scholarly journals The effect of sulphates on partitioning of pectinases in aqueous two-phase systems

2004 ◽  
pp. 179-186 ◽  
Author(s):  
Mirjana Antov ◽  
Draginja Pericin ◽  
Biljana Trbojevic
Keyword(s):  
Polyethylene Glycol ◽  
Partition Coefficient ◽  
Ammonium Sulphate ◽  
Line Length ◽  
Sodium Sulphate ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Sulphate Salts ◽  
Tie Line

The effect of various sulphate salts on the partitioning of endo-pectinase and exo-pectinase in aqueous two-phase systems, composed of polyethylene glycol and dextran, was studied. Presence of ammonium sulphate and sodium sulphate at concentration 15 mmol/l in the system polyethylene glycol 4000/crude dextran, at tie-line length 7.44%, increased partition coefficient of endo-pectinase 1.25 and 1.2 fold, respectively. Ammonium sulphate at 15 mmol/l and sodium sulphate at 5 mmol/l enhanced partition coefficient for exo-pectinase for about 60% in comparison to the system without salts. Addition of magnesium and sodium sulphate to a final concentration of 0.3 mmol/l in the system containing polyethylene glycol 6000/dextran 500 000, at tie-line length 6.26%, increased the partition coefficient of endo activity for 95% and 32%, respectively. Both salts at the same concentration increased partition coefficient of exo activity 1.5 and 3 times, respectively, in comparison to the system without salts.

scholarly journals Phase Separation Behavior and System Properties of Aqueous Two-Phase Systems with Polyethylene Glycol and Different Salts: Experiment and Correlation

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Haihua Yuan ◽  
Yang Liu ◽  
Wanqian Wei ◽  
Yongjie Zhao
Keyword(s):  
Phase Separation ◽  
Sodium Citrate ◽  
Line Length ◽  
Continuous Phase ◽  
Two Phase ◽  
Separation Rate ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
System Properties ◽  
Tie Line

The phase separation behaviors of PEG1000/sodium citrate, PEG4000/sodium citrate, PEG1000/ammonium sulfate, and PEG4000/ammonium sulfate aqueous two-phase systems were investigated, respectively. There are two distinct situations for the phase separation rate in the investigated aqueous two-phase systems: one state is top-continuous phase with slow phase separation rate and strong bottom-continuous phase with fast phase separation rate and weak volume ratio dependence. The system properties such as density, viscosity, and interfacial tension between top and bottom phases which have effects on the phase separation rate of aqueous two-phase systems were measured. The property parameter differences between the two phases increased with increasing tie line length and then improved the phase separation rate. Moreover, a modified correlation equation including the phase separation rate, tie line length, and physical properties of the four aqueous two-phase systems has been proposed and successfully tested in the bottom-continuous phase, whose coefficients were estimated through regression analysis. The predicted results of PEG1000/sodium citrate aqueous two-phase systems were verified through the stationary phase retention in the cross-axis countercurrent chromatography.

Cell Separation in Aqueous Two-Phase Systems − Influence of Polymer Molecular Weight and Tie-Line Length on the Resolution of Five Model Cell Lines

2017 ◽  
Vol 13 (2) ◽  
pp. 1700250 ◽  
Author(s):  
Sarah Zimmermann ◽  
Sarah Gretzinger ◽  
Philipp K. Zimmermann ◽  
Are Bogsnes ◽  
Mattias Hansson ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Cell Lines ◽  
Cell Separation ◽  
Line Length ◽  
Polymer Molecular Weight ◽  
Two Phase ◽  
Model Cell ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Tie Line

Porcine pancreatic lipase partition in potassium phosphate–polyethylene glycol aqueous two-phase systems

2007 ◽  
Vol 859 (2) ◽  
pp. 222-228 ◽  
Author(s):  
Georgina Bassani ◽  
Beatriz Farruggia ◽  
Bibiana Nerli ◽  
Diana Romanini ◽  
Guillermo Picó
Keyword(s):  
Polyethylene Glycol ◽  
Pancreatic Lipase ◽  
Potassium Phosphate ◽  
Porcine Pancreatic Lipase ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

scholarly journals Purification of microbial b-galactosidase from Kluyveromyces fragilis by bioaffinity partitioning

1999 ◽  
Vol 30 (4) ◽  
pp. 324-331 ◽  
Author(s):  
Maria Estela da Silva ◽  
Telma Teixeira Franco
Keyword(s):  
Partition Coefficient ◽  
Second Step ◽  
Step Method ◽  
Kluyveromyces Fragilis ◽  
Two Phase ◽  
Rich Phase ◽  
Extraction And Purification ◽  
Peg 4000 ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

This work investigated the partitioning of b-galactosidase from Kluyveromyces fragilis in aqueous two-phase systems (ATPS) by bioaffinity. PEG 4000 was chemically activated with thresyl chloride, and the biospecific ligand p-aminophenyl 1-thio-b-D-galactopyranoside (APGP) was attached to the activated PEG 4000. A new two-step method for extraction and purification of the enzyme b-galactosidase from Kluyveromyces fragilis was developed. In the first step, a system composed of 6% PEG 4000-APGP and 8% dextran 505 was used, where b-galactosidase was strongly partitioned to the top phase (K = 2,330). In the second step, a system formed of 13% PEG-APGP and 9% phosphate salt was used to revert the value of the partition coefficient of b-galactosidase (K = 2 x 10-5) in order to provide the purification and recovery of 39% of the enzyme in the bottom salt-rich phase.

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