scholarly journals Cytogenetic analysis of bone marrow cells of rats treated with toluene

2004 ◽  
Vol 58 (3-4) ◽  
pp. 311-318
Author(s):  
Svetlana Fister ◽  
Slavoljub Jovic ◽  
Jelka Stevanovic ◽  
Milica Kovacevic-Filipovic
Keyword(s):  
Bone Marrow ◽  
Mitotic Index ◽  
Cytogenetic Analysis ◽  
Wistar Rats ◽  
Bone Marrow Cells ◽  
Experimental Conditions ◽  
Structural Aberrations ◽  
Female Wistar Rats ◽  
Cytogenetic Analyses ◽  
Marrow Cells

The paper presents the results of investigations of the effect of toluene on bone marrow cells of female Wistar rats treated intraperitoneally with toluene for 8 or 11 days, in doses of 0.602 ?g/200 g body mass. Cytogenetic analyses were performed on the metaphase figure of chromosomes in order to determine the frequency of structural aberrations ? breaks and gaps. The values of the mitotic index and number of poliploid cells were determined. No significant increase was determined in the frequency of breaks and gaps in chromosomes of treated animals in comparison with the controls, which means that, under the experimental conditions, toluene did not exhibit a definite genotoxic effect. However, it has been determined that there was a significant increase in the value of the mitotic index, as well as a significant increase in the number of poliploid cells in both groups of treated rats in comparison with controls.

Antigenotoxic effect of genistein against 7,12-dimethylbenz[a]anthracene induced genotoxicity in bone marrow cells of female wistar rats

2009 ◽  
Vol 61 (2) ◽  
pp. 296-303 ◽  
Author(s):  
Pachaiappan Pugalendhi ◽  
Shanmugam Manoharan ◽  
Kuppusamy Panjamurthy ◽  
Subramanian Balakrishnan ◽  
Madhavan R. Nirmal
Keyword(s):  
Bone Marrow ◽  
Wistar Rats ◽  
Bone Marrow Cells ◽  
Female Wistar Rats ◽  
Antigenotoxic Effect ◽  
Marrow Cells

scholarly journals Effect of aqueous extract of Rosemary officinalis on cytotoxicity of CCL4 induced albino male mice

2018 ◽  
Vol 12 (1) ◽  
pp. 124-131
Author(s):  
Ruqaya M. Ibrahim
Keyword(s):  
Bone Marrow ◽  
Mitotic Index ◽  
Aqueous Extract ◽  
Bone Marrow Cells ◽  
Post Treatment ◽  
Rosemary Extract ◽  
Mutagenic Action ◽  
Male Mice ◽  
Micronucleus Formation ◽  
Marrow Cells

This study focused the line on the effect of aqueous extract of Rosemary officinalis, as well as, effect of toxic compound CCL4, on micronucleus formation and mitotic index assay in albino male mice. This work started at September 2017 at Biotechnology Research center \Al-Nahrain University, by using 20 albino male mice. The result indicated that aqueous extract of rosemary caused significant increased in mitotic index and decrease micronucleus formation for two doses tested 50,100 mg/kg in comparison with negative and positive controls, also the results revealed that CCL4 showed significant mutagenic action on biological system of treated mice by increased frequency of micronucleus formation and decreased the percentage of mitotic index in bone marrow cells. Pre-and post –treatment between aqueous extract and CCL4 were also made. The results of pre and post treatment with rosemary extract were also caused a significant decreased in micronucleus formation and increase the percentage of mitotic index for two doses 50,100 mg/kg  in comparison with its corresponding controls which caused increased in the frequencies of micronucleus formation and decrease the percentage of mitotic index in bone marrow cells. Conclusions: Rosemary officinalis enhanced immunity, reduced mutagenic effects against cytotoxicity of CCL4.

The Effects of Yerba Maté (Ilex Paraguariensis) Consumption on IL-1, IL-6, TNF-α and IL-10 Production by Bone Marrow Cells in Wistar Rats fed a High-Fat Diet

2013 ◽  
Vol 83 (1) ◽  
pp. 26-35 ◽  
Author(s):  
Luciana Simão Carmo ◽  
Marcelo Macedo Rogero ◽  
Mayara Cortez ◽  
Monica Yamada ◽  
Patrícia Silva Jacob ◽  
...  
Keyword(s):  
Bone Marrow ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Bone Marrow Cells ◽  
The Body ◽  
Ilex Paraguariensis ◽  
Yerba Mate ◽  
High Fat ◽  
Tnf Α ◽  
Marrow Cells

An excessive consumption of a high-fat diet (HFD) results in becoming overweight or obese, which triggers a chronic inflammatory condition that is associated with a high white blood cell count. Because of the potential for yerba maté (Ilex paraguariensis) (YM) to impact obesity, this study aimed to investigate the effects of YM consumption on the hematological response and on the production of interleukin (IL)-1α, IL-6, tumor necrosis factor (TNF)-α, and IL-10 by bone marrow cells from Wistar rats fed a HFD. Male Wistar rats were fed a control (CON) or HFD diet for twelve weeks. At the end of this period, the rats received YM (1 g/kg/day body weight) for 4 weeks. After euthanasia, hemograms and myelograms were evaluated, while the bone marrow cells were cultured in the presence or absence of lipopolysaccharide (LPS) to evaluate the production of IL-1α, IL-6, TNF-α, and IL-10. The consumption of YM reduced the body weight, the body adiposity, and the cholesterol levels in HFD-fed rats. Bone marrow cells from the HFD group produced more IL-1α, IL-6, and TNF-α, and less IL-10, when compared to cells from the control group, and YM consumption reduced the IL-1α, IL-6, and TNF-α production by the cells. However, cells from the HFD rats that were stimulated with LPS increased their IL-1α, IL-6, and TNF-α production, but YM consumption did not change this result. In summary, the consumption of YM affects the production of IL-1α, IL-6, and TNF-α by bone marrow cells, promotes weight loss, decreases the number of white blood cells, and significantly improves serum cholesterol level in HFD-fed rats. However, the bone marrow cells from the HFD+YM-fed rats challenged with LPS did not show improvement in the inflammatory response compared to the cells from animals fed only a HFD that were also challenged with LPS.

scholarly journals Absence of Genotoxic and Antigenotoxic Effects of a Standardized Extract of the Medicinal Plant Solanum melongena on Peripheral Blood and Bone Marrow Cells of Wistar Rats.

CYTOLOGIA ◽  
2002 ◽  
Vol 67 (4) ◽  
pp. 417-422 ◽  
Author(s):  
Daniele Roswell Chacon ◽  
Andreia Nappo Dalla Libera ◽  
Dennys Esper Correia Cintra ◽  
José Carlos Tavares Carvalho ◽  
Gilberto Antonio de Oliveira ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Medicinal Plant ◽  
Peripheral Blood ◽  
Wistar Rats ◽  
Bone Marrow Cells ◽  
Solanum Melongena ◽  
Antigenotoxic Effects ◽  
Marrow Cells

Could Cobalamin Deficiency Mimic a Clonal Aberration Cytogenetic? a Case Report

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 4882-4882
Author(s):  
Marcelo Bellesso ◽  
Daniela Ferreira Dias ◽  
Renato Torrescasana Centrone ◽  
Laura Yolanda Chialanza Garcia ◽  
Annelise Correa Wengerkievicz Lopes ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Vitamin B12 ◽  
Cytogenetic Analysis ◽  
Bone Marrow Aspirate ◽  
Bone Marrow Cells ◽  
Megaloblastic Anemia ◽  
Cobalamin Deficiency ◽  
Normal Range ◽  
Clonal Deletion ◽  
Marrow Cells

Abstract A 32 years old female patient presented to our service complaining asthenia, apathy and loss of her power to work. It was observed, by laboratory test, pancytopenia (Hb: 6.0 g/dL; WBC: 2,100/mm³, platelets 95,000/mm³, Reticulocytes 0.8%) associated with marked elevation of LDH 1,800 U/L (normal range: 240 – 480U/L). Bone marrow aspirate demonstrated morphologic features of megaloblastic anemia. Moreover, low serum concentration of vitamin B12 < 150pg/mL (normal range: 200 – 95-pg/mL), Folic acid 15,9ng/mL (normal range: 3 – 17 ng/mL) confirmed Megaloblastic anemia by cobalamin deficiency. It was not evidenced gastritis. It was initiated the treatment with vitamin B12. However, in the next clinical attendance it was observed an unexpected cytogenetic result: 46,XX,del(9)(q13q22)[3]/76~78,XXX,+1,+3,+4,+9,+11,+12,+17,+20,+21[cp3]/46,XX[34]. It was interpreted that del(9)(q13q22)[3] could be a clonal cytogenetics aberration and the others data due to defects in synthesis of DNA by cobalamin deficiency. It was important, because in diagnosis of Megaloblastic anemia frequently it is not included cytogenetic analysis of bone marrow cells and a new cytogenetic analysis has become necessary due to this data. After two months, a complete hematological recovery was achieved and six months later, bone marrow aspirate and cytogenetic analisys were repeated. Normal morphologic bone marrow cells and normal cytogenetic: 46,XX[20] were evidenced. Therefore, it was really hard to conclude this case. First, cobalamin deficiency could promote this clonal deletion or, as a second hypothesis, a clonal cytogenetic with low proliferative rate was selected due to inefficient hematopoiesis, by vitamin B12 deficiency, and after the recovery of the hematopoiesis, this clonal was suppressed. Disclosures No relevant conflicts of interest to declare.

scholarly journals Potential chemoprotective effects of green propolis, L-lysine and celecoxib on bone marrow cells and peripheral blood lymphocytes of Wistar rats subjected to bladder chemical carcinogenesis

2014 ◽  
Vol 29 (7) ◽  
pp. 423-428 ◽  
Author(s):  
Conceição Aparecida Dornelas ◽  
Bruno Coelho Cavalcanti ◽  
Hemerson Iury Ferreira Magalhães ◽  
Francisco Vagnaldo Fechine Jamacaru ◽  
Francisco Nelson Nóbrega Furtado ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Wistar Rats ◽  
Bone Marrow Cells ◽  
Chemical Carcinogenesis ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Marrow Cells

scholarly journals Anticlastogenic effect of vitamin C on cisplatin in vivo

1999 ◽  
Vol 22 (3) ◽  
pp. 415-417 ◽  
Author(s):  
Lusânia M. Greggi Antunes ◽  
Joana D.C. Darin ◽  
Maria de Lourdes P. Bianchi
Keyword(s):  
Bone Marrow ◽  
Body Weight ◽  
Vitamin C ◽  
Wistar Rats ◽  
Bone Marrow Cells ◽  
Chemotherapeutic Agent ◽  
Anticlastogenic Effect ◽  
Rat Bone ◽  
Marrow Cells

The ability of vitamin C (VC) to protect against the clastogenic action of the chemotherapeutic agent cisplatin (DDP, cis-diamminedichloroplatinun II) in rat bone marrow cells was evaluated. DDP was administered to Wistar rats either alone or after treatment with VC. The rats were treated with VC (50, 100 or 200 mg/kg body weight) by gavage 10 min before the administration of DDP (5 mg/kg body weight, ip) and then sacrificed 24 h after treatment. VC significantly reduced (by about 70%) the clastogenicity of DDP in rat bone marrow cells. The antioxidant action of VC presumably modulates the clastogenic action of DDP.

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