scholarly journals Microbiological transformations of phosphorus and sulphur compounds in acid soils

2012 ◽  
pp. 27-36
Author(s):  
Dragana Stamenov ◽  
Mirjana Jarak ◽  
Simonida Djuric ◽  
Hajnal Jafari ◽  
Dragana Bjelic
Keyword(s):  
Organic Phosphorus ◽  
Humus Content ◽  
Acid Soils ◽  
Phosphorus Compounds ◽  
Phosphate Solubilizing Bacteria ◽  
Sulphur Compounds ◽  
Organic Sulphur ◽  
Number Of Microorganisms ◽  
Phosphate Solubilizing ◽  
Number Of Bacteria

The dynamics of phosphorus and sulphur in soil is closely related to the dynamics of the biological cycle in which microorganisms play a central role. There is not much microbiological activity in acid soils because aerobes are scarce, rhizosphere is restricted to the shallow surface layer, and the biomass of microorganisms decreases with higher acidity. The aim of the research was to investigate the number of microorganisms, which decompose organic and inorganic phosphorus compounds and organic sulphur compounds in calcocambisol, luvisol, and pseudogley. The following parameters were determined in the soil samples: pH in H2O and in 1MKCl; the content of CaCO3 (%); humus content (%), nitrogen content (%); the content of physiologically active phosphorus and potassium (mg P2O5/100g of soil; mg K2O/100g of soil). The number of microorganisms was determined by the method of agar plates on appropriate nutrient media: the number of microorganisms solubilizing phosphates on a medium by Muramcov; the number of microorganisms that decompose organic phosphorus compounds on a medium with lecithin; and the number of microorganisms that transform organic sulphur compounds on a medium by Baar. All three types of soil are acid non-carbonate soils with a low level of available phosphorus and a more favorable amount of potassium, nitrogen, and humus. The largest number of bacteria, which transform organic phosphorus compounds, was found in calcocambisol. The largest number of phosphate solubilizing bacteria was recorded in pseudogley, whereas the largest number of phosphate solubilizing fungi was recorded in calcocambisol. The largest number of bacteria, which transform organic sulphur compounds, was recorded in pseudogley.

scholarly journals Secretion of Gluconic Acid From Nguyenibacter sp. L1 Is Responsible for Solubilization of Aluminum Phosphate

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiao Li Li ◽  
Xue Qiang Zhao ◽  
Xiao Ying Dong ◽  
Jian Feng Ma ◽  
Ren Fang Shen
Keyword(s):  
Gluconic Acid ◽  
Calcareous Soils ◽  
Acid Soils ◽  
Aluminum Phosphate ◽  
Available P ◽  
Rrna Gene ◽  
Phosphate Solubilizing Bacteria ◽  
P Deficiency ◽  
Phosphate Solubilizing ◽  
Major Factors

Phosphorus (P) deficiency is one of the major factors limiting plant growth in acid soils, where most P is fixed by toxic aluminum (Al). Phosphate-solubilizing bacteria (PSBs) are important for the solubilization of fixed P in soils. Many PSBs have been isolated from neutral and calcareous soils, where calcium phosphate is the main P form, whereas PSBs in acid soils have received relatively little attention. In this study, we isolated a PSB strain from the rhizosphere of Lespedeza bicolor, a plant well adapted to acid soils. On the basis of its 16S rRNA gene sequence, this strain was identified as a Nguyenibacter species and named L1. After incubation of Nguyenibacter sp. L1 for 48 h in a culture medium containing AlPO4 as the sole P source, the concentration of available P increased from 10 to 225 mg L–1, and the pH decreased from 5.5 to 2.5. Nguyenibacter sp. L1 exhibited poor FePO4 solubilization ability. When the pH of non-PSB-inoculated medium was manually adjusted from 5.5 to 2.5, the concentration of available P only increased from 6 to 65 mg L–1, which indicates that growth medium acidification was not the main contributor to the solubilization of AlPO4 by Nguyenibacter sp. L1. In the presence of glucose, but not fructose, Nguyenibacter sp. L1 released large amounts of gluconic acid to solubilize AlPO4. Furthermore, external addition of gluconic acid enhanced AlPO4 solubilization and reduced Al toxicity to plants. We conclude that secretion of gluconic acid by Nguyenibacter sp. L1, which is dependent on glucose supply, is responsible for AlPO4 solubilization as well as the alleviation of Al phytotoxicity by this bacterial strain.

scholarly journals Isolasi Bakteri Pelarut Fosfat Genus Pseudomonas dari Tanah Masam Bekas Areal Perkebunan Karet di Kawasan Institut Teknologi Sumatera

2020 ◽  
Vol 21 (1) ◽  
pp. 40-48
Author(s):  
Muhammad Asril ◽  
Yuni Lisafitri
Keyword(s):  
Soil Microbes ◽  
Acid Soil ◽  
Acid Soils ◽  
Soil Samples ◽  
Pathogenicity Test ◽  
P Availability ◽  
Phosphate Solubilizing Bacteria ◽  
Potato Tubers ◽  
Phosphate Availability ◽  
Phosphate Solubilizing

ABSTRACTPhosphorus (P) is a nutrient that is needed by plants. The availability of this element is greatly influenced by soil pH. As for ultisol soils classified as acid soils, most of the P in the soil is not available and is bound to Fe and Al. Pseudomonas, a phosphate solubilizing bacteria are soil microbes that can improve the availability of P in acid soils. This study aims to obtain Pseudomonas indigenous, a phosphate solubilizing bacteria from the acid soil formerly used by rubber plantations in the Institut Teknologi Sumatera. The study was conducted from April to June 2018 which included soil chemical analysis, isolation of the genus Pseudomonads on specific media, testing of phosphate solubility on solid Pikovskaya medium and simple pathogenicity test on potato tubers. The results showed that the sample soil was acidic with a pH of 4.09 with a P-availability of 0.78 ppm. From the soil samples, four potential isolates were obtained from the genus Pseudomonas, namely GSP 01, GSP 13, GSP 15 and GSP 06, with phosphate solubility indexes of 0.885, 0.639, 0.619 and 0.568, respectively. Isolates have the best phosphate solubilizing index on days 4 through 7. The four potential isolates are not pathogenic, so they can be used as isolates to improve the availability of soil nutrients, especially phosphorus needed by plants.Keywords: acid soil, phosphate solubilizing bacteria, phosphate availability, PseudomonasABSTRAKFosfor (P) merupakan unsur hara yang sangat dibutuhkan oleh tanaman. Ketersediaan unsur ini sangat dipengaruhi oleh pH tanah. Pada jenis tanah ultisol yang digolongkan sebagai tanah masam, sebagian besar dari P di tanah dalam bentuk yang tidak tersedia untuk diserap oleh tanaman dan berikatan dengan Fe dan Al. Pseudomonas pelarut fosfat merupakan mikroba tanah yang dapat memperbaiki ketersediaan P pada tanah masam. Penelitian ini bertujuan untuk mendapatkan Pseudomonas pelarut fosfat indigenous dari tanah masam bekas lahan perkebunan karet di kawasan Institut Teknologi Sumatera. Penelitian dilaksanakan pada bulan April sampai Juni 2018 yang meliputi analisis kimia tanah, isolasi bakteri genus Pseudomonads pada medium spesifik, uji kemampuan pelarutan fosfat pada medium Pikovskaya padat serta uji patogenitas sederhana pada umbi kentang. Hasil penelitian menunjukkan bahwa tanah sampel bersifat masam dengan pH 4,09 dengan P tersedia sebesar 0,78 ppm. Dari sampel tanah diperoleh empat isolat potensial yang diperoleh merupakan genus Pseudomonas yaitu GSP 01, GSP 13, GSP 15 dan GSP 06, dengan indeks pelarutan fosfat berturut-turut sebesar 0,885, 0,639, 0,619 dan 0,568. Isolat memiliki indeks pelarutan fosfat terbaik pada hari ke-4 hingga hari ke-7. Keempat isolat potensial tidak bersifat patogen sehingga mampu dijadikan sebagai isolat yang dapat digunakan untuk memperbaiki ketersediaan unsur hara tanah terutama fosfor yang dibutuhkan oleh tanaman.Kata kunci: bakteri pelarut fosfat, fosfat tersedia, Pseudomonas, tanah masam 

Research on the Preparation of Fir Bacterial Fertilizer Using Biological Material

2012 ◽  
Vol 590 ◽  
pp. 100-105
Author(s):  
De Ming Zhou ◽  
He Li ◽  
Rong Li ◽  
Dan Xue Zhu ◽  
Yi Ming Tan
Keyword(s):  
Enzyme Activity ◽  
Organic Phosphorus ◽  
Fermentation Medium ◽  
Inorganic Phosphorus ◽  
Phosphate Solubilizing Bacteria ◽  
Dissolve Inorganic Phosphorus ◽  
Solubilizing Power ◽  
Dissolve Organic Phosphorus ◽  
Mixed Bacteria ◽  
Phosphate Solubilizing

The azotobacter bacteria and the phosphate-solubilizing bacteria are separated and filtered from the rhizosphere soil of the fir plantation and the enzyme activity of azotobacter bacteria, the solubilizing power of phosphate-solubilizing bacteria and the characteristics of PGPR bacteria to produce IAA are measured in this paper. The results show that: there are 5 of the 16 azotobacter bacteria whose enzyme activity is greater than 150 nmol•mL-1•h-1, respectively NGJ-4, NGX-5, NGX-4, NGX-8 and NGJ-8. Py16, Py10 and Py3 own the strongest capacity to dissolve organic phosphorus, respectively 71.31 mg / L, 59.07 mg of / L and 65.14 mg / L; Pw10，Pw6 and Pw20 own the strongest capacity to dissolve inorganic phosphorus, respectively 232.0 mg/L，185.9 mg/L，172.6 mg/L. Py18，Py16 and Py3 own the strongest capacity to produce IAA and dissolve the organic phosphorus bacteria, respectively 38.80mg / L, and 37.29mg / L, and 35.79mg / L; Pw6, Pw8 and Pw21 own the strongest capacity to produce IAA and dissolve the inorganic phosphorus bacteria, respectively 45.340 mg/L, 39.340 mg/L, 27.480 mg/ L. Based on these results, the strains of NGJ-4, NGX-5 and NGJ-8 are selected to prepare the microbial compound bacterial fertilizer using Py16, Py3 and Pw6. Then the azotobacter bacteria, organic phosphate-solubilizing bacteria and inorganic phosphate-solubilizing bacteria is respectively diluted to the solution with the ratio of 15%, and then mix them with the volume proportion of 1.5:1:1 to obtain the mixed bacteria liquid; the proportion of solid carrier ash is 20%, the proportion of fermentation medium is 50%, and the proportion of the mixed bacteria liquid is 30%.

scholarly journals Efektivitas dolomit teraktivasi yang diperkaya dengan bakteri pelarut fosfat sebagai pengganti kiserit pada bibit kakao The effectiveness of activated dolomite enriched by phosphate solubilizing bacteria as kieserite substitute on cocoa seedling

2016 ◽  
Vol 80 (1) ◽  
Author(s):  
Laksmita Prima SANTI ◽  
Didiek Hadjar GOENADI
Keyword(s):  
Acid Soils ◽  
Dry Mass ◽  
Test Plant ◽  
Phosphate Solubilizing Bacteria ◽  
Pseudomonas Sp ◽  
Npk Fertilizers ◽  
Standard Dosage ◽  
Tropical Regions ◽  
Mg Deficiency ◽  
Phosphate Solubilizing

Abstract Magnesium (Mg) deficiency is commonly found in acid soils with high weathering intensity as widely present in tropical and sub-tropical regions, or those developed from low Mg-content parent materials. To overcome Mg deficiency, dolomite is often used to replace kieserite although the former is less reactive than the later. An activation technology by heating up to 800oC, followed by sulfic acid addition, then enriched by phosphate solubilizing bacteria is prospective to improve Mg solubility in soils. This research was aimed to enhance dolomite reactivity and to determine the effectiveness of activated dolomite enriched by phosphate solubilizing bacteria as Mg nutrient sources substituting kieserite on cocoa seedling growth. The results indicate that total and solubility in water of MgO from the prototype of activated dolomite were 26.7 and 9.2%, respectively. Using cocoa seedling as test plant, the use of activated dolomite enriched by using 109 cfu of Pseudomonas sp. at 3.85 g/seedling and combined with 100% dosages of NPK fertilizers showed the best vegetative growth and total dry mass of seedlings was significantly higher than that of 100% NPK-Mg kieserite standard dosage Abstrak Defisiensi Mg umumnya ditemukan pada tanah masam dengan tingkat pelapukan tinggi yang banyak terdapat di daerah tropik, subtropik, dan tanah yang terbentuk dari bahan induk miskin Mg. Untuk mengatasi defisiensi Mg, dolomit sering digunakan sebagai pengganti kiserit walaupun sifatnya kurang reaktif jika dibandingkan kiserit. Teknologi aktivasi dengan pemanasan mencapai 800oC, dilanjutkan dengan penambahan asam sulfat serta pengkayaan dengan bakteri pelarut fosfat diharapkan dapat meningkatkan kelarutan Mg di dalam tanah. Penelitian ini bertujuan untuk meningkatkan reaktivitas dolomit serta menetapkan efektivitas dolomit teraktivasi yang diperkaya dengan bakteri pelarut fosfat sebagai sumber hara Mg pengganti kieserit pada bibit kakao. Hasil yang diperoleh dari penelitian ini mengindikasikan bahwa kadar MgO total dan kelarutan dalam air pada dolomit teraktivasi masing-masing 26,7 dan 9,2%. Dengan menggunakan bibit kakao sebagai tanaman uji, aplikasi 3,85 g dolomit teraktivasi yang diperkaya dengan 109 cfu Pseudomonas sp./bibit yang dikombinasikan dengan 100% dosis pupuk NPK menunjukkan pertumbuhan vegetatif paling baik dan total bobot kering bibit kakao yang secara nyata lebih tinggi jika dibandingkan dengan pemberian 100% dosis standar NPK-Mg kiserit. 

scholarly journals Efektivitas dolomit teraktivasi yang diperkaya dengan bakteri pelarut fosfat sebagai pengganti kiserit pada bibit kakao The effectiveness of activated dolomite enriched by phosphate solubilizing bacteria as kieserite substitute on cocoa seedling

2016 ◽  
Vol 80 (1) ◽  
Author(s):  
Laksmita Prima SANTI ◽  
Didiek Hadjar GOENADI
Keyword(s):  
Acid Soils ◽  
Dry Mass ◽  
Test Plant ◽  
Phosphate Solubilizing Bacteria ◽  
Pseudomonas Sp ◽  
Npk Fertilizers ◽  
Standard Dosage ◽  
Tropical Regions ◽  
Mg Deficiency ◽  
Phosphate Solubilizing

Abstract Magnesium (Mg) deficiency is commonly found in acid soils with high weathering intensity as widely present in tropical and sub-tropical regions, or those developed from low Mg-content parent materials. To overcome Mg deficiency, dolomite is often used to replace kieserite although the former is less reactive than the later. An activation technology by heating up to 800oC, followed by sulfic acid addition, then enriched by phosphate solubilizing bacteria is prospective to improve Mg solubility in soils. This research was aimed to enhance dolomite reactivity and to determine the effectiveness of activated dolomite enriched by phosphate solubilizing bacteria as Mg nutrient sources substituting kieserite on cocoa seedling growth. The results indicate that total and solubility in water of MgO from the prototype of activated dolomite were 26.7 and 9.2%, respectively. Using cocoa seedling as test plant, the use of activated dolomite enriched by using 109 cfu of Pseudomonas sp. at 3.85 g/seedling and combined with 100% dosages of NPK fertilizers showed the best vegetative growth and total dry mass of seedlings was significantly higher than that of 100% NPK-Mg kieserite standard dosage Abstrak Defisiensi Mg umumnya ditemukan pada tanah masam dengan tingkat pelapukan tinggi yang banyak terdapat di daerah tropik, subtropik, dan tanah yang terbentuk dari bahan induk miskin Mg. Untuk mengatasi defisiensi Mg, dolomit sering digunakan sebagai pengganti kiserit walaupun sifatnya kurang reaktif jika dibandingkan kiserit. Teknologi aktivasi dengan pemanasan mencapai 800oC, dilanjutkan dengan penambahan asam sulfat serta pengkayaan dengan bakteri pelarut fosfat diharapkan dapat meningkatkan kelarutan Mg di dalam tanah. Penelitian ini bertujuan untuk meningkatkan reaktivitas dolomit serta menetapkan efektivitas dolomit teraktivasi yang diperkaya dengan bakteri pelarut fosfat sebagai sumber hara Mg pengganti kieserit pada bibit kakao. Hasil yang diperoleh dari penelitian ini mengindikasikan bahwa kadar MgO total dan kelarutan dalam air pada dolomit teraktivasi masing-masing 26,7 dan 9,2%. Dengan menggunakan bibit kakao sebagai tanaman uji, aplikasi 3,85 g dolomit teraktivasi yang diperkaya dengan 109 cfu Pseudomonas sp./bibit yang dikombinasikan dengan 100% dosis pupuk NPK menunjukkan pertumbuhan vegetatif paling baik dan total bobot kering bibit kakao yang secara nyata lebih tinggi jika dibandingkan dengan pemberian 100% dosis standar NPK-Mg kiserit. 

scholarly journals Root Colonization and Growth Promotion of Soybean, Wheat and Chinese Cabbage byBacillus cereusYL6

2018 ◽  
Author(s):  
Yongli Ku ◽  
Guoyi Xu ◽  
Fawei Wang ◽  
Haijin Liu ◽  
Xiangna Yang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Chinese Cabbage ◽  
Fluorescent Protein ◽  
Growth Promotion ◽  
Organic Phosphorus ◽  
Root Surface ◽  
Available Phosphorus ◽  
Phosphate Solubilizing Bacteria ◽  
Soil Available Phosphorus ◽  
Phosphate Solubilizing

AbstractPhosphate-solubilizing bacteria (PSB) have been isolated and used in agricultural production. However, comprehensive research on PSB colonizing the rhizosphere of different plants and promoting plant growth is lacking. This study was conducted to study the growth-promoting effects and colonizing capacity of the PSB strain YL6. The YL6 strain not only increased the biomass of pot-planted soybean and wheat but also increased the yield and growth of Chinese cabbage under field conditions. The promotion of growth in these crops by strain YL6 was related to its capacities to dissolve inorganic and organic phosphorus and to produce a certain amount of indole-3-acetic (IAA) and gibberellin (GA). After YL6 was applied to soybean, wheat and Chinese cabbage, the rhizosphere soil available phosphorus (available P) content increased by 120.16%, 62.47% and 7.21%, respectively, and the plant total phosphorus increased by 198.60%, 6.20% and 78.89%, respectively, compared with those of plants without the addition of YL6. To determine whether the phosphate solubilizing bacteria colonized these plants, YL6 labeled with green fluorescent protein (YL6-GFP) was inoculated into plant rhizospheres. YL6-GFP first colonized the root surface and hairs and then penetrated into intercellular spaces and vessels. Collectively, these results demonstrate that YL6 promoted the growth of three different crops and colonized them in a similar way and therefore provide a solid foundation for probing into mechanisms by which phosphate-solubilizing bacteria affect plant growth.

KEBERADAAN BAKTERI PELARUT FOSFAT DAN AKTIVITAS ENZIM FOSFATASE TANAH DAERAH PERAKARAN TANAMAN OBAT DARI KEBUN RAYA CIBODAS

2018 ◽  
Vol 6 (3) ◽  
Author(s):  
Suliasih Suliasih
Keyword(s):  
Alkaline Phosphatase ◽  
Soil Sample ◽  
Rhizosphere Soil ◽  
Botanical Garden ◽  
Soil Enzyme ◽  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Bacillus Sp ◽  
Phosphate Solubilizing ◽  
Serratia Rubidaea

A study was undertaken to investigate to occurance of phosphate solubilizing bacteria from rhizosphere soil samples of medicine plants in Cibodas Botanical Garden. 13 soil samples of medicine plants are collected randomly The result shows that 71 isolates of phosphate solubilizing bacteria were isolated, and 10 species of these organism was identified as Azotobacter sp, Bacillus sp, Chromobacterium sp, C.violaceum, Citrobacter sp. , Enterobacter sp., E. liquefaciens. Nitrosomonas sp., Serratia rubidaea, Sphaerotillus natans. Azotobacter sp. And Bacillus sp. Are found in all of soil tested. Conversely, Serratia rubidaea is only in the sample from rhizosphere of Plantago mayor The activity of acid alkaline phosphatase in soil tested ranged from 0.78 – 60,18 ugp nitrophenole/g/h, with the higest values being recorded in soil sample from rhizosphere of “Lavender”.Keywords : phosphate solubilizing bacteria, soil enzyme phosphatase

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