Genetic structure of black poplar (Populus nigra L.) population in the area of Great War Island

The genetic structure of black poplar (Populus nigra L.) populations in the area of Great War Island (GWI) was studied at the level of 30 genotypes, based on microsatellite molecular markers (SSR). Eleven polymorphic SSR loci were used for analysis of intarpopulation genetic variability. Observed and expected heterozygosity in studied population were high (0.70 and 0.82). The fixation index calculated for single locus ranged from -0.055 (PMGC_14) up to 0.424 (PMGC_2607), while the mean value was 0.143. Deviation from Hardy-Weinberg equilibrium (HWE) differed between single loci. Stabile genetic structure and satisfactory level of genetic variability that have been determined at the population level represent a good starting point for conservation and sustainable use of the available gene pool and further breeding of this species.

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Genetic structure of remnant black poplar (Populus nigra L.) populations along biggest rivers in Serbia assessed by SSR markers

Silvae Genetica ◽

10.1515/sg-2016-0002 ◽

2016 ◽

Vol 65 (1) ◽

pp. 12-19 ◽

Cited By ~ 2

Author(s):

Dijana Čortan ◽

Hilke Schroeder ◽

Mirjana Šijačić-Nikolić ◽

Christian Wehenkel ◽

Matthias Fladung

Keyword(s):

Genetic Variation ◽

Genetic Structure ◽

Natural Habitat ◽

Allelic Diversity ◽

Keystone Species ◽

Significant Loss ◽

Populus Nigra ◽

Fixation Index ◽

Black Poplar ◽

Western Asia

AbstractBlack poplar (Populus nigra L.) is a keystone species of riparian softwood forests along riversides in vast areas of Europe, Western Asia and Northern Africa. Since the end of the 20thcentury, black poplar has been recognized as an endangered species throughout Europe due to the loss of its natural habitat and possible crossbreeding with hybrid poplars. Using twelve nuclear SSR loci, we analysed the genetic structure of four native populations from three river valleys in the northern part of Serbia. All tested loci were highly polymorphic, displaying 8 to 25 alleles per locus, overall 179 detected alleles and an average effective number of alleles 5.87. Observed heterozygosity (overall Ho = 0.703) has been lower than the expected (overallHe= 0.808) in each population, which indicates positive mean of fixation index values (overallFis> 0 (0.132)). An AMOVA analysis revealed that the highest degree of genetic variation occurred within populations (95.33 %) while the genetic variation between populations was really low (4.67 %). High gene flow and no significant loss of allelic diversity have been recorded in the studied populations in Serbia.

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Morphometric characteristics of black poplar (Populus nigra L.) leaves in the area of Great War Island

Glasnik Sumarskog fakulteta ◽

10.2298/gsf1308093m ◽

2013 ◽

pp. 93-108 ◽

Cited By ~ 1

Author(s):

Zoran Maksimovic ◽

Mirjana Sijacic-Nikolic

Keyword(s):

Natural Populations ◽

Populus Nigra ◽

Ex Situ ◽

Great War ◽

Morphometric Characteristics ◽

Evolutionary Potential ◽

Ecological Adaptability ◽

Black Poplar ◽

Starting Point ◽

Conservation Of Genetic Resources

This paper presents the results of an analysis of the morphometric characteristics of the leaves of 14 test black poplar (Populus nigra L.) trees, from the region of Great War Island, indicating a satisfactory degree of intrapopulation genetic variability. The knowledge of the extent and nature of variability in natural populations is a starting point for the breeding and conservation of genetic resources of forest trees. To this end, it is necessary to begin with the implementation of appropriate measures of in situ and ex situ conservation that will enable long-term preservation and enhancement of the ecological adaptability and the evolutionary potential of the populations of black poplar on Great War Island.

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Analysis of Genetic Diversity in three Kazakh Sheep using 12 Microsatellites

International Journal of Engineering & Technology ◽

10.14419/ijet.v7i4.38.24336 ◽

2018 ◽

Vol 7 (4.38) ◽

pp. 122

Author(s):

Kairat Dossybayev ◽

Aizhan Mussayeva ◽

Bakytzhan Bekmanov ◽

Beibit Kulataev

Keyword(s):

Genetic Diversity ◽

Genetic Variability ◽

Mean Value ◽

Fixation Index ◽

Merino Sheep ◽

Str Markers ◽

Sheep Breeds ◽

Str Loci ◽

High Level ◽

Two Populations

The genetic structure of three Kazakh sheep breeds was examined by using 12 microsatellite loci. A total of 144 alleles were detected from the 12 STR loci, with a mean value of 12.0. The highest allele diversity was found at the locus CSRD247 (16 alleles). PIC value showed that all studied STR markers are more informative and appropriate for genetic analysis of three Kazakh sheep populations. Beside of INRA006, all markers had high level of genetic variability. As Fixation index shows, the excess of the heterozygosity was observed only in loci MAF065. Obtained number of private alleles in Edilbai, Kazakh Arkhar Merino and Kazakh Fine-wool sheep were 25, 17 and 15 respectively. Genetic diversity was higher in Edilbai population than in other two populations. The genetic variability was lower in Kazakh Arkhar Merino sheep than in the Edilbai and Kazakh Fine-wool sheep breeds. The genetic distance was the largest between Edilbai and Kazakh Arkhar Merinos. Also, the moderate differentiation was observed between Edilbai and Kazakh Arkhar Merinos.

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Conservation status of white poplar (Populus alba L.) and black poplar (Populus nigra L.) in the territory of Great War Island

Glasnik Sumarskog fakulteta ◽

10.2298/gsf1409169s ◽

2014 ◽

pp. 169-180

Author(s):

Mirjana Sijacic-Nikolic ◽

Jelena Milovanovic ◽

Marina Nonic ◽

Zoran Maksimovic ◽

Dijana Cortan

Keyword(s):

Gene Pool ◽

Forest Ecosystems ◽

Conservation Status ◽

Populus Nigra ◽

Ex Situ ◽

Great War ◽

Populus Alba ◽

White Poplar ◽

Black Poplar

T he paper describes t he conservation status of white poplar (Populus alba L.) and black poplar (Populus nigra L.) in the territory of Great War Island. The activities of in situ and ex situ gene pool conservation have been defined in order to preserve and expand the populations of the above species, as carriers of complex wetland forest ecosystems.

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Genetic Structure in Populations of Euterpe precatoria Mart. in the Brazilian Amazon

Frontiers in Ecology and Evolution ◽

10.3389/fevo.2020.603448 ◽

2021 ◽

Vol 8 ◽

Author(s):

Santiago Linorio Ferreyra Ramos ◽

Gabriel Dequigiovanni ◽

Maria Teresa Gomes Lopes ◽

Ananda Virginia de Aguiar ◽

Ricardo Lopes ◽

...

Keyword(s):

Genetic Structure ◽

Bayesian Analysis ◽

Management Strategies ◽

Geographic Distance ◽

Natural Populations ◽

Population Level ◽

Brazilian Amazon ◽

The State ◽

Fixation Index ◽

Palm Tree

Euterpe precatoria is a palm tree belonging to the Arecaceae family, occurring in Western and Central Brazilian Amazonia. Its fruit, which is very appreciated in the Amazon region, produces pulp that is consumed in fresh form. Its production is carried out almost exclusively by extractive farmers. In order to establish adequate strategies to sustain this genetic resource, we need knowledge about the diversity and genetic structure in natural populations. This study aimed to evaluate the influence of geographic distance on genetic structure in the main extractive populations of E. precatoria in the Brazilian Amazon. Leaves from 377 plants were collected in 19 populations located in 16 municipalities in the State of Amazonas and three in the State of Rondônia. Twelve microsatellite loci were used to genotype the plants. The diversity and genetic structure among populations were estimated. The average number of alleles per locus was 5.97. The observed heterozygosity means (HO) were higher than expected (HE) at the population level (HO = 0.72, HE = 0.66) and fixation index (f = -0.100) was negative. The FST value (0.1820) and the AMOVA results (Φ = 0.1796) showed population structure. The populations were clustered into three groups (K = 3) in the Bayesian analysis. The Discriminant Analysis of Principal Components (DAPC) confirmed eight clusters, with the populations close to those identified by the Bayesian analysis. The geographic differentiation was confirmed by the groupings obtained in the Structure analysis and the DACP function. Information related to phenotypic, genetic and environmental characterization of populations is important to guide conservation and management strategies and the formulation of public species management policies in Amazonia.

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Diversity and Genetic Structure of Dioon holmgrenii (Cycadales: Zamiaceae) in the Mexican Pacific Coast Biogeographic Province: Implications for Conservation

Plants ◽

10.3390/plants10112250 ◽

2021 ◽

Vol 10 (11) ◽

pp. 2250

Author(s):

Mario Valerio Velasco-García ◽

Carlos Ramírez-Herrera ◽

Javier López-Upton ◽

Juan Ignacio Valdez-Hernández ◽

Higinio López-Sánchez ◽

...

Keyword(s):

Genetic Diversity ◽

Genetic Structure ◽

Pacific Coast ◽

Population Level ◽

Fixation Index ◽

High Genetic Diversity ◽

Mexican Pacific ◽

Biogeographic Province ◽

Multiple Factors ◽

F Statistics

Dioon holmgrenii De Luca, Sabato et Vázq.Torres is an endangered species; it is endemic and its distribution is restricted to the biogeographic province of the Mexican Pacific Coast. The aim of this work was to determine the diversity and genetic structure of nine populations. The genetic diversity parameters and Wright’s F statistics were determined with six microsatellite loci. The genetic structure was determined by using the Structure software and by a discriminant analysis. The genetic diversity of the populations was high. The proportion of polymorphic loci was 0.89, the observed heterogeneity was higher (Ho = 0.62 to 0.98) than expected (He = 0.48 to 0.78), and the fixation index was negative (IF = −0.091 to −0.601). Heterozygous deficiency (FIT = 0.071) was found at the species level and heterozygotes excess (FIS = −0.287) at the population level. The genetic differentiation between populations was high (FST = 0.287), with the number of migrants less than one. Three groups of populations were differentiated, and the variation within populations, between populations, and between groups was: 65.5, 26.3, and 8.2%, respectively. Multiple factors explain the high genetic diversity, while the genetic structure is due to geographic barriers. Community reserves are urgent in at least one most diverse population of each group.

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In situ conservation of black poplar (Populus nigra L.) gene pool in the protected area “Great War Island”

REFORESTA ◽

10.21750/refor.2.05.20 ◽

2016 ◽

pp. 39-49 ◽

Cited By ~ 1

Author(s):

Zoran Maksimović ◽

◽

Mirjana Šijačić-Nikolić ◽

Keyword(s):

Protected Area ◽

Gene Pool ◽

In Situ Conservation ◽

Populus Nigra ◽

Great War ◽

Black Poplar

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CONNECTION g.307 G > A SNP POLYMORPHISM OF THE ALPHA-FUKOZYLTRASFERAZA 1 GENE WITH ECONOMIC-USEFUL TRAITS IN THE LARGE WHITE PIG BREED

Animal Breeding and Genetics ◽

10.31073/abg.54.17 ◽

2017 ◽

Vol 54 ◽

pp. 134-140

Author(s):

H. S. Rudoman ◽

V. M. Balatsky ◽

V. Y. Nor ◽

V. O. Vovk

Keyword(s):

Genetic Structure ◽

Information Content ◽

Analysis Of Variance ◽

Farm Animals ◽

Daily Weight Gain ◽

Fixation Index ◽

Its Sequencing ◽

Large White ◽

Stage Of Development ◽

High Level

One of the top priorities at the present stage of development of pig breeding remains the development of a set of measures aimed at increasing the resistance animals to various diseases, especially – to colibacteriosis .One of the recent and effective approaches to prevent colibacillosis is using markers of selection; it involves pig genotyping by genome locuses. Chosen locuses are associated with animal sensitivity to the disease and selection of the results of genotyping of animals with increased resistance. Due to researches, one of such locuses is alpha-fukozyltrasferаza 1 gene (FUT1). Gene FUT1 is located in chromosome 6. As a result of its sequencing in the swine breeds of Large White and Swedish Landrace, single-nucleotide polymorphism (g.307 G > A SNP) has been detected. AA genotype determines the resistance of animals to colibacteriosis, while AG and GG genotypes are susceptible to this disease. According to the results of previous studies, the positive effect of allele A was determined not only on the resistance of pigs to colibacteriosis, but also on indicators of fattening and meat productivity and on reproductive performance. In Ukraine, the studies of polymorphism FUT1 g.307 G > A SNP were held fragmentedly and only on certain populations of Ukrainian Meat and Large White breed but without establishing its association with the indicators of productivity of pigs. The aim of our work was to study the genetic structure of Ukrainian Large White breeds, type 1 and the establishment of association. g. 307 G > A SNP FUT1 gene with pigs indicators of productivity. For research the 96 samples of hair were used. DNA isolation from samples with biomaterial were carried out using ion exchange resin Chelex-100. Genotyping was performed by PCR-RFLP by method of Jorgensen et al. (2006). Using DNA analysis of this breeds locus FUT1 was determined by genetic structure. Allele frequency of allele G (0,573) and allele A (0,427) was established The distribution of genotype frequencies was not statistically significantly different from the theoretically expected, calculated by the Hardy-Weinberg criterion. Thus, according to the locus of FUT1 g.307 G > A, the breeds investigated are in a state close to the genetic equilibrium. The negative value of the fixation index by the locus FUT1 g.307 G > A indicates an excess of heterozygotes in the population, and hence the lack of targeted selection for this marker. The valuation was performed by calculating the PIC (polymorphic information content) – information content of polymorphism marker. In the analyzed pig herd for this marker, the PIC has an average value (0,367), which indicates the high level of polymorphism of the given locus and is favorable for the possibility of conducting a search for links between individual genotypes and indicators of productivity. To establish the association g.307 G > A SNP FUT1 gene with indicators of animal productivity, a one-way analysis of variance of the experimental data was used. Based on the results of a one-way analysis of variance, a significant effect of the genotypes of the FUT1gene (g.1849 G > C) on the indicator of the average daily weight gain (р ≤ 0,001), the thickness of the bacon at the level of the VI-VII vertebrae (p ≤ 0.01), reaching live weight of 100 kg (р ≤ 0,001) and breeding index of fattening qualities (р ≤ 0,01) was found. The parameter of the influence of the genetic factor on the test feature was 28,54%, 10,4%, 33,4% and 8,8% respectively. According to the investigated indicators of productivity, animals with genotype AA, which determines resistance to colibacteriosis, dominated the animals with genotypes GG and AG. Based on the results of our research and pre-published data, the multiple effect of the g.307 G > A SNP FUT1 gene is observed, which is associated with the indicators of productivity, which again confirms the polygenicity of the quantitative trait loci of farm animals. Taking into account a high level of polymorphism of the investigated gene and reliable associations of genotypes with indicators of productivity found, it can be recommended to carry out breeding of pigs using genetic information of the g.307 G > A SNP gene FUT1.

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Low effective population size and high spatial genetic structure of black poplar populations from the Oder valley in Poland

Annals of Forest Science ◽

10.1007/s13595-021-01055-2 ◽

2021 ◽

Vol 78 (2) ◽

Author(s):

Błażej Wójkiewicz ◽

Andrzewj Lewandowski ◽

Weronika B. Żukowska ◽

Monika Litkowiec ◽

Witold Wachowiak

Keyword(s):

Genetic Structure ◽

Genetic Resources ◽

Population Size ◽

Effective Population Size ◽

Spatial Genetic Structure ◽

Demographic History ◽

River Valley ◽

Ex Situ ◽

Effective Population ◽

Black Poplar

Abstract Context Black poplar (Populus nigra L.) is a keystone species of European riparian ecosystems that has been negatively impacted by riverside urbanization for centuries. Consequently, it has become an endangered tree species in many European countries. The establishment of a suitable rescue plan of the remaining black poplar forest stands requires a preliminary knowledge about the distribution of genetic variation among species populations. However, for some parts of the P. nigra distribution in Europe, the genetic resources and demographic history remain poorly recognized. Aims Here, we present the first study on identifying and characterizing the genetic resources of black poplar from the Oder valley in Poland. This study (1) assessed the genetic variability and effective population size of populations and (2) examined whether gene flow is limited by distance or there is a single migrant pool along the studied river system. Methods A total of 582 poplar trees derived from nine black poplar populations were investigated with nuclear microsatellite markers. Results (1) The allelic richness and heterozygosity level were high and comparable between populations. (2) The genetic structure of the studied poplar stands was not homogenous. (3) The signatures of past bottlenecks were detected. Conclusion Our study (1) provides evidence for genetic substructuring of natural black poplar populations from the studied river catchment, which is not a frequent phenomenon reported for this species in Europe, and (2) indicates which poplar stands may serve as new genetic conservation units (GCUs) of this species in Europe. Key message The genetic resources of black poplar in the Oder River valley are still substantial compared to those reported for rivers in Western Europe. On the other hand, clear signals of isolation by distance and genetic erosion reflected in small effective population sizes and high spatial genetic structure of the analyzed populations were detected. Based on these findings, we recommend the in situ and ex situ conservation strategies for conserving and restoring the genetic resources of black poplar populations in this strongly transformed by human river valley ecosystem.

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