scholarly journals EFFECT OF CARROT JUICE (DAUCUS CAROTA ) ON MITOTIC INDEX AND FORMATION OF MICRONUCLEI IN FEMUR BONE MARROW CELLS OF WHITE MICE

2008 ◽  
Vol 36 (2) ◽  
pp. 108-116
Author(s):  
Ilham A. Khalaf
Keyword(s):  
Bone Marrow ◽  
Mitotic Index ◽  
Daucus Carota ◽  
Bone Marrow Cells ◽  
Carrot Juice ◽  
Femur Bone ◽  
Marrow Cells

scholarly journals Effect of aqueous extract of Rosemary officinalis on cytotoxicity of CCL4 induced albino male mice

2018 ◽  
Vol 12 (1) ◽  
pp. 124-131
Author(s):  
Ruqaya M. Ibrahim
Keyword(s):  
Bone Marrow ◽  
Mitotic Index ◽  
Aqueous Extract ◽  
Bone Marrow Cells ◽  
Post Treatment ◽  
Rosemary Extract ◽  
Mutagenic Action ◽  
Male Mice ◽  
Micronucleus Formation ◽  
Marrow Cells

This study focused the line on the effect of aqueous extract of Rosemary officinalis, as well as, effect of toxic compound CCL4, on micronucleus formation and mitotic index assay in albino male mice. This work started at September 2017 at Biotechnology Research center \Al-Nahrain University, by using 20 albino male mice. The result indicated that aqueous extract of rosemary caused significant increased in mitotic index and decrease micronucleus formation for two doses tested 50,100 mg/kg in comparison with negative and positive controls, also the results revealed that CCL4 showed significant mutagenic action on biological system of treated mice by increased frequency of micronucleus formation and decreased the percentage of mitotic index in bone marrow cells. Pre-and post –treatment between aqueous extract and CCL4 were also made. The results of pre and post treatment with rosemary extract were also caused a significant decreased in micronucleus formation and increase the percentage of mitotic index for two doses 50,100 mg/kg  in comparison with its corresponding controls which caused increased in the frequencies of micronucleus formation and decrease the percentage of mitotic index in bone marrow cells. Conclusions: Rosemary officinalis enhanced immunity, reduced mutagenic effects against cytotoxicity of CCL4.

scholarly journals Cytogenetic analysis of bone marrow cells of rats treated with toluene

2004 ◽  
Vol 58 (3-4) ◽  
pp. 311-318
Author(s):  
Svetlana Fister ◽  
Slavoljub Jovic ◽  
Jelka Stevanovic ◽  
Milica Kovacevic-Filipovic
Keyword(s):  
Bone Marrow ◽  
Mitotic Index ◽  
Cytogenetic Analysis ◽  
Wistar Rats ◽  
Bone Marrow Cells ◽  
Experimental Conditions ◽  
Structural Aberrations ◽  
Female Wistar Rats ◽  
Cytogenetic Analyses ◽  
Marrow Cells

The paper presents the results of investigations of the effect of toluene on bone marrow cells of female Wistar rats treated intraperitoneally with toluene for 8 or 11 days, in doses of 0.602 ?g/200 g body mass. Cytogenetic analyses were performed on the metaphase figure of chromosomes in order to determine the frequency of structural aberrations ? breaks and gaps. The values of the mitotic index and number of poliploid cells were determined. No significant increase was determined in the frequency of breaks and gaps in chromosomes of treated animals in comparison with the controls, which means that, under the experimental conditions, toluene did not exhibit a definite genotoxic effect. However, it has been determined that there was a significant increase in the value of the mitotic index, as well as a significant increase in the number of poliploid cells in both groups of treated rats in comparison with controls.

scholarly journals Assessment of Genotoxic and Cytotoxic Hazards in Brain and Bone Marrow Cells of Newborn Rats Exposed to Extremely Low-Frequency Magnetic Field

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Monira M. Rageh ◽  
Reem H. EL-Gebaly ◽  
Nihal S. El-Bialy
Keyword(s):  
Magnetic Field ◽  
Bone Marrow ◽  
Dna Damage ◽  
Mitotic Index ◽  
Bone Marrow Cells ◽  
Low Frequency ◽  
Newborn Rats ◽  
Extremely Low Frequency ◽  
Frequency Magnetic Field ◽  
Marrow Cells

The present study aimed to evaluate the association between whole body exposure to extremely low frequency magnetic field (ELF-MF) and genotoxic , cytotoxic hazards in brain and bone marrow cells of newborn rats. Newborn rats (10 days after delivery) were exposed continuously to 50 Hz, 0.5 mT for 30 days. The control group was treated as the exposed one with the sole difference that the rats were not exposed to magnetic field. Comet assay was used to quantify the level of DNA damage in isolated brain cells. Also bone marrow cells were flushed out to assess micronucleus induction and mitotic index. Spectrophotometric methods were used to measure the level of malondialdehyde (MDA) and the activity of glutathione (GSH) and superoxide dismutase (SOD). The results showed a significant increase in the mean tail moment indicating DNA damage in exposed group (P<0.01,0.001,0.0001). Moreover ELF-MF exposure induced a significant (P<0.01,0.001) four folds increase in the induction of micronucleus and about three folds increase in mitotic index (P<0.0001). Additionally newborn rats exposed to ELF-MF showed significant higher levels of MDA and SOD (P<0.05). Meanwhile ELF-MF failed to alter the activity of GSH. In conclusion, the present study suggests an association between DNA damage and ELF-MF exposure in newborn rats.

scholarly journals The radioprotective efficacy of the rat acute-phase protein alpha2-macroglobulin on bone marrow cells

Genetika ◽  
2009 ◽  
Vol 41 (1) ◽  
pp. 29-39
Author(s):  
Mirjana Mihailovic ◽  
Melita Vidakovic ◽  
Nevena Grdovic ◽  
Svetlana Dinic ◽  
Aleksandra Uskokovic ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mitotic Index ◽  
Acute Phase ◽  
Blood Cells ◽  
Bone Marrow Cells ◽  
Acute Phase Protein ◽  
Leukocyte Count ◽  
Experimental Animals ◽  
Phase Protein ◽  
Marrow Cells

The rat acute phase protein ?2-macroglobulin (?2M) plays an important role in the restoration of disrupted homeostasis by inhibiting different types of non-specific proteases and facilitating the transport of cytokines, growth factors and hormones. Previously, we observed that administration of ?2M to experimental animals prior to the infliction of life- threatening trauma in the form of scalding or total-body irradiation, significantly improved their survival rates. The aim of the present work was to evaluate the radioprotective effect on blood cells of ?2M that, when administered 30 min before irradiation with 6.7 Gy (LD50/30), provides 100% survival of experimental animals where in unprotected irradiated rats the said dose results in 50% lethality. We observed that rats pretreated with ?2M, after an initial decline, exhibited complete recovery of the leukocyte count due to the preservation of bone marrow cells, observed as a stable mitotic index. In untreated irradiated rats the decrease of the mitotic index reflected the significant destruction of bone marrow cells that resulted in a protracted decline in the leukocyte count. We conclude that the radioprotection provided by ?2M was in part mediated through cytoprotection of new blood cells produced in the bone marrow.

Fluoride and endosulfan together potentiate cytogenetic effects in Swiss albino mice bone marrow cells

2020 ◽  
pp. 074823372097942
Author(s):  
Anju Sharma ◽  
Placheril John ◽  
Pradeep Bhatnagar
Keyword(s):  
Bone Marrow ◽  
Mitotic Index ◽  
Bone Marrow Cells ◽  
Combination Group ◽  
Saline Control ◽  
Individual Treatment ◽  
Swiss Albino Mice ◽  
Polychromatic Erythrocytes ◽  
Albino Mice ◽  
Marrow Cells

In this study, the cytotoxic potential of fluoride and endosulfan in combination was investigated in Swiss albino mice bone marrow cells using the chromosomal aberration (CA) and micronucleus (MN) test systems. Fluoride (25.1 mg kg−1 body weight [bw] in water) and endosulfan (1.8 mg kg−1 bw by oral intubation) were administered orally alone and in combination (fluoride 25.1 mg kg−1 bw + endosulfan 1.8 mg kg−1 bw) to male Swiss albino mice daily for 30 days. A significant ( p < 0.01) increase in micronuclei (MNs) induction and decreased ratio ( p < 0.01) of polychromatic to normonochromatic erythrocytes (indicators of cytotoxicity) were observed compared with saline controls when animals were given the combination of fluoride and endosulfan. A significant ( p < 0.01) increase in MNs induction and no change in the polychromatic erythrocytes to erythrocyte ratio were also observed when endosulfan was given alone. CAs such as gaps, breaks, fragments, rings, exchanges, and polyploidy were recorded in the bone marrow cells. The mean percent frequency of CAs was increased ( p < 0.01) in all the treated groups compared with the control saline group. In the combination group (F + E), the percent frequencies of CAs were significantly higher (13.875%) compared with those in the individual treatment groups of fluoride (4.375%) and endosulfan (6.25%). The mitotic index was calculated as percentage of dividing cells. A significant ( p < 0.01) decrease in mitotic index was observed in all treated groups compared with controls. In the combination group (F + E), mitotic index was significantly less than ( p < 0.01; 4.1 ± 0.49) the saline control (10.8 ± 0.98). These results indicated that repeated intake of endosulfan through various sources in fluoride affected areas resulted in increased cytotoxic effects. The greater effect in the combination group indicated additive interaction of fluoride and endosulfan in inducing cytotoxicity in Swiss albino mice.

scholarly journals Mitotic Indices of Human Bone Marrow Cells. I. Number and Cytologic Distribution of Mitoses

Blood ◽  
1962 ◽  
Vol 19 (6) ◽  
pp. 743-750 ◽  
Author(s):  
SVEN-ÅGE KILLMANN ◽  
EUGENE P. CRONKITE ◽  
T. M. FLIEDNER ◽  
VICTOR P. BOND
Keyword(s):  
Bone Marrow ◽  
Diurnal Variation ◽  
Mitotic Index ◽  
Cell Lines ◽  
Bone Marrow Cells ◽  
Cell Types ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Maturation Stages ◽  
Marrow Cells

Abstract 1. The diurnal variation of mitotic index of human bone marrow has been studied. A diurnal variation has not been demonstrated but remains subject for further study. 2. Data on the distribution of hone marrow mitoses on cell lines and maturation stages are presented. From this and the gross mitotic index of the bone marrow, the mitotic indices of various cell types are computed.

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