scholarly journals Isolation and characterization of lignocellulolytic microbes from oil palm empty fruit bunches (EFB)

Author(s):  
Ariana Ariana ◽  
Krishna P. Candra

Oil palm empty fruit bunches (EFB) is one of the major by-products of palm oil production. This lignocellulosic biomass is commonly used as a fertilizer at oil palm plantations. Unfortunately, the composting process of EFB is very slow. This study aimed to identify potential lignocellulosic microbes isolated from EFB. This information is essential for improving EFB biodegradation process by reducing the decomposition time. Samples of approximately 6, 12, and 24-month-old EFB were obtained from two palm oil mills in East Kalimantan, Indonesia. The isolation of lignocellulytic microbes utilized selective medium cellulose congo red agar (CCRA) while its characterization used lignin agar (LgA) and oil palm empty fruit bunches agar (EFBCRA). As much as 430 isolates were successfully collected and 12 of them exhibited promising capability to synthesize lignocellulolytic enzyme, the key for FEB degradation.

Konversi ◽  
2018 ◽  
Vol 6 (2) ◽  
pp. 9
Author(s):  
Ramli Ramli ◽  
Marlinda Marlinda

Abstract- East Kalimantan, especially Kutai regency produces 1,112,442 tons/year of palm oil. Oil palm empty fruit bunches (EFB) are one of the wastes generated in the processing of palm oil that is equal to 20-23% of the fresh fruit bunches, so the amount of EFB that can be generated is 244,737.24 tons/year. The purpose of this study is to determine the effect of palm sugar as a nutrient to nutrient nitrogen EM4, phosphor and potassium in composting EFB. This research was conducted by varying the mass of Palm Sugar: 0.4000 g, 0.6000 g, 0.8000 g, 1.0000 g and 1.2000 g. The main composting process that was carried out by adding EM4 solution of 10 mL and 100 mL sugar solution into 200 grams EFB, letting it stand for 30 days, and after that the analysis was conducted. The total content of nutrients is highest on the mass of 1.2000 g palm sugar that is 3.174%. The conclusion of this study is the greater the mass of Palm Sugar is added, the greater the concentration of nutrients found in the composting by using bacterial EM4. Keywords: bio-activator EM4, palm Sugar, EFB.


Konversi ◽  
2015 ◽  
Vol 4 (2) ◽  
pp. 12
Author(s):  
Marinda Rahim ◽  
Mardhiyah Nadir

Abstrak- Sebagai daerah sentra pengembangan perkebunan kelapa sawit dan industri crude palm oil (CPO), Kalimantan Timur memiliki potensi untuk menghasilkan tandan kosong kelapa sawit (TKKS) dalam jumlah yang cukup besar. TKKS merupakan limbah padat dari hasil industri CPO yang menggunakan tandan buah sawit (TBS) sebagai bahan bakunya.  Bagian TKKS adalah 23% dari TBS. TKKS memiliki nilai ekonomi yang tinggi jika diolah lebih lanjut. Salah satu cara yang digunakan adalah dengan menghidrolisis kandungan pentosan di dalam TKKS menjadi furfural. Tujuan penelitian ini adalah megembangkan teknik hidrolisis satu tahap TKKS menjadi furfural dengan bantuan gelombang mikro untuk mendapatkan waktu optimum yang dapat mengasilkan furfural maksimum.. Pada penelitian ini 10 gram TKKS ditambahkan dengan 250 mL H2SO4 15% sebagai katalis. Campuran kemudian dihidrolisis menggunakan bantuan gelombang mikro dengan variasi waktu 15, 30, 45, 60, 75, 90, dan 105 menit. Hasil analisa dengan Gas Chromatography (GC) menunjukkan hasil furfural tertinggi diperoleh pada waktu 75 menit dengan konsentrasi 1,34 mg/mL.  Kata Kunci: furfural, gelombang mikro, hidrolisis, TKKS Abstract- As a regional center for the development of oil palm plantations and crude palm oil (CPO) industry, East Kalimantan has the potential to produce oil palm empty fruit bunches (EFB) in large enough quantities. EFB is the solid waste from the palm oil industry which uses palm fruit bunches (FFB) as a raw material. EFB part is 23% of FFB. Whereas EFB has a high economic value if processed further. One of the method that is used to hydrolyze the content of pentosan in EFB into furfural. The purpose of this research is to develop one step hydrolysis technique of EFB into furfural  with microwaves assistance  to obtain the optimum time which can produce maximum furfural. In this research 10 grams of EFB was added with 250 mL of H2SO4 15%  as the catalyst. The mixture was then hydrolyzed using microwave-assisted with a time variety of for 15, 30, 45, 60, 75, 90, and105 minutes. The results of analysis by Gas Chromatography (GC) showed that the highest results of furfural  was at 75 minutes with a concentration of 1.34 mg / mL. Keywords: EFB, furfural, microwaves, hydrolysis


2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Tracy Miller ◽  
Diana Elizabeth Waturangi ◽  
Tresnawati Purwadaria

Abstract Objective This research was aimed to isolate cellulolytic molds in empty fruit bunches of oil palm (EFBOP) and soils from palm oil plantation area and identify their enzyme activities to digest EFBOP. Results A total of seven molds were successfully isolated and screened for their enzyme activities from EFBOP and the soils. The enzymes from each isolate were produced in submerged culture using Mineral Mandels and 3% of alkali pretreated pollard in triplicates. The results indicated that all of the isolates were able to hydrolyze Carboxymethyl Cellulose (CMC), Whatmann No. 1 filter paper, and also EFBOP to sugars with reducing ends that reacted to 3,5-Dinitrosalicylic acid (DNS). The CMCase activity of isolate X showed the highest while the lowest was found for isolate MT8. Filter paperase (FPase) activity of isolate X performed the highest wile the lowest were found from isolate MT3 and MT6. The saccharification activity of isolate P showed the highest while MT6 performed the lowest activity


2017 ◽  
Vol 25 (3) ◽  
pp. 161-170
Author(s):  
Henny Lydiasari ◽  
Ari Yusman Manalu ◽  
Rahmi Karolina

The potency of oil palm empty fruit bunches (OPEFB) fibers as one of the by-products of processing oil palm is increasing significantly so that proper management is needed in reducing environmental impact. One of the utilization of OPEFB fibers is as a substitution material in construction which usually the material is derived from non-renewable mining materials so that the number is increasingly limited. Therefore, it is necessary to study to know the performance of OPEFB fiber in making construction products especially concrete. In this case, the experiment was conducted using experimental method with variation of fiber addition by 0%, 10%, 15%, 20%, 25%, and 30%. Each specimen was tested by weight, slump value, compressive strength, tensile strength, elasticity and crack length. As the results, the variation of fibers addition by 10%, decrease of slump value is 7%, concrete weight is 3% and crack length is 8% while increase of the compressive strength is 2.7% and the modulus of elasticity is 33.3% but its tensile strength decreased insignificantly by 0.05% . Furthermore, the addition of fibers above 10% to 30% decreased compressive strength is still below 10% and tensile strength below 2% while the weight of concrete, slump value and crack length decreased. Therefore, the addition of 10% can replace the performance of concrete without fiber but the addition of above 10% can still be used on non-structural concrete.


2013 ◽  
Vol 93 (2) ◽  
pp. 628-634 ◽  
Author(s):  
M.K. Mohamad Haafiz ◽  
S.J. Eichhorn ◽  
Azman Hassan ◽  
M. Jawaid

2013 ◽  
Vol 33 (2) ◽  
pp. 295-303 ◽  
Author(s):  
Cintia Anabela Mazzucotelli ◽  
Alejandra Graciela Ponce ◽  
Catalina Elena Kotlar ◽  
María del Rosario Moreira

2019 ◽  
Vol 48 (3) ◽  
pp. 190-197 ◽  
Author(s):  
HJW Mutsaers

Palm oil is a valuable product used all over the world in a vast number of products for daily use. The oil palm, once well established, is very productive, captures large amounts of carbon and provides good protection to the soil. The use of residues as fuel makes oil extraction plants self-sufficient in energy and produces surplus electricity. A large part of the plant nutrients can be recycled by composting empty fruit bunches, with palm oil mill effluent and ashes added. Smallholders contribute close to 50% of the palm oil traded worldwide. The crop has acquired a bad reputation, however, because of the forests which have been and continue to be cleared for new plantations. The rate of forest clearing has been particularly high in Indonesia and Malaysia, with Thailand and some African countries following at a distance. Forests on peat soils are also increasingly used, releasing even larger amounts of CO2 from peat decomposition. In the ecologies where oil palm thrives, vast expanses of speargrass ( Imperata cylindrica) have resulted from logging and injudicious land use, including poorly managed plantations. Although speargrass is hard to get rid of, a sequence of annual and perennial plant species is capable to suppress the grass, opening a window for planting oil palm in the degraded land. A well-established oil palm crop will then keep the grass out, because of the latter’s intolerance for shade. Thus, the oil palm’s image in the eyes of the public as an ecological disaster can be converted into its opposite: that of a tool to restore the productive capacity of degraded lands. Vast numbers of smallholders can thereby safeguard their livelihood. The oil palm industry has the means and skills to make this happen.


2010 ◽  
Vol 27 (4) ◽  
pp. 289-299 ◽  
Author(s):  
Subhi Siti Masura ◽  
Ghulam Kadir Ahmad Parveez ◽  
Ismanizan Ismail

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