scholarly journals Influence of uremia and peritoneal dialysis on mesothelial cells of the peritoneum

2002 ◽  
Vol 59 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Dusan Trpinac ◽  
Biljana Stojimirovic ◽  
Miljana Obradovic ◽  
Drago Milutinovic ◽  
Dragan Obradovic ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Renal Failure ◽  
Peritoneal Dialysis ◽  
Basal Membrane ◽  
Mesothelial Cells ◽  
Endoplasmatic Reticulum ◽  
Terminal Renal Failure ◽  
Transmission Electron ◽  
Different Shapes

The aim of the study was to investigate the morphology of mesothelial cells of the peritoneum of patients with terminal renal failure (TRF), taken by the biopsy immediately before the onset of peritoneal dialysis (PD), and to compare it with the findings in patients with PD. The samples were prepared in the way standard for light microscopy and transmission electron microscopy. In patients with TRF intracytoplasmatic inclusions could be observed, unusual protrusions of mesothelial apical surfaces, deformation of mesothelial cells and their detachment from the basal membrane, as well as the dilatated cisternae of granulated endoplasmatic reticulum with filamentous structures in some of them. In patients on PD cytoplasmic protrusions of different shapes and contents were observed at the surface of mesothelial cells, multiplication of basal membrane, occurrence of young forms of mesothelial cells as well as the detachment of those cells from the basal lamina.

The effect of praziquantel on the ultrastructure of Schistosoma margrebowiei

1991 ◽  
Vol 65 (2) ◽  
pp. 79-88 ◽  
Author(s):  
A. H. H. Awad ◽  
A. J. Probert
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Immune System ◽  
Neck Region ◽  
Basal Membrane ◽  
Host Cells ◽  
Time Intervals ◽  
Entire Surface ◽  
Transmission Electron ◽  
Severe Erosion

ABSTRACTThe effect of various concentrations of praziquantel at different time intervals post-treatment on the ultrastructure of Schistosoma margrebowiei using scanning and transmission electron microscopy has been examined. The major changes involved blebbing of the entire surface tegument of both sexes (although more marked in males) together with vacuolation of the basal membrane accompanied by the development of membraneous whorls. These effects were progressively more marked with increased concentration and time of exposure resulting in severe erosion of the tubercles and collapse of the sensory organelles. Exposure of the underlying tegumental tissue resulted and paralysis and contraction of the suckers and neck region was apparent. Disruption of the subtegumental musculature and the appearance of vacuolation and membraneous whorl formation were seen. The gastrodermis was similarly affected and the S4 cells of the vitelline gland showed protein disruption of the vitelline droplets. Host cells were seen adhering to the surface of the worms following drug treatment and the synergism between PZQ and the action of the hosts immune system has been discussed.

Topography and Ultrastructure of the tegument of adultSchistosoma mekongi

Parasitology ◽  
1984 ◽  
Vol 89 (3) ◽  
pp. 511-521 ◽  
Author(s):  
P. Sobhon ◽  
E. S. Upatham ◽  
Diane J. McLaren
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Outer Membrane ◽  
Inclusion Bodies ◽  
Unique Feature ◽  
Basal Membrane ◽  
The Body ◽  
Transmission Electron ◽  
Schistosoma Mekongi ◽  
Membrane Infoldings

SUMMARYThe tegument of adultSchistosoma mekongihas been studied by both scanning and transmission electron microscopy. The gross surface topography of the parasite resembles that ofS. japonicum, in that branched ridges, microvilli and sensory papillae predominate; such characteristics distinguish these two species from the non-oriental schistosomes. A unique feature ofS. mekongi, however, is the numerous pleomorphic protruberances which are concentrated particularly on the middle three-fourths of the body surface. Transmission electron microscopy has revealed that these protruberances enclose bundles of microfilaments which appear to insert into the tegumental outer membrane. The microfilaments are suggested to have a supportive or stabilizing function, and may compensate for the absence of more typical crystalline spines. The tegumental outer membrane is typically heptalaminate in section, while the basal membrane infoldings are surrounded by concentrations of mitochondria. Three types of tegumental inclusion bodies have been recognized. Discoid bodies and membraneous bodies are morphologically identical to those described in all other schistosome species, except that the latter inclusions have been seen connected to each other and to the tegumental outer membrane by unique channels lined with trilaminate membrane. The third inclusion takes the form of spherical, lucent vesicles containing membrane fragments; these may represent the remains of spent membraneous bodies.

The morphology of the attachment and probable feeding site of the nematode Trichuris muris (Schrank, 1788) Hall, 1916

1978 ◽  
Vol 56 (9) ◽  
pp. 1889-1905 ◽  
Author(s):  
T. D. G. Lee ◽  
K. A. Wright
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Membrane ◽  
Lipid Droplets ◽  
Syncytium Formation ◽  
Basal Membrane ◽  
Trichuris Muris ◽  
Feeding Site ◽  
Transmission Electron ◽  
Epithelial Sheet

The 'tunnel' within which the nematode Trichuris muris is contained was examined by light, scanning, and transmission electron microscopy. The amount of worm covered by the tunnel varied with age. Young larval worms were completely embedded in the host's intestinal mucosa whereas older larvae and adults had part, if not all, of the posterior region protruding into the lumen. All worms were found to have heads embedded in the tissue and in no cases were whole worms found free in the lumen.The 'tunnel' was shown to be a syncytial protoplasmic mass with recognizable cellular elements such as nuclei, lipid droplets, mitrochondria, and mucous droplets anteriorly whereas more distal to the head these elements became increasingly scarce and degenerated. The syncytium is bordered apically, laterally, and basally by cell membrane. The basal lamina can be identified beneath the basal membrane of the syncytium indicating that syncytium formation occurs in the epithelial sheet only and does not extend into the lamina propria. Evidence suggests that the nematode initially induces a syncytium about its head, feeds on the syncytial cytoplasm, and then moves on to initiate extension of the syncytium. The result of this movement is a 'tunnel' snaking across the caecum and colon.

scholarly journals Ultrastructure of the tegument of Metamicrocotyla macracantha (Alexander, 1954) Koratha, 1955 (Monogenea, Microcotylidae)

2004 ◽  
Vol 64 (1) ◽  
pp. 27-31 ◽  
Author(s):  
S. C. Cohen ◽  
A. Kohn ◽  
M. F. D. Baptista-Farias
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Muscle Fibers ◽  
Circular Muscle ◽  
The Body ◽  
Endoplasmatic Reticulum ◽  
Transmission Electron

The ultrastructure of the body tegument of Metamicrocotyla macracantha (Alexander, 1954) Koratha, 1955, parasite of Mugil liza from Brazil, was studied by transmission electron microscopy. The body tegument is composed of an external syncytial layer, musculature, and an inner layer containing tegumental cells. The syncytium consists of a matrix containing three types of body inclusions and mitochondria. The musculature is constituted of several layers of longitudinal and circular muscle fibers. The tegumental cells present a well-developed nucleus, cytoplasm filled with ribosomes, rough endoplasmatic reticulum and mitochondria, and characteristic organelles of tegumental cells.

Identification of Shape Transitions in Coherent Ge/Si Islands Using Transmission Electron Microscopy

1999 ◽  
Vol 583 ◽  
Author(s):  
Chuan-Pu Liu ◽  
William L. Henstrom ◽  
David G. Cahill ◽  
J. Murray Gibson
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Strain Relaxation ◽  
Large Population ◽  
Shape Transition ◽  
Plan View ◽  
First Order ◽  
Transmission Electron ◽  
Metastable Form ◽  
Different Shapes

AbstractAs a consequence of strain relaxation, Ge coherent islands on Si(001) substrates evolve to different shapes as islands grow. By measuring the size and the strain simultaneously in a large population of individual islands using two simple and robust plan-view transmission electron microscopy-based techniques, we can identify island shapes easily because island shape is a function of strain. We briefly introduce the mechanisms of these two techniques. We then show that there is a metastable shape of Ge islands involved in the shape transition between pyramids and domes. The strain relaxation changes discontinuously as islands grow from pyramids to the metastable form and then finally to domes indicating that the shape transition between pyramids and domes is first order. We also show that the shape of this metastable island is a truncated dome and the faceted planes are {103}.

Techniques for Transmission Electron Microscopy of Fiber-Matrix Interfaces in Aluminum Alloy-Boron Composites by Ion Erosio

1971 ◽  
Vol 29 ◽  
pp. 204-205
Author(s):  
G. G. Shaw
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Aluminum Alloy ◽  
Electron Beam ◽  
Pure Aluminum ◽  
Ion Milling ◽  
Transmission Electron ◽  
Fiber Matrix ◽  
Ion Erosion ◽  
Row Of Fibers

The morphology and composition of the fiber-matrix interface can best be studied by transmission electron microscopy and electron diffraction. For some composites satisfactory samples can be prepared by electropolishing. For others such as aluminum alloy-boron composites ion erosion is necessary.When one wishes to examine a specimen with the electron beam perpendicular to the fiber, preparation is as follows: A 1/8 in. disk is cut from the sample with a cylindrical tool by spark machining. Thin slices, 5 mils thick, containing one row of fibers, are then, spark-machined from the disk. After spark machining, the slice is carefully polished with diamond paste until the row of fibers is exposed on each side, as shown in Figure 1.In the case where examination is desired with the electron beam parallel to the fiber, preparation is as follows: Experimental composites are usually 50 mils or less in thickness so an auxiliary holder is necessary during ion milling and for easy transfer to the electron microscope. This holder is pure aluminum sheet, 3 mils thick.

Direct Observation of Heat Exchanger Deposits by Cross Sectioning

1967 ◽  
Vol 25 ◽  
pp. 364-365
Author(s):  
R. W. Anderson ◽  
D. L. Senecal
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Heat Exchanger ◽  
Direct Observation ◽  
Cross Sections ◽  
Preparation Method ◽  
Specimen Preparation ◽  
Flowing Water ◽  
Transmission Electron ◽  
Deposit Layer

A problem was presented to observe the packing densities of deposits of sub-micron corrosion product particles. The deposits were 5-100 mils thick and had formed on the inside surfaces of 3/8 inch diameter Zircaloy-2 heat exchanger tubes. The particles were iron oxides deposited from flowing water and consequently were only weakly bonded. Particular care was required during handling to preserve the original formations of the deposits. The specimen preparation method described below allowed direct observation of cross sections of the deposit layers by transmission electron microscopy.The specimens were short sections of the tubes (about 3 inches long) that were carefully cut from the systems. The insides of the tube sections were first coated with a thin layer of a fluid epoxy resin by dipping. This coating served to impregnate the deposit layer as well as to protect the layer if subsequent handling were required.

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