scholarly journals Seroprevalence of leptospirosis in horses in the territory of Belgrade during the period from 1998 to 2008

2009 ◽  
Vol 63 (3-4) ◽  
pp. 163-169 ◽  
Author(s):  
Dragica Vojinovic ◽  
Jadranka Zutic ◽  
Slobodan Stanojevic
Keyword(s):  
Blood Serum ◽  
Antibody Titer ◽  
Single Sample ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Sporadic Cases ◽  
Horse Blood ◽  
The Individual ◽  
Leptospira Species

Serological investigations of leptospirosis in horses were carried out in the epizootic territory of Belgrade during the period from 1998 to 2008. Serological examinations were performed on 1701 blood serum samples of horses from private and sociallyowned stables, riding clubs, hippodromes, and from the individual sector. The number of seropositive horses was 107 (6.29%). The most represented serovarieties of the Leptospira species were: Leptospira grippotyphosa 42 (2.47%), Leptospira icterohaemorrhagiae 19 (1.12%), Leptospira pomona 16 (0.99%), Leptospira canicola 16 (0.94%), Leptospira bataviae 11 (0.65%), and Leptospira australis 2 (0.1%). Antibodies against Leptospira sejroe were not established in a single sample of the examined horse blood serums. The titer of specific antibodies ranged from 1:100 to 1:10000. The biggest number of serums had an antibody titer of 1:300, then 1:100, and 1:1000. The smallest number of serums had an antibody titer of 1:10000. Horses seropositive to leptospirosis were established only in sporadic cases, so that no major epizooties were established in the territory of Belgrade.

scholarly journals Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry

2009 ◽  
Vol 63 (1-2) ◽  
pp. 37-44
Author(s):  
Nenad Milic ◽  
Jakov Nisavic ◽  
Marina Radojicic ◽  
Marina Sekler ◽  
Kazimir Matovic ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Blood Serum ◽  
Disease Virus ◽  
Sensitivity And Specificity ◽  
Newcastle Disease ◽  
Hemagglutination Inhibition ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Hemagglutination Inhibition Test ◽  
Serological Diagnostics

Comparative investigations of the sensitivity and specificity of the indirect immunoenzyme probe - iELISA and the hemagglutination inhibition test (HI test) in serological diagnostics of the Newcastle disease in poultry were carried out using samples of blood serum taken from non-vaccinated and vaccinated poultry. A total of 14 samples of blood serum from non-vaccinated poultry were examined using the immunoenzyme probe - iELISA, and nine of these were found to be positive to the presence of specific antigen against the Newcastle disease virus, while two samples were suspect, and no presence of specific antibodies was established in three samples. Examinations of 82 samples of blood serum from vaccinated poultry for the presence of specific antibodies against the Newcastle disease virus established their presence in 80 serum samples, while one sample was suspect and one sample was negative. The values of the titer of specific antibodies in blood serum samples of vaccinated and non-vaccinated poultry established using the hemagglutination inhibition test (HI test) ranged from 1:2 to 1:32.

scholarly journals Examining the presence of specific antibodies against Salmonella enteritidis in vaccinated and unvaccinated poultry

2016 ◽  
Vol 70 (1-2) ◽  
pp. 3-12
Author(s):  
Marina Radojicic ◽  
Maja Markovic ◽  
Jakov Nisavic ◽  
Dejan Krnjaic ◽  
Nemanja Zdravkovic
Keyword(s):  
Blood Serum ◽  
Salmonella Enterica ◽  
Salmonella Enteritidis ◽  
Diagnostic Methods ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Kappa Test ◽  
Agglutination Method ◽  
Serum Testing ◽  
Serological Diagnostics

Introduction. The objective of our research was to point to the significance of serological diagnostic methods, that is, competitive immunoenzyme test - cELISA as well as the method of classical agglutination of O and H salmonella antigen, for specific antibodies against Salmonella enterica subspecies enterica serovar Enteritidis presence and titre detection in blood serum samples of bothvaccinated and unvaccinated poultry. Material and methods. In our work, we have used commercial competitive immunoenzyme test - cELISA and classical agglutination method with O and H antigens of Salmonella enterica subspecies enterica serovar Enteritidis. Comparative testing included 177 blood serum samples of poultry, out of which 137 was from unvaccinated and 40 from vaccinated individuals originating from majority of poutry farms. Results. In 74 blood serum samples, that is 54,01% out of the total of tested samples originating from unvaccinated flocks, by the use of cELISA test, there were found specific antibodies against S. Enteritidis, while by the method of classical agglutination specific antibodies against O antigen 1,9 and 12 were found in 58 samples, that is in 42,34% , and specific antibodies against H antigen g and m were found in 61 samples,what was 44,53% . In all the tested blood serum samples of vaccinated poultry, there was determined the presence of of specific antibodies against S. Enteritidis, both by the use of competitive cELISA method and classical agglutination with somatic O and flagellar H antigens. By the statistical analysis of the results obtained by the method of classical agglutination and cELISA use of kappa test, there was found out a very good compliance (kappa=0,813). Conclusion. Based on the compared results of blood serum testing on the presence of specific antibodies against S. Enteritidis, it can be concluded that cELISA and classic agglutination with O and H antigen methods have a significant place in serological diagnostics of poultry samonelosis, because their application enables detection of the titre of specific antibodies against S. Enteritidis presence in the population of unvaccinated poultry sensitized with antigens of the mentioned causative agent, as well as in unvaccinated animals.

scholarly journals Establishing presence of antibodies against bovine respiratory syncytial virus (BRSV), parainfluenza virus 3 (PI3) and bovine herpesvirus 1 (BHV 1) in blood serum of cattle using indirect immunoenzyme probe

2009 ◽  
Vol 63 (3-4) ◽  
pp. 145-152 ◽  
Author(s):  
Horea Samanc ◽  
Nenad Milic ◽  
Velibor Stojic ◽  
Dejan Knezevic ◽  
Ivan Vujanac ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Blood Serum ◽  
Bovine Herpesvirus ◽  
Bovine Respiratory Syncytial Virus ◽  
Parainfluenza Virus ◽  
Serum Samples ◽  
Bovine Herpesvirus 1 ◽  
Specific Antibodies ◽  
Herpesvirus 1 ◽  
Syncytial Virus

A total of 92 samples of bovine blood serum were examined for the presence of antibodies against the bovine respiratory syncytial virus using indirect immunoenzyme probe - iELISA. Specific antibodies against the bovine respiratory syncytial virus (BRSV) were established in 46, or 50% blood serum samples. Investigations of the 92 blood serum samples of cattle for the presence of antibodies against the parainfluenza virus 3 (PI 3), revealed their presence in 77, or 83.69% of the samples, and the presence of antibodies against the bovine herpesvirus 1 (BHV 1) was established in 19, or 20.65% of the samples.

scholarly journals Comparison seroprevalence of Salmonella spp. in large farms and individual producers in Serbia

2014 ◽  
Vol 30 (1) ◽  
pp. 137-143
Author(s):  
N. Stojanac ◽  
O. Stevancevic ◽  
B. Savic ◽  
I. Stancic ◽  
A. Potkonjak
Keyword(s):  
Blood Serum ◽  
Serum Samples ◽  
Salmonella Spp ◽  
Serological Tests ◽  
Study Objective ◽  
Food Borne ◽  
The Individual ◽  
Large Farms ◽  
Fattening Pigs ◽  
Slaughtered Pigs

Salmonella is one of the most frequently reported food-borne (alimentary) infections in the world. The study objective was to evaluate seroprevalence of Salmonella spp. in the farrow-to-finish farms and individual producers. Examined fattened pigs were divided into two groups: the first group was comprised of fatteners from 4 large farms in northern Serbia, and fatteners from the other group originating from individual producers from northern Serbia and the region of eastern Serbia. Individual blood serum samples were collected from 100 pigs per farrow-to-finish farms and 300 fattening pigs from individual producers and analysed for the presence of Salmonella antibodies. A blood serum sample from each pig was frozen, and blood serum was examined for specific antibodies against Salmonella spp. using an indirect ELISA. Salmonella seroprevalence ranging from 0% to 56% was found in 4 farrow-to-finish farms. Seroprevalence of 79% was found in individual producers (300 blood serum samples). This study shows that the results of serological tests for Salmonella were different (p<0.01) for slaughtered pigs from farms and from individual producers. Pig production in Serbia is under better supervision on large farrow-to-finish farms than in the individual sector. This study is an introduction to reducing of public health risks associated with Salmonella in pork.

scholarly journals The significance of fertile pigs vaccination against porcine parvovirus infection in the prevention of intrauterine infection and formation of colostrum piglets immunity

2014 ◽  
Vol 68 (1-2) ◽  
pp. 55-65
Author(s):  
Nenad Stojanac ◽  
Mladen Gagrcin ◽  
Ognjen Stevancevic ◽  
Ivan Stancic
Keyword(s):  
Blood Serum ◽  
Antibody Titer ◽  
Intrauterine Infection ◽  
Porcine Parvovirus ◽  
Secondary Immune Response ◽  
Specific Antibodies ◽  
Newborn Piglets ◽  
Efficient Measure ◽  
To Come ◽  
Average Body

The aim of this study was to come closer to the knowledge of piglets protection during intrauterine life, as well as formation of colostrum immunity against Porcine Parvovirus Infection (PPV), on the basis of detailed antibody titer analysis from the blood serum of pigs 7 days before previous weaning, 30 days before mating and their piglets during first 3 days of life. The research included 60 fertile pigs and 300 of their offspring. For that purpose we have examined antibody titer specific for PPV in blood serum of vaccinated fertile pigs on 70th and 113th day of gestation, and in the blood serum of piglets originated from itemized fertile pigs during first day of life, before colostrums consummation and also during 3rd day of life. On the 70th day of gestation, in the fertile pigs blood serum, average antibody titer specific for PPV, value of 12.60 was determined, what represents adequate level for solid protection against PPV infection. This was confirmed undoubtedly by examination results of antibodies in the blood serum of piglets before colostrum consummation, which was 100% negative. Titer drop in the blood serum of fertile pigs, on 113th day of gestation (on the level of 8.7) came as a result of specific antibodies transfer from the mother?s blood flow to the colostrum. The above-mentioned is supported by the fact that on the 3rd day of life there was confirmed high average body titer level (13.37) in newborn piglets body serum has been confirmed. The principle of fertile pigs vaccination 7 days before weaning and one month before gilts insemination is an efficient measure which prevents intrauterine infection occurrence during the entire gestation process. The level of antibodies specific for PPV which is determined in gilts blood serum after vaccination can be a result of both primary and secondary immune response (animal infected before first vaccination), as well as absence of gilts revaccination, what is usually recommended by producers, may not always be regarded as a failure.

scholarly journals Investigations of presence of antibodies against bovine herpesvirus-1 in blood serum of calves prior to colostrum diet

2010 ◽  
Vol 64 (1-2) ◽  
pp. 33-41
Author(s):  
Sava Lazic ◽  
Dragan Rogan ◽  
Tamas Petrovic ◽  
Dejan Bugarski ◽  
Diana Lupulovic ◽  
...  
Keyword(s):  
Blood Serum ◽  
Antibody Titer ◽  
Bovine Herpesvirus ◽  
Virus Neutralization ◽  
Serum Samples ◽  
Bovine Herpesvirus 1 ◽  
Breeding Material ◽  
Mdbk Cells ◽  
Herpesvirus 1

The paper presents the results of investigations of the presence of the bovine herpesvirus-1 (BHV-1) in samples of blood serum from 106 cows and 107 of their calves (one cow had twins). Blood was sampled from the cows immediately after parturition, and from the calves before feeding on colostrum. The examined cows and their calves originated from 5 herds in which previous investigations had shown infection with the bovine herpesvirus-1. The determination of antibodies against BHV-1 was performed using the method of virus neutralization in culture of MDBK cells with 100 TCID/50 viruses (BHV-1, TN-41 Am. Bio Research, USA). Antibodies against BHV-1 were determined in all blood serum samples of cows and in 16 samples of precolostral blood serums of calves. The antibody titer values in cows ranged from 1:4 to 1:512, and in calves the determined values were from 1:2 to 1:16. The results indicate that cows that are seropositive to BHV-1 can deliver calves seropositive to BHV-1 in about 15% cases. This must be kept in mind in selecting cows for the production of breeding material, in particular bulls for reproduction centers, as well as in making a programme for the immunoprophylaxis of calves against BHV-1. .

scholarly journals COMPARATIVE INVESTIGATION OF DIFFERENT METHODS FOR THE DETECTION OF INFECTION IN RABBITS CHALLENGED WITH L. interrogans SEROTYPE hardjo

2014 ◽  
Vol 6 (2) ◽  
pp. 71-85
Author(s):  
Živoslav Grgić ◽  
Bosiljka Đuričić ◽  
Branka Vidić ◽  
Sara Savić ◽  
Ivan Pušić
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Serum ◽  
Blood Sample ◽  
Positive Finding ◽  
Post Challenge ◽  
Chain Reaction ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Pcr Method ◽  
Polymerase Chain

Keeping up-to-date with modern diagnostic techniques for leptospirosis as well as continuous improvement of laboratory diagnostic methods resulted in abundant knowledge on the nature and consequences of this infection and its importance in both human and veterinary medicine. In that respect, development and introduction of novel diagnostic tests and procedures have become the paramount issue in the diagnostics of leptospirosis and related infectious diseases. Thus, the goal of this research was to investigate the application of diverse laboratory methods and to evaluate their validity in the diagnostics of leptospirosis. Eleven rabbits were artificially infected with live cultures of L. interrogans serovar hardjo by the method of skin scarification. Blood and blood serum samples of challenged animals were collected every other day throughout the 3-week period (i.e. until day 21), and then once weekly during following five weeks. Blood sera were tested for the presence of L. interrogans serovar hardjo specific antibodies applying the methods of microscopic agglutination (MA) test and ELISA. Blood samples were examined using the method of cultivation in liquid medium by Johnson supplemented with 200μg/1ml 5- fluorouracil (5-FU). Presence/absence of L.interrogans serovar hardjo was confirmed by polymerase chain reaction (PCR) method. In this reaction, a pair of primers separated from the basic structure of the Leptospira interrogans rrs (16S) gene. In MA test, the presence of specific antibodies against L. hardjo in rabbits was confirmed in 67 (36.61%) of 183 investigated sera. Initial positive specific antibody finding was recorded on day 9 post challenge, and it persisted until day 17. In ELISA test, positive and suspect findings were confirmed in 67 and 18 samples, respectively. Initial ELISA-positive finding was observed on day 15, showing increasing tendency throughout the monitoring period and reaching its maximum value on day 42. Method of blood sample cultivation resulted in isolation of L. interrogans serovar hardjo in 33 (18.03%) on day 3 at the earliest, whilst highest isolation rate was observed on day 17 post challenge. Applying polymerase chain reaction (PCR) method, genome or genome sequences of L. interrogans serovar hardjo were detected in 67 (56.30%) out of 119 blood serum samples. PCR method revealed positive finding as early as on day 1 post challenge, whereas the highest rate of positive findings was recorded on day 19. Comparison of the results obtained by methods of cultivation and PCR during the period from experimental day 1 to 21, i.e. period prior to administration of chemotherapeutic agents, demonstrated high level of linear correlation of r = 0.8105 at the 0.01 significance level. After dihydrostreptomycin therapy administered from day 21 post infection, L. interrogans serovar hardjo could not be isolated using the method of blood sample cultivation. Contrary to that, PCR method revealed the presence of L. interrogans serovar hardjo genome in 23 samples.

scholarly journals Screening of SRLV in herds in mid-Eastern Poland

2018 ◽  
Vol 74 (8) ◽  
pp. 532-535
Author(s):  
WIKTOR BOJAR ◽  
ANDRZEJ JUNKUSZEW ◽  
MONIKA OLECH ◽  
JACEK KUŹMAK ◽  
KLAUDIUSZ SZCZEPANIAK
Keyword(s):  
Blood Serum ◽  
Detailed Analysis ◽  
Elisa Test ◽  
Serological Response ◽  
Flock Size ◽  
Serological Analysis ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Merino Sheep ◽  
Detailed Diagnosis

The aim of the study was to evaluate the epidemiological condition of SRLV infections in nucleus flocks of sheep. The surveillance of Lentivirus infections was conducted in sheep flocks from the mid-Eastern part of Poland: the area of Lubelskie, Świętokrzyskie and Podkarpacie provinces. Ninety eight flocks were chosen for a detailed analysis. They included 6,470 mother ewes belonging to 15 breeds and lines: Polish Merino sheep, Polish Lowland sheep, Wielkopolska sheep, Żelaźnieńska sheep, Uhruska sheep, Olkuska sheep, Polish Heath sheep, Świniarka sheep, Polish Pogorze sheep, Podhale Zackel sheep, Black-headed sheep, Ile de France, Berrichon du Cher, BCP and SCP. The identification of the infected animals and the assessment of the epidemiological condition of the flocks was carried out using a serological analysis of blood serum samples. The assay of MVV-specific antibodies in blood serum was performed using the ELISA test. The results obtained showed that the highest rate of flocks with at least one animal with a positive serological response was in the Podkarpacie Province (71.43%), and the lowest rate (27.03%) was observed in Lubelskie Province. The analysis of the rate of the sheep infected with SRLV indicated that the greatest number of seropositive animals was highest in Podkarpackie Province (19.9%), which corresponds to the results for the flocks, and was significantly higher (p ≤ 0.01) than in Świętokrzyskie Province (11.35%) and Lubelskie Province (9.20%). The flock size turned out to be a factor which significantly influenced the prevalence of SRLV infections. The prevalence was highest (13.6%) in the flocks exceeding 100 sheep, and decreased together with the decreasing number of heads in the flock. It must be stressed that the problem of SRLV infections is very complex and depends on many factors. Therefore it is necessary to carry out a detailed diagnosis of the factors leading to expansion of the virus in sheep flocks before developing flock protection programs...

scholarly journals Production and Evaluation of Immunoglobulin Y Anti-Brucella abortus (Vaccinal Strain B19)

2018 ◽  
Vol 46 (1) ◽  
pp. 8
Author(s):  
Pollyanna Mafra Soares ◽  
Mayara Mafra Soares ◽  
Mariane Pacheco dos Santos Lourencetti ◽  
Muriell Ribeiro Ganda ◽  
Mariana Assunção de Souza ◽  
...  
Keyword(s):  
Blood Serum ◽  
Brucella Abortus ◽  
Diagnostic Tests ◽  
High Specificity ◽  
Egg Yolk ◽  
Control Group ◽  
Immunoglobulin Y ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Group 2

Background: The immunoglobulin Y is a principal antibody current in the blood of hens, which are transferred from the maternal blood serum to the egg yolk. The extraction of IgY from the egg yolk apply animal welfare when compared to the extraction of IgG, reducing the number of animals and prevent a bleeding of hens through the extraction of the IgY from eggs, besides that IgY presenting high specificity for antigenic binding. The objective of this study was to produce specific polyclonal IgY antibodies anti-Brucella abortus by immunizing hens with B19 vaccine and evaluate their reactivity through Buffered Acidified Plate Antigen (BAPA), 2-Mercaptoethanol (2-ME) and indirect ELISA diagnostic tests.Materials, Methods & Results: Four 25-week-old White Leghorn hens were immunized, two of them comprising the control group (Group 1) with phosphate-buffered saline (PBS) with adjuvant, and the others two immunized with B19 vaccine (Brucella abortus vaccine strain B19), representing the Group 2. The immunizations occurred six times with a 15-day interval between each. Blood samples were taken biweekly (seven times); and daily, the eggs were collected for 13 weeks, the first collection of blood and eggs, performed one week before the first immunization of each group. The IgY was purified from egg yolk, using the method of dilution in acid water and precipitation with ammonium sulfate for delipidation. BAPA, 2-ME and ELISA tests performed to verify the specificity of IgY confirmed the reactivity of polyclonal antibodies specific to the antigen used both in blood serum samples and in the purified egg yolks. The hens from the control group did not present reactivity in the diagnostic tests used, which was already expected, since no antigen was used in any of their immunizations. Hens immunized with the Brucella abortus B19 vaccine produced detectable reactive antibodies in the three tests used on blood serum and IgY samples extracted from the egg yolk. In Group 2 (vaccine B19), blood serum samples started to react one week after the first inoculation, and the IgY samples extracted from the egg yolk were reagent two weeks after serum IgY appear reactivity, showing the transfer of specific antibodies to the egg yolk, was late.Discussion: Although the transfer of serum Igy to egg yolk was late when compared to others authors which found that the transovarian passage of immunoglobulin Y occurred in approximately three to six days after IgY being detected in blood serum, the results of this study showed the occurrence of the transfer of blood serum IgY anti-Brucella abortus to egg yolk of hens immunized with B19 vaccine, the same found by others researches found the same results with others antigens. Thus, it can be concluded that immunoglobulins Y produced in this study can be used as specific antibodies in diagnostic tests for the detection of the Brucella abortus antigen, in addition, this process guarantees the welfare of the animal, since it avoids bleeding and it is possible to obtain high concentrations of antibodies directly from the hen egg, which is a great advantage, because IgY can be easily isolated from the egg yolk by the precipitation technique discarding the need of invasive and painful procedures that involve bloody interventions to obtain the serum antibodies like occur in mammals for extraction of IgG.

scholarly journals A screening for serum markers of arbovirus infections in healthy blood donors from the Volgograd Region

2020 ◽  
Vol 9 (5-6) ◽  
pp. 743-749
Author(s):  
A. О. Negodenkoa ◽  
D. N. Luchinin ◽  
P. Sh. Konovalov ◽  
O. A. Pavlyukova ◽  
E. A. Skrynnikova ◽  
...  
Keyword(s):  
Blood Serum ◽  
Blood Donors ◽  
Clinical Signs ◽  
Serum Samples ◽  
West Nile ◽  
Specific Antibodies ◽  
Age Related ◽  
Tick Borne Encephalitis ◽  
Volgograd Region ◽  
California Serogroup

Volgograd region is considered to be endemic West Nile fever (WNF) area due to the established circulation of the West Nile virus (WNV) therein. Some previous independent studies examining samples collected on the territory of the Volgograd region revealed markers related to the California serogroup (CSG), Sindbis and Ukuniemi viruses. WNF, CSG, Sindbis and Ukuniemi fever mainly being asymptomatic posing thereby a threat of virus spread due to transfusiological manipulations along with vector-borne transmission are manifested by diverse clinical signs. The study was aimed at detection of antibodies specific to West Nile, tick-borne encephalitis, California serogroup (Tyaginya, Inko), Sidbis, and Ukuniemi viruses in blood donors from the Volgograd region. For this, 404 blood sera samples collected from blood donors residing in the Volgograd as well as the Volgograd region were examined by ELISA. It was found that percentage of blood serum samples positive for arbovirus-specific antibodies was 18.32%. Among the, 67 out of 404 (16.58%) samples contained anti-WNV antibodies, 3 out of 279 (1.08%) samples — to tick-borne encephalitis virus, 1 out of 92 (1.09%) — to California serogroup fever and Ukuniemi viruses, and 2 out of 92 (2.17%) — to Sindbis fever virus. Importantly, the peak number of IgG and IgM WNV-positive samples was found among residents of Volgograd (29 out of 110, 26%) and Oktyabrsky district (7 out of 25, 28%). In addition, anti-Sindbis, Ukuniemi and CSG virus antibodies were detected in blood serum samples from blood donors residing in the Kalachevsky region and the city of Volgograd. Analyzing age-related distribution and percentage of seropositive subjects in each age group showed as follows: the lowest percentage (14.5%) was positive for anti-WNV, Sindbis, Ukuniemi and CSG virus antibodies in blood donors aged 32–41 years, whereas the peak percentage (25%) — in the subjects aged 52–61 years. Thus, virus specific antibodies detected in healthy individuals in the aforementioned region evidence about potential recovery after asymptomatic infections. In this regard, further research is required to determine a role of the such arboviruses in the structure of regional infectious diseases. The data obtained suggest a need to continue serological arbovirus monitoring in the Volgograd region.

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