bioactive compounds. This study was aimed to assess the cytotoxicity and anti-inflammatory activities of tamarillo peel extract (TPE) in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophage cell line. The cytotoxicity of TPE was performed to determine the non-toxic concentration by using MTS method. The inflammatory markers measured in this study were PGE-2, TNF-α, and IL-1β. Their concentrations were measured by using ELISA based assay. The anti-inflammatory activity was determined by comparing the reduction of the inflammatory mediators between the LPS stimulated RAW 264.7 cells treated with TPE and the non-treated group. This study revealed that TPE could reduce the production of PGE-2, TNF-α, and IL-1β in LPS stimulated RAW 264.7 cells. In conclusion, tamarillo peel extract possess an anti-inflammatory effect by reducing the production of PGE-2, TNF-α, and IL-1β in LPS stimulated RAW 264.7 cells.Keywords: Solanum betaceum Cav. (tamarillo) peel extract, cytotoxicity, anti-inflammatory

scholarly journals A New Flavonol From Saussurea involucrata With Anti-Inflammatory Activity

2021 ◽  
Vol 16 (5) ◽  
pp. 1934578X2110076
Author(s):  
Sheng Pan ◽  
Zi-Guan Zhu
Keyword(s):  
Nitric Oxide ◽  
Aerial Part ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Spectroscopic Methods ◽  
Soluble Extract ◽  
Saussurea Involucrata ◽  
Tnf Α ◽  
Anti Inflammatory

A new flavonol named 6-(2'',3''-epoxy-3''-methylbutyl)-resokaempferol (1), together with five known compounds (2-6) were isolated from the EtOAc-soluble extract of the aerial part of Saussurea involucrata. Their structures were elucidated on the basis of spectroscopic methods. All compounds were evaluated for their anti-inflammatory effects by measuring the production of nitric oxide (NO) and TNF-α in vitro. Among them, compound 1 showed potential inhibitory activity on the production of NO and TNF-α in LPS-induced RAW 264.7 cells with IC50 values of 48.0 ± 1.5 and 41.4 ± 1.7 µM, respectively.

scholarly journals Fisetin inhibits lipopolysaccharide-induced inflammatory response by activating β-catenin, leading to a decrease in endotoxic shock

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ilandarage Menu Neelaka Molagoda ◽  
Jayasingha Arachchige Chathuranga C Jayasingha ◽  
Yung Hyun Choi ◽  
Rajapaksha Gedara Prasad Tharanga Jayasooriya ◽  
Chang-Hee Kang ◽  
...  
Keyword(s):  
Glycogen Synthase ◽  
Endotoxic Shock ◽  
Inflammatory Activity ◽  
Prostaglandin E ◽  
Necrosis Factor Alpha ◽  
Zebrafish Larvae ◽  
Proinflammatory Mediators ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Gsk 3Β

AbstractFisetin is a naturally occurring flavonoid that possesses several pharmacological benefits including anti-inflammatory activity. However, its precise anti-inflammatory mechanism is not clear. In the present study, we found that fisetin significantly inhibited the expression of proinflammatory mediators, such as nitric oxide (NO) and prostaglandin E2 (PGE2), and cytokines, such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Additionally, fisetin attenuated LPS-induced mortality and abnormalities in zebrafish larvae and normalized the heart rate. Fisetin decreased the recruitment of macrophages and neutrophils to the LPS-microinjected inflammatory site in zebrafish larvae, concomitant with a significant downregulation of proinflammatory genes, such as inducible NO synthase (iNOS), cyclooxygenase-2a (COX-2a), IL-6, and TNF-α. Fisetin inhibited the nuclear localization of nuclear factor-kappa B (NF-κB), which reduced the expression of pro-inflammatory genes. Further, fisetin inactivated glycogen synthase kinase 3β (GSK-3β) via phosphorylation at Ser9, and inhibited the degradation of β-catenin, which consequently promoted the localization of β-catenin into the nucleus. The pharmacological inhibition of β-catenin with FH535 reversed the fisetin-induced anti-inflammatory activity and restored NF-κB activity, which indicated that fisetin-mediated activation of β-catenin results in the inhibition of LPS-induced NF-κB activity. In LPS-microinjected zebrafish larvae, FH535 promoted the migration of macrophages to the yolk sac and decreased resident neutrophil counts in the posterior blood island and induced high expression of iNOS and COX-2a, which was accompanied by the inhibition of fisetin-induced anti-inflammatory activity. Altogether, the current study confirmed that the dietary flavonoid, fisetin, inhibited LPS-induced inflammation and endotoxic shock through crosstalk between GSK-3β/β-catenin and the NF-κB signaling pathways.

Synthesis and anti-inflammatory activity of novel steroidal chalcones with 3β-pregnenolone ester derivatives in RAW 264.7 cells in vitro

Steroids ◽  
2021 ◽  
pp. 108830
Author(s):  
Xiaorui Cai ◽  
Fei Sha ◽  
Chuanyi Zhao ◽  
Zhiwei Zheng ◽  
Shulin Zhao ◽  
...  
Keyword(s):  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Anti Inflammatory

RETRACTED: Anti-inflammatory Activity of 4-Arylcoumarins from EndophyticStreptomyces aureofaciensCMUAc130 in Murine Macrophage RAW 264.7 Cells

2007 ◽  
Vol 36 (2) ◽  
pp. 203-211 ◽  
Author(s):  
Thongchai Taechowisan ◽  
Pittaya Tuntiwachwuttikul ◽  
Chunhua Lu ◽  
Yuemao Shen ◽  
Saisamorn Lumyong ◽  
...  
Keyword(s):  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Murine Macrophage ◽  
Anti Inflammatory

scholarly journals IN VITRO ANTI-INFLAMMATORY ACTIVITY OF THE COMPONENTS OF AMOMUM TSAO-KO IN MURINE MACROPHAGE RAW 264.7 CELLS

2018 ◽  
Vol 15 (2) ◽  
pp. 26-34
Author(s):  
Chun Whan Choi ◽  
Ju Young Shin ◽  
Changon Seo ◽  
Seong Su Hong ◽  
Eun-Kyung Ahn ◽  
...  
Keyword(s):  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Murine Macrophage ◽  
Anti Inflammatory

scholarly journals Anti-Inflammatory Activity of Geldanamycin and Its Derivatives in LPS-Induced RAW 264.7 Cells

2019 ◽  
Vol 09 (04) ◽  
pp. 398-421 ◽  
Author(s):  
Thongchai Taechowisan ◽  
Winyou Puckdee ◽  
Watcharee Waratchareeyakul ◽  
Waya S. Phutdhawong
Keyword(s):  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Anti Inflammatory

scholarly journals Huangkui Capsule Attenuates Lipopolysaccharide-Induced Acute Lung Injury and Macrophage Activation by Suppressing Inflammation and Oxidative Stress in Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Jinfang Deng ◽  
Zhenpeng He ◽  
Xiuru Li ◽  
Wei Chen ◽  
Ziwen Yu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Acute Lung Injury ◽  
Lung Injury ◽  
Macrophage Activation ◽  
Neutrophil Infiltration ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Myeloperoxidase Activity ◽  
Tnf Α ◽  
And Oxidative Stress

Background. Huangkui capsule (HKC) comprises the total flavonoid extract of flowers of Abelmoschus manihot (L.) Medicus. This study aimed to explore the effects of HKC on lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice and LPS-stimulated RAW 264.7 cells. Methods. Enzyme-linked immunosorbent assay, histopathology, spectrophotometry, and quantitative real-time polymerase chain reaction were used for the assessments. Statistical analysis was performed using a one-way analysis of variance. Results. LPS significantly increased lung inflammation, neutrophil infiltration, and oxidative stress and downregulated lung miR-451 expression. Treatment with HKC dramatically attenuated the lung wet/dry weight ratio, reduced the total cell count in the bronchoalveolar lavage fluid (BALF), and inhibited myeloperoxidase activity in the lung tissues 24 h after LPS challenge. Histopathological analysis demonstrated that HKC attenuated LPS-induced tissue oedema and neutrophil infiltration in the lung tissues. Additionally, the concentrations of tumour necrosis factor- (TNF-) α and interleukin- (IL-) 6 in BALF and IL-6 in the plasma reduced after HKC administration. Moreover, HKC could enhance glutathione peroxidase and catalase activities and upregulate the expression of miR-451 in the lung tissues. In vitro experiments revealed that HKC inhibited the production of nitric oxide, TNF-α, and IL-6 in LPS-induced RAW 264.7 cells and mouse primary peritoneal macrophages. Additionally, HKC downregulated LPS-induced transcription of TNF-α and IL-6 in RAW 264.7 cells. Conclusions. These findings suggest that HKC has anti-inflammatory and antioxidative effects that may protect mice against LPS-induced ALI and macrophage activation.

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